The Experts below are selected from a list of 20205 Experts worldwide ranked by ideXlab platform
Richardson I.m. - One of the best experts on this subject based on the ideXlab platform.
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Numerical study of molten Metal Melt pool behaviour during conduction-mode laser spot Melting
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Molten Metal Melt pools are characterised by highly non-linear responses, which are very sensitive to imposed boundary conditions. Temporal and spatial variations in the energy flux distribution are often neglected in numerical simulations of Melt pool behaviour. Additionally, thermo-physical properties of materials are commonly changed to achieve agreement between predicted Melt-pool shape and experimental post-solidification macrograph. Focusing on laser spot Melting in conduction mode, we investigated the influence of dynamically adjusted energy flux distribution and changing thermo-physical material properties on Melt pool oscillatory behaviour using both deformable and non-deformable assumptions for the gas-Metal interface. Our results demonstrate that adjusting the absorbed energy flux affects the oscillatory fluid flow behaviour in the Melt pool and consequently the predicted Melt-pool shape and size. We also show that changing the thermo-physical material properties artificially or using a non-deformable surface assumption lead to significant differences in Melt pool oscillatory behaviour compared to the cases in which these assumptions are not made.MSE-5ChemE/Transport Phenomen
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Numerical study of molten Metal Melt pool behaviour during conduction-mode laser spot Melting
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Molten Metal Melt pools are characterised by highly non-linear responses, which are very sensitive to imposed boundary conditions. Temporal and spatial variations in the energy flux distribution are often neglected in numerical simulations of Melt pool behaviour. Additionally, thermo-physical properties of materials are commonly changed to achieve agreement between predicted Melt-pool shape and experimental post-solidification macrograph. Focusing on laser spot Melting in conduction mode, we investigated the influence of dynamically adjusted energy flux distribution and changing thermo-physical material properties on Melt pool oscillatory behaviour using both deformable and non-deformable assumptions for the gas-Metal interface. Our results demonstrate that adjusting the absorbed energy flux affects the oscillatory fluid flow behaviour in the Melt pool and consequently the predicted Melt-pool shape and size. We also show that changing the thermo-physical material properties artificially or using a non-deformable surface assumption lead to significant differences in Melt pool oscillatory behaviour compared to the cases in which these assumptions are not made.Team Marcel HermansChemE/Transport Phenomen
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A simulation-based approach to characterise Melt-pool oscillations during gas tungsten arc welding
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Development, optimisation and qualification of welding and additive manufacturing procedures to date have largely been undertaken on an experimental trial and error basis, which imposes significant costs. Numerical simulations are acknowledged as a promising alternative to experiments, and can improve the understanding of the complex process behaviour. In the present work, we propose a simulation-based approach to study and characterise molten Metal Melt pool oscillatory behaviour during arc welding. We implement a high-fidelity three-dimensional model based on the finite-volume method that takes into account the effects of surface deformation on arc power-density and force distributions. These factors are often neglected in numerical simulations of welding and additive manufacturing. Utilising this model, we predict complex molten Metal flow in Melt pools and associated Melt-pool surface oscillations during both steady-current and pulsed-current gas tungsten arc welding (GTAW). An analysis based on a wavelet transform was performed to extract the time-frequency content of the displacement signals obtained from numerical simulations. Our results confirm that the frequency of oscillations for a fully penetrated Melt pool is lower than that of a partially penetrated Melt pool with an abrupt change from partial to full penetration. We find that during transition from partial to full penetration state, two dominant frequencies coexist in the time-frequency spectrum. The results demonstrate that Melt-pool oscillations profoundly depend on Melt-pool shape and convection in the Melt pool, which in turn is influenced by process parameters and material properties. The present numerical simulations reveal the unsteady evolution of Melt pool oscillatory behaviour that are not predictable from published theoretical analyses. Additionally, using the proposed simulation-based approach, the need of triggering the Melt-pool oscillations is expendable since even small surface displacements are detectable, which are not sensible to the current measurement devices employed in experiments.MSE-5ChemE/Transport Phenomen
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A simulation-based approach to characterise Melt-pool oscillations during gas tungsten arc welding
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Development, optimisation and qualification of welding and additive manufacturing procedures to date have largely been undertaken on an experimental trial and error basis, which imposes significant costs. Numerical simulations are acknowledged as a promising alternative to experiments, and can improve the understanding of the complex process behaviour. In the present work, we propose a simulation-based approach to study and characterise molten Metal Melt pool oscillatory behaviour during arc welding. We implement a high-fidelity three-dimensional model based on the finite-volume method that takes into account the effects of surface deformation on arc power-density and force distributions. These factors are often neglected in numerical simulations of welding and additive manufacturing. Utilising this model, we predict complex molten Metal flow in Melt pools and associated Melt-pool surface oscillations during both steady-current and pulsed-current gas tungsten arc welding (GTAW). An analysis based on a wavelet transform was performed to extract the time-frequency content of the displacement signals obtained from numerical simulations. Our results confirm that the frequency of oscillations for a fully penetrated Melt pool is lower than that of a partially penetrated Melt pool with an abrupt change from partial to full penetration. We find that during transition from partial to full penetration state, two dominant frequencies coexist in the time-frequency spectrum. The results demonstrate that Melt-pool oscillations profoundly depend on Melt-pool shape and convection in the Melt pool, which in turn is influenced by process parameters and material properties. The present numerical simulations reveal the unsteady evolution of Melt pool oscillatory behaviour that are not predictable from published theoretical analyses. Additionally, using the proposed simulation-based approach, the need of triggering the Melt-pool oscillations is expendable since even small surface displacements are detectable, which are not sensible to the current measurement devices employed in experiments.
Horst Biermann - One of the best experts on this subject based on the ideXlab platform.
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microstructure of non Metallic inclusions identified in cast steel 42crmo4 after Metal Melt filtration by novel foam filters
2016Co-Authors: Anja Weidner, Christos G. Aneziris, Marcus Emmel, Anne Schmidt, Dominik Krewerth, Birgit Witschel, Johannes Gleinig, Olena Volkova, Horst BiermannAbstract:Non-Metallic inclusions can affect significantly the mechanical properties of Metallic materials. Mainly for safety relevant components, it is important to reduce size and number of non-Metallic inclusions. Recent research work is focused on the development of new reactive, active, and functionalized filters for the reduction of non-Metallic inclusions. A detailed knowledge on the genesis of inclusions is essential to improve the entrapment capability. The paper is focused on the microstructure of inclusions formed in cast steel 42CrMo4 after the application of two filter variants. Both, inclusions on the filter surface as well as inclusions in the as-cast steel are investigated using scanning electron microscopy. Their chemical composition and their crystal structure are identified by combined EBSD and EDS measurements. The three-dimensional morphology of the inclusions is analyzed by deep etching technique as well. Finally, the clusters and agglomerates exhibit a quite complex microstructure. Thus, the majority of inclusion clusters consists of alumina, spinel, mullite, MnS, and TiOx. This agrees well to the scenario of the evolution of inclusions described in the literature.
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influence of non Metallic inclusions on fatigue life in the very high cycle fatigue regime
2016Co-Authors: Dominik Krewerth, Anja Weidner, T Lippmann, Horst BiermannAbstract:Abstract The present paper deals with the influence of non-Metallic inclusions on fatigue life in the high cycle fatigue and the very high cycle fatigue regime. For that purpose, several castings of steel 42CrMo4 (AISI 4140, DIN EN 1.7225) were produced by using recently developed novel Metal-Melt filters. The specimens were tested in hot-isostatically pressed and heat treated condition. After fatigue failure every fracture surface was intensively investigated by scanning electron microscopy in order to define the type, the size, the chemical composition, the morphology and the location of the crack initiating discontinuity. Subsequently, Murakami’s √ area model was used for the evaluation of the influence of non-Metallic inclusions on the fatigue life. In the present investigation four common types of chemical compositions of crack initiating discontinuities were identified. Furthermore, four different internal failure types and their influence on the fatigue life in cast steel were investigated and described. Thus, the present contribution proposes a basic correlation determined from fatigue lives in case of various internal crack initiation types. The key parameters for fatigue life prediction in case of internal fatigue failure in the very high cycle fatigue regime are (i) the size of the crack initiating discontinuity, (ii) the inclusion depth and (iii) the crack initiating failure type.
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application of full surface view in situ thermography measurements during ultrasonic fatigue of cast steel g42crmo4
2015Co-Authors: Dominik Krewerth, Anja Weidner, T Lippmann, Horst BiermannAbstract:Abstract In the present investigation a full-surface view in situ thermography method is adapted to an ultrasonic fatigue testing system. Full-surface view in situ thermography measurements were successfully performed in the high cycle fatigue and in the very high cycle fatigue regime on cast steel G42CrMo4 in the quenched and tempered state. The method enables the monitoring of the entire cylindrical specimen circumference during fatigue testing by infrared temperature field measurements with one thermocamera and two mirrors. Moreover, by correlating fractography and thermography the precise determination of the location of the crack initiation site and the time of final crack growth is possible. The technique is applied to study crack initiation at non-Metallic inclusions in the investigated cast steel specimens. Moreover, the effect of a novel carbon-bonded Metal Melt filter coated with a functionalized spinel (MgAl 2 O 4 ) coating is evaluated by ultrasonic fatigue testing in combination with the full-surface view in situ thermography technique and subsequent scanning electron microscopy.
Ebrahimi Amin - One of the best experts on this subject based on the ideXlab platform.
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Numerical study of molten Metal Melt pool behaviour during conduction-mode laser spot Melting
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Molten Metal Melt pools are characterised by highly non-linear responses, which are very sensitive to imposed boundary conditions. Temporal and spatial variations in the energy flux distribution are often neglected in numerical simulations of Melt pool behaviour. Additionally, thermo-physical properties of materials are commonly changed to achieve agreement between predicted Melt-pool shape and experimental post-solidification macrograph. Focusing on laser spot Melting in conduction mode, we investigated the influence of dynamically adjusted energy flux distribution and changing thermo-physical material properties on Melt pool oscillatory behaviour using both deformable and non-deformable assumptions for the gas-Metal interface. Our results demonstrate that adjusting the absorbed energy flux affects the oscillatory fluid flow behaviour in the Melt pool and consequently the predicted Melt-pool shape and size. We also show that changing the thermo-physical material properties artificially or using a non-deformable surface assumption lead to significant differences in Melt pool oscillatory behaviour compared to the cases in which these assumptions are not made.MSE-5ChemE/Transport Phenomen
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Numerical study of molten Metal Melt pool behaviour during conduction-mode laser spot Melting
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Molten Metal Melt pools are characterised by highly non-linear responses, which are very sensitive to imposed boundary conditions. Temporal and spatial variations in the energy flux distribution are often neglected in numerical simulations of Melt pool behaviour. Additionally, thermo-physical properties of materials are commonly changed to achieve agreement between predicted Melt-pool shape and experimental post-solidification macrograph. Focusing on laser spot Melting in conduction mode, we investigated the influence of dynamically adjusted energy flux distribution and changing thermo-physical material properties on Melt pool oscillatory behaviour using both deformable and non-deformable assumptions for the gas-Metal interface. Our results demonstrate that adjusting the absorbed energy flux affects the oscillatory fluid flow behaviour in the Melt pool and consequently the predicted Melt-pool shape and size. We also show that changing the thermo-physical material properties artificially or using a non-deformable surface assumption lead to significant differences in Melt pool oscillatory behaviour compared to the cases in which these assumptions are not made.Team Marcel HermansChemE/Transport Phenomen
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A simulation-based approach to characterise Melt-pool oscillations during gas tungsten arc welding
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Development, optimisation and qualification of welding and additive manufacturing procedures to date have largely been undertaken on an experimental trial and error basis, which imposes significant costs. Numerical simulations are acknowledged as a promising alternative to experiments, and can improve the understanding of the complex process behaviour. In the present work, we propose a simulation-based approach to study and characterise molten Metal Melt pool oscillatory behaviour during arc welding. We implement a high-fidelity three-dimensional model based on the finite-volume method that takes into account the effects of surface deformation on arc power-density and force distributions. These factors are often neglected in numerical simulations of welding and additive manufacturing. Utilising this model, we predict complex molten Metal flow in Melt pools and associated Melt-pool surface oscillations during both steady-current and pulsed-current gas tungsten arc welding (GTAW). An analysis based on a wavelet transform was performed to extract the time-frequency content of the displacement signals obtained from numerical simulations. Our results confirm that the frequency of oscillations for a fully penetrated Melt pool is lower than that of a partially penetrated Melt pool with an abrupt change from partial to full penetration. We find that during transition from partial to full penetration state, two dominant frequencies coexist in the time-frequency spectrum. The results demonstrate that Melt-pool oscillations profoundly depend on Melt-pool shape and convection in the Melt pool, which in turn is influenced by process parameters and material properties. The present numerical simulations reveal the unsteady evolution of Melt pool oscillatory behaviour that are not predictable from published theoretical analyses. Additionally, using the proposed simulation-based approach, the need of triggering the Melt-pool oscillations is expendable since even small surface displacements are detectable, which are not sensible to the current measurement devices employed in experiments.MSE-5ChemE/Transport Phenomen
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A simulation-based approach to characterise Melt-pool oscillations during gas tungsten arc welding
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Development, optimisation and qualification of welding and additive manufacturing procedures to date have largely been undertaken on an experimental trial and error basis, which imposes significant costs. Numerical simulations are acknowledged as a promising alternative to experiments, and can improve the understanding of the complex process behaviour. In the present work, we propose a simulation-based approach to study and characterise molten Metal Melt pool oscillatory behaviour during arc welding. We implement a high-fidelity three-dimensional model based on the finite-volume method that takes into account the effects of surface deformation on arc power-density and force distributions. These factors are often neglected in numerical simulations of welding and additive manufacturing. Utilising this model, we predict complex molten Metal flow in Melt pools and associated Melt-pool surface oscillations during both steady-current and pulsed-current gas tungsten arc welding (GTAW). An analysis based on a wavelet transform was performed to extract the time-frequency content of the displacement signals obtained from numerical simulations. Our results confirm that the frequency of oscillations for a fully penetrated Melt pool is lower than that of a partially penetrated Melt pool with an abrupt change from partial to full penetration. We find that during transition from partial to full penetration state, two dominant frequencies coexist in the time-frequency spectrum. The results demonstrate that Melt-pool oscillations profoundly depend on Melt-pool shape and convection in the Melt pool, which in turn is influenced by process parameters and material properties. The present numerical simulations reveal the unsteady evolution of Melt pool oscillatory behaviour that are not predictable from published theoretical analyses. Additionally, using the proposed simulation-based approach, the need of triggering the Melt-pool oscillations is expendable since even small surface displacements are detectable, which are not sensible to the current measurement devices employed in experiments.
Kleijn C.r. - One of the best experts on this subject based on the ideXlab platform.
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Numerical study of molten Metal Melt pool behaviour during conduction-mode laser spot Melting
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Molten Metal Melt pools are characterised by highly non-linear responses, which are very sensitive to imposed boundary conditions. Temporal and spatial variations in the energy flux distribution are often neglected in numerical simulations of Melt pool behaviour. Additionally, thermo-physical properties of materials are commonly changed to achieve agreement between predicted Melt-pool shape and experimental post-solidification macrograph. Focusing on laser spot Melting in conduction mode, we investigated the influence of dynamically adjusted energy flux distribution and changing thermo-physical material properties on Melt pool oscillatory behaviour using both deformable and non-deformable assumptions for the gas-Metal interface. Our results demonstrate that adjusting the absorbed energy flux affects the oscillatory fluid flow behaviour in the Melt pool and consequently the predicted Melt-pool shape and size. We also show that changing the thermo-physical material properties artificially or using a non-deformable surface assumption lead to significant differences in Melt pool oscillatory behaviour compared to the cases in which these assumptions are not made.MSE-5ChemE/Transport Phenomen
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Numerical study of molten Metal Melt pool behaviour during conduction-mode laser spot Melting
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Molten Metal Melt pools are characterised by highly non-linear responses, which are very sensitive to imposed boundary conditions. Temporal and spatial variations in the energy flux distribution are often neglected in numerical simulations of Melt pool behaviour. Additionally, thermo-physical properties of materials are commonly changed to achieve agreement between predicted Melt-pool shape and experimental post-solidification macrograph. Focusing on laser spot Melting in conduction mode, we investigated the influence of dynamically adjusted energy flux distribution and changing thermo-physical material properties on Melt pool oscillatory behaviour using both deformable and non-deformable assumptions for the gas-Metal interface. Our results demonstrate that adjusting the absorbed energy flux affects the oscillatory fluid flow behaviour in the Melt pool and consequently the predicted Melt-pool shape and size. We also show that changing the thermo-physical material properties artificially or using a non-deformable surface assumption lead to significant differences in Melt pool oscillatory behaviour compared to the cases in which these assumptions are not made.Team Marcel HermansChemE/Transport Phenomen
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A simulation-based approach to characterise Melt-pool oscillations during gas tungsten arc welding
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Development, optimisation and qualification of welding and additive manufacturing procedures to date have largely been undertaken on an experimental trial and error basis, which imposes significant costs. Numerical simulations are acknowledged as a promising alternative to experiments, and can improve the understanding of the complex process behaviour. In the present work, we propose a simulation-based approach to study and characterise molten Metal Melt pool oscillatory behaviour during arc welding. We implement a high-fidelity three-dimensional model based on the finite-volume method that takes into account the effects of surface deformation on arc power-density and force distributions. These factors are often neglected in numerical simulations of welding and additive manufacturing. Utilising this model, we predict complex molten Metal flow in Melt pools and associated Melt-pool surface oscillations during both steady-current and pulsed-current gas tungsten arc welding (GTAW). An analysis based on a wavelet transform was performed to extract the time-frequency content of the displacement signals obtained from numerical simulations. Our results confirm that the frequency of oscillations for a fully penetrated Melt pool is lower than that of a partially penetrated Melt pool with an abrupt change from partial to full penetration. We find that during transition from partial to full penetration state, two dominant frequencies coexist in the time-frequency spectrum. The results demonstrate that Melt-pool oscillations profoundly depend on Melt-pool shape and convection in the Melt pool, which in turn is influenced by process parameters and material properties. The present numerical simulations reveal the unsteady evolution of Melt pool oscillatory behaviour that are not predictable from published theoretical analyses. Additionally, using the proposed simulation-based approach, the need of triggering the Melt-pool oscillations is expendable since even small surface displacements are detectable, which are not sensible to the current measurement devices employed in experiments.MSE-5ChemE/Transport Phenomen
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A simulation-based approach to characterise Melt-pool oscillations during gas tungsten arc welding
2021Co-Authors: Ebrahimi Amin, Kleijn C.r., Richardson I.m.Abstract:Development, optimisation and qualification of welding and additive manufacturing procedures to date have largely been undertaken on an experimental trial and error basis, which imposes significant costs. Numerical simulations are acknowledged as a promising alternative to experiments, and can improve the understanding of the complex process behaviour. In the present work, we propose a simulation-based approach to study and characterise molten Metal Melt pool oscillatory behaviour during arc welding. We implement a high-fidelity three-dimensional model based on the finite-volume method that takes into account the effects of surface deformation on arc power-density and force distributions. These factors are often neglected in numerical simulations of welding and additive manufacturing. Utilising this model, we predict complex molten Metal flow in Melt pools and associated Melt-pool surface oscillations during both steady-current and pulsed-current gas tungsten arc welding (GTAW). An analysis based on a wavelet transform was performed to extract the time-frequency content of the displacement signals obtained from numerical simulations. Our results confirm that the frequency of oscillations for a fully penetrated Melt pool is lower than that of a partially penetrated Melt pool with an abrupt change from partial to full penetration. We find that during transition from partial to full penetration state, two dominant frequencies coexist in the time-frequency spectrum. The results demonstrate that Melt-pool oscillations profoundly depend on Melt-pool shape and convection in the Melt pool, which in turn is influenced by process parameters and material properties. The present numerical simulations reveal the unsteady evolution of Melt pool oscillatory behaviour that are not predictable from published theoretical analyses. Additionally, using the proposed simulation-based approach, the need of triggering the Melt-pool oscillations is expendable since even small surface displacements are detectable, which are not sensible to the current measurement devices employed in experiments.
Dominik Krewerth - One of the best experts on this subject based on the ideXlab platform.
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microstructure of non Metallic inclusions identified in cast steel 42crmo4 after Metal Melt filtration by novel foam filters
2016Co-Authors: Anja Weidner, Christos G. Aneziris, Marcus Emmel, Anne Schmidt, Dominik Krewerth, Birgit Witschel, Johannes Gleinig, Olena Volkova, Horst BiermannAbstract:Non-Metallic inclusions can affect significantly the mechanical properties of Metallic materials. Mainly for safety relevant components, it is important to reduce size and number of non-Metallic inclusions. Recent research work is focused on the development of new reactive, active, and functionalized filters for the reduction of non-Metallic inclusions. A detailed knowledge on the genesis of inclusions is essential to improve the entrapment capability. The paper is focused on the microstructure of inclusions formed in cast steel 42CrMo4 after the application of two filter variants. Both, inclusions on the filter surface as well as inclusions in the as-cast steel are investigated using scanning electron microscopy. Their chemical composition and their crystal structure are identified by combined EBSD and EDS measurements. The three-dimensional morphology of the inclusions is analyzed by deep etching technique as well. Finally, the clusters and agglomerates exhibit a quite complex microstructure. Thus, the majority of inclusion clusters consists of alumina, spinel, mullite, MnS, and TiOx. This agrees well to the scenario of the evolution of inclusions described in the literature.
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influence of non Metallic inclusions on fatigue life in the very high cycle fatigue regime
2016Co-Authors: Dominik Krewerth, Anja Weidner, T Lippmann, Horst BiermannAbstract:Abstract The present paper deals with the influence of non-Metallic inclusions on fatigue life in the high cycle fatigue and the very high cycle fatigue regime. For that purpose, several castings of steel 42CrMo4 (AISI 4140, DIN EN 1.7225) were produced by using recently developed novel Metal-Melt filters. The specimens were tested in hot-isostatically pressed and heat treated condition. After fatigue failure every fracture surface was intensively investigated by scanning electron microscopy in order to define the type, the size, the chemical composition, the morphology and the location of the crack initiating discontinuity. Subsequently, Murakami’s √ area model was used for the evaluation of the influence of non-Metallic inclusions on the fatigue life. In the present investigation four common types of chemical compositions of crack initiating discontinuities were identified. Furthermore, four different internal failure types and their influence on the fatigue life in cast steel were investigated and described. Thus, the present contribution proposes a basic correlation determined from fatigue lives in case of various internal crack initiation types. The key parameters for fatigue life prediction in case of internal fatigue failure in the very high cycle fatigue regime are (i) the size of the crack initiating discontinuity, (ii) the inclusion depth and (iii) the crack initiating failure type.
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application of full surface view in situ thermography measurements during ultrasonic fatigue of cast steel g42crmo4
2015Co-Authors: Dominik Krewerth, Anja Weidner, T Lippmann, Horst BiermannAbstract:Abstract In the present investigation a full-surface view in situ thermography method is adapted to an ultrasonic fatigue testing system. Full-surface view in situ thermography measurements were successfully performed in the high cycle fatigue and in the very high cycle fatigue regime on cast steel G42CrMo4 in the quenched and tempered state. The method enables the monitoring of the entire cylindrical specimen circumference during fatigue testing by infrared temperature field measurements with one thermocamera and two mirrors. Moreover, by correlating fractography and thermography the precise determination of the location of the crack initiation site and the time of final crack growth is possible. The technique is applied to study crack initiation at non-Metallic inclusions in the investigated cast steel specimens. Moreover, the effect of a novel carbon-bonded Metal Melt filter coated with a functionalized spinel (MgAl 2 O 4 ) coating is evaluated by ultrasonic fatigue testing in combination with the full-surface view in situ thermography technique and subsequent scanning electron microscopy.