The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Mitsuyoshi Ueda - One of the best experts on this subject based on the ideXlab platform.
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Molecular design of the microbial cell surface toward the recovery of Metal Ions
Current Opinion in Biotechnology, 2011Co-Authors: Kouichi Kuroda, Mitsuyoshi UedaAbstract:The genetic engineering of microorganisms to adsorb Metal Ions is an attractive method to facilitate the environmental cleanup of Metal pollution and to enrich the recovery of Metal Ions such as rare Metal Ions. For the recovery of Metal Ions by microorganisms, cell surface design is an effective strategy for the molecular breeding of bioadsorbents as an alternative to intracellular accumulation. The cell surface display of known Metal-binding proteins/peptides and the molecular design of novel Metal-binding proteins/peptides have been performed using a cell surface engineering approach. The adsorption of specific Metal Ions is the important challenge for the practical recovery of Metal Ions. In this paper, we discuss the recent progress in surface-engineered bioadsorbents for the recovery of Metal Ions. © 2011 Elsevier Ltd.
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Yeast Biosorption and Recycling of Metal Ions by Cell Surface Engineering
Microbial Biosorption of Metals, 2011Co-Authors: Kouichi Kuroda, Mitsuyoshi UedaAbstract:Metal biosorption by microorganisms contributes to the removal of toxic heavy Metal Ions and collection of Metal resources from waste streams. Cell surface biosorption enhanced by cell surface engineering is a unique and effective approach for the construction of a novel biosorbent. Through biosorption, adsorbed Metal Ions can be easily recovered without disintegration of cells, and the repeated use of cells as adsorbents for the biosorption and recuperation of Metal Ions becomes feasible. Thus, engineering of cell surfaces for enhanced biosorption of Metal Ions has the potential to be more suitable than genetic manipulatIons that promote intracellular accumulation. Metal-binding proteins and peptides were displayed on the yeast cell surface by an α-agglutinin-based display system, and cell surface-engineered yeasts showed enhanced biosorption of, and tolerance to, heavy Metal Ions. In addition, a yeast biosorbent for biosorption and recovery of molybdate Ions was constructed by cell surface display of molybdate-binding protein (ModE). The Metal biosorption ability of cell surface-engineered yeasts relies upon features of the displayed Metal-binding proteins and natural properties of particular cell wall. Metal-binding proteins with a capacity to form selective coordination spheres and provide tailored biosorption could be generated by direct screening a mutant library with combinatorial mutatIons in the Metal recognition domain at the yeast cell wall background. Therefore, generation of novel Metal-binding proteins and molecular breeding of yeast biosorbents showing selective biosorption can be concurrently achieved by cell surface engineering.
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engineering of microorganisms towards recovery of rare Metal Ions
Applied Microbiology and Biotechnology, 2010Co-Authors: Kouichi Kuroda, Mitsuyoshi UedaAbstract:The bioadsorption of Metal Ions using microorganisms is an attractive technology for the recovery of rare Metal Ions as well as removal of toxic heavy Metal Ions from aqueous solution. In initial attempts, microorganisms with the ability to accumulate Metal Ions were isolated from nature and intracellular accumulation was enhanced by the overproduction of Metal-binding proteins in the cytoplasm. As an alternative, the cell surface design of microorganisms by cell surface engineering is an emerging strategy for bioadsorption and recovery of Metal Ions. Cell surface engineering was firstly applied to the construction of a bioadsorbent to adsorb heavy Metal Ions for bioremediation. Cell surface adsorption of Metal Ions is rapid and reversible. Therefore, adsorbed Metal Ions can be easily recovered without cell breakage, and the bioadsorbent can be reused or regenerated. These advantages are suitable for the recovery of rare Metal Ions. Actually, the cell surface display of a molybdate-binding protein on yeast led to the enhanced adsorption of molybdate, one of the rare Metal Ions. An additional advantage is that the cell surface display system allows high-throughput screening of protein/peptide libraries owing to the direct evaluation of the displayed protein/peptide without purification and concentration. Therefore, the creation of novel Metal-binding protein/peptide and engineering of microorganisms towards the recovery of rare Metal Ions could be simultaneously achieved.
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Engineering of microorganisms towards recovery of rare Metal Ions
Applied Microbiology and Biotechnology, 2010Co-Authors: Kouichi Kuroda, Mitsuyoshi UedaAbstract:The bioadsorption of Metal Ions using microorganisms is an attractive technology for the recovery of rare Metal Ions as well as removal of toxic heavy Metal Ions from aqueous solution. In initial attempts, microorganisms with the ability to accumulate Metal Ions were isolated from nature and intracellular accumulation was enhanced by the overproduction of Metal-binding proteins in the cytoplasm. As an alternative, the cell surface design of microorganisms by cell surface engineering is an emerging strategy for bioadsorption and recovery of Metal Ions. Cell surface engineering was firstly applied to the construction of a bioadsorbent to adsorb heavy Metal Ions for bioremediation. Cell surface adsorption of Metal Ions is rapid and reversible. Therefore, adsorbed Metal Ions can be easily recovered without cell breakage, and the bioadsorbent can be reused or regenerated. These advantages are suitable for the recovery of rare Metal Ions. Actually, the cell surface display of a molybdate-binding protein on yeast led to the enhanced adsorption of molybdate, one of the rare Metal Ions. An additional advantage is that the cell surface display system allows high-throughput screening of protein/peptide libraries owing to the direct evaluation of the displayed protein/peptide without purification and concentration. Therefore, the creation of novel Metal-binding protein/peptide and engineering of microorganisms towards the recovery of rare Metal Ions could be simultaneously achieved. © 2010 Springer-Verlag.
Kouichi Kuroda - One of the best experts on this subject based on the ideXlab platform.
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Recovery of Rare Metal Ions
Yeast Cell Surface Engineering, 2019Co-Authors: Kouichi KurodaAbstract:Metals are essential materials that play an important role in several processes of life. Some Metals are necessary as trace nutrients but can be toxic at excessively high levels. Other Metals are nonessential and toxic even at trace amounts. Along with the development of the high-tech industry in recent years, the use of Metals, such as rare Metals having various useful characteristics, has been promoted. Rare Metals are also called industrial vitamins and are indispensable for various high-tech products. Therefore, their importance has been increasing in recent years. Cell surface adsorption of Metal Ions can be used not only for removing toxic Metal Ions but also for recovering useful and rare Metal Ions. By displaying the proteins that can bind to rare Metal Ions on the cell surface, it is possible to construct surface-engineered yeasts that can adsorb rare Metal Ions such as molybdate or uranyl Ions. It is important to possess the ability to selectively recover target Metal Ions from complex ion mixtures, which can be achieved using protein engineering. For example, the introduction of an amino acid mutation into the molybdate-binding protein (ModE) displayed on the yeast cell surface has allowed for the selective adsorption of tungstate Ions. Creation of novel Metal-binding proteins/peptides with the ability to selectively sequester target rare Metal Ions is crucial for further development of the technology required to improve our capability of recovering rare Metal Ions from the aquatic environment using genetically engineered microorganisms.
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Molecular design of the microbial cell surface toward the recovery of Metal Ions
Current Opinion in Biotechnology, 2011Co-Authors: Kouichi Kuroda, Mitsuyoshi UedaAbstract:The genetic engineering of microorganisms to adsorb Metal Ions is an attractive method to facilitate the environmental cleanup of Metal pollution and to enrich the recovery of Metal Ions such as rare Metal Ions. For the recovery of Metal Ions by microorganisms, cell surface design is an effective strategy for the molecular breeding of bioadsorbents as an alternative to intracellular accumulation. The cell surface display of known Metal-binding proteins/peptides and the molecular design of novel Metal-binding proteins/peptides have been performed using a cell surface engineering approach. The adsorption of specific Metal Ions is the important challenge for the practical recovery of Metal Ions. In this paper, we discuss the recent progress in surface-engineered bioadsorbents for the recovery of Metal Ions. © 2011 Elsevier Ltd.
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Yeast Biosorption and Recycling of Metal Ions by Cell Surface Engineering
Microbial Biosorption of Metals, 2011Co-Authors: Kouichi Kuroda, Mitsuyoshi UedaAbstract:Metal biosorption by microorganisms contributes to the removal of toxic heavy Metal Ions and collection of Metal resources from waste streams. Cell surface biosorption enhanced by cell surface engineering is a unique and effective approach for the construction of a novel biosorbent. Through biosorption, adsorbed Metal Ions can be easily recovered without disintegration of cells, and the repeated use of cells as adsorbents for the biosorption and recuperation of Metal Ions becomes feasible. Thus, engineering of cell surfaces for enhanced biosorption of Metal Ions has the potential to be more suitable than genetic manipulatIons that promote intracellular accumulation. Metal-binding proteins and peptides were displayed on the yeast cell surface by an α-agglutinin-based display system, and cell surface-engineered yeasts showed enhanced biosorption of, and tolerance to, heavy Metal Ions. In addition, a yeast biosorbent for biosorption and recovery of molybdate Ions was constructed by cell surface display of molybdate-binding protein (ModE). The Metal biosorption ability of cell surface-engineered yeasts relies upon features of the displayed Metal-binding proteins and natural properties of particular cell wall. Metal-binding proteins with a capacity to form selective coordination spheres and provide tailored biosorption could be generated by direct screening a mutant library with combinatorial mutatIons in the Metal recognition domain at the yeast cell wall background. Therefore, generation of novel Metal-binding proteins and molecular breeding of yeast biosorbents showing selective biosorption can be concurrently achieved by cell surface engineering.
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engineering of microorganisms towards recovery of rare Metal Ions
Applied Microbiology and Biotechnology, 2010Co-Authors: Kouichi Kuroda, Mitsuyoshi UedaAbstract:The bioadsorption of Metal Ions using microorganisms is an attractive technology for the recovery of rare Metal Ions as well as removal of toxic heavy Metal Ions from aqueous solution. In initial attempts, microorganisms with the ability to accumulate Metal Ions were isolated from nature and intracellular accumulation was enhanced by the overproduction of Metal-binding proteins in the cytoplasm. As an alternative, the cell surface design of microorganisms by cell surface engineering is an emerging strategy for bioadsorption and recovery of Metal Ions. Cell surface engineering was firstly applied to the construction of a bioadsorbent to adsorb heavy Metal Ions for bioremediation. Cell surface adsorption of Metal Ions is rapid and reversible. Therefore, adsorbed Metal Ions can be easily recovered without cell breakage, and the bioadsorbent can be reused or regenerated. These advantages are suitable for the recovery of rare Metal Ions. Actually, the cell surface display of a molybdate-binding protein on yeast led to the enhanced adsorption of molybdate, one of the rare Metal Ions. An additional advantage is that the cell surface display system allows high-throughput screening of protein/peptide libraries owing to the direct evaluation of the displayed protein/peptide without purification and concentration. Therefore, the creation of novel Metal-binding protein/peptide and engineering of microorganisms towards the recovery of rare Metal Ions could be simultaneously achieved.
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Engineering of microorganisms towards recovery of rare Metal Ions
Applied Microbiology and Biotechnology, 2010Co-Authors: Kouichi Kuroda, Mitsuyoshi UedaAbstract:The bioadsorption of Metal Ions using microorganisms is an attractive technology for the recovery of rare Metal Ions as well as removal of toxic heavy Metal Ions from aqueous solution. In initial attempts, microorganisms with the ability to accumulate Metal Ions were isolated from nature and intracellular accumulation was enhanced by the overproduction of Metal-binding proteins in the cytoplasm. As an alternative, the cell surface design of microorganisms by cell surface engineering is an emerging strategy for bioadsorption and recovery of Metal Ions. Cell surface engineering was firstly applied to the construction of a bioadsorbent to adsorb heavy Metal Ions for bioremediation. Cell surface adsorption of Metal Ions is rapid and reversible. Therefore, adsorbed Metal Ions can be easily recovered without cell breakage, and the bioadsorbent can be reused or regenerated. These advantages are suitable for the recovery of rare Metal Ions. Actually, the cell surface display of a molybdate-binding protein on yeast led to the enhanced adsorption of molybdate, one of the rare Metal Ions. An additional advantage is that the cell surface display system allows high-throughput screening of protein/peptide libraries owing to the direct evaluation of the displayed protein/peptide without purification and concentration. Therefore, the creation of novel Metal-binding protein/peptide and engineering of microorganisms towards the recovery of rare Metal Ions could be simultaneously achieved. © 2010 Springer-Verlag.
Claudia Tomasini - One of the best experts on this subject based on the ideXlab platform.
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Formation of gels in the presence of Metal Ions
Amino Acids, 2011Co-Authors: Nicola Castellucci, Giuseppe Falini, Gaetano Angelici, Claudia TomasiniAbstract:A small library of stereoisomeric pseudopeptides able to make gels in different solvents has been prepared and their attitude to make gels in the presence of several Metal Ions was evaluated. Four benzyl esters and four carboxylic acids, all containing a moiety of azelaic acid (a long chain dicarboxylic acid) coupled with four different pseudopeptide moieties sharing the same skeleton (a phenyl group one atom apart from the oxazolidin-2-one carboxylic group), were synthesized in solution, by standard coupling reaction. The tendency of these pseudopeptides to form gels was evaluated using the inversion test of 10 mM solutIons of pure compounds and of stoichiometric mixtures of pseudopeptides and Metal Ions. To obtain additional information on the molecular association, the gel samples were left dry in the air to form xerogels that were further analyzed using SEM and XRD. The formation of gel containing Zn(II) or Cu(II) Ions gave good results in term of incorporation of the Metal Ions, while the presence of Cu(I), Al(III) and Mg(II) gave less satisfactory results. This outcome is a first insight in the formation of stable LMWGs formed by stoichiometric mixtures of pseudopeptides and Metal Ions. Further studies will be carried out to develop similar compounds of pharmacological interest.
Nicola Castellucci - One of the best experts on this subject based on the ideXlab platform.
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Formation of gels in the presence of Metal Ions
Amino Acids, 2011Co-Authors: Nicola Castellucci, Giuseppe Falini, Gaetano Angelici, Claudia TomasiniAbstract:A small library of stereoisomeric pseudopeptides able to make gels in different solvents has been prepared and their attitude to make gels in the presence of several Metal Ions was evaluated. Four benzyl esters and four carboxylic acids, all containing a moiety of azelaic acid (a long chain dicarboxylic acid) coupled with four different pseudopeptide moieties sharing the same skeleton (a phenyl group one atom apart from the oxazolidin-2-one carboxylic group), were synthesized in solution, by standard coupling reaction. The tendency of these pseudopeptides to form gels was evaluated using the inversion test of 10 mM solutIons of pure compounds and of stoichiometric mixtures of pseudopeptides and Metal Ions. To obtain additional information on the molecular association, the gel samples were left dry in the air to form xerogels that were further analyzed using SEM and XRD. The formation of gel containing Zn(II) or Cu(II) Ions gave good results in term of incorporation of the Metal Ions, while the presence of Cu(I), Al(III) and Mg(II) gave less satisfactory results. This outcome is a first insight in the formation of stable LMWGs formed by stoichiometric mixtures of pseudopeptides and Metal Ions. Further studies will be carried out to develop similar compounds of pharmacological interest.
Marta I Litter - One of the best experts on this subject based on the ideXlab platform.
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heterogeneous photocatalysis transition Metal Ions in photocatalytic systems
Applied Catalysis B-environmental, 1999Co-Authors: Marta I LitterAbstract:Abstract The presence of transition Metal Ions in photocatalytic reactIons is reviewed according to two main approaches: (a) the influence of transition Metal Ions on the rate of photocatalytic reactIons (mainly oxidation) and (b) the transformation of the Ions to less toxic species or their deposition on the semiconductor catalyst surface for recovery of expensive and useful Metals. Most of the proposed mechanisms are discussed, together with experimental physicochemical evidences that support the involved pathways. Practical applicatIons related to environmental protection and industrial processes are described.
