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Kristina Wahala - One of the best experts on this subject based on the ideXlab platform.

  • optimization of a liquid chromatography tandem mass spectrometry method for quantification of the plant lignans secoisolariciresinol Matairesinol lariciresinol and pinoresinol in foods
    Journal of Agricultural and Food Chemistry, 2004
    Co-Authors: I E J Milder, Johan J P Lasaroms, Kristina Wahala, Dini Venema, Ilja C W Arts, Peter C H Hollman
    Abstract:

    A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of the four major enterolignan precursors [secoisolariciresinol, Matairesinol, lariciresinol, and pinoresinol] in foods. The method consists of alkaline methanolic extraction, followed by enzymatic hydrolysis using Helix pomatia (H. pomatia) -glucuronidase/sulfatase. H. pomatia was selected from several enzymes based on its ability to hydrolyze isolated lignan glucosides. After ether extraction samples were analyzed and quantified against secoisolariciresinol-d8 and Matairesinol-d6. The method was optimized using model products: broccoli, bread, flaxseed, and tea. The yield of methanolic extraction increased up to 81°when it was combined with alkaline hydrolysis. Detection limits were 4-10 g/(100 g dry weight) for solid foods and 0.2-0.4 g/(100 mL) for beverages. Within- and between-run coefficients of variation were 6-21 and 6-33°respectively. Recovery of lignans added to model products was satisfactory (73-123) except for Matairesinol added to bread (51-55)

  • optimization of a liquid chromatography tandem mass spectrometry method for quantification of the plant lignans secoisolariciresinol Matairesinol lariciresinol and pinoresinol in foods
    Journal of Agricultural and Food Chemistry, 2004
    Co-Authors: I E J Milder, Johan J P Lasaroms, Kristina Wahala, Dini Venema, Ilja C W Arts, Peter C H Hollman
    Abstract:

    A liquid chromatography−tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of the four major enterolignan precursors [secoisolariciresinol, Matairesinol, lariciresinol,...

  • in vitro metabolism of plant lignans new precursors of mammalian lignans enterolactone and enterodiol
    Journal of Agricultural and Food Chemistry, 2001
    Co-Authors: Satu-maarit Heinonen, Kristina Wahala, Kirsi Liukkonen, Takeshi Deyama, Sansei Nishibe, Tarja Nurmi, Herman Adlercreutz
    Abstract:

    The metabolism of the plant lignans Matairesinol, secoisolariciresinol, pinoresinol, syringaresinol, arctigenin, 7-hydroxyMatairesinol, isolariciresinol, and lariciresinol by human fecal microflora was investigated to study their properties as mammalian lignan precursors. The quantitative analyses of lignan precursors and the mammalian lignans enterolactone and enterodiol were performed by HPLC with coulometric electrode array detector. The metabolic products, including mammalian lignans, were characterized as trimethylsilyl derivatives by gas chromatography−mass spectrometry. Matairesinol, secoisolariciresinol, lariciresinol, and pinoresinol were converted to mammalian lignans only. Several metabolites were isolated and tentatively identified as for syringaresinol and arctigenin in addition to the mammalian lignans. Metabolites of 7-hydroxyMatairesinol were characterized as enterolactone and 7-hydroxyenterolactone by comparison with authentic reference compounds. A metabolic scheme describing the convers...

  • in vitro metabolism of plant lignans new precursors of mammalian lignans enterolactone and enterodiol
    Journal of Agricultural and Food Chemistry, 2001
    Co-Authors: Satu Heinone, Kristina Wahala, Takeshi Deyama, Sansei Nishibe, Tarja Nurmi, Kirsi Liukkone, Kaisa Poutane, Herman Adlercreutz
    Abstract:

    The metabolism of the plant lignans Matairesinol, secoisolariciresinol, pinoresinol, syringaresinol, arctigenin, 7-hydroxyMatairesinol, isolariciresinol, and lariciresinol by human fecal microflora was investigated to study their properties as mammalian lignan precursors. The quantitative analyses of lignan precursors and the mammalian lignans enterolactone and enterodiol were performed by HPLC with coulometric electrode array detector. The metabolic products, including mammalian lignans, were characterized as trimethylsilyl derivatives by gas chromatography-mass spectrometry. Matairesinol, secoisolariciresinol, lariciresinol, and pinoresinol were converted to mammalian lignans only. Several metabolites were isolated and tentatively identified as for syringaresinol and arctigenin in addition to the mammalian lignans. Metabolites of 7-hydroxyMatairesinol were characterized as enterolactone and 7-hydroxyenterolactone by comparison with authentic reference compounds. A metabolic scheme describing the conversion of the most abundant new mammalian lignan precursors, pinoresinol and lariciresinol, is presented.

  • isotope dilution gas chromatographic mass spectrometric method for the determination of unconjugated lignans and isoflavonoids in human feces with preliminary results in omnivorous and vegetarian women
    Analytical Biochemistry, 1995
    Co-Authors: Herman Adlercreutz, Kristina Wahala, Theodore Fotsis, Mindy S Kurzer, Taru Makela, Tapio Hase
    Abstract:

    We describe an isotope dilution gas chromatographic-mass spectrometric (GC/MS) method for the identification and quantitative determination of the lignans enterolactone, enterodiol, and Matairesinol and the isoflavonoids daidzein, equol, O-desmethylangolensin, and genistein in feces. Following the addition of deuterated internal standards for all compounds, the feces samples are extracted and purified in several ion exchange chromatographic steps. Following formation of trimethylsilyl ethers, the samples are analyzed by combined capillary column GC/MS in the selective ion monitoring mode and corrected for all losses during the procedure using the deuterated internal standards. Results on the reliability of the method and values for nine Finnish omnivorous and nine vegetarian women are presented.

Peter C H Hollman - One of the best experts on this subject based on the ideXlab platform.

  • intake of the plant lignans secoisolariciresinol Matairesinol lariciresinol and pinoresinol in dutch men and women
    Journal of Nutrition, 2005
    Co-Authors: I E J Milder, Bas Bueno H De Mesquita, Peter C H Hollman, Ilja C W Arts, Edith J M Feskens, Daan Kromhout
    Abstract:

    Enterolignans (enterolactone and enterodiol) are phytoestrogens that are formed by the colonic microflora from plant lignans. They may reduce the risk of certain types of cancer and cardiovascular diseases. Initially, only secoisolariciresinol and Matairesinol were considered to be enterolignan precursors, but recently, new precursors such as lariciresinol and pinoresinol were identified. We recently developed a lignan database including 4 major enterolignan precursors. We used this database to estimate lignan intake in a representative sample of Dutch men and women participating in the Dutch Food Consumption Survey, carried out in 1997?1998. Median total lignan intake among 4660 adults (19?97 y old) was 979 µg/d. Total lignan intake did not differ between men and women; thus, the lignan density of the diet was significantly higher (P <0.001) in women than in men. Lignan intake was strongly skewed toward higher values (range 43?77584 µg/d, mean 1241 µg/d). Lariciresinol and pinoresinol contributed 75% to lignan intake, whereas secoisolariciresinol and Matairesinol contributed only 25%. The major food sources of lignans were beverages (37%), vegetables (24%), nuts and seeds (14%), bread (9%), and fruits (7%). Lignan intake was significantly (P <0.001) correlated with intake of dietary fiber (r = 0.46), folate (r = 0.39), and vitamin C (r = 0.44). Older persons, nonsmokers, vegetarians, and persons with a low BMI or a high socioeconomic status had higher lignan intakes than their counterparts. In brief, this study shows that the amount of enterolignan precursors in the diet has previously been largely underestimated

  • Intake of the Plant Lignans Secoisolariciresinol, Matairesinol, Lariciresinol, and Pinoresinol in Dutch Men and Women
    Journal of Nutrition, 2005
    Co-Authors: I E J Milder, H. Bas Bueno De Mesquita, Peter C H Hollman, Ilja C W Arts, Edith J M Feskens, Daan Kromhout
    Abstract:

    Enterolignans (enterolactone and enterodiol) are phytoestrogens that are formed by the colonic microflora from plant lignans. They may reduce the risk of certain types of cancer and cardiovascular diseases. Initially, only secoisolariciresinol and Matairesinol were considered to be enterolignan precursors, but recently, new precursors such as lariciresinol and pinoresinol were identified. We recently developed a lignan database including 4 major enterolignan precursors. We used this database to estimate lignan intake in a representative sample of Dutch men and women participating in the Dutch Food Consumption Survey, carried out in 1997?1998. Median total lignan intake among 4660 adults (19?97 y old) was 979 µg/d. Total lignan intake did not differ between men and women; thus, the lignan density of the diet was significantly higher (P

  • lignan contents of dutch plant foods a database including lariciresinol pinoresinol secoisolariciresinol and Matairesinol
    British Journal of Nutrition, 2005
    Co-Authors: I E J Milder, Betty Van De Putte, Dini Venema, Ilja C W Arts, Peter C H Hollman
    Abstract:

    Enterolignans ( enterodiol and enterolactone) can potentially reduce the risk of certain cancers and cardiovascular diseases. Enterolignans are formed by the intestinal microflora after the consumption of plant lignans. Until recently, only secoisolariciresinol and Matairesinol were considered enterolignan precursors, but now several new precursors have been identified, of which lariciresinol and pinoresinol have a high degree of conversion. Quantitative data on the contents in foods of these new enterolignan precursors are not available. Thus, the aim of this study was to compile a lignan database including all four major enterolignan precursors. Liquid chromatography-tandem mass spectrometry was used to quantify lariciresinol, pinoresinol, secoisolariciresinol and Matairesinol in eighty-three solid foods and twenty-six beverages commonly consumed in The Netherlands. The richest source of lignans was flaxseed (301 129 μ g/100 g), which contained mainly secoisolariciresinol. Also, lignan concentrations in sesame seeds (29 331 μ g/100 g, mainly pinoresinol and lariciresinol) were relatively high. For grain products, which are known to be important sources of lignan, lignan concentrations ranged from 7 to 764 μ g/100 g. However, many vegetables and fruits had similar concentrations, because of the contribution of lariciresinol and pinoresinol. Brassica vegetables contained unexpectedly high levels of lignans (185-2321 μ g/100 g), mainly pinoresinol and lariciresinol. Lignan levels in beverages varied from 0 (cola) to 91 μ g/100 ml (red wine). Only four of the 109 foods did not contain a measurable amount of lignans, and in most cases the amount of lariciresinol and pinoresinol was larger than that of secoisolariciresinol and Matairesinol. Thus, available databases largely underestimate the amount of enterolignan precursors in foods.

  • optimization of a liquid chromatography tandem mass spectrometry method for quantification of the plant lignans secoisolariciresinol Matairesinol lariciresinol and pinoresinol in foods
    Journal of Agricultural and Food Chemistry, 2004
    Co-Authors: I E J Milder, Johan J P Lasaroms, Kristina Wahala, Dini Venema, Ilja C W Arts, Peter C H Hollman
    Abstract:

    A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of the four major enterolignan precursors [secoisolariciresinol, Matairesinol, lariciresinol, and pinoresinol] in foods. The method consists of alkaline methanolic extraction, followed by enzymatic hydrolysis using Helix pomatia (H. pomatia) -glucuronidase/sulfatase. H. pomatia was selected from several enzymes based on its ability to hydrolyze isolated lignan glucosides. After ether extraction samples were analyzed and quantified against secoisolariciresinol-d8 and Matairesinol-d6. The method was optimized using model products: broccoli, bread, flaxseed, and tea. The yield of methanolic extraction increased up to 81°when it was combined with alkaline hydrolysis. Detection limits were 4-10 g/(100 g dry weight) for solid foods and 0.2-0.4 g/(100 mL) for beverages. Within- and between-run coefficients of variation were 6-21 and 6-33°respectively. Recovery of lignans added to model products was satisfactory (73-123) except for Matairesinol added to bread (51-55)

  • optimization of a liquid chromatography tandem mass spectrometry method for quantification of the plant lignans secoisolariciresinol Matairesinol lariciresinol and pinoresinol in foods
    Journal of Agricultural and Food Chemistry, 2004
    Co-Authors: I E J Milder, Johan J P Lasaroms, Kristina Wahala, Dini Venema, Ilja C W Arts, Peter C H Hollman
    Abstract:

    A liquid chromatography−tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of the four major enterolignan precursors [secoisolariciresinol, Matairesinol, lariciresinol,...

Herman Adlercreutz - One of the best experts on this subject based on the ideXlab platform.

  • quantification of lignans in food using isotope dilution gas chromatography mass spectrometry
    Journal of Agricultural and Food Chemistry, 2005
    Co-Authors: Jose L Penalvo, Kati Haajanen, Nigel P Botting, Herman Adlercreutz
    Abstract:

    The optimization and validation of a protocol for the quantification of six dietary lignans, i.e., secoisolariciresinol, Matairesinol, lariciresinol, pinoresinol, medioresinol, and syringaresinol, in food are presented. The method incorporates isotope dilution to ensure correct accuracy and precision, introducing the utilization of individual stable 13 C3-labeled lignans. To demonstrate the potential of this new method, preliminary results of the levels of dietary lignans in selected foods are presented. It is concluded that the method fulfils the reliability criteria and can be applied to the analysis of the most common lignans in human food, being an essential asset to establish the intake of lignans in a determined population and their relation with disease prevention.

  • Rye Bread in the Diet of Pigs Enhances the Formation of Enterolactone and Increases Its Levels in Plasma, Urine and Feces
    Journal of Nutrition, 2003
    Co-Authors: Knud Erik Bach Knudsen, Anna Kirstin Bjørnbak Kjær, Inge Tetens, Tarja Nurmi, Satu-maarit Heinonen, Anja Serena, Herman Adlercreutz
    Abstract:

    To obtain new insight into the quantitative and qualitative metabolism of rye and wheat lignans, we performed three series of experiments with catheterized pigs. Two diets with similar levels of dietary fiber and macronutrients but with contrasting levels of plant lignans (isolariciresinol, lariciresinol, Matairesinol, pinoresinol, secoisolariciresinol and syringaresinol) were prepared from rye (high in lignans) and wheat (low in lignans) soft and crisp breads. In two series of experiments we quantified the uptake from the gut of enterolactone in four pigs fitted with catheters in the portal vein and mesenteric artery and with an ultrasonic flow probe attached to the portal vein to monitor the blood flow. In a third study with six pigs, we quantified the bioavailability of the plant lignans that can be converted to enterolactone (lariciresinol, Matairesinol, pinoresinol, secoisolariciresinol and syringaresinol) and the concentration in the peripheral blood. Plant and mammalian lignans in diets and stool were analyzed by isotope dilution gas chromatography-mass spectrometry and enterolactone in plasma and urine determined by time-resolved fluoroimmunoassay. There was a significantly higher formation of enterolactone in pigs fed the rye diet, and higher fecal and urinary excretion and circulating levels of mammalian lignans than in pigs fed the wheat diet. The conversion of mammalian lignan precursors to enterolactone was 48% with the wheat diet and 60% with the rye diet. Mammalian lignans are absorbed by passive diffusion from the large intestine and a substantial fraction of the absorbed mammalian lignans undergoes enterohepatic circulation, resulting in low diurnal variation in plasma levels of enterolactone.

  • in vitro metabolism of plant lignans new precursors of mammalian lignans enterolactone and enterodiol
    Journal of Agricultural and Food Chemistry, 2001
    Co-Authors: Satu-maarit Heinonen, Kristina Wahala, Kirsi Liukkonen, Takeshi Deyama, Sansei Nishibe, Tarja Nurmi, Herman Adlercreutz
    Abstract:

    The metabolism of the plant lignans Matairesinol, secoisolariciresinol, pinoresinol, syringaresinol, arctigenin, 7-hydroxyMatairesinol, isolariciresinol, and lariciresinol by human fecal microflora was investigated to study their properties as mammalian lignan precursors. The quantitative analyses of lignan precursors and the mammalian lignans enterolactone and enterodiol were performed by HPLC with coulometric electrode array detector. The metabolic products, including mammalian lignans, were characterized as trimethylsilyl derivatives by gas chromatography−mass spectrometry. Matairesinol, secoisolariciresinol, lariciresinol, and pinoresinol were converted to mammalian lignans only. Several metabolites were isolated and tentatively identified as for syringaresinol and arctigenin in addition to the mammalian lignans. Metabolites of 7-hydroxyMatairesinol were characterized as enterolactone and 7-hydroxyenterolactone by comparison with authentic reference compounds. A metabolic scheme describing the convers...

  • in vitro metabolism of plant lignans new precursors of mammalian lignans enterolactone and enterodiol
    Journal of Agricultural and Food Chemistry, 2001
    Co-Authors: Satu Heinone, Kristina Wahala, Takeshi Deyama, Sansei Nishibe, Tarja Nurmi, Kirsi Liukkone, Kaisa Poutane, Herman Adlercreutz
    Abstract:

    The metabolism of the plant lignans Matairesinol, secoisolariciresinol, pinoresinol, syringaresinol, arctigenin, 7-hydroxyMatairesinol, isolariciresinol, and lariciresinol by human fecal microflora was investigated to study their properties as mammalian lignan precursors. The quantitative analyses of lignan precursors and the mammalian lignans enterolactone and enterodiol were performed by HPLC with coulometric electrode array detector. The metabolic products, including mammalian lignans, were characterized as trimethylsilyl derivatives by gas chromatography-mass spectrometry. Matairesinol, secoisolariciresinol, lariciresinol, and pinoresinol were converted to mammalian lignans only. Several metabolites were isolated and tentatively identified as for syringaresinol and arctigenin in addition to the mammalian lignans. Metabolites of 7-hydroxyMatairesinol were characterized as enterolactone and 7-hydroxyenterolactone by comparison with authentic reference compounds. A metabolic scheme describing the conversion of the most abundant new mammalian lignan precursors, pinoresinol and lariciresinol, is presented.

  • isotope dilution gas chromatographic mass spectrometric method for the determination of unconjugated lignans and isoflavonoids in human feces with preliminary results in omnivorous and vegetarian women
    Analytical Biochemistry, 1995
    Co-Authors: Herman Adlercreutz, Kristina Wahala, Theodore Fotsis, Mindy S Kurzer, Taru Makela, Tapio Hase
    Abstract:

    We describe an isotope dilution gas chromatographic-mass spectrometric (GC/MS) method for the identification and quantitative determination of the lignans enterolactone, enterodiol, and Matairesinol and the isoflavonoids daidzein, equol, O-desmethylangolensin, and genistein in feces. Following the addition of deuterated internal standards for all compounds, the feces samples are extracted and purified in several ion exchange chromatographic steps. Following formation of trimethylsilyl ethers, the samples are analyzed by combined capillary column GC/MS in the selective ion monitoring mode and corrected for all losses during the procedure using the deuterated internal standards. Results on the reliability of the method and values for nine Finnish omnivorous and nine vegetarian women are presented.

I E J Milder - One of the best experts on this subject based on the ideXlab platform.

  • intake of the plant lignans secoisolariciresinol Matairesinol lariciresinol and pinoresinol in dutch men and women
    Journal of Nutrition, 2005
    Co-Authors: I E J Milder, Bas Bueno H De Mesquita, Peter C H Hollman, Ilja C W Arts, Edith J M Feskens, Daan Kromhout
    Abstract:

    Enterolignans (enterolactone and enterodiol) are phytoestrogens that are formed by the colonic microflora from plant lignans. They may reduce the risk of certain types of cancer and cardiovascular diseases. Initially, only secoisolariciresinol and Matairesinol were considered to be enterolignan precursors, but recently, new precursors such as lariciresinol and pinoresinol were identified. We recently developed a lignan database including 4 major enterolignan precursors. We used this database to estimate lignan intake in a representative sample of Dutch men and women participating in the Dutch Food Consumption Survey, carried out in 1997?1998. Median total lignan intake among 4660 adults (19?97 y old) was 979 µg/d. Total lignan intake did not differ between men and women; thus, the lignan density of the diet was significantly higher (P <0.001) in women than in men. Lignan intake was strongly skewed toward higher values (range 43?77584 µg/d, mean 1241 µg/d). Lariciresinol and pinoresinol contributed 75% to lignan intake, whereas secoisolariciresinol and Matairesinol contributed only 25%. The major food sources of lignans were beverages (37%), vegetables (24%), nuts and seeds (14%), bread (9%), and fruits (7%). Lignan intake was significantly (P <0.001) correlated with intake of dietary fiber (r = 0.46), folate (r = 0.39), and vitamin C (r = 0.44). Older persons, nonsmokers, vegetarians, and persons with a low BMI or a high socioeconomic status had higher lignan intakes than their counterparts. In brief, this study shows that the amount of enterolignan precursors in the diet has previously been largely underestimated

  • Intake of the Plant Lignans Secoisolariciresinol, Matairesinol, Lariciresinol, and Pinoresinol in Dutch Men and Women
    Journal of Nutrition, 2005
    Co-Authors: I E J Milder, H. Bas Bueno De Mesquita, Peter C H Hollman, Ilja C W Arts, Edith J M Feskens, Daan Kromhout
    Abstract:

    Enterolignans (enterolactone and enterodiol) are phytoestrogens that are formed by the colonic microflora from plant lignans. They may reduce the risk of certain types of cancer and cardiovascular diseases. Initially, only secoisolariciresinol and Matairesinol were considered to be enterolignan precursors, but recently, new precursors such as lariciresinol and pinoresinol were identified. We recently developed a lignan database including 4 major enterolignan precursors. We used this database to estimate lignan intake in a representative sample of Dutch men and women participating in the Dutch Food Consumption Survey, carried out in 1997?1998. Median total lignan intake among 4660 adults (19?97 y old) was 979 µg/d. Total lignan intake did not differ between men and women; thus, the lignan density of the diet was significantly higher (P

  • lignan contents of dutch plant foods a database including lariciresinol pinoresinol secoisolariciresinol and Matairesinol
    British Journal of Nutrition, 2005
    Co-Authors: I E J Milder, Betty Van De Putte, Dini Venema, Ilja C W Arts, Peter C H Hollman
    Abstract:

    Enterolignans ( enterodiol and enterolactone) can potentially reduce the risk of certain cancers and cardiovascular diseases. Enterolignans are formed by the intestinal microflora after the consumption of plant lignans. Until recently, only secoisolariciresinol and Matairesinol were considered enterolignan precursors, but now several new precursors have been identified, of which lariciresinol and pinoresinol have a high degree of conversion. Quantitative data on the contents in foods of these new enterolignan precursors are not available. Thus, the aim of this study was to compile a lignan database including all four major enterolignan precursors. Liquid chromatography-tandem mass spectrometry was used to quantify lariciresinol, pinoresinol, secoisolariciresinol and Matairesinol in eighty-three solid foods and twenty-six beverages commonly consumed in The Netherlands. The richest source of lignans was flaxseed (301 129 μ g/100 g), which contained mainly secoisolariciresinol. Also, lignan concentrations in sesame seeds (29 331 μ g/100 g, mainly pinoresinol and lariciresinol) were relatively high. For grain products, which are known to be important sources of lignan, lignan concentrations ranged from 7 to 764 μ g/100 g. However, many vegetables and fruits had similar concentrations, because of the contribution of lariciresinol and pinoresinol. Brassica vegetables contained unexpectedly high levels of lignans (185-2321 μ g/100 g), mainly pinoresinol and lariciresinol. Lignan levels in beverages varied from 0 (cola) to 91 μ g/100 ml (red wine). Only four of the 109 foods did not contain a measurable amount of lignans, and in most cases the amount of lariciresinol and pinoresinol was larger than that of secoisolariciresinol and Matairesinol. Thus, available databases largely underestimate the amount of enterolignan precursors in foods.

  • optimization of a liquid chromatography tandem mass spectrometry method for quantification of the plant lignans secoisolariciresinol Matairesinol lariciresinol and pinoresinol in foods
    Journal of Agricultural and Food Chemistry, 2004
    Co-Authors: I E J Milder, Johan J P Lasaroms, Kristina Wahala, Dini Venema, Ilja C W Arts, Peter C H Hollman
    Abstract:

    A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of the four major enterolignan precursors [secoisolariciresinol, Matairesinol, lariciresinol, and pinoresinol] in foods. The method consists of alkaline methanolic extraction, followed by enzymatic hydrolysis using Helix pomatia (H. pomatia) -glucuronidase/sulfatase. H. pomatia was selected from several enzymes based on its ability to hydrolyze isolated lignan glucosides. After ether extraction samples were analyzed and quantified against secoisolariciresinol-d8 and Matairesinol-d6. The method was optimized using model products: broccoli, bread, flaxseed, and tea. The yield of methanolic extraction increased up to 81°when it was combined with alkaline hydrolysis. Detection limits were 4-10 g/(100 g dry weight) for solid foods and 0.2-0.4 g/(100 mL) for beverages. Within- and between-run coefficients of variation were 6-21 and 6-33°respectively. Recovery of lignans added to model products was satisfactory (73-123) except for Matairesinol added to bread (51-55)

  • optimization of a liquid chromatography tandem mass spectrometry method for quantification of the plant lignans secoisolariciresinol Matairesinol lariciresinol and pinoresinol in foods
    Journal of Agricultural and Food Chemistry, 2004
    Co-Authors: I E J Milder, Johan J P Lasaroms, Kristina Wahala, Dini Venema, Ilja C W Arts, Peter C H Hollman
    Abstract:

    A liquid chromatography−tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of the four major enterolignan precursors [secoisolariciresinol, Matairesinol, lariciresinol,...

Annika Smeds - One of the best experts on this subject based on the ideXlab platform.

  • Novel Lignan and Stilbenoid Mixture Shows Anticarcinogenic Efficacy in Preclinical PC-3M-luc2 Prostate Cancer Model
    2016
    Co-Authors: Emrah Yatkin, Annika Smeds, Bjarne Holmbom, Lauri Polari, Teemu D Laajala, Christer Eckerman, Tero Aittokallio, Niina M. Saarinen, Sari I. Mäkelä
    Abstract:

    Prostate cancer is the most common cancer of men in the Western world, and novel approaches for prostate cancer risk reduction are needed. Plant-derived phenolic compounds attenuate prostate cancer growth in preclinical models by several mechanisms, which is in line with epidemiological findings suggesting that consumption of plant-based diets is associated with low risk of prostate cancer. The objective of this study was to assess the effects of a novel lignan-stilbenoid mixture in PC-3M-luc2 human prostate cancer cells in vitro and in orthotopic xenografts. Lignan and stilbenoid –rich extract was obtained from Scots pine (Pinus sylvestris) knots. Pine knot extract as well as stilbenoids (methyl pinosylvin and pinosylvin), and lignans (Matairesinol and nortrachelogenin) present in pine knot extract showed antiproliferative and proapoptotic efficacy at $40 mM concentration in vitro. Furthermore, pine knot extract derived stilbenoids enhanced tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induced apoptosis already at $10 mM concentrations. In orthotopic PC-3M-luc2 xenograft bearing immunocompromized mice, three-week peroral exposure to pine knot extract (52 mg of lignans and stilbenoids per kg of body weight) was well tolerated and showed anti-tumorigenic efficacy, demonstrated by multivariate analysis combining essential markers of tumor growth (i.e. tumor volume, vascularization, and cell proliferation). Methyl pinosylvin, pinosylvin, Matairesinol, nortrachelogenin, as well as resveratrol, a metabolite of pinosylvin, were detected in serum at total concentration of 7273 mM, confirming the bioavailability of pine knot extract derived lignans and stilbenoids

  • novel lignan and stilbenoid mixture shows anticarcinogenic efficacy in preclinical pc 3m luc2 prostate cancer model
    PLOS ONE, 2014
    Co-Authors: Emrah Yatkin, Annika Smeds, Bjarne Holmbom, Lauri Polari, Teemu D Laajala, Christer Eckerman, Niina Saarinen, Tero Aittokallio, Sari Makela
    Abstract:

    Prostate cancer is the most common cancer of men in the Western world, and novel approaches for prostate cancer risk reduction are needed. Plant-derived phenolic compounds attenuate prostate cancer growth in preclinical models by several mechanisms, which is in line with epidemiological findings suggesting that consumption of plant-based diets is associated with low risk of prostate cancer. The objective of this study was to assess the effects of a novel lignan-stilbenoid mixture in PC-3M-luc2 human prostate cancer cells in vitro and in orthotopic xenografts. Lignan and stilbenoid ‐rich extract was obtained from Scots pine (Pinus sylvestris) knots. Pine knot extract as well as stilbenoids (methyl pinosylvin and pinosylvin), and lignans (Matairesinol and nortrachelogenin) present in pine knot extract showed antiproliferative and proapoptotic efficacy at $40 mM concentration in vitro. Furthermore, pine knot extract derived stilbenoids enhanced tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induced apoptosis already at $10 mM concentrations. In orthotopic PC-3Mluc2 xenograft bearing immunocompromized mice, three-week peroral exposure to pine knot extract (52 mg of lignans and stilbenoids per kg of body weight) was well tolerated and showed anti-tumorigenic efficacy, demonstrated by multivariate analysis combining essential markers of tumor growth (i.e. tumor volume, vascularization, and cell proliferation). Methyl pinosylvin, pinosylvin, Matairesinol, nortrachelogenin, as well as resveratrol, a metabolite of pinosylvin, were detected in serum at total concentration of 7273 mM, confirming the bioavailability of pine knot extract derived lignans and stilbenoids. In summary, our data indicates that pine knot extract is a novel and cost-effective source of resveratrol, methyl pinosylvin and other bioactive lignans and stilbenoids. Pine knot extract shows anticarcinogenic efficacy in preclinical prostate cancer model, and our in vitro data suggests that compounds derived from the extract may have potential as novel chemosensitizers to TRAIL. These findings promote further research on health-related applications of wood biochemicals.

  • Concentrations of PKE-derived phenolic compounds in serum of PC-3M-luc2 orthotopic xenograft bearing mice.a
    2014
    Co-Authors: Emrah Yatkin, Annika Smeds, Bjarne Holmbom, Lauri Polari, Teemu D Laajala, Christer Eckerman, Tero Aittokallio, Niina M. Saarinen, Sari I. Mäkelä
    Abstract:

    aTumor bearing male mice were gavaged with vehicle or PKE for 3 weeks. Terminal serum samples were collected 2–3 hours after the last gavage of PKE. NTG, nortachelogenin; MePS, pinosylvin monomethyl ether; PS, pinosylvin; HMR, 7-hydroxyMatairesinol; MR, Matairesinol; PST, pinostilbene; RV, resveratrol; EL, enterolactone. Concentrations (μM) are expressed as mean±SEM.*p

  • PKE and its compounds induce A) apoptosis and B) G0/G1 cell cycle arrest of PC-3M-luc2 cells in vitro.
    2014
    Co-Authors: Emrah Yatkin, Annika Smeds, Bjarne Holmbom, Lauri Polari, Teemu D Laajala, Christer Eckerman, Tero Aittokallio, Niina M. Saarinen, Sari I. Mäkelä
    Abstract:

    Apoptosis rates and number of cells in G0/G1 phase are expressed relative to vehicle control (Ctrl = 1). The cells were treated with test compounds for 48 hours and apoptosis rate and cell cycle phase was determined with flow cytometer. LS, mixture of main PKE lignans and stilbenes; MePS, pinosylvin monomethyl ether; NTG, nortrachelogenin; PS, pinosylvin; MR, Matairesinol; Ab, abietic acid; RV, resveratrol. The data are means of three independent experiments ± SEM. Statistical significance was determined by non-parametric t-test for each treatment in comparison to respective Ctrl. *p

  • PKE and its compounds sensitize PC-3M-luc2 cells to TRAIL -induced apoptosis in vitro.
    2014
    Co-Authors: Emrah Yatkin, Annika Smeds, Bjarne Holmbom, Lauri Polari, Teemu D Laajala, Christer Eckerman, Tero Aittokallio, Niina M. Saarinen, Sari I. Mäkelä
    Abstract:

    A) Cells treated with PKE in combination with increasing concentrations of human TRAIL (0−100 ng/ml), values are percentages of nuclear fragmentation. B) Cells treated with PKE, LS mixture, or with individual PKE compounds in combination with human TRAIL (25 ng/ml), values are relative apoptosis rates (Ctrl = 1). The cells were treated with test compounds for 48 hours and nuclear fragmentation was determined with flow cytometer. LS, mixture of main PKE lignans and stilbenes; MePS, pinosylvin monomethyl ether; NTG, nortrachelogenin; PS, pinosylvin; MR, Matairesinol; Ab, abietic acid; RV, resveratrol. The data are means of three independent experiments ± SEM. Statistical significance was determined by non-parametric t-test for each treatment in comparison to respective vehicle treatment (Ctrl). *p