The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform
Bernhard Ryffel - One of the best experts on this subject based on the ideXlab platform.
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interferon λ and interleukin 22 act synergistically for the induction of interferon stimulated genes and control of rotavirus infection
Nature Immunology, 2015Co-Authors: Pedro P Hernandez, Tanel Mahlakoiv, Ines Yang, Vera Schwierzeck, Nam Nguyen, Fabian Guendel, Konrad Gronke, Bernhard RyffelAbstract:The epithelium is the Main Entry Point for many viruses, but the processes that protect barrier surfaces against viral infections are incompletely understood. Here we identified interleukin 22 (IL-22) produced by innate lymphoid cell group 3 (ILC3) as an amplifier of signaling via interferon-λ (IFN-λ), a synergism needed to curtail the replication of rotavirus, the leading cause of childhood gastroenteritis. Cooperation between the receptor for IL-22 and the receptor for IFN-λ, both of which were 'preferentially' expressed by intestinal epithelial cells (IECs), was required for optimal activation of the transcription factor STAT1 and expression of interferon-stimulated genes (ISGs). These data suggested that epithelial cells are protected against viral replication by co-option of two evolutionarily related cytokine networks. These data may inform the design of novel immunotherapy for viral infections that are sensitive to interferons.
Pedro P Hernandez - One of the best experts on this subject based on the ideXlab platform.
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interferon λ and interleukin 22 act synergistically for the induction of interferon stimulated genes and control of rotavirus infection
Nature Immunology, 2015Co-Authors: Pedro P Hernandez, Tanel Mahlakoiv, Ines Yang, Vera Schwierzeck, Nam Nguyen, Fabian Guendel, Konrad Gronke, Bernhard RyffelAbstract:The epithelium is the Main Entry Point for many viruses, but the processes that protect barrier surfaces against viral infections are incompletely understood. Here we identified interleukin 22 (IL-22) produced by innate lymphoid cell group 3 (ILC3) as an amplifier of signaling via interferon-λ (IFN-λ), a synergism needed to curtail the replication of rotavirus, the leading cause of childhood gastroenteritis. Cooperation between the receptor for IL-22 and the receptor for IFN-λ, both of which were 'preferentially' expressed by intestinal epithelial cells (IECs), was required for optimal activation of the transcription factor STAT1 and expression of interferon-stimulated genes (ISGs). These data suggested that epithelial cells are protected against viral replication by co-option of two evolutionarily related cytokine networks. These data may inform the design of novel immunotherapy for viral infections that are sensitive to interferons.
Jochen Garcke - One of the best experts on this subject based on the ideXlab platform.
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Advancing a Gateway Infrastructure for Wind Turbine Data Analysis
Journal of Grid Computing, 2016Co-Authors: Alvaro Aguilera, Johannes Luong, Dirk Schollbach, Ulf Markwardt, Richard Grunzke, Dirk Habich, Jochen GarckeAbstract:The increasing amount of data produced in many scientific and engineering doMains creates as many new challenges for an efficient data analysis, as possibilities for its application. In this paper, we present one of the use cases of the project VAVID, namely the condition monitoring of sensor information from wind turbines, and how a data gateway can help to increase the usability and security of the proposed system. Starting by briefly introducing the project, the paper presents the problem of handling and processing large amount of sensor data using existing tools in the context of wind turbines. It goes on to describe the innovative approach used in VAVID to meet this challenge, covering the Main goals, numerical methods used for analysis, the storage concept, and the architectural design. It concludes by offering a rational for the use of a data gateway as the Main Entry Point to the system and how this is being implemented in VAVID.
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Towards an Industry Data Gateway: An Integrated Platform for the Analysis of Wind Turbine Data
2015 7th International Workshop on Science Gateways, 2015Co-Authors: Alvaro Aguilera, Dirk Schollbach, Ulf Markwardt, Richard Grunzke, Dirk Habich, Jochen GarckeAbstract:The increasing amount of data produced in many scientific and engineering doMains creates as many new challenges for an efficient data analysis, as possibilities for its application. In this paper, we present one of the use-cases of the project VAVID, namely the condition monitoring of sensor information from wind turbines, and how a data gateway can help to increase the usability and security of the proposed system. Starting by briefly introducing the project, the paper presents the problem of handling and processing large amount of sensor data using existing tools in the context of wind turbines. It goes on to describe the innovative approach used in VAVID to meet this challenge, covering the Main goals, numerical methods used for analysis, the storage concept, and architectural design. It concludes by offering a rational for the use of a data gateway as the Main Entry Point to the system and how this is being implemented in VAVID.
G Foffani - One of the best experts on this subject based on the ideXlab platform.
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Spinal direct current stimulation modulates the activity of gracile nucleus and primary somatosensory cortex in anaesthetized rats.
The Journal of Physiology, 2011Co-Authors: J Aguilar, F Pulecchi, R Dilena, A Oliviero, G FoffaniAbstract:Non-Technical Summary Stimulation of the human brain with direct current is a simple but effective neuromodulation technique that is becoming increasingly popular due to its potentiality for non-invasively treating a variety of neurological and neuropsychiatric disorders. Recently, this neuromodulation technique has been extended to the stimulation of the human spinal cord. Here we investigated the mechanisms of action of spinal direct current stimulation (sDCS) in anaesthetized rats. We found that sDCS can selectively modulate the spontaneous activity entering the brain through the spinal cord via the somatosensory system, consequently modulating both the internal state of the brain and its responsiveness to external somatosensory stimuli. These findings have at least two levels of significance: from a physiological perspective, they remark on the importance of the spinal cord in regulating the state of the brain; from a clinical perspective, they offer a mechanistic rationale for the development of sDCS as an effective bottom-up neuromodulation technique. Abstract Afferent somatosensory activity from the spinal cord has a profound impact on the activity of the brain. Here we investigated the effects of spinal stimulation using direct current, delivered at the thoracic level, on the spontaneous activity and on the somatosensory evoked potentials of the gracile nucleus, which is the Main Entry Point for hindpaw somatosensory signals reaching the brain from the dorsal columns, and of the primary somatosensory cortex in anaesthetized rats. Anodal spinal direct current stimulation (sDCS) increased the spontaneous activity and decreased the amplitude of evoked responses in the gracile nucleus, whereas cathodal sDCS produced the opposite effects. At the level of the primary somatosensory cortex, the changes in spontaneous activity induced by sDCS were consistent with the effects observed in the gracile nucleus, but the changes in cortical evoked responses were more variable and state dependent. Therefore, sDCS can modulate in a polarity-specific manner the supraspinal activity of the somatosensory system, offering a versatile bottom-up neuromodulation technique that could potentially be useful in a number of clinical applications.
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Spinal direct current stimulation modulates the activity of gracile nucleus and primary somatosensory cortex in anaesthetized rats.
The Journal of physiology, 2011Co-Authors: J Aguilar, F Pulecchi, R Dilena, A Oliviero, A Priori, G FoffaniAbstract:Afferent somatosensory activity from the spinal cord has a profound impact on the activity of the brain. Here we investigated the effects of spinal stimulation using direct current, delivered at the thoracic level, on the spontaneous activity and on the somatosensory evoked potentials of the gracile nucleus, which is the Main Entry Point for hindpaw somatosensory signals reaching the brain from the dorsal columns, and of the primary somatosensory cortex in anaesthetized rats. Anodal spinal direct current stimulation (sDCS) increased the spontaneous activity and decreased the amplitude of evoked responses in the gracile nucleus, whereas cathodal sDCS produced the opposite effects. At the level of the primary somatosensory cortex, the changes in spontaneous activity induced by sDCS were consistent with the effects observed in the gracile nucleus, but the changes in cortical evoked responses were more variable and state dependent. Therefore, sDCS can modulate in a polarity-specific manner the supraspinal activity of the somatosensory system, offering a versatile bottom-up neuromodulation technique that could potentially be useful in a number of clinical applications.
Gabriel Roblin - One of the best experts on this subject based on the ideXlab platform.
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Early Sr2+-induced effects on membrane potential, proton pumping- and ATP hydrolysis-activities of plasma membrane vesicles from maize root cells
Environmental and Experimental Botany, 2020Co-Authors: Christelle Moyen, Janine Bonmort, Gabriel RoblinAbstract:International audienceWhen released in plant environment, strontium (Sr2+) can be absorbed predominantly by the plant roots. As the plasma membrane of root cells is amongst the first barriers encountered by Sr2+ during its soil/plant transfer and the Main Entry Point of Sr2+ into the roots, the Main objective of this work aimed to enlighten on some of the Sr2+-induced effects at this level in Zea mays L. cv. "Liberal". Thus this study focused on the Sr2+-induced changes on membrane potential of cortical root cells and on proton fluxes in maize roots, in order to determine whether the activity of some of the ion transport systems present in the plasma membrane of maize root cell could be among the first targets of Sr2+. We focused in particular on the plasma membrane H+-ATPase, known to be one of the major transport systems found in the plasmalemma where it generates a proton motive force (contributing to membrane potential Maintaining, and providing energy for ion transport through membrane). The data presented here showed that Sr2+ triggered an early and transient membrane depolarisation whose magnitude and duration were dependent on the Sr2+-concentration. The time course pattern of a second longer lasting depolarisation could be examined in perspective with the Sr2+-induced decrease of the spontaneous proton extrusion observed in root tissues, suggesting a relationship between Sr2+- effects on membrane potential and H+ excretion. Furthermore, the inhibitory effect exerted by Sr2+ on the fusicoccin (FC)-enhanced proton extrusion strongly suggested an inhibition of the plasma membrane H+-ATPase. This hypothesis was supported by the inhibition induced by Sr2+ on proton pumping- and ATP hydrolysis-activities measured in plasma membrane vesicles (PMV) prepared from maize roots. Taken together the data reported here evidence that, with however a lower efficiency, Sr2+ behaved in a quite similar way to Ca2+ when inhibiting the H+-ATPase activity, and suggest that Sr2+ could partially mimic Ca2+ onto regulation of the H+-ATPase activity
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Early Sr2+-induced effects on membrane potential, proton pumping- and ATP hydrolysis-activities of plasma membrane vesicles from maize root cells
Environmental and Experimental Botany, 2011Co-Authors: Christelle Moyen, Janine Bonmort, Gabriel RoblinAbstract:Abstract When released in plant environment, strontium (Sr 2+ ) can be absorbed predominantly by the plant roots. As the plasma membrane of root cells is amongst the first barriers encountered by Sr 2+ during its soil/plant transfer and the Main Entry Point of Sr 2+ into the roots, the Main objective of this work aimed to enlighten on some of the Sr 2+ -induced effects at this level in Zea mays L. cv. “Liberal”. Thus this study focused on the Sr 2+ -induced changes on membrane potential of cortical root cells and on proton fluxes in maize roots, in order to determine whether the activity of some of the ion transport systems present in the plasma membrane of maize root cell could be among the first targets of Sr 2+ . We focused in particular on the plasma membrane H + -ATPase, known to be one of the major transport systems found in the plasmalemma where it generates a proton motive force (contributing to membrane potential Maintaining, and providing energy for ion transport through membrane). The data presented here showed that Sr 2+ triggered an early and transient membrane depolarisation whose magnitude and duration were dependent on the Sr 2+ -concentration. The time course pattern of a second longer lasting depolarisation could be examined in perspective with the Sr 2+ -induced decrease of the spontaneous proton extrusion observed in root tissues, suggesting a relationship between Sr 2+ -effects on membrane potential and H + excretion. Furthermore, the inhibitory effect exerted by Sr 2+ on the fusicoccin (FC)-enhanced proton extrusion strongly suggested an inhibition of the plasma membrane H + -ATPase. This hypothesis was supported by the inhibition induced by Sr 2+ on proton pumping- and ATP hydrolysis-activities measured in plasma membrane vesicles (PMV) prepared from maize roots. Taken together the data reported here evidence that, with however a lower efficiency, Sr 2+ behaved in a quite similar way to Ca 2+ when inhibiting the H + -ATPase activity, and suggest that Sr 2+ could partially mimic Ca 2+ onto regulation of the H + -ATPase activity.
