The Experts below are selected from a list of 162 Experts worldwide ranked by ideXlab platform
Walter L Murfee - One of the best experts on this subject based on the ideXlab platform.
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a novel ex vivo mouse Mesometrium culture model for investigating angiogenesis in microvascular networks
Journal of Vascular Research, 2018Co-Authors: Ariana D Suarezmartinez, Susanne Bierschenk, Katie Huang, Dana Kaplan, Carolyn L Bayer, Stryder M Meadows, Markus Sperandio, Walter L MurfeeAbstract:Background: The development of models that incorporate intact microvascular networks enables the investigation of multicellular dynamics during angiogenesis. Our laboratory introduced the rat mesentery culture model as such a tool, which would be enhanced with mouse tissue. Since mouse mesentery is avascular, an alternative is mouse Mesometrium, the connective tissue of uterine horns. The study's objective was to demonstrate that mouse Mesometrium contains microvascular networks that can be cultured to investigate multicellular dynamics during angiogenesis. Methods: Harvested Mesometrium tissues from C57131/6 female mice were cultured in media with serum for up to 7 days. PECAM, NG2, aSMA, and LYVE-1 labeling identified endothelial cells, pericytes, smooth muscle cells, and lymphatic endothelial cells, respectively. Results: These cells comprised microvascular networks with arterioles, venules, and capillaries. Compared to day 0, capillary sprouts per vascular length were increased by 3 and 5 days in culture (day 0, 0.08 +/- 0.01;day 3, 3.19 +/- 0.78;day 5, 2.49 +/- 0.05 sprouts/mm;p < 0.05). Time-lapse imaging of cultured tissues from FIkEGFP mice showcases the use of the model for lineage studies. The impact is supported by the identification of endothelial cell jumping from one sprout to another. Conclusion: These results introduce a novel culture model for investigating multicellular dynamics during angiogenesis in real-time ex vivo microvascular networks.
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development of the ex vivo mouse Mesometrium model to investigate multicellular dynamics during angiogenesis
The FASEB Journal, 2017Co-Authors: Ariana D Suarezmartinez, Susanne Bierschenk, Katie Huang, Dana Kaplan, Stryder M Meadows, Markus Sperandio, Walter L MurfeeAbstract:The development of biomimetic in vitro or ex vivo models that can incorporate the complexity of intact microvascular networks would enable the investigation of multicellular dynamics associated wit...
Ariana D Suarezmartinez - One of the best experts on this subject based on the ideXlab platform.
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a novel ex vivo mouse Mesometrium culture model for investigating angiogenesis in microvascular networks
Journal of Vascular Research, 2018Co-Authors: Ariana D Suarezmartinez, Susanne Bierschenk, Katie Huang, Dana Kaplan, Carolyn L Bayer, Stryder M Meadows, Markus Sperandio, Walter L MurfeeAbstract:Background: The development of models that incorporate intact microvascular networks enables the investigation of multicellular dynamics during angiogenesis. Our laboratory introduced the rat mesentery culture model as such a tool, which would be enhanced with mouse tissue. Since mouse mesentery is avascular, an alternative is mouse Mesometrium, the connective tissue of uterine horns. The study's objective was to demonstrate that mouse Mesometrium contains microvascular networks that can be cultured to investigate multicellular dynamics during angiogenesis. Methods: Harvested Mesometrium tissues from C57131/6 female mice were cultured in media with serum for up to 7 days. PECAM, NG2, aSMA, and LYVE-1 labeling identified endothelial cells, pericytes, smooth muscle cells, and lymphatic endothelial cells, respectively. Results: These cells comprised microvascular networks with arterioles, venules, and capillaries. Compared to day 0, capillary sprouts per vascular length were increased by 3 and 5 days in culture (day 0, 0.08 +/- 0.01;day 3, 3.19 +/- 0.78;day 5, 2.49 +/- 0.05 sprouts/mm;p < 0.05). Time-lapse imaging of cultured tissues from FIkEGFP mice showcases the use of the model for lineage studies. The impact is supported by the identification of endothelial cell jumping from one sprout to another. Conclusion: These results introduce a novel culture model for investigating multicellular dynamics during angiogenesis in real-time ex vivo microvascular networks.
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development of the ex vivo mouse Mesometrium model to investigate multicellular dynamics during angiogenesis
The FASEB Journal, 2017Co-Authors: Ariana D Suarezmartinez, Susanne Bierschenk, Katie Huang, Dana Kaplan, Stryder M Meadows, Markus Sperandio, Walter L MurfeeAbstract:The development of biomimetic in vitro or ex vivo models that can incorporate the complexity of intact microvascular networks would enable the investigation of multicellular dynamics associated wit...
Cláudio Alvarenga De Oliveira - One of the best experts on this subject based on the ideXlab platform.
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morfologia do sistema genital feminino da paca cuniculus paca linnaeus 1766
Brazilian Journal of Veterinary Research and Animal Science, 2011Co-Authors: Ana Carolina Goncalves Dos Reis, Carolina Scanavez Martins, Silvia Helena Brendolan Gerbasi, Marcia Rita Fernandes Machado, Cláudio Alvarenga De OliveiraAbstract:The macroscopic morphology of genital tract of six adult female paca (Cuniculus paca) was studied through dissection of abdominal and pelvic cavities immediately after death. The ovaries have ovoid shape, dorsal-ventrally flattened, whitish yellow color with small red spots on its surface, located in sublombar situation, caudally to the kidneys, surrounded by a shallow bursa and fixed by the mesovarium; the oviduct is a pair of sinuous tubes, continuous with the ovaries, inserted in the mesosalpinx and extending up to the beginning of each correspondent uterine horn. The uterus horns are rectilinear and attached in the abdominal walls by the Mesometrium, each horns extends caudally into the pelvic cavity and are linked by intercornual ligament, the ventral surface chiefly contacts the bladder; two cervices are present, although the uterine septum is incomplete, characterizing the presence of two internal uterine ostia and one external uterine ostium, considering this uterus as an incomplete duplex. The vagina is a tubular organ that is positioned ventrally to the rectum and dorsally to the urinary bladder and urethra; urethral duct is placed apart of the vaginal canal, characterizing the absence of the vestibulum; the vulva presents itself planed, with reduced vulvae lips, the clitoris is a conical structure with two prominent pointed structures at its distal region. There were no differences statistically significant in measurements made in the ovaries, oviducts and uterine horns, when comparing the right and left sides.
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Morfologia do sistema genital feminino da paca (Cuniculus paca, Linnaeus, 1766) Morphology of the female genital system of the paca (Cuniculus paca, Linnaeus, 1766)
2011Co-Authors: Silvia Helena, Brendolan Gerbasi, Carolina Scanavez Martins, Cláudio Alvarenga De OliveiraAbstract:The macroscopic morphology of genital tract of six adult female paca ( Cuniculus paca) was studied through dissection of abdominal and pelvic cavities immediately after death. The ovaries have ovoid shape, dorsal-ventrally flattened, whitish yellow color with small red spots on its surface, located in sublombar situation, caudally to the kidneys, surrounded by a shallow bursa and fixed by the mesovarium; the oviduct is a pair of sinuous tubes, continuous with the ovaries, inserted in the mesosalpinx and extending up to the beginning of each correspondent uterine horn. The uterus horns are rectilinear and attached in the abdominal walls by the Mesometrium, each horns extends caudally into the pelvic cavity and are linked by intercornual ligament, the ventral surface chiefly contacts the bladder; two cervices are present, although the uterine septum is incomplete, characterizing the presence of two internal uterine ostia and one external uterine ostium, considering this uterus as an incomplete duplex. The vagina is a tubular organ that is positioned ventrally to the rectum and dorsally to the urinary bladder and urethra; urethral duct is placed apart of the vaginal canal, characterizing the absence of the vestibulum; the vulva presents itself planed, with reduced vulvae lips, the clitoris is a conical structure with two prominent pointed structures at its distal region. There were no differences statistically significant in measurements made in the ovaries, oviducts and uterine horns, when comparing the right and left sides.
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Morfologia do sistema genital feminino da paca (Cuniculus paca, Linnaeus, 1766)
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia, 2011Co-Authors: Reis, Ana Carolina Gonçalves Dos, Gerbasi, Silvia Helena Brendolan, Martins Carolina, Machado, Márcia Rita Fernandes, Cláudio Alvarenga De OliveiraAbstract:The macroscopic morphology of genital tract of six adult female paca (Cuniculus paca) was studied through dissection of abdominal and pelvic cavities immediately after death. The ovaries have ovoid shape, dorsal-ventrally flattened, whitish yellow color with small red spots on its surface, located in sublombar situation, caudally to the kidneys, surrounded by a shallow bursa and fixed by the mesovarium; the oviduct is a pair of sinuous tubes, continuous with the ovaries, inserted in the mesosalpinx and extending up to the beginning of each correspondent uterine horn. The uterus horns are rectilinear and attached in the abdominal walls by the Mesometrium, each horns extends caudally into the pelvic cavity and are linked by intercornual ligament, the ventral surface chiefly contacts the bladder; two cervices are present, although the uterine septum is incomplete, characterizing the presence of two internal uterine ostia and one external uterine ostium, considering this uterus as an incomplete duplex. The vagina is a tubular organ that is positioned ventrally to the rectum and dorsally to the urinary bladder and urethra; urethral duct is placed apart of the vaginal canal, characterizing the absence of the vestibulum; the vulva presents itself planed, with reduced vulvae lips, the clitoris is a conical structure with two prominent pointed structures at its distal region. There were no differences statistically significant in measurements made in the ovaries, oviducts and uterine horns, when comparing the right and left sides.Foi caracterizada a morfologia macroscópica do genital feminino, de seis exemplares adultos de paca (Cuniculus paca), mediante dissecação das cavidades abdominal e pélvica imediatamente após o óbito. Os ovários apresentam forma ovoide, achatados dorso-ventralmente, de coloração amarela esbranquiçada com pequenos pontos avermelhados em sua superfície; têm localização sublombar, caudal aos rins; estão envoltos por uma rasa bolsa ovárica e fixados pelo mesovário; a tuba uterina é um órgão par, de aspecto sinuoso, contínua aos ovários, estando inserida na mesossalpinge e se estendendo até o início de cada corno uterino correspondente. Os cornos uterinos retilíneos fixam-se à parede abdominal pelo mesométrio e se unem pelo ligamento intercornual na altura da entrada da pelve, onde se posicionam dorsalmente à vesícula urinária; duas cevices estão presentes, embora o septo uterino que as separa seja incompleto, caracterizando presença de dois óstios uterinos internos e um único óstio uterino externo, considerando-se este útero como duplo incompleto. A vagina é um órgão tubular que se posiciona ventral ao reto e dorsal à vesícula urinária e à uretra, não se verificou a presença de vestíbulo e a vagina e a uretra não possuem ponto comum de convergência, abrindo-se, cada uma delas, diretamente na região vulvar, que se apresenta plana, com reduzidos lábios vulvares, apenas o clitóris de forma cônica é pouco proeminente e apresenta duas estruturas pontiagudas em sua região distal. Não se verificaram diferenças estatisticamente significativas nas mensurações realizadas nos ovários, tubas uterinas e cornos uterinos, ao se comparar os antímeros direito e esquerdo
F.a. Ahmed - One of the best experts on this subject based on the ideXlab platform.
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Morphology and Morphometry of Female Genital System of Mizo Local Pig (Zovawk)
2015Co-Authors: A. Kalita, Pranab Chandra Kalita, P.j. Doley, F.a. AhmedAbstract:The present investigation provides a baseline data on gross anatomy of female reproductive system of Mizo local pig (Zovawk). The organs can be divided functionally and morphologically into the ovaries, and the tubular organs, which lead from the ovaries through the pelvis to the outside. The tubular organs consist of the uterine tubes, the uterus, and the copulatory organ. The copulatory organ is subdivided into vagina, vestibule and the vulva. The uterus is apparently U-shaped and the diverging horns were long, flexuous and almost reach the caudal extremities of the ovaries. The mesovarium was long. The mesosalpinx was highly vascular, arises from the lateral surface of the mesovarium and the adjacent part of the Mesometrium. It contained the uterine tube and formed a large cone-shaped ovarian bursa which concealed the ovary. The Mesometrium was attached dorso-laterally to the uterine horns and corpus uteri, originating from the dorso-lateral walls of the abdominal and pelvic peritoneal cavity. The cervix uteri were not covered by peritoneum. The overall biometrical value in respect of organs of female genital system in the present study was lower than the common large breed of pigs. Key words: Morphology, Morphometry, Female genital system, Mizo local pi
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Morphology and Morphometry of Female Genital System of Mizo Local Pig (Zovawk)
International Journal of Research, 2014Co-Authors: Kalita, Pranab Chandra Kalita, P.j. Doley, F.a. AhmedAbstract:The present investigation provides a baseline data on gross anatomy of female reproductive system of Mizo local pig (Zo vawk). The organs can be divided functionally and morphologically into the ovaries, and the tubular organs, which lead from the ovaries through the pelvis to the outside. The tubular organs consist of the uterine tubes, the uterus, and the copulatory organ. The copulatory organ is subdivided into vagina, vestibule and the vulva. The uterus is apparently U-shaped and the diverging horns were long, flexuous and almost reach the caudal extremities of the ovaries. The mesovarium was long. The mesosalpinx was highly vascular, arises from the lateral surface of the mesovarium and the adjacent part of the Mesometrium. It contained the uterine tube and formed a large cone-shaped ovarian bursa which concealed the ovary. The Mesometrium was attached dorso-laterally to the uterine horns and corpus uteri, originating from the dorso-lateral walls of the abdominal and pelvic peritoneal cavity. The cervix uteri were not covered by peritoneum. The overall biometrical value in respect of organs of female genital system in the present study was lower than the common large breed of pigs.
Sushila Maan - One of the best experts on this subject based on the ideXlab platform.
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virological immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in ifnar1 blocked mice during early and mid gestation
Scientific Reports, 2020Co-Authors: Mani Saminathan, Karanveer Singh, S Vineetha, Madhulina Maity, S K Biswas, G B Manjunathareddy, H C Chauhan, A A P Milton, M A Ramakrishnan, Sushila MaanAbstract:Transplacental transmission (TPT) of wild-type Indian BTV-1 had never been experimentally proved. This study was first time investigated TPT of Indian BTV-1 (isolated from aborted and stillborn goat fetal spleens). The sequential pathology, virological and immune cell kinetics (CD4+, CD8+ T-lymphocytes and NK cells in spleen and PBMCs), and apoptosis in IFNAR1-blocked pregnant mice during early (infected on 1 GD) and mid (infected on 8 GD) gestation have been studied. There was higher rate of TPT during mid stage (71.43%) than early (57.14%) stage. In early stage reduced implantation sites, early embryonic deaths, abortions, and necro-haemorrhagic lesions had observed. Mid stage, congenital defects and neurological lesions in foetuses like haemorrhages, diffuse cerebral edema, necrotizing encephalitis and decreased bone size (Alizarin red staining) were noticed. BTV-1 antigen was first time demonstrable in cells of Mesometrium, decidua of embryos, placenta, uterus, ovary, and brain of foetuses by immunohistochemistry and quantified by real-time qRT-PCR. BTV-inoculated mice were seroconverted by 7 and 5 dpi, and reached peak levels by 15 and 9 dpi in early and mid gestation, respectively. CD4+ and CD8+ cells were significantly decreased (increased ratio) on 7 dpi but subsequently increased on 15 dpi in early gestation. In mid gestation, increased CD8+ cells (decreased ratio) were observed. Apoptotic cells in PBMCs and tissues increased during peak viral load. This first time TPT of wild-type Indian BTV-1 deserves to be reported for implementation of control strategies. This model will be very suitable for further research into mechanisms of TPT, overwintering, and vaccination strategies.