The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform
Richard C. Hamelin - One of the best experts on this subject based on the ideXlab platform.
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Genome-enhanced detection and identification of fungal pathogens responsible for pine and poplar rust diseases
2019Co-Authors: Marie-josée Bergeron, Philippe Tanguay, Nicolas Feau, Don Stewart, Richard C. HamelinAbstract:Biosurveillance is a proactive approach that may help to limit the spread of invasive fungal pathogens of trees, such as rust fungi which have caused some of the world’s most damaging diseases of pines and poplars. Most of these fungi have a complex life cycle, with up to five spore stages, which is completed on two different hosts. They have a biotrophic lifestyle and may be propagated by asymptomatic plant material, complicating their detection and identification. A bioinformatics approach, based on whole genome comparison, was used to identify genome regions that are unique to the white pine blister rust fungus, Cronartium ribicola, the poplar leaf rust fungi Melampsora medusae and Melampsora larici-populina or to members of either the Cronartium and Melampsora genera. Species- and genus-specific real-time PCR assays, targeting these unique regions, were designed with the aim of detecting each of these five taxonomic groups. In total, twelve assays were developed and tested over a wide range of samples, including different spore types, different infected plant parts on the pycnio-aecial or uredinio-telial host, and captured insect vectors. One hundred percent detection accuracy was achieved for the three targeted species and two genera with either a single assay or a combination of two assays. This proof of concept experiment on pine and poplar leaf rust fungi demonstrates that the genome-enhanced detection and identification approach can be translated into effective real-time PCR assays to monitor tree fungal pathogens.
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Genus- and species-specific assays for hierarchical detection of Melampsora spp., Melampsora medusae and Melampsora larici-populina.
2019Co-Authors: Marie-josée Bergeron, Philippe Tanguay, Nicolas Feau, Don Stewart, Richard C. HamelinAbstract:Genus- and species-specific assays for hierarchical detection of Melampsora spp., Melampsora medusae and Melampsora larici-populina.
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Simultaneous monitoring and quantification of Melampsora allii‐populina and Melampsora larici‐populina on infected poplar leaves using a duplex real‐time PCR assay
Plant Pathology, 2015Co-Authors: Cécile Guinet, Anne-laure Boutigny, Agathe Vialle, Pascal Frey, Richard C. Hamelin, Renaud IoosAbstract:The rust fungi Melampsora larici-populina (Mlp) and Melampsora allii-populina (Map) are the main phytosanitary constraints for commercial poplar cultivation in Europe. Although Mlp is more aggressive and prevalent than Map, the two species may co-infect the same poplar tree or even the same poplar leaf, making the epidemiological surveys of each species difficult to achieve. In this study, a new duplex real-time PCR assay targeting each species was developed, based on single-copy genes. This test proved to be specific, inclusive and was successfully used to detect and quantify each species, starting from urediniospore samples or directly from infected poplar leaves, with or without visible symptoms. This new molecular tool was also assessed for comparative studies of time-course infection experiments on artificially inoculated poplar leaf discs. These studies showed that the growth dynamics of Map were significantly slower when the two species were co-inoculated on the same leaf disc, confirming that Map is less aggressive than Mlp.
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Optimization of a real-time PCR assay for the detection of the quarantine pathogen Melampsora medusae f. sp deltoidae
Fungal Biology, 2013Co-Authors: Anne-laure Boutigny, Claude Husson, Cécile Guinet, Agathe Vialle, Pascal Frey, Richard C. Hamelin, Axelle Andrieux, Renaud IoosAbstract:Melampsora medusae (Mm), one of the causal agents of poplar rust, is classified as an A2 quarantine pest for European Plant Protection Organization (EPPO) and its presence in Europe is strictly controlled. Two formae speciales have been described within Mm, Melampsora medusae f. sp. deltoidae (Mmd), and Melampsora medusae f. sp. tremuloidae (Mmt) on the basis of their pathogenicity on Populus species from the section Aigeiros (e.g. Populus deltoides) or Populus (e.g. Populus tremuloides), respectively. In this study, a real-time polymerase chain reaction (PCR) assay was developed allowing the detection of Mmd, the forma specialis that is economically harmful. A set of primers and hydrolysis probe were designed based on sequence polymorphisms in the large ribosomal RNA subunit (28S). The real-time PCR assay was optimized and performance criteria of the detection method, i.e. sensitivity, specificity, repeatability, reproducibility, and robustness, were assessed. The real-time PCR method was highly specific and sensitive and allowed the detection of one single urediniospore of Mmd in a mixture of 2 mg of urediniospores of other Melampsora species. This test offers improved specificity over currently existing conventional PCR tests and can be used for specific surveys in European nurseries and phytosanitary controls, in order to avoid introduction and spread of this pathogen in Europe.
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phylogenetic species recognition reveals host specific lineages among poplar rust fungi
Molecular Phylogenetics and Evolution, 2013Co-Authors: Agathe Vialle, Pascal Frey, Nicolas Feau, Louis Bernier, Richard C. HamelinAbstract:Fungal species belonging to the genus Melampsora (Basidiomycota, Pucciniales) comprise rust pathogens that alternate between Salicaceae and other plant hosts. Species delineation and identification are difficult within this group due to the paucity of observable morphological features. Several Melampsora rusts are highly host-specific and this feature has been used for identification at the species level. However, this criterion is not always reliable since different Melampsora rust species can overlap on one host but specialize on a different one. To date, two different species recognition methods are used to recognize and define species within the Melampsora genus: (i) morphological species recognition, which is based solely on morphological criteria; and (ii) ecological species recognition, which combines morphological criteria with host range to recognize and define species. In order to clarify species recognition within the Melampsora genus, we applied phylogenetic species recognition to Melampsora poplar rusts by conducting molecular phylogenetic analyses on 15 Melampsora taxa using six nuclear and mitochondrial loci. By assessing the genealogical concordance between phylogenies, we identified 12 lineages that evolved independently, corresponding to distinct phylogenetic species. All 12 lineages were concordant with host specialization, but only three belonged to strictly defined morphological species. The estimation of the species tree obtained with Bayesian concordance analysis highlighted a potential co-evolutionary history between Melampsora species and their reciprocal aecial host plants. Within the Melampsora speciation process, aecial host may have had a strong effect on ancestral evolution, whereas telial host specificity seems to have evolved more recently. The morphological characters initially used to define species boundaries in the Melampsora genus are not reflective of the evolutionary and genetic relationships among poplar rusts. In order to construct a more meaningful taxonomy, host specificity must be considered an important criterion for delineating and describing species within the genus Melampsora as previously suggested by ecological species recognition.
Pascal Frey - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous monitoring and quantification of Melampsora allii-populina and Melampsora larici-populina on infected poplar leaves using a duplex real-time PCR assay
Plant Pathology, 2016Co-Authors: Cécile Guinet, Anne-laure Boutigny, Agathe Vialle, Pascal Frey, R. C. Hamelin, Renaud IoosAbstract:The rust fungi Melampsora larici-populina (Mlp) and Melampsora allii-populina (Map) are the main phytosanitary constraints for commercial poplar cultivation in Europe. Although Mlp is more aggressive and prevalent than Map, the two species may co-infect the same poplar tree or even the same poplar leaf, making the epidemiological surveys of each species difficult to achieve. In this study, a new duplex real-time PCR assay targeting each species was developed, based on single-copy genes. This test proved to be specific, inclusive and was successfully used to detect and quantify each species, starting from urediniospore samples or directly from infected poplar leaves, with or without visible symptoms. This new molecular tool was also assessed for comparative studies of time-course infection experiments on artificially inoculated poplar leaf discs. These studies showed that the growth dynamics of Map were significantly slower when the two species were co-inoculated on the same leaf disc, confirming that Map is less aggressive than Mlp.
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Simultaneous monitoring and quantification of Melampsora allii‐populina and Melampsora larici‐populina on infected poplar leaves using a duplex real‐time PCR assay
Plant Pathology, 2015Co-Authors: Cécile Guinet, Anne-laure Boutigny, Agathe Vialle, Pascal Frey, Richard C. Hamelin, Renaud IoosAbstract:The rust fungi Melampsora larici-populina (Mlp) and Melampsora allii-populina (Map) are the main phytosanitary constraints for commercial poplar cultivation in Europe. Although Mlp is more aggressive and prevalent than Map, the two species may co-infect the same poplar tree or even the same poplar leaf, making the epidemiological surveys of each species difficult to achieve. In this study, a new duplex real-time PCR assay targeting each species was developed, based on single-copy genes. This test proved to be specific, inclusive and was successfully used to detect and quantify each species, starting from urediniospore samples or directly from infected poplar leaves, with or without visible symptoms. This new molecular tool was also assessed for comparative studies of time-course infection experiments on artificially inoculated poplar leaf discs. These studies showed that the growth dynamics of Map were significantly slower when the two species were co-inoculated on the same leaf disc, confirming that Map is less aggressive than Mlp.
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A sensitive real-time PCR assay for the detection of the two Melampsora medusae formae speciales on infected poplar leaves
European Journal of Plant Pathology, 2013Co-Authors: Anne-laure Boutigny, Cécile Guinet, Agathe Vialle, Richard Hamelin, Pascal Frey, Renaud IoosAbstract:Melampsora medusae is a quarantine fungus in the European Union (EU) that causes a damaging leaf rust disease on poplars. Two formae speciales of the pathogen can be distinguished, M . medusae f. sp. deltoidae and M . medusae f. sp. tremuloidae , but the EU plant health directive 2000/29/EC currently in force does not make the distinction between them. EU countries must have the ability to detect and identify rapidly the introduction of these quarantine fungi and to conduct extensive surveys in case of outbreaks. Efficient detection tools are thus needed. In this study, a sensitive real-time PCR assay was developed to detect the presence of M . medusae in poplar leaf samples. A unique primer/hydrolysis probe combination targeting both formae speciales was designed using species-specific polymorphisms observed within the internal transcribed spacer region. An additional primer/hydrolysis probe combination was designed from a region of the 28S rDNA that is highly conserved in the genus Melampsora and used in a separate real-time PCR assay in order to check the quality of the DNA extracted from Melampsora urediniospores. The test developed demonstrated a high sensitivity since it enables the reproducible detection of two M . medusae urediniospore in a mixture of 2 mg of urediniospores (ca 800 000 urediniospores) of other Melampsora species. This new real-time PCR tool should be useful for laboratories in charge of official analyses since it has many advantages over the techniques currently used to monitor this quarantine pathogen in Europe.
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Optimization of a real-time PCR assay for the detection of the quarantine pathogen Melampsora medusae f. sp deltoidae
Fungal Biology, 2013Co-Authors: Anne-laure Boutigny, Claude Husson, Cécile Guinet, Agathe Vialle, Pascal Frey, Richard C. Hamelin, Axelle Andrieux, Renaud IoosAbstract:Melampsora medusae (Mm), one of the causal agents of poplar rust, is classified as an A2 quarantine pest for European Plant Protection Organization (EPPO) and its presence in Europe is strictly controlled. Two formae speciales have been described within Mm, Melampsora medusae f. sp. deltoidae (Mmd), and Melampsora medusae f. sp. tremuloidae (Mmt) on the basis of their pathogenicity on Populus species from the section Aigeiros (e.g. Populus deltoides) or Populus (e.g. Populus tremuloides), respectively. In this study, a real-time polymerase chain reaction (PCR) assay was developed allowing the detection of Mmd, the forma specialis that is economically harmful. A set of primers and hydrolysis probe were designed based on sequence polymorphisms in the large ribosomal RNA subunit (28S). The real-time PCR assay was optimized and performance criteria of the detection method, i.e. sensitivity, specificity, repeatability, reproducibility, and robustness, were assessed. The real-time PCR method was highly specific and sensitive and allowed the detection of one single urediniospore of Mmd in a mixture of 2 mg of urediniospores of other Melampsora species. This test offers improved specificity over currently existing conventional PCR tests and can be used for specific surveys in European nurseries and phytosanitary controls, in order to avoid introduction and spread of this pathogen in Europe.
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phylogenetic species recognition reveals host specific lineages among poplar rust fungi
Molecular Phylogenetics and Evolution, 2013Co-Authors: Agathe Vialle, Pascal Frey, Nicolas Feau, Louis Bernier, Richard C. HamelinAbstract:Fungal species belonging to the genus Melampsora (Basidiomycota, Pucciniales) comprise rust pathogens that alternate between Salicaceae and other plant hosts. Species delineation and identification are difficult within this group due to the paucity of observable morphological features. Several Melampsora rusts are highly host-specific and this feature has been used for identification at the species level. However, this criterion is not always reliable since different Melampsora rust species can overlap on one host but specialize on a different one. To date, two different species recognition methods are used to recognize and define species within the Melampsora genus: (i) morphological species recognition, which is based solely on morphological criteria; and (ii) ecological species recognition, which combines morphological criteria with host range to recognize and define species. In order to clarify species recognition within the Melampsora genus, we applied phylogenetic species recognition to Melampsora poplar rusts by conducting molecular phylogenetic analyses on 15 Melampsora taxa using six nuclear and mitochondrial loci. By assessing the genealogical concordance between phylogenies, we identified 12 lineages that evolved independently, corresponding to distinct phylogenetic species. All 12 lineages were concordant with host specialization, but only three belonged to strictly defined morphological species. The estimation of the species tree obtained with Bayesian concordance analysis highlighted a potential co-evolutionary history between Melampsora species and their reciprocal aecial host plants. Within the Melampsora speciation process, aecial host may have had a strong effect on ancestral evolution, whereas telial host specificity seems to have evolved more recently. The morphological characters initially used to define species boundaries in the Melampsora genus are not reflective of the evolutionary and genetic relationships among poplar rusts. In order to construct a more meaningful taxonomy, host specificity must be considered an important criterion for delineating and describing species within the genus Melampsora as previously suggested by ecological species recognition.
Agathe Vialle - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous monitoring and quantification of Melampsora allii-populina and Melampsora larici-populina on infected poplar leaves using a duplex real-time PCR assay
Plant Pathology, 2016Co-Authors: Cécile Guinet, Anne-laure Boutigny, Agathe Vialle, Pascal Frey, R. C. Hamelin, Renaud IoosAbstract:The rust fungi Melampsora larici-populina (Mlp) and Melampsora allii-populina (Map) are the main phytosanitary constraints for commercial poplar cultivation in Europe. Although Mlp is more aggressive and prevalent than Map, the two species may co-infect the same poplar tree or even the same poplar leaf, making the epidemiological surveys of each species difficult to achieve. In this study, a new duplex real-time PCR assay targeting each species was developed, based on single-copy genes. This test proved to be specific, inclusive and was successfully used to detect and quantify each species, starting from urediniospore samples or directly from infected poplar leaves, with or without visible symptoms. This new molecular tool was also assessed for comparative studies of time-course infection experiments on artificially inoculated poplar leaf discs. These studies showed that the growth dynamics of Map were significantly slower when the two species were co-inoculated on the same leaf disc, confirming that Map is less aggressive than Mlp.
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Simultaneous monitoring and quantification of Melampsora allii‐populina and Melampsora larici‐populina on infected poplar leaves using a duplex real‐time PCR assay
Plant Pathology, 2015Co-Authors: Cécile Guinet, Anne-laure Boutigny, Agathe Vialle, Pascal Frey, Richard C. Hamelin, Renaud IoosAbstract:The rust fungi Melampsora larici-populina (Mlp) and Melampsora allii-populina (Map) are the main phytosanitary constraints for commercial poplar cultivation in Europe. Although Mlp is more aggressive and prevalent than Map, the two species may co-infect the same poplar tree or even the same poplar leaf, making the epidemiological surveys of each species difficult to achieve. In this study, a new duplex real-time PCR assay targeting each species was developed, based on single-copy genes. This test proved to be specific, inclusive and was successfully used to detect and quantify each species, starting from urediniospore samples or directly from infected poplar leaves, with or without visible symptoms. This new molecular tool was also assessed for comparative studies of time-course infection experiments on artificially inoculated poplar leaf discs. These studies showed that the growth dynamics of Map were significantly slower when the two species were co-inoculated on the same leaf disc, confirming that Map is less aggressive than Mlp.
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A sensitive real-time PCR assay for the detection of the two Melampsora medusae formae speciales on infected poplar leaves
European Journal of Plant Pathology, 2013Co-Authors: Anne-laure Boutigny, Cécile Guinet, Agathe Vialle, Richard Hamelin, Pascal Frey, Renaud IoosAbstract:Melampsora medusae is a quarantine fungus in the European Union (EU) that causes a damaging leaf rust disease on poplars. Two formae speciales of the pathogen can be distinguished, M . medusae f. sp. deltoidae and M . medusae f. sp. tremuloidae , but the EU plant health directive 2000/29/EC currently in force does not make the distinction between them. EU countries must have the ability to detect and identify rapidly the introduction of these quarantine fungi and to conduct extensive surveys in case of outbreaks. Efficient detection tools are thus needed. In this study, a sensitive real-time PCR assay was developed to detect the presence of M . medusae in poplar leaf samples. A unique primer/hydrolysis probe combination targeting both formae speciales was designed using species-specific polymorphisms observed within the internal transcribed spacer region. An additional primer/hydrolysis probe combination was designed from a region of the 28S rDNA that is highly conserved in the genus Melampsora and used in a separate real-time PCR assay in order to check the quality of the DNA extracted from Melampsora urediniospores. The test developed demonstrated a high sensitivity since it enables the reproducible detection of two M . medusae urediniospore in a mixture of 2 mg of urediniospores (ca 800 000 urediniospores) of other Melampsora species. This new real-time PCR tool should be useful for laboratories in charge of official analyses since it has many advantages over the techniques currently used to monitor this quarantine pathogen in Europe.
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Optimization of a real-time PCR assay for the detection of the quarantine pathogen Melampsora medusae f. sp deltoidae
Fungal Biology, 2013Co-Authors: Anne-laure Boutigny, Claude Husson, Cécile Guinet, Agathe Vialle, Pascal Frey, Richard C. Hamelin, Axelle Andrieux, Renaud IoosAbstract:Melampsora medusae (Mm), one of the causal agents of poplar rust, is classified as an A2 quarantine pest for European Plant Protection Organization (EPPO) and its presence in Europe is strictly controlled. Two formae speciales have been described within Mm, Melampsora medusae f. sp. deltoidae (Mmd), and Melampsora medusae f. sp. tremuloidae (Mmt) on the basis of their pathogenicity on Populus species from the section Aigeiros (e.g. Populus deltoides) or Populus (e.g. Populus tremuloides), respectively. In this study, a real-time polymerase chain reaction (PCR) assay was developed allowing the detection of Mmd, the forma specialis that is economically harmful. A set of primers and hydrolysis probe were designed based on sequence polymorphisms in the large ribosomal RNA subunit (28S). The real-time PCR assay was optimized and performance criteria of the detection method, i.e. sensitivity, specificity, repeatability, reproducibility, and robustness, were assessed. The real-time PCR method was highly specific and sensitive and allowed the detection of one single urediniospore of Mmd in a mixture of 2 mg of urediniospores of other Melampsora species. This test offers improved specificity over currently existing conventional PCR tests and can be used for specific surveys in European nurseries and phytosanitary controls, in order to avoid introduction and spread of this pathogen in Europe.
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phylogenetic species recognition reveals host specific lineages among poplar rust fungi
Molecular Phylogenetics and Evolution, 2013Co-Authors: Agathe Vialle, Pascal Frey, Nicolas Feau, Louis Bernier, Richard C. HamelinAbstract:Fungal species belonging to the genus Melampsora (Basidiomycota, Pucciniales) comprise rust pathogens that alternate between Salicaceae and other plant hosts. Species delineation and identification are difficult within this group due to the paucity of observable morphological features. Several Melampsora rusts are highly host-specific and this feature has been used for identification at the species level. However, this criterion is not always reliable since different Melampsora rust species can overlap on one host but specialize on a different one. To date, two different species recognition methods are used to recognize and define species within the Melampsora genus: (i) morphological species recognition, which is based solely on morphological criteria; and (ii) ecological species recognition, which combines morphological criteria with host range to recognize and define species. In order to clarify species recognition within the Melampsora genus, we applied phylogenetic species recognition to Melampsora poplar rusts by conducting molecular phylogenetic analyses on 15 Melampsora taxa using six nuclear and mitochondrial loci. By assessing the genealogical concordance between phylogenies, we identified 12 lineages that evolved independently, corresponding to distinct phylogenetic species. All 12 lineages were concordant with host specialization, but only three belonged to strictly defined morphological species. The estimation of the species tree obtained with Bayesian concordance analysis highlighted a potential co-evolutionary history between Melampsora species and their reciprocal aecial host plants. Within the Melampsora speciation process, aecial host may have had a strong effect on ancestral evolution, whereas telial host specificity seems to have evolved more recently. The morphological characters initially used to define species boundaries in the Melampsora genus are not reflective of the evolutionary and genetic relationships among poplar rusts. In order to construct a more meaningful taxonomy, host specificity must be considered an important criterion for delineating and describing species within the genus Melampsora as previously suggested by ecological species recognition.
Makoto Kakishima - One of the best experts on this subject based on the ideXlab platform.
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Life cycle and taxonomy of Melampsora abietis-populi in China and its phylogenetic position in Melampsora on Populus
Mycological Progress, 2020Co-Authors: Makoto KakishimaAbstract:Rust diseases of Abies and Populus caused by Melampsora abietis-populi are observed in the forests of northeastern Asia. However, the life cycle and distribution of this species are not sufficiently clarified in China. The heteroecious life cycle alternating between spermogonial and aecial stages on A. holophylla and uredinial and telial stages on Populus pseudosimonii is confirmed for the first time in China by inoculations and phylogenetic analyses. The identity of these stages with M. abietis-populi is also confirmed by comparative morphology with specimens from Japan and phylogenetic analyses. These plants are new hosts for this species. Host plants and distribution of this species in China are described based on specimens obtained from the field and from inoculations. The phylogenetic position of this species within Melampsora is clarified for the first time. Based on morphological similarities and phylogenetic analyses, M. nujiangensis reported on Populus yunnanensis is treated as a synonym of M. abietis-populi.
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Melampsora salicis-bakko , a new species on willows in Japan evidenced by morphological and molecular phylogenetic analyses
Mycological Progress, 2016Co-Authors: Peng Zhao, Qing-hong Wang, Cheng-ming Tian, Qi Wang, Makoto KakishimaAbstract:Through the morphological and molecular examinations of Melampsora species on willows, we clarified the taxonomic identity of the rust specimens on Salix bakko, S. hultenii and S. leucopithecia from Japan and described the following rust fungus as a new species, Melampsora salicis-bakko. This rust fungus resembled M. caprearum in morphology of teliospores, but it differed from M. caprearum mainly in the density of spines on the urediniospores. Molecular phylogenetic analyses using the rDNA ITS region (complete ITS1, 5.8S rRNA gene and ITS2) revealed that M. salicis-bakko was monophyletic, and that this rust fungus was distinct from other Melampsora species, including M. caprearum.
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Integrating a Numerical Taxonomic Method and Molecular Phylogeny for Species Delimitation of Melampsora Species (Melampsoraceae, Pucciniales) on Willows in China
PloS one, 2015Co-Authors: Peng Zhao, Qing-hong Wang, Cheng-ming Tian, Makoto KakishimaAbstract:The species in genus Melampsora are the causal agents of leaf rust diseases on willows in natural habitats and plantations. However, the classification and recognition of species diversity are challenging because morphological characteristics are scant and morphological variation in Melampsora on willows has not been thoroughly evaluated. Thus, the taxonomy of Melampsora species on willows remains confused, especially in China where 31 species were reported based on either European or Japanese taxonomic systems. To clarify the species boundaries of Melampsora species on willows in China, we tested two approaches for species delimitation inferred from morphological and molecular variations. Morphological species boundaries were determined based on numerical taxonomic analyses of morphological characteristics in the uredinial and telial stages by cluster analysis and one-way analysis of variance. Phylogenetic species boundaries were delineated based on the generalized mixed Yule-coalescent (GMYC) model analysis of the sequences of the internal transcribed spacer (ITS1 and ITS2) regions including the 5.8S and D1/D2 regions of the large nuclear subunit of the ribosomal RNA gene. Numerical taxonomic analyses of 14 morphological characteristics recognized in the uredinial-telial stages revealed 22 morphological species, whereas the GMYC results recovered 29 phylogenetic species. In total, 17 morphological species were in concordance with the phylogenetic species and 5 morphological species were in concordance with 12 phylogenetic species. Both the morphological and molecular data supported 14 morphological characteristics, including 5 newly recognized characteristics and 9 traditionally emphasized characteristics, as effective for the differentiation of Melampsora species on willows in China. Based on the concordance and discordance of the two species delimitation approaches, we concluded that integrative taxonomy by using both morphological and molecular variations was an effective approach for delimitating Melampsora species on willows in China.
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A morphological and molecular survey of Japanese Melampsora species on willows reveals a new species and two new records
Mycological Progress, 2015Co-Authors: Peng Zhao, Qing-hong Wang, Cheng-ming Tian, Qi Wang, Yuichi Yamaoka, Makoto KakishimaAbstract:During morphological and molecular phylogenetic studies of Melampsora species on willows, one novel species, Melampsora salicis-reinii sp. nov., and two new records, M. ribesii-viminalis and M. ribesii-purpureae, are described from Japan. Melampsora salicis-reinii on Salix reinii was characterized by its amphigenous telia and ellipsoid urediniospores with echinulate spines on a rugose surface. Phylogenetic results using the rDNA ITS region (complete ITS1, 5.8S rRNA gene and ITS2) supported morphological elucidation, and both morphological and molecular evidences supported M. salicis-reinii as a new species. In addition, M. ribesii-viminalis was identified on S. pet-susu and S. sachalinensis, and M. ribesii-purpureae was recognized on S. integra. These two Melampsora species were reported as new records in Japan for the first time.
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Two new species and one new record of Melampsora on willows from China
Mycological Progress, 2015Co-Authors: Peng Zhao, Cheng-ming Tian, Qi Wang, Yuichi Yamaoka, Yi-jian Yao, Makoto KakishimaAbstract:Two new species of rust fungi, Melampsora salicis-purpureae sp. nov. and M. salicis-argyraceae sp. nov., and a new record, M. iranica, are described from China based on morphological characteristics and molecular phylogeny of the internal transcribed spacer (ITS1 and ITS2) regions including 5.8S of the ribosomal RNA gene. Melampsora salicis-purpureae on Salix purpurea was characterized by amphigenous uredinia, subepidermal teliospores, and relatively shorter teliospores and amphigenous telia. Melampsora salicis-argyraceae on S. argyaceae possessed sparse spines on urediniospores, epiphyllous telia and subepidermal teliospores with slightly thickened apex. In addition, specimens on S. alba and S. psammophyla were identified as M. iranica.
Lars Ericson - One of the best experts on this subject based on the ideXlab platform.
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spatial variation in resistance and virulence in the host pathogen system salix triandra Melampsora amygdalinae
Journal of Ecology, 2006Co-Authors: Lena Niemi, Anders Wennström, Joakim Hjältén, Patrik Waldmann, Lars EricsonAbstract:Spatial variation in resistance and virulence in the host-pathogen system Salix triandra-Melampsora amygdalinae
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Spatial variation in resistance and virulence in the host–pathogen system Salix triandra–Melampsora amygdalinae
Journal of Ecology, 2006Co-Authors: Lena Niemi, Anders Wennström, Joakim Hjältén, Patrik Waldmann, Lars EricsonAbstract:Spatial variation in resistance and virulence in the host-pathogen system Salix triandra-Melampsora amygdalinae