The Experts below are selected from a list of 651 Experts worldwide ranked by ideXlab platform
Cheanyeh Cheng - One of the best experts on this subject based on the ideXlab platform.
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development of online sampling and matrix reduction technique coupled liquid chromatography ion trap mass spectrometry for determination Maduramicin in chicken meat
Food Chemistry, 2013Co-Authors: Kai Chun Chang, Cheanyeh ChengAbstract:Abstract An online sampling and matrix reduction technique coupled liquid chromatography electrospray–ion-trap mass spectrometry was developed for rapid analysis of Maduramicin (MAD) residue in chicken meat. Multiple-reaction monitoring of mass spectrometry in positive ion mode was used to detect Maduramicin. A post-column continuous infusion of internal standard (nigericin) with matrix-matched calibration method was utilised for quantification. The linear concentration range of the calibration curve was 0–10.0 ng mL−1 (r2 = 0.999). The limit of detection (quantification) was 0.08 ng g−1 (0.28 ng g−1). The analytical accuracy of chicken meat samples for four spiked MAD concentrations (0.5, 1.0, 5.0, and 10.0 ng g−1) was 84–97% and their corresponding intra-day and inter-day precisions were 3.7–5.0% and 5.8–7.9%, respectively. The analysis time for one sample was 10 min. The application of the method for incurred chicken samples elucidates that MAD residue in chicken meat decreases during the withdrawal period.
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Development of online sampling and matrix reduction technique coupled liquid chromatography/ion trap mass spectrometry for determination Maduramicin in chicken meat.
Food chemistry, 2013Co-Authors: Kai Chun Chang, Cheanyeh ChengAbstract:Abstract An online sampling and matrix reduction technique coupled liquid chromatography electrospray–ion-trap mass spectrometry was developed for rapid analysis of Maduramicin (MAD) residue in chicken meat. Multiple-reaction monitoring of mass spectrometry in positive ion mode was used to detect Maduramicin. A post-column continuous infusion of internal standard (nigericin) with matrix-matched calibration method was utilised for quantification. The linear concentration range of the calibration curve was 0–10.0 ng mL−1 (r2 = 0.999). The limit of detection (quantification) was 0.08 ng g−1 (0.28 ng g−1). The analytical accuracy of chicken meat samples for four spiked MAD concentrations (0.5, 1.0, 5.0, and 10.0 ng g−1) was 84–97% and their corresponding intra-day and inter-day precisions were 3.7–5.0% and 5.8–7.9%, respectively. The analysis time for one sample was 10 min. The application of the method for incurred chicken samples elucidates that MAD residue in chicken meat decreases during the withdrawal period.
Kai Chun Chang - One of the best experts on this subject based on the ideXlab platform.
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development of online sampling and matrix reduction technique coupled liquid chromatography ion trap mass spectrometry for determination Maduramicin in chicken meat
Food Chemistry, 2013Co-Authors: Kai Chun Chang, Cheanyeh ChengAbstract:Abstract An online sampling and matrix reduction technique coupled liquid chromatography electrospray–ion-trap mass spectrometry was developed for rapid analysis of Maduramicin (MAD) residue in chicken meat. Multiple-reaction monitoring of mass spectrometry in positive ion mode was used to detect Maduramicin. A post-column continuous infusion of internal standard (nigericin) with matrix-matched calibration method was utilised for quantification. The linear concentration range of the calibration curve was 0–10.0 ng mL−1 (r2 = 0.999). The limit of detection (quantification) was 0.08 ng g−1 (0.28 ng g−1). The analytical accuracy of chicken meat samples for four spiked MAD concentrations (0.5, 1.0, 5.0, and 10.0 ng g−1) was 84–97% and their corresponding intra-day and inter-day precisions were 3.7–5.0% and 5.8–7.9%, respectively. The analysis time for one sample was 10 min. The application of the method for incurred chicken samples elucidates that MAD residue in chicken meat decreases during the withdrawal period.
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Development of online sampling and matrix reduction technique coupled liquid chromatography/ion trap mass spectrometry for determination Maduramicin in chicken meat.
Food chemistry, 2013Co-Authors: Kai Chun Chang, Cheanyeh ChengAbstract:Abstract An online sampling and matrix reduction technique coupled liquid chromatography electrospray–ion-trap mass spectrometry was developed for rapid analysis of Maduramicin (MAD) residue in chicken meat. Multiple-reaction monitoring of mass spectrometry in positive ion mode was used to detect Maduramicin. A post-column continuous infusion of internal standard (nigericin) with matrix-matched calibration method was utilised for quantification. The linear concentration range of the calibration curve was 0–10.0 ng mL−1 (r2 = 0.999). The limit of detection (quantification) was 0.08 ng g−1 (0.28 ng g−1). The analytical accuracy of chicken meat samples for four spiked MAD concentrations (0.5, 1.0, 5.0, and 10.0 ng g−1) was 84–97% and their corresponding intra-day and inter-day precisions were 3.7–5.0% and 5.8–7.9%, respectively. The analysis time for one sample was 10 min. The application of the method for incurred chicken samples elucidates that MAD residue in chicken meat decreases during the withdrawal period.
Jiang Qinghui - One of the best experts on this subject based on the ideXlab platform.
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Study on resistance spectrum change of Eimeria tenella Qinhuangdao strain.
Chinese journal of veterinary science, 2010Co-Authors: Zhang Xiangzhai, Li Peiguo, Li Yunyu, Zhang Yanying, Jiang QinghuiAbstract:The resistance spectrum changes of Eimeria tenella(E.tenella) Qinhuangdao(QHD-08) strain in the 2004-2008 period were studied.One hundred and fifty four,14,days old,healthy chickens were divided randomly into 7 groups of 22 each,5 medicated groups,one positive group and one negative group.Based on the POAA,RLS,ROP and ACI,the drug resistances of E.tenella QHD strain were detected.The results revealed that the E.tenella QHD-08 strain was middle resistant to nicarbazin and complete resistant to amprolium,Maduramicin,keqiufen and ruiguanqiu.In the 2004-2008 period,resistance spectrum changes of E.tenella QHD strain to amprolium were non-resistant→light resistant→complete resistant.The resistances of E.tenella QHD strain changed from light resistant of 2006 to middle resistant of 2008 to nicarbazin,and light or middle resistant of 2006 to complete resistant of 2008 to Maduramicin,keqiufen,amprolium and ruiguanqiu.The results revealed that resistance spectrum of E.tenella strain to common anticoccidial drugs was broad,and resistance rate was increasing in recent years.
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Resistance of Eimeria tenella isolates from Qinhuangdao and Tangshan to seven anticoccidiosis drugs.
Chinese journal of veterinary science, 2009Co-Authors: Zhang Xiangzhai, Li Peiguo, Li Yunyu, Zhang Yanying, Jiang QinghuiAbstract:Three hundred and sixty 14-day-old healthy chickens were selected to study the resistance of Eimeria tenella(E.tenella) isolates(Qinhuangdao and Tangshan isolated) to keqiufen,Maduramicin,nicarbazin,amprolium,ruiguanqiu,jinsante and qiudi.The chickens were divided randomly into 18 groups of 20 each,in which of the two both isolates contained 7 medicated groups,one infected and one non-medicated groups,respectively.Based on the percent of optimum anticoccidial activity(POAA),reduction of lesion scores(RLS),relative oocyst production(ROP) and anticoccidial index(ACI),the results revealed that except E.tenella isolated from Qinhuangdao was sensitive to amprolium and nicarbazin,two E.tenella isolates(Qinhuangdao and Tangshan)were resistant to the seven drugs on different level,both of isolates were complete resistant to qiudi.It is suggested that Maduramicin and nicarbazin can be used in Tangshan,the others should not be used at present in Qinhuangdao and Tangshan.
Shanxiang Jiang - One of the best experts on this subject based on the ideXlab platform.
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Effect of Maduramicin on crayfish (Procambius clarkii): Hematological parameters, oxidative stress, histopathological changes and stress response.
Ecotoxicology and environmental safety, 2021Co-Authors: Xiuge Gao, Lin Peng, Yawei Qiu, Xinhao Song, Dawei Guo, Xiaoxiao Liu, Pei Teng, Shanxiang JiangAbstract:Abstract Maduramicin, an extensively used anticoccidial drug, has been introduced into environment due to poorly absorbed in the intestine of broiler chicken. To understand the potential ecological toxicity of Maduramicin on aquatic organisms, acute and subacute toxicity, hemolymph biochemistry, histopathology and the expressions of drug metabolism and stress response genes of crayfish (Procambius clarkii) were investigated in this study. For the first time, the 96 h median lethal concentration (LC50) of Maduramicin on crayfish was 67.03 mg L−1 with a 95% confidence interval (54.06–81.32 mg L−1). Then, the crayfish were exposed to 0.7 mg L−1 (1/100 LC50), 3.5 mg L−1 (1/20 LC50) and 7.0 mg L−1 (1/10 LC50) Maduramicin for 28 days. Maduramicin significantly altered biochemical parameters including AST, ALT, CK, LDH and ALP of hemolymph in crayfish at several time points. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) of crayfish gills, hepatopancreas and abdominal muscle were significantly decreased or elevated by different concentrations of Maduramicin treatment at varying time points. Furthermore, histopathological damage of crayfish gills, hepatopancreas and abdominal muscle were observed in a concentration-dependent manner. The expressions of metabolic and stress response genes (CYP450, GST, COX1, COX2, HSP70 and MT) in hepatopancreas of crayfish were significantly up-regulated by Maduramicin (7.0 mg L−1) treatment for 8 h to 7 d, and returned to normal levels after the removal of Maduramicin for 3–7 days. In conclusion, our findings demonstrated that environmental exposure of Maduramicin threaten to the health of crayfish living in the areas nearby livestock farms or pharmaceutical factory. Crayfish exhibited resistance to the stress of Maduramicin via activating drug metabolite and detoxification pathways.
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Ionophore Toxin Maduramicin Produces Haff Disease-Like Rhabdomyolysis in a Mouse Model.
International journal of environmental research and public health, 2020Co-Authors: Xiuge Gao, Lin Peng, Yawei Qiu, Dan Yang, Xinhao Song, Dawei Guo, Runan Zuo, Shanxiang JiangAbstract:Maduramicin is a toxic ionophore antibiotic that is isolated from Streptomyces, frequently occurring in an aquatic environment. To understand the potential role of Maduramicin in crayfish consumption related Haff disease, a mouse model was established in this study. Two exposure routes of Maduramicin in the abdominal muscle and the hepatopancreas tissue homogenates of crayfish were given intragastrically to mice in different doses for seven days. Action changes, clinical symptoms, feed consumption, body weight, blood biochemistry, and histopathology examination of mice were observed and analyzed. In the natural exposure group, relatively low concentration of Maduramicin in crayfish muscle and hepatopancreas had no obvious effects on mental state, body weight, blood biochemical indexes, or histologic appearance. However, in the artificial exposure group, with increasing concentrations, Maduramicin in crayfish muscle and hepatopancreas homogenates both induced mental sluggishness and weight loss of mice. Blood biochemical examination showed that 3.5 mg·kg−1 and 7 mg·kg−1 Maduramicin in crayfish tissue homogenates significantly increased levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), and creatine kinase (CK). Additionally, histopathological examination showed that multiple organs were damaged by Maduramicin, including degeneration of liver cells, shedding of renal epithelial cells, and disturbance and partial lysis of myocardial and skeletal muscle filaments in the mice. In summary, Maduramicin may not cause Haff disease through contamination of the aquatic environment under normal conditions. Maduramicin can be used as a potential toxin tool to establish a rhabdomyolysis disease animal model for drug development.
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Maduramicin triggers methuosis-like cell death in primary chicken myocardial cells.
Toxicology letters, 2020Co-Authors: Xiuge Gao, Xiangchun Ruan, Lin Peng, Yawei Qiu, Dan Yang, Xinhao Song, Dawei Guo, Shanxiang JiangAbstract:Abstract Maduramicin frequently induces severe cardiotoxicity in broiler chickens as well as in humans who consume Maduramicin accidentally. Apoptosis and non-apoptotic cell death occur concurrently in the process of Maduramicin-induced cardiotoxicity; however, the underlying mechanism of non-apoptotic cell death is largely unknown. Here, we report the relationship between Maduramicin-caused cytoplasmic vacuolization and methuosis-like cell death as well as the underlying mechanism in primary chicken myocardial cells. Maduramicin induced a significant increase of cytoplasmic vacuoles with a degree of cell specificity in primary chicken embryo fibroblasts and chicken hepatoma cells (LMH), along with a decrease of ATP and an increase of LDH. The accumulated vacuoles were partly derived from cellular endocytosis rather than the swelling of endoplasm reticulum, lysosomes, and mitochondria. Moreover, the broad-spectrum caspase inhibitor carbobenzoxy-Val-Ala-Asp-fluoromethylketone (z-VAD-fmk) did not prevent Maduramicin-induced cytoplasmic vacuolization. DNA ladder and cleavage of PARP were not observed in chicken myocardial cells during Maduramicin exposure. Pretreatment with 3-methyladenine (3-MA) and cholorquine (CQ) of chicken myocardial cells did not attenuate cytoplasmic vacuolization and cytotoxicity, although LC3 and p62 were activated. Bafilomycin A1 almost completely prevented the generation of cytoplasmic vacuoles and significantly attenuated cytotoxicity induced by Maduramicin, along with downregulation of K-Ras and upregulation of Rac1. Taken together, “methuosis” due to excessive cytoplasmic vacuolization mediates the cardiotoxicity of Maduramicin. This provides new insights for understanding a nonclassical form of cell death in the field of drug-induced cytotoxicity.
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Maduramicin induces apoptosis through ROS-PP5-JNK pathway in skeletal myoblast cells and muscle tissue.
Toxicology, 2019Co-Authors: Xin Chen, Yumei Zhang, Shanxiang Jiang, Shile HuangAbstract:Abstract Our previous work has shown that Maduramicin, an effective coccidiostat used in the poultry production, executed its toxicity by inducing apoptosis of skeletal myoblasts. However, the underlying mechanism is not well understood. Here we show that Maduramicin induced apoptosis of skeletal muscle cells by activating c-Jun N-terminal kinase (JNK) pathway in murine C2C12 and L6 myoblasts as well as skeletal muscle tissue. This is supported by the findings that inhibition of JNK with SP600125 or ectopic expression of dominant negative c-Jun attenuated Maduramicin-induced apoptosis in C2C12 cells. Furthermore, we found that treatment with Maduramicin reduced the cellular protein level of protein phosphatase 5 (PP5). Overexpression of PP5 substantially mitigated Maduramicin-activated JNK and apoptosis. Moreover, we noticed that treatment with Maduramicin elevated intracellular reactive oxygen species (ROS) level. Pretreatment with N-acetyl-L-cysteine (NAC), a ROS scavenger and antioxidant, suppressed Maduramicin-induced inhibition of PP5 and activation of JNK as well as apoptosis. The results indicate that Maduramicin induction of ROS inhibits PP5, which results in activation of JNK cascade, leading to apoptosis of skeletal muscle cells. Our finding suggests that manipulation of ROS-PP5-JNK pathway may be a potential approach to prevent Maduramicin-induced apoptotic cell death in skeletal muscle.
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Effects of Maduramicin on adult zebrafish (Danio rerio): Acute toxicity, tissue damage and oxidative stress.
Ecotoxicology and environmental safety, 2018Co-Authors: Lin Peng, Xiuge Gao, Shanxiang JiangAbstract:Abstract Maduramicin, a potent polyether ionophore antibiotic, has been widely used to control coccidiosis in the poultry production. Nevertheless, incomplete metabolism of Maduramicin in chicken may result in its accumulation in the aquatic environment, while Maduramicin's threat to fish remains largely unknown. In the present study, we focused on acute toxicity, histopathological lesion and oxidative stress damage of Maduramicin in adult zebrafish. Primarily, we obtained that the 96-h median lethal concentration (96 h LC50) of adult zebrafish exposure to Maduramicin was 13.568 mg/L. On basis of that, adult zebrafish were separately exposed to 0.1 mg/L (1/125 LC50), 0.5 mg/L (1/25 LC50) and 2.5 mg/L (1/5 LC50) Maduramicin for 14 days. On day 3, 0.1 mg/L Maduramicin significantly increased the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione s-transferase (GST) in the liver of zebrafish, while the activities of these antioxidant enzymes in the liver were significantly inhibited by 2.5 mg/L Maduramicin. Moreover, the contents of malondialdehyde (MDA) in the liver of different dose groups were all significantly promoted after 14 days of exposure. For the gill of zebrafish, the increase in MDA contents was found after only 3 days of exposure to Maduramicin. Furthermore, Maduramicin treatment significantly up-regulated the mRNA levels of genes (sod1, gpx1a, gstr, nrf2 and keap1) in the liver of zebrafish after 3 days of exposure. On days 6, 9 and 14, Maduramicin treatment significantly down-regulated the mRNA levels of these genes in the liver of zebrafish. Meanwhile, Maduramicin significantly down-regulated the mRNA levels of genes (sod1, cat, gpx1a, gstr, nrf2 and keap1) in the gill of zebrafish during the 14-day of exposure. In addition, a dose-dependent induction in histopathological lesion was observed in multiple organs after 14 days of exposure, including lamellar fusion, epithelial lifting in the gill and vacuole formation in the liver as well as the fracture of intestinal villus in the intestine. Taken together, our findings demonstrated that waterborne Maduramicin (2.5 mg/L) exposure can induce severe oxidative stress and tissue damage in adult zebrafish while this damage was not enough to kill them after 14 days of waterborne exposure.
Zhang Xiangzhai - One of the best experts on this subject based on the ideXlab platform.
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Study on resistance spectrum change of Eimeria tenella Qinhuangdao strain.
Chinese journal of veterinary science, 2010Co-Authors: Zhang Xiangzhai, Li Peiguo, Li Yunyu, Zhang Yanying, Jiang QinghuiAbstract:The resistance spectrum changes of Eimeria tenella(E.tenella) Qinhuangdao(QHD-08) strain in the 2004-2008 period were studied.One hundred and fifty four,14,days old,healthy chickens were divided randomly into 7 groups of 22 each,5 medicated groups,one positive group and one negative group.Based on the POAA,RLS,ROP and ACI,the drug resistances of E.tenella QHD strain were detected.The results revealed that the E.tenella QHD-08 strain was middle resistant to nicarbazin and complete resistant to amprolium,Maduramicin,keqiufen and ruiguanqiu.In the 2004-2008 period,resistance spectrum changes of E.tenella QHD strain to amprolium were non-resistant→light resistant→complete resistant.The resistances of E.tenella QHD strain changed from light resistant of 2006 to middle resistant of 2008 to nicarbazin,and light or middle resistant of 2006 to complete resistant of 2008 to Maduramicin,keqiufen,amprolium and ruiguanqiu.The results revealed that resistance spectrum of E.tenella strain to common anticoccidial drugs was broad,and resistance rate was increasing in recent years.
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Resistance of Eimeria tenella isolates from Qinhuangdao and Tangshan to seven anticoccidiosis drugs.
Chinese journal of veterinary science, 2009Co-Authors: Zhang Xiangzhai, Li Peiguo, Li Yunyu, Zhang Yanying, Jiang QinghuiAbstract:Three hundred and sixty 14-day-old healthy chickens were selected to study the resistance of Eimeria tenella(E.tenella) isolates(Qinhuangdao and Tangshan isolated) to keqiufen,Maduramicin,nicarbazin,amprolium,ruiguanqiu,jinsante and qiudi.The chickens were divided randomly into 18 groups of 20 each,in which of the two both isolates contained 7 medicated groups,one infected and one non-medicated groups,respectively.Based on the percent of optimum anticoccidial activity(POAA),reduction of lesion scores(RLS),relative oocyst production(ROP) and anticoccidial index(ACI),the results revealed that except E.tenella isolated from Qinhuangdao was sensitive to amprolium and nicarbazin,two E.tenella isolates(Qinhuangdao and Tangshan)were resistant to the seven drugs on different level,both of isolates were complete resistant to qiudi.It is suggested that Maduramicin and nicarbazin can be used in Tangshan,the others should not be used at present in Qinhuangdao and Tangshan.
