The Experts below are selected from a list of 1134 Experts worldwide ranked by ideXlab platform
Seyed Mohammad Taghi Gharibzahedi - One of the best experts on this subject based on the ideXlab platform.
-
Cellulase-assisted extraction of polysaccharides from Malva sylvestris: Process optimization and potential functionalities
International journal of biological macromolecules, 2017Co-Authors: Hosein Rostami, Seyed Mohammad Taghi GharibzahediAbstract:Abstract Enzyme-assisted extraction process of the water-soluble Malva sylvestris polysaccharides (MSPs) was optimized using response surface methodology (RSM) . The highest yield (10.40%) of MSPs was achieved at 5.64% cellulase, 55.65 °C temperature, 3.4 h time, and 5.22 pH. Three homogeneous polysaccharide fractions (MSP-1, MSP-2, MSP-3) were purified by DEAE-cellulose and Sephadex G-100 chromatography, which were composed of galactose, glucuronic acid, arabinose, rhamnose and mannose in different molar ratios with molecular weight range of 2.6 × 10 5 –8.8 × 10 5 Da. The fractions could significantly increase antioxidant, antitumor and antimicrobial activities in a dose-dependent pattern. MSP-2 revealed stronger antioxidant activities than MSP-1 and MSP-3, including reducing power and scavenging activity of DPPH and OH radicals. The antiproliferative activity of MSP-2 (1.0 mg/mL) on the growth of A549 and HepG2 cells was 45.1% and 53.2%, respectively. The Gram-positive bacteria ( Bacillus cereus PTCC 1015 and Staphylococcus aureus PTCC 1112) compared with Gram-negative ones ( Escherichia coli PTCC 1763 and Salmonella typhimurium PTCC 1709) showed less sensitivity against the various MSPs (3–15 mg/mL).
Abdollah Ghasemi Pirbalouti - One of the best experts on this subject based on the ideXlab platform.
-
Wound Healing Activity of Extracts of Malva sylvestris and Stachys lavandulifolia
International Journal of Biology, 2010Co-Authors: Abdollah Ghasemi Pirbalouti, Abed KoohpyehAbstract:The flowers of Stachys lavandulifolia Vahl (Lamiaceae) and Malva sylvestris Linn (Malvaceae) are traditionally used to treat various skin disorders, antimicrobial and anti-inflammatory. The chloroform extract of M. sylvestris flowers and aqueous extract of S. lavandulifolia flowers were used to evaluate the wound healing activity at 200 mg/kg/day dose. Wounds were induced in Wistar rats divided into four groups as following; Group-I was treated with cold cream. Groups-II and -III were treated with cold cream containing of extracts, Group-IV received the standard drug (nitrofurazone). The efficacy of treatment was evaluated based on wound area and histopathological characteristics. The extract-treated animals by M. sylvestris showed significant reduction in the wound area when compared with other groups. Also, histopathological studies of the tissue obtained on days 6 th , th and 16 th from the extract-treated by M. sylvestris showed increased well organized bands of collagen, more fibroblasts and few inflammatory cells.
-
Evaluation of Burn Healing Properties of Arnebia euchroma and Malva sylvestris
Electronic Journal of Biology, 2009Co-Authors: Abdollah Ghasemi Pirbalouti, Mehdi Yousefi, Heshmatollah Nazari, Iraj Karimi, Abed KoohpayehAbstract:Arnebia euchroma Rolye (Johnst) (Boraginaceae) and Malva sylvestris L. (Malvaceae) are traditionally used to treat various skin disorders, and for antimicrobial, and anti-inflammatory activities. The goat lipid containing roots of Arnebia euchroma is widely used as a remedy for burn wounds in nomadic tribal (Bakhtyari) southwest Iran. We examined the effectiveness of diethyl ether extracts of Arnebia euchroma roots and Malva sylvestris flowers at 200 mg/kg/day dose. Burns were induced in Wistar rats divided into six groups as following; Group-I was treated with cold cream. Groups-II and -III were treated with cold cream containing of two extracts, Group-IV was treated with goat lipid containing of Arnebia euchroma extract (according ethnobotany survey). Group-V was treated with goat lipid. Group-VI received the standard drug (silver sulphadiazine cream). The efficacy of treatment was evaluated based on reduction of burn wound area and histopathological characteristics. The extracts-treated animals showed significant reduction (P < 0.05) in the wound area when compared with other groups. Also, histological studies of the tissue obtained on days 6, 9 and 16 from the extract-treated by goat lipid containing of Arnebia euchroma and Malva sylvestris showed increased well organized bands of collagen, more fibroblasts, and few inflammatory cells. So we concluded that Arnebia euchroma and Malva sylvestris in the form of ointment had a good potential for acceleration of burn wound healing in rats.
Abed Koohpyeh - One of the best experts on this subject based on the ideXlab platform.
-
Wound Healing Activity of Extracts of Malva sylvestris and Stachys lavandulifolia
International Journal of Biology, 2010Co-Authors: Abdollah Ghasemi Pirbalouti, Abed KoohpyehAbstract:The flowers of Stachys lavandulifolia Vahl (Lamiaceae) and Malva sylvestris Linn (Malvaceae) are traditionally used to treat various skin disorders, antimicrobial and anti-inflammatory. The chloroform extract of M. sylvestris flowers and aqueous extract of S. lavandulifolia flowers were used to evaluate the wound healing activity at 200 mg/kg/day dose. Wounds were induced in Wistar rats divided into four groups as following; Group-I was treated with cold cream. Groups-II and -III were treated with cold cream containing of extracts, Group-IV received the standard drug (nitrofurazone). The efficacy of treatment was evaluated based on wound area and histopathological characteristics. The extract-treated animals by M. sylvestris showed significant reduction in the wound area when compared with other groups. Also, histopathological studies of the tissue obtained on days 6 th , th and 16 th from the extract-treated by M. sylvestris showed increased well organized bands of collagen, more fibroblasts and few inflammatory cells.
Pedro Luiz Rosalen - One of the best experts on this subject based on the ideXlab platform.
-
Malva sylvestris derivatives as inhibitors of HIV-1 BaL infection
Natural product research, 2019Co-Authors: Bruna Benso, Pedro Luiz Rosalen, Silvana Pasetto, Maria Carolina Salomé Marquezin, Verônica Santana De Freitas-blanco, Ramiro Mendonça MurataAbstract:The emphasis of the present study is to evaluate a natural product and the potential microbicide activity using a dual chamber infection method. Malva sylvestris extracts and fractions were screene...
-
Anti-Inflammatory, Anti-Osteoclastogenic and Antioxidant Effects of Malva sylvestris Extract and Fractions: In Vitro and In Vivo Studies.
PloS one, 2016Co-Authors: Bruna Benso, Severino Matias De Alencar, Marcelo Franchin, Adna Prado Massarioli, Jonas Augusto Rizzato Paschoal, Gilson Cesar Nobre Franco, Pedro Luiz RosalenAbstract:Given their medical importance, natural products represent a tremendous source of drug discovery. The aim of this study was to investigate Malva sylvestris L. extract and fractions and their pharmacological activities followed by chemical identification. The aqueous fraction (AF) was identified as the bioactive fraction in the in vitro and in vivo assays. The AF controlled the neutrophil migration to the peritoneal cavity by 66%, inhibited the antiedematogenic activity by 58.8%, and controlled IL-1β cytokine expression by 54%. The in vitro viability tests showed a concentration-dependent effect, where the MSE and fractions at concentrations under 10 μg/mL were non-toxic to cells. Transcriptional factors of carbonic anhydrase II (CAII), cathepsin K (Ctsk) and tartrate-resistant acid phosphatase (TRAP) were analyzed by qPCR in RAW 264.7 cell lines. The gene expression analysis showed that the AF was the only treatment that could downregulate all the study genes: CAII, Ctsk and TRAP (p
-
Malva sylvestris inhibits inflammatory response in oral human cells. An in vitro infection model
PloS one, 2015Co-Authors: Bruna Benso, Pedro Luiz Rosalen, Severino Matias De Alencar, Ramiro Mendonça MurataAbstract:The aim of this study was to investigate the in vitro anti-inflammatory activity of Malva sylvestris extract (MSE) and fractions in a co-culture model of cells infected by Aggregatibacter actinomycetemcomitans. In addition, we evaluated the phytochemical content in the extract and fractions of M. sylvestris and demonstrated that polyphenols were the most frequent group in all samples studied. An in vitro dual-chamber model to mimic the periodontal structure was developed using a monolayer of epithelial keratinocytes (OBA-9) and a subepithelial layer of fibroblasts (HGF-1). The invasive periodontopathogen A. actinomycetemcomitans (D7S-1) was applied to migrate through the cell layers and induce the synthesis of immune factors and cytokines in the host cells. In an attempt to analyze the antimicrobial properties of MSE and fractions, a susceptibility test was carried out. The extract (MIC 175 μg/mL, MBC 500μg/mL) and chloroform fraction (MIC 150 μg/mL, MBC 250 μg/mL) were found to have inhibitory activity. The extract and all fractions were assessed using a cytotoxicity test and results showed that concentrations under 100 μg/mL did not significantly reduce cell viability compared to the control group (p > 0.05, viability > 90%). In order to analyze the inflammatory response, transcriptional factors and cytokines were quantified in the supernatant released from the cells. The chloroform fraction was the most effective in reducing the bacterial colonization (p< 0.05) and controlling inflammatory mediators, and promoted the down-regulation of genes including IL-1beta, IL-6, IL-10, CD14, PTGS, MMP-1 and FOS as well as the reduction of the IL-1beta, IL-6, IL-8 and GM-CSF protein levels (p< 0.05). Malva sylvestris and its chloroform fraction minimized the A. actinomycetemcomitans infection and inflammation processes in oral human cells by a putative pathway that involves important cytokines and receptors. Therefore, this natural product may be considered as a successful dual anti-inflammatory–antimicrobial candidate.
Bruna Benso - One of the best experts on this subject based on the ideXlab platform.
-
Malva sylvestris derivatives as inhibitors of HIV-1 BaL infection
Natural product research, 2019Co-Authors: Bruna Benso, Pedro Luiz Rosalen, Silvana Pasetto, Maria Carolina Salomé Marquezin, Verônica Santana De Freitas-blanco, Ramiro Mendonça MurataAbstract:The emphasis of the present study is to evaluate a natural product and the potential microbicide activity using a dual chamber infection method. Malva sylvestris extracts and fractions were screene...
-
Anti-Inflammatory, Anti-Osteoclastogenic and Antioxidant Effects of Malva sylvestris Extract and Fractions: In Vitro and In Vivo Studies.
PloS one, 2016Co-Authors: Bruna Benso, Severino Matias De Alencar, Marcelo Franchin, Adna Prado Massarioli, Jonas Augusto Rizzato Paschoal, Gilson Cesar Nobre Franco, Pedro Luiz RosalenAbstract:Given their medical importance, natural products represent a tremendous source of drug discovery. The aim of this study was to investigate Malva sylvestris L. extract and fractions and their pharmacological activities followed by chemical identification. The aqueous fraction (AF) was identified as the bioactive fraction in the in vitro and in vivo assays. The AF controlled the neutrophil migration to the peritoneal cavity by 66%, inhibited the antiedematogenic activity by 58.8%, and controlled IL-1β cytokine expression by 54%. The in vitro viability tests showed a concentration-dependent effect, where the MSE and fractions at concentrations under 10 μg/mL were non-toxic to cells. Transcriptional factors of carbonic anhydrase II (CAII), cathepsin K (Ctsk) and tartrate-resistant acid phosphatase (TRAP) were analyzed by qPCR in RAW 264.7 cell lines. The gene expression analysis showed that the AF was the only treatment that could downregulate all the study genes: CAII, Ctsk and TRAP (p
-
Malva sylvestris inhibits inflammatory response in oral human cells. An in vitro infection model
PloS one, 2015Co-Authors: Bruna Benso, Pedro Luiz Rosalen, Severino Matias De Alencar, Ramiro Mendonça MurataAbstract:The aim of this study was to investigate the in vitro anti-inflammatory activity of Malva sylvestris extract (MSE) and fractions in a co-culture model of cells infected by Aggregatibacter actinomycetemcomitans. In addition, we evaluated the phytochemical content in the extract and fractions of M. sylvestris and demonstrated that polyphenols were the most frequent group in all samples studied. An in vitro dual-chamber model to mimic the periodontal structure was developed using a monolayer of epithelial keratinocytes (OBA-9) and a subepithelial layer of fibroblasts (HGF-1). The invasive periodontopathogen A. actinomycetemcomitans (D7S-1) was applied to migrate through the cell layers and induce the synthesis of immune factors and cytokines in the host cells. In an attempt to analyze the antimicrobial properties of MSE and fractions, a susceptibility test was carried out. The extract (MIC 175 μg/mL, MBC 500μg/mL) and chloroform fraction (MIC 150 μg/mL, MBC 250 μg/mL) were found to have inhibitory activity. The extract and all fractions were assessed using a cytotoxicity test and results showed that concentrations under 100 μg/mL did not significantly reduce cell viability compared to the control group (p > 0.05, viability > 90%). In order to analyze the inflammatory response, transcriptional factors and cytokines were quantified in the supernatant released from the cells. The chloroform fraction was the most effective in reducing the bacterial colonization (p< 0.05) and controlling inflammatory mediators, and promoted the down-regulation of genes including IL-1beta, IL-6, IL-10, CD14, PTGS, MMP-1 and FOS as well as the reduction of the IL-1beta, IL-6, IL-8 and GM-CSF protein levels (p< 0.05). Malva sylvestris and its chloroform fraction minimized the A. actinomycetemcomitans infection and inflammation processes in oral human cells by a putative pathway that involves important cytokines and receptors. Therefore, this natural product may be considered as a successful dual anti-inflammatory–antimicrobial candidate.