The Experts below are selected from a list of 2523 Experts worldwide ranked by ideXlab platform

Ismayil Safa Gurcan - One of the best experts on this subject based on the ideXlab platform.

  • cytotoxic effects of etephon and Maleic Hydrazide in vero hep2 hepg2 cells
    Drug and Chemical Toxicology, 2014
    Co-Authors: Begum Yurdakok, Emine Baydan, Hamza Okur, Ismayil Safa Gurcan
    Abstract:

    AbstractThe toxicity of etephon and Maleic Hydrazide, used as plant growth regulators in agriculture, were reported as low in mammals in previous studies. However, in vitro cytotoxicity studies in mammalian cells are currently missing to understand their toxicity at molecular level. In the current study, the cytotoxicity of these compounds, were studied in Vero (African green monkey kidney epithelium), HepG2 (human hepatocellular carcinoma), Hep2 (human epidermoid cancer) cells by MTT ((3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromure) and LDH (lactate dehydrogenase) assays. Maleic Hydrazide had lower IC50 values for all cell lines compared to ethephon. Least cytotoxic effect treated by ethephon were observed in Vero, followed by HepG2 and Hep2. Similarly Maleic Hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells (p < 0.05). IC50 values in general were found to be highest in Vero cells, followed by HepG2 and Hep2 cells (p < 0.05). LDH and MTT assays showed cor...

  • Cytotoxic effects of etephon and Maleic Hydrazide in Vero, Hep2, HepG2 cells
    Drug and Chemical Toxicology, 2014
    Co-Authors: Begum Yurdakok, Emine Baydan, Hamza Okur, Ismayil Safa Gurcan
    Abstract:

    AbstractThe toxicity of etephon and Maleic Hydrazide, used as plant growth regulators in agriculture, were reported as low in mammals in previous studies. However, in vitro cytotoxicity studies in mammalian cells are currently missing to understand their toxicity at molecular level. In the current study, the cytotoxicity of these compounds, were studied in Vero (African green monkey kidney epithelium), HepG2 (human hepatocellular carcinoma), Hep2 (human epidermoid cancer) cells by MTT ((3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromure) and LDH (lactate dehydrogenase) assays. Maleic Hydrazide had lower IC50 values for all cell lines compared to ethephon. Least cytotoxic effect treated by ethephon were observed in Vero, followed by HepG2 and Hep2. Similarly Maleic Hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells (p 

Begum Yurdakok - One of the best experts on this subject based on the ideXlab platform.

  • cytotoxic effects of etephon and Maleic Hydrazide in vero hep2 hepg2 cells
    Drug and Chemical Toxicology, 2014
    Co-Authors: Begum Yurdakok, Emine Baydan, Hamza Okur, Ismayil Safa Gurcan
    Abstract:

    AbstractThe toxicity of etephon and Maleic Hydrazide, used as plant growth regulators in agriculture, were reported as low in mammals in previous studies. However, in vitro cytotoxicity studies in mammalian cells are currently missing to understand their toxicity at molecular level. In the current study, the cytotoxicity of these compounds, were studied in Vero (African green monkey kidney epithelium), HepG2 (human hepatocellular carcinoma), Hep2 (human epidermoid cancer) cells by MTT ((3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromure) and LDH (lactate dehydrogenase) assays. Maleic Hydrazide had lower IC50 values for all cell lines compared to ethephon. Least cytotoxic effect treated by ethephon were observed in Vero, followed by HepG2 and Hep2. Similarly Maleic Hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells (p < 0.05). IC50 values in general were found to be highest in Vero cells, followed by HepG2 and Hep2 cells (p < 0.05). LDH and MTT assays showed cor...

  • Cytotoxic effects of etephon and Maleic Hydrazide in Vero, Hep2, HepG2 cells
    Drug and Chemical Toxicology, 2014
    Co-Authors: Begum Yurdakok, Emine Baydan, Hamza Okur, Ismayil Safa Gurcan
    Abstract:

    AbstractThe toxicity of etephon and Maleic Hydrazide, used as plant growth regulators in agriculture, were reported as low in mammals in previous studies. However, in vitro cytotoxicity studies in mammalian cells are currently missing to understand their toxicity at molecular level. In the current study, the cytotoxicity of these compounds, were studied in Vero (African green monkey kidney epithelium), HepG2 (human hepatocellular carcinoma), Hep2 (human epidermoid cancer) cells by MTT ((3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromure) and LDH (lactate dehydrogenase) assays. Maleic Hydrazide had lower IC50 values for all cell lines compared to ethephon. Least cytotoxic effect treated by ethephon were observed in Vero, followed by HepG2 and Hep2. Similarly Maleic Hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells (p 

Manuel Chicharro - One of the best experts on this subject based on the ideXlab platform.

  • Determination of Maleic Hydrazide in Potato Samples Using Capillary Electrophoresis with Dual Detection (UV-Electrochemical)
    Electroanalysis, 2008
    Co-Authors: Manuel Chicharro, Esperanza Bermejo, Sara Ongay, Antonio Zapardiel
    Abstract:

    A new method for the separation of a mixture of different herbicides (propham, chlorpropham, asulam, metamitron, linuron, and Maleic Hydrazide) using MEKC is proposed. A base-line separation for the mixture of herbicides is achieved in less than six minutes. The detection limits obtained for all the herbicides were lower than 1.0 μM using UV detection. This separation method was used for the determination of Maleic Hydrazide in potato samples. If a dual (UV-electrochemical) detection system is employed, chlorpropham can be also detected. The results obtained showed that electrochemical detection was ten folds more sensitive than UV detection for Maleic Hydrazide. The detection limit of the proposed method for Maleic Hydrazide employing electrochemical detection was 1.3 μg g−1, this value is lower than 50 μg g−1, which is the maximum residue level permitted for this plant growth regulator in potato samples. The results obtained in the work clearly demonstrate the advantage of using electrochemical detection coupled to capillary electrophoresis, using this detection the concentration limits are not compromised by miniaturization and the components required are simple and inexpensive.

  • flow injection analysis of Maleic Hydrazide using an electrochemical sensor based on palladium dispersed carbon paste electrode
    Electroanalysis, 2007
    Co-Authors: Alberto Sanchez, Esperanza Bermejo, Antonio Zapardiel, Lopez F De Prado, Monica Moreno, J A Perezlopez, Manuel Chicharro
    Abstract:

    A new analytical methodology for the electrochemical detection of the herbicide Maleic Hydrazide (3,6-dihydroxypyridazine) by flow injection analysis is presented. This method is supported by the novel application of a palladium-dispersed carbon paste electrode as an amperometric sensor for this herbicide. Maleic Hydrazide shows anodic electrochemical activity on carbon-based electrodes (glassy carbon or carbon paste electrodes) in all the pH range. This electrochemical activity is enhanced using metal-dispersed carbon paste electrodes, especially at Pd-dispersed CPE which displays good oxidation signals at 690 mV (0.050 M phosphate buffer pH 7.0), 140 mV lower than at unmodified electrodes. Under the optimized conditions, the electroanalytical performance of Pd-dispersed CPE in flow injection analysis was excellent, with good reproducibility (RSD 3.3%) and a wide linear range (1.9×10−7 to 1.0×10−4 mol L−1). A detection limit of 1.4×10−8 mol L−1 (0.14 ng Maleic Hydrazide) was obtained for a sample loop of 100 μL at a fixed potential of 700 mV in 0.050 M phosphate buffer solution at pH 7.0 and a flow rate of 2.0 mL min−1. The proposed method was applied for the Maleic Hydrazide detection in natural drinking water samples.

Emine Baydan - One of the best experts on this subject based on the ideXlab platform.

  • cytotoxic effects of etephon and Maleic Hydrazide in vero hep2 hepg2 cells
    Drug and Chemical Toxicology, 2014
    Co-Authors: Begum Yurdakok, Emine Baydan, Hamza Okur, Ismayil Safa Gurcan
    Abstract:

    AbstractThe toxicity of etephon and Maleic Hydrazide, used as plant growth regulators in agriculture, were reported as low in mammals in previous studies. However, in vitro cytotoxicity studies in mammalian cells are currently missing to understand their toxicity at molecular level. In the current study, the cytotoxicity of these compounds, were studied in Vero (African green monkey kidney epithelium), HepG2 (human hepatocellular carcinoma), Hep2 (human epidermoid cancer) cells by MTT ((3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromure) and LDH (lactate dehydrogenase) assays. Maleic Hydrazide had lower IC50 values for all cell lines compared to ethephon. Least cytotoxic effect treated by ethephon were observed in Vero, followed by HepG2 and Hep2. Similarly Maleic Hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells (p < 0.05). IC50 values in general were found to be highest in Vero cells, followed by HepG2 and Hep2 cells (p < 0.05). LDH and MTT assays showed cor...

  • Cytotoxic effects of etephon and Maleic Hydrazide in Vero, Hep2, HepG2 cells
    Drug and Chemical Toxicology, 2014
    Co-Authors: Begum Yurdakok, Emine Baydan, Hamza Okur, Ismayil Safa Gurcan
    Abstract:

    AbstractThe toxicity of etephon and Maleic Hydrazide, used as plant growth regulators in agriculture, were reported as low in mammals in previous studies. However, in vitro cytotoxicity studies in mammalian cells are currently missing to understand their toxicity at molecular level. In the current study, the cytotoxicity of these compounds, were studied in Vero (African green monkey kidney epithelium), HepG2 (human hepatocellular carcinoma), Hep2 (human epidermoid cancer) cells by MTT ((3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromure) and LDH (lactate dehydrogenase) assays. Maleic Hydrazide had lower IC50 values for all cell lines compared to ethephon. Least cytotoxic effect treated by ethephon were observed in Vero, followed by HepG2 and Hep2. Similarly Maleic Hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells (p 

Hamza Okur - One of the best experts on this subject based on the ideXlab platform.

  • cytotoxic effects of etephon and Maleic Hydrazide in vero hep2 hepg2 cells
    Drug and Chemical Toxicology, 2014
    Co-Authors: Begum Yurdakok, Emine Baydan, Hamza Okur, Ismayil Safa Gurcan
    Abstract:

    AbstractThe toxicity of etephon and Maleic Hydrazide, used as plant growth regulators in agriculture, were reported as low in mammals in previous studies. However, in vitro cytotoxicity studies in mammalian cells are currently missing to understand their toxicity at molecular level. In the current study, the cytotoxicity of these compounds, were studied in Vero (African green monkey kidney epithelium), HepG2 (human hepatocellular carcinoma), Hep2 (human epidermoid cancer) cells by MTT ((3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromure) and LDH (lactate dehydrogenase) assays. Maleic Hydrazide had lower IC50 values for all cell lines compared to ethephon. Least cytotoxic effect treated by ethephon were observed in Vero, followed by HepG2 and Hep2. Similarly Maleic Hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells (p < 0.05). IC50 values in general were found to be highest in Vero cells, followed by HepG2 and Hep2 cells (p < 0.05). LDH and MTT assays showed cor...

  • Cytotoxic effects of etephon and Maleic Hydrazide in Vero, Hep2, HepG2 cells
    Drug and Chemical Toxicology, 2014
    Co-Authors: Begum Yurdakok, Emine Baydan, Hamza Okur, Ismayil Safa Gurcan
    Abstract:

    AbstractThe toxicity of etephon and Maleic Hydrazide, used as plant growth regulators in agriculture, were reported as low in mammals in previous studies. However, in vitro cytotoxicity studies in mammalian cells are currently missing to understand their toxicity at molecular level. In the current study, the cytotoxicity of these compounds, were studied in Vero (African green monkey kidney epithelium), HepG2 (human hepatocellular carcinoma), Hep2 (human epidermoid cancer) cells by MTT ((3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromure) and LDH (lactate dehydrogenase) assays. Maleic Hydrazide had lower IC50 values for all cell lines compared to ethephon. Least cytotoxic effect treated by ethephon were observed in Vero, followed by HepG2 and Hep2. Similarly Maleic Hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells (p