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Marek Lukasz Roszko - One of the best experts on this subject based on the ideXlab platform.
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DOI 10.1095/biolreprod.113.112813 Intersex Gonad Differentiation in Cultured Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) Sturgeon1
2016Co-Authors: Małgorzata Rzepkowska, Teresa Ostaszewska, Monika Gibala, Marek Lukasz RoszkoAbstract:Among sturgeons, the occurrence of individuals with gonads containing both testis and ovary components is considered pathological, and such fish are described as intersex individuals or intersexes. Intersexes are observed in both wild and cultured populations of sturgeon, usually at low frequencies. In the present study, intersex Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeons constituted 30 % of the studied populations. Macroscopically, intersex gonads were recognizable from 500 days posthatching (dph). Initially, gonads with predominantly male characteristics (testis-ova) were observed, but in older fish gonads with predominantly female traits (ova-testis) were more frequent. Using microscopic analysis, intersex gonads were discernible by 130–200 dph. Observations of intersex germinal epithelium development and analysis of sex distribution in the study populations indicated that feminization was occurring. Histological analysis revealed that differentiation of the germinal epithelium in such gonads was accompanied by various morphological alterations (trans-formations) that were described using quantitative and locali-zation criteria. The most common type of transformations, massive subepithelial transformations, was manifested by the presence of abundant female germinal tissue located under the gonad surface epithelium in the developing testis. These transformations were identified in the early development stage (100–200 dph). In this type of transformation, differentiation of female germinal tissue at the gonad surface and male tissue at the Mesorchium/mesovarium resulted in complete formation of both male and female germinal epithelia within the same gonad. feminization, germinal epithelium, gonad, intersex, sturgeo
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Intersex gonad differentiation in cultured Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon 1 Running title: Intersex gonad differentiation in sturgeons
2014Co-Authors: Małgorzata Rzepkowska, Teresa Ostaszewska, Monika Gibala, Marek Lukasz RoszkoAbstract:Among sturgeons, the occurrence of individuals with gonads containing both testis and ovary component is considered a pathological, and such fish are described as intersex individuals or intersexes. Intersexes are observed in both wild and cultured populations of sturgeon, usually at low frequencies. In the present study, intersex Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeons constituted 30% of the studied populations. Macroscopically, intersex gonads were recognizable from the 500 days post hatching (dph). Initially, gonads of predominantly male characteristics (testis-ova) were observed but, in older fish, gonads with predominantly female traits (ova-testis) were more frequent. Using microscopic analysis, intersex gonads were discernible by 130-200 dph. Observations of intersex germinal epithelium development and analysis of sex the distribution in the study populations indicated that feminization was occurring. Histological analysis revealed that differentiation of the germinal epithelium in such gonads was accompanied by various morphological alterations (transformations) that were described using quantitative and localization criteria. The most common type of transformations, massive sub-epithelial transformations were manifested by the presence of abundant female germinal tissue located under the gonad surface epithelium in the developing testis. These transformations were identified in the early development stage (100–200 dph). In this type of transformation, differentiation of female germinal tissue at the gonad surface, and male tissue at the Mesorchium/mesovarium resulted in complete formation of both, male and female germinal epithelia within the same gonad.
Winarto Adi - One of the best experts on this subject based on the ideXlab platform.
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Anatomi Organ Reproduksi Jantan Biawak Air Asia, Varanus salvator (Reptil: Varanidae)
'Institut Pertanian Bogor', 2016Co-Authors: . Mahfud, Nisa’ Chairun, Winarto AdiAbstract:Indonesia merupakan negara dengan tingkat eksploitasi biawak V. salvator terbesar di dunia yang sebagian besar untuk melayani permintaan perdagangan kulit. Tingginya permintaan kulit biawak di Indonesia mengkhawatirkan menyebabkan turunnya populasi satwa tersebut. Penelitian ini bertujuan untuk mempelajari anatomi organ reproduksi jantan biawak air asia (Varanus salvator) (Reptil: Varanidae). Dua ekor biawak jantan dewasa digunakan dalam penelitian ini. Hewan dianestesi, dilakukan exanguinasi, dan difiksasi dengan larutan paraformaldehida 4% secara perfusi. Pengamatan dilakukan terhadap situs viscerum, morfologi, dan morfometri organ reproduksi mulai testis sampai hemipenis. Secara makroskopis, organ reproduksi jantan V. salvator terdiri atas testis, ductus epididymidis, ductus deferens dan hemipenis yang masing-masing berjumlah sepasang. Posisi testis menggantung di dinding dorsal coelom melalui Mesorchium. Dari bagian dorsal testis terdapat ductus epididymidis yang panjangsampai di ujung kaudal ginjal. Ductus deferens, berupa saluran kecil, lurus dan berakhir di ujung hemipenis yang terletak di dalam pangkal ekor. Pada bagian kranial hemipenis ditutupi oleh papillae berbentuk konikal. Di kaudal dari hemipenis ditemukan otot retraktor yang memanjang ke arah ekor, dan diduga berperan menarik hemipenis ke dalam setelah kopulasi. Organ reproduksi jantan biawak secara umum mirip dengan reptilia lain khususnya ular dan kadal, dengan karakteristik adanya sepasang hemipenis.Kata kunci: Varanidae, Varanus salvator, organ reproduksi jantan, hemipenis, otot retraktor. (Anatomy of The Male Reproductive Organ of Water Monitor, Varanus salvator (Reptil: Varanidae))Indonesia is a country with high levels of exploitation of Varanus salvator that mainly serve the demand of leather trade. The high demand of lizard leather in Indonesian was alarming, cause a decline population of these animals. To improve our understanding on reproduction organs of the animal, we conduct this anatomical study. The study was used two adult male lizards. The animals were anesthetized, exanguinated and fixated in 4% paraformaldehyde by tissue perfusion method. Observations were performed to the visceral site, morphological and morphometrical of the male reproductive organs, from testes to hemipenes. Macroscopically, male reproductive organs of V. salvator were a pair of testes, epididymidis ducts, deferens ducts and hemipenes. The testis attached to dorsal wall of the coelom and fixed by the Mesorchium. The epididymidis duct was long tubes that located in the dorsal of testes, winding up at the caudal end of the kidney. The deferens duct was a small duct, running straight and last at the end of each hemipenis, located at the base of the tail. The cranial part of each hemipenis was covered by conical shaped papillae. Furthermore, at the caudal of each hemipenis was found the retractor muscle that extends toward the tail, and is thought to contribute to the retracting hemipenis after copulation. The male reproductive organs of V. salvator are generally similar to the other reptiles, especially snakes and lizards, with peculiar a pair of hemipenes.Keywords: Varanidae, Varanus salvator, male reproductive organs, hemipenes, retractor muscles
J. H. Tramezzani - One of the best experts on this subject based on the ideXlab platform.
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NPY- and CGRP-like immunoreactive nerve fibers in the testis and Mesorchium of the toad (Bufo arenarum)
Cell and Tissue Research, 1995Co-Authors: M. V. Achi, J. M. Figueroa, V. González Nicolini, M. J. Villar, J. H. TramezzaniAbstract:The presence and distribution of peptidergic nerve fibers were studied in the testis and Mesorchium of the toad by means of immunohistochemistry. Cryostat sections of the testis and whole-mount preparations of mesorchia were immunostained with antisera to calcitonin gene-related peptide (CGRP) and neuropeptide tyrosine (NPY). After leaving the Mesorchium CGRP-immunoreactive (IR) fibers were seen predominantly running in between the seminiferous tubules. In addition, a small population of CGRP-IR nerve fibers formed thin plexuses around blood vessels. Conversely, NPY-like immunoreactivity predominated in nerve fibers that formed dense plexuses around vessels both in the Mesorchium and testis. Additionally, some single NPY-IR nerve fibers could be seen in both structures studied. The functional significance of these peptidergic systems in the testis of the toad remains to be analyzed.
Małgorzata Rzepkowska - One of the best experts on this subject based on the ideXlab platform.
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DOI 10.1095/biolreprod.113.112813 Intersex Gonad Differentiation in Cultured Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) Sturgeon1
2016Co-Authors: Małgorzata Rzepkowska, Teresa Ostaszewska, Monika Gibala, Marek Lukasz RoszkoAbstract:Among sturgeons, the occurrence of individuals with gonads containing both testis and ovary components is considered pathological, and such fish are described as intersex individuals or intersexes. Intersexes are observed in both wild and cultured populations of sturgeon, usually at low frequencies. In the present study, intersex Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeons constituted 30 % of the studied populations. Macroscopically, intersex gonads were recognizable from 500 days posthatching (dph). Initially, gonads with predominantly male characteristics (testis-ova) were observed, but in older fish gonads with predominantly female traits (ova-testis) were more frequent. Using microscopic analysis, intersex gonads were discernible by 130–200 dph. Observations of intersex germinal epithelium development and analysis of sex distribution in the study populations indicated that feminization was occurring. Histological analysis revealed that differentiation of the germinal epithelium in such gonads was accompanied by various morphological alterations (trans-formations) that were described using quantitative and locali-zation criteria. The most common type of transformations, massive subepithelial transformations, was manifested by the presence of abundant female germinal tissue located under the gonad surface epithelium in the developing testis. These transformations were identified in the early development stage (100–200 dph). In this type of transformation, differentiation of female germinal tissue at the gonad surface and male tissue at the Mesorchium/mesovarium resulted in complete formation of both male and female germinal epithelia within the same gonad. feminization, germinal epithelium, gonad, intersex, sturgeo
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Intersex gonad differentiation in cultured Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon 1 Running title: Intersex gonad differentiation in sturgeons
2014Co-Authors: Małgorzata Rzepkowska, Teresa Ostaszewska, Monika Gibala, Marek Lukasz RoszkoAbstract:Among sturgeons, the occurrence of individuals with gonads containing both testis and ovary component is considered a pathological, and such fish are described as intersex individuals or intersexes. Intersexes are observed in both wild and cultured populations of sturgeon, usually at low frequencies. In the present study, intersex Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeons constituted 30% of the studied populations. Macroscopically, intersex gonads were recognizable from the 500 days post hatching (dph). Initially, gonads of predominantly male characteristics (testis-ova) were observed but, in older fish, gonads with predominantly female traits (ova-testis) were more frequent. Using microscopic analysis, intersex gonads were discernible by 130-200 dph. Observations of intersex germinal epithelium development and analysis of sex the distribution in the study populations indicated that feminization was occurring. Histological analysis revealed that differentiation of the germinal epithelium in such gonads was accompanied by various morphological alterations (transformations) that were described using quantitative and localization criteria. The most common type of transformations, massive sub-epithelial transformations were manifested by the presence of abundant female germinal tissue located under the gonad surface epithelium in the developing testis. These transformations were identified in the early development stage (100–200 dph). In this type of transformation, differentiation of female germinal tissue at the gonad surface, and male tissue at the Mesorchium/mesovarium resulted in complete formation of both, male and female germinal epithelia within the same gonad.
Waldemar S Costa - One of the best experts on this subject based on the ideXlab platform.
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ANATOMIC ASPECTS OF EPIDIDYMIS AND TUNICA VAGINALIS IN PATIENTS WITH TESTICULAR TORSION
2015Co-Authors: Tunica Vaginalis, Luciano A Favorito, Andre G Cavalcante, In Testicular, Torsionpediatric Urology, Waldemar S CostaAbstract:Objective: To analyze the morphology of epididymis and tunica vaginalis as well as their anatomical anomalies in patients with testicular torsion. Materials and Methods: We studied 25 patients (50 testes) aged between 12 and 23 years (mean 15.6). Torsion length ranged from 2 hours to 2 days (mean 8 hours). Epididymal anatomy was classified in 6 groups: Type I- epididymis united to the testis by its head and tail; Type II- epididymis totally united to the testis; Type III- disjunction of epididymal tail; Type IV- disjunction of epididy-mal head; Type V- total disjunction between testis and epididymis, and Type VI- epididymal atresia. The type of torsion was classified in 3 groups: Group A – intravaginal torsion; Group B – extravaginal torsion and Group C – torsion due to long Mesorchium. Results: Of the 50 analyzed testes, 40 (80%) presented bell clapper deformity (with 21 pre-senting intravaginal torsion); 8 testes (16%) had long Mesorchium (4 with torsion), and only 2 (4%) presented normal anatomy in the tunica vaginalis. The most frequently found anatomical relationship between testis and epididymis was Type I- 38 cases (76%); Type II relationship was found in 6 cases (12%) and Type III relationship was found in 6 cases (12%). Conclusions: Intravaginal torsion is the most frequent type, and torsion due to long mesor-chium is associated with cryptorchism. The most frequently found anatomical relation between testis and epididymis in the study group was Type I. Key words: testis; epididymis; spermatic cord torsion; cryptorchidism Int Braz J Urol. 2004; 30: 420-
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anatomic aspects of epididymis and tunica vaginalis in patients with testicular torsion
International Braz J Urol, 2004Co-Authors: Luciano A Favorito, Andre G Cavalcante, Waldemar S CostaAbstract:Objective: To analyze the morphology of epididymis and tunica vaginalis as well as their anatomical anomalies in patients with testicular torsion. Materials and Methods: We studied 25 patients (50 testes) aged between 12 and 23 years (mean 15.6). Torsion length ranged from 2 hours to 2 days (mean 8 hours). Epididymal anatomy was classified in 6 groups: Type I - epididymis united to the testis by its head and tail; Type II - epididymis totally united to the testis; Type III - disjunction of epididymal tail; Type IV - disjunction of epididymal head; Type V - total disjunction between testis and epididymis, and Type VI - epididymal atresia. The type of torsion was classified in 3 groups: Group A – intravaginal torsion; Group B – extravaginal torsion and Group C – torsion due to long Mesorchium. Results: Of the 50 analyzed testes, 40 (80%) presented bell clapper deformity (with 21 presenting intravaginal torsion); 8 testes (16%) had long Mesorchium (4 with torsion), and only 2 (4%) presented normal anatomy in the tunica vaginalis. The most frequently found anatomical relationship between testis and epididymis was Type I - 38 cases (76%); Type II relationship was found in 6 cases (12%) and Type III relationship was found in 6 cases (12%). Conclusions: Intravaginal torsion is the most frequent type, and torsion due to long Mesorchium is associated with cryptorchism. The most frequently found anatomical relation between testis and epididymis in the study group was Type I.