The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Mei Zhang - One of the best experts on this subject based on the ideXlab platform.

  • genome wide identification structure characterization expression pattern profiling and substrate specificity of the Metal Tolerance protein family in canavalia rosea sw dc
    Plants (Basel Switzerland), 2021
    Co-Authors: Tao Zou, Ruoyi Lin, Qiming Mei, Zhengfeng Wang, Shuguang Jian, Mei Zhang
    Abstract:

    Plant Metal Tolerance proteins (MTPs) play key roles in heavy Metal absorption and homeostasis in plants. By using genome-wide and phylogenetic approaches, the origin and diversification of MTPs from Canavalia rosea (Sw.) DC. was explored. Canavalia rosea (bay bean) is an extremophile halophyte with strong adaptability to seawater and drought and thereby shows specific Metal Tolerance with a potential phytoremediation ability. However, MTP genes in leguminous plants remain poorly understood. In our study, a total of 12 MTP genes were identified in C. rosea. Multiple sequence alignments showed that all CrMTP proteins possessed the conserved transmembrane domains (TM1 to TM6) and could be classified into three subfamilies: Zn-CDFs (five members), Fe/Zn-CDFs (five members), and Mn-CDFs (two members). Promoter cis-acting element analyses revealed that a distinct number and composition of heavy Metal regulated elements and other stress-responsive elements existed in different promoter regions of CrMTPs. Analysis of transcriptome data revealed organ-specific expression of CrMTP genes and the involvement of this family in heavy Metal stress responses and adaptation of C. rosea to extreme coral reef environments. Furthermore, the Metal-specific activity of several functionally unknown CrMTPs was investigated in yeast. These results will contribute to uncovering the potential functions and molecular mechanisms of heavy Metal absorption, translocation, and accumulation in C. rosea plants.

  • identification of a rice Metal Tolerance protein osmtp11 as a manganese transporter
    PLOS ONE, 2017
    Co-Authors: Mei Zhang, Baoxiu Liu
    Abstract:

    Metal Tolerance proteins (MTPs) are a gene family of cation efflux transporters that occur widely in plants and might serve an essential role in Metal homeostasis and Tolerance. Our research describes the identification, characterization, and localization of OsMTP11, a member of the MTP family from rice. OsMTP11 was expressed constitutively and universally in different tissues in rice plant. Heterologous expression in yeast showed that OsMTP11 complemented the hypersensitivity of mutant strains to Mn, and also complemented yeast mutants to other Metals, including Co and Ni. Real time RT-PCR analysis demonstrated OsMTP11 expression was substantially enhanced following 4 h under Cd, Zn, Ni, and Mn treatments, suggesting possible roles of OsMTP11 involvement in heavy Metal stress responses. Promoter analysis by transgenic assays with GUS as a reporter gene and mRNA in situ hybridization experiments showed that OsMTP11 was expressed specifically in conducting tissues in rice. DNA methylation assays of genomic DNA in rice treated with Cd, Zn, Ni, and Mn revealed that decreased DNA methylation levels were present in the OsMTP11 promoter region, which was consistent with OsMTP11 induced-expression patterns resulting from heavy Metal stress. This result suggested that DNA methylation is one of major factors regulating expression of OsMTP11 through epigenetic mechanisms. OsMTP11 fused to green fluorescent protein (GFP) localized to the entire onion epidermal cell cytoplasm, while vacuolar membrane exhibited increased GFP signals, consistent with an OsMTP11 function in cation sequestration. Our results indicated that OsMTP11 might play vital roles in Mn and other heavy Metal transportation in rice.

  • molecular characterization of a rice Metal Tolerance protein osmtp1
    Plant Cell Reports, 2012
    Co-Authors: Lianyu Yuan, Songguang Yang, Baoxiu Liu, Mei Zhang
    Abstract:

    Rice (Oryza sativa L. ‘Nipponbare’) cDNA subtractive suppression hybridization (SSH) libraries constructed using cadmium (Cd)-treated seedling roots were screened to isolate Cd-responsive genes. A cDNA clone, encoding the rice homolog of Metal Tolerance Protein (OsMTP1), was induced by Cd treatment. Plant MTPs belong to cation diffusion facilitator (CDF) protein family, which are widespread in bacteria, fungi, plants, and animals. OsMTP1 heterologous expression in yeast mutants showed that OsMTP1 was able to complement the mutant strains’ hypersensitivity to Ni, Cd, and Zn, but not other Metals including Co and Mn. OsMTP1 expression increased Tolerance to Zn, Cd, and Ni in wild-type yeast BY4741 during the exponential growth phase. OsMTP1 fused to green fluorescent protein was localized in onion epidermal cell plasma membranes, consistent with an OsMTP1 function in heavy Metal transporting. OsMTP1 dsRNAi mediated by transgenic assay in rice seedlings resulted in heavy Metal sensitivity and changed the heavy Metal accumulation in different organs of mature rice under low-concentration heavy Metal stress. Taken together, our results show that OsMTP1 is a bivalent cation transporter localized in the cell membrane, which is necessary for efficient translocation of Zn, Cd and other heavy Metals, and maintain ion homeostasis in plant.

Baoxiu Liu - One of the best experts on this subject based on the ideXlab platform.

  • identification of a rice Metal Tolerance protein osmtp11 as a manganese transporter
    PLOS ONE, 2017
    Co-Authors: Mei Zhang, Baoxiu Liu
    Abstract:

    Metal Tolerance proteins (MTPs) are a gene family of cation efflux transporters that occur widely in plants and might serve an essential role in Metal homeostasis and Tolerance. Our research describes the identification, characterization, and localization of OsMTP11, a member of the MTP family from rice. OsMTP11 was expressed constitutively and universally in different tissues in rice plant. Heterologous expression in yeast showed that OsMTP11 complemented the hypersensitivity of mutant strains to Mn, and also complemented yeast mutants to other Metals, including Co and Ni. Real time RT-PCR analysis demonstrated OsMTP11 expression was substantially enhanced following 4 h under Cd, Zn, Ni, and Mn treatments, suggesting possible roles of OsMTP11 involvement in heavy Metal stress responses. Promoter analysis by transgenic assays with GUS as a reporter gene and mRNA in situ hybridization experiments showed that OsMTP11 was expressed specifically in conducting tissues in rice. DNA methylation assays of genomic DNA in rice treated with Cd, Zn, Ni, and Mn revealed that decreased DNA methylation levels were present in the OsMTP11 promoter region, which was consistent with OsMTP11 induced-expression patterns resulting from heavy Metal stress. This result suggested that DNA methylation is one of major factors regulating expression of OsMTP11 through epigenetic mechanisms. OsMTP11 fused to green fluorescent protein (GFP) localized to the entire onion epidermal cell cytoplasm, while vacuolar membrane exhibited increased GFP signals, consistent with an OsMTP11 function in cation sequestration. Our results indicated that OsMTP11 might play vital roles in Mn and other heavy Metal transportation in rice.

  • molecular characterization of a rice Metal Tolerance protein osmtp1
    Plant Cell Reports, 2012
    Co-Authors: Lianyu Yuan, Songguang Yang, Baoxiu Liu, Mei Zhang
    Abstract:

    Rice (Oryza sativa L. ‘Nipponbare’) cDNA subtractive suppression hybridization (SSH) libraries constructed using cadmium (Cd)-treated seedling roots were screened to isolate Cd-responsive genes. A cDNA clone, encoding the rice homolog of Metal Tolerance Protein (OsMTP1), was induced by Cd treatment. Plant MTPs belong to cation diffusion facilitator (CDF) protein family, which are widespread in bacteria, fungi, plants, and animals. OsMTP1 heterologous expression in yeast mutants showed that OsMTP1 was able to complement the mutant strains’ hypersensitivity to Ni, Cd, and Zn, but not other Metals including Co and Mn. OsMTP1 expression increased Tolerance to Zn, Cd, and Ni in wild-type yeast BY4741 during the exponential growth phase. OsMTP1 fused to green fluorescent protein was localized in onion epidermal cell plasma membranes, consistent with an OsMTP1 function in heavy Metal transporting. OsMTP1 dsRNAi mediated by transgenic assay in rice seedlings resulted in heavy Metal sensitivity and changed the heavy Metal accumulation in different organs of mature rice under low-concentration heavy Metal stress. Taken together, our results show that OsMTP1 is a bivalent cation transporter localized in the cell membrane, which is necessary for efficient translocation of Zn, Cd and other heavy Metals, and maintain ion homeostasis in plant.

Lorraine E Williams - One of the best experts on this subject based on the ideXlab platform.

  • increased Metal Tolerance and bioaccumulation of zinc and cadmium in chlamydomonas reinhardtii expressing a athma4 c terminal domain protein
    Biotechnology and Bioengineering, 2020
    Co-Authors: Aniefon Ibuot, Rachel E Webster, Lorraine E Williams, Jon K Pittman
    Abstract:

    The use of microalgal biomass for Metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of Metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM Cd and 0.3 mM Zn exposure. The transgenic cells showed increased accumulation and internalization of both Metals compared to wild-type. AtHMA4 was expressed either as the full-length (FL) protein or just the C-terminal (CT) tail, which is known to have Metal-binding sites. Similar Cd and Zn Tolerance and accumulation was observed with expression of either the FL protein or CT domain, suggesting that enhanced Metal Tolerance was mainly due to increased Metal binding rather than Metal transport. The effectiveness of the transgenic cells was further examined by immobilization in calcium alginate to generate microalgal beads that could be added to a Metal contaminated solution. Immobilization maintained Metal Tolerance, while AtHMA4-expressing cells in alginate showed a concentration-dependent increase in Metal biosorption that was significantly greater than alginate beads composed of wild-type cells. This demonstrates that expressing AtHMA4 FL or CT has great potential as a strategy for bioremediation using microalgal biomass.

  • increased Metal Tolerance and bioaccumulation of zinc and cadmium in chlamydomonas reinhardtii expressing a athma4 c terminal domain protein
    bioRxiv, 2020
    Co-Authors: Aniefon Ibuot, Rachel E Webster, Lorraine E Williams, Jon K Pittman
    Abstract:

    The use of microalgal biomass for Metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of Metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM Cd and 0.3 mM Zn exposure. The transgenic cells showed increased accumulation and internalisation of both Metals compared to wild type. AtHMA4 was expressed either as the full-length protein or just the C-terminal tail, which is known to have Metal binding sites. Similar Cd and Zn Tolerance and accumulation was observed with expression of either the full-length protein or C-terminal domain, suggesting that enhanced Metal Tolerance was mainly due to increased Metal binding rather than Metal transport. The effectiveness of the transgenic cells was further examined by immobilisation in calcium alginate to generate microalgal beads that could be added to a Metal contaminated solution. Immobilisation maintained Metal Tolerance, while AtHMA4-expressing cells in alginate showed a concentration-dependent increase in Metal biosorption that was significantly greater than alginate beads composed of wild type cells. This demonstrates that expressing AtHMA4 full-length or C-terminus has great potential as a strategy for bioremediation using microalgal biomass.

  • roles of plant Metal Tolerance proteins mtp in Metal storage and potential use in biofortification strategies
    Frontiers in Plant Science, 2013
    Co-Authors: Felipe Klein Ricachenevsky, Lorraine E Williams, Paloma Koprovski Menguer, Raul Antonio Sperotto, Janette Palma Fett
    Abstract:

    Zinc (Zn) is an essential micronutrient for plants, playing catalytic or structural roles in enzymes, transcription factors, ribosomes, and membranes. In humans, Zn deficiency is the second most common mineral nutritional disorder, affecting around 30% of the world's population. People living in poverty usually have diets based on milled cereals, which contain low Zn concentrations. Biofortification of crops is an attractive cost-effective solution for low mineral dietary intake. In order to increase the amounts of bioavailable Zn in crop edible portions, it is necessary to understand how plants take up, distribute, and store Zn within their tissues, as well as to characterize potential candidate genes for biotechnological manipulation. The Metal Tolerance proteins (MTP) were described as Metal efflux transporters from the cytoplasm, transporting mainly Zn2+ but also Mn2+, Fe2+, Cd2+, Co2+, and Ni2+. Substrate specificity appears to be conserved in phylogenetically related proteins. MTPs characterized so far in plants have a role in general Zn homeostasis and Tolerance to Zn excess; in Tolerance to excess Mn and also in the response to iron (Fe) deficiency. More recently, the first MTPs in crop species have been functionally characterized. In Zn hyperaccumulator plants, the MTP1 protein is related to hyperTolerance to elevated Zn concentrations. Here, we review the current knowledge on this protein family, as well as biochemical functions and physiological roles of MTP transporters in Zn hyperaccumulators and non-accumulators. The potential applications of MTP transporters in biofortification efforts are discussed.

Xiaoe Yang - One of the best experts on this subject based on the ideXlab platform.

  • functional analysis of Metal Tolerance proteins isolated from zn cd hyperaccumulating ecotype and non hyperaccumulating ecotype of sedum alfredii hance
    FEBS Letters, 2011
    Co-Authors: Min Zhang, Xiaoe Yang, Takeshi Senoura, Naoko K Nishizawa
    Abstract:

    The Zn/Cd hyperaccumulating ecotype (HE) of Sedum alfredii Hance can accumulate 24- and 28-fold higher leaf and stem Zn concentrations when compared with the non-hyperaccumulating ecotype (NHE) of Sedum. Heterologous expression of a Metal Tolerance protein (MTP1) encoding gene from HE plants (SaMTP1) or the homologous gene from NHE plants (SnMTP1) suppressed Zn2+ hypersensitivity in the Δzrc1 yeast mutant. In plants, SaMTP1 localized to the tonoplast. Furthermore, MTP1 transcript level in the shoot of HE plants was more than 80-fold higher than that of NHE plants. The transcript level of SaMTP1 in shoot was up-regulated 1-fold by Zn2+ while the expression of SnMTP1 was slightly inhibited. These data suggest that SaMTP1 can play an important role in Zn accumulation in HE plants.

  • molecular mechanisms and genetic basis of heavy Metal Tolerance hyperaccumulation in plants
    Journal of Integrative Plant Biology, 2005
    Co-Authors: Xiaoe Yang, Xiaofen Jin, Ying Feng, Ejazul Islam
    Abstract:

    Abstract: Phytoremediation has gained increased attention as a cost-effective method for the remediation of heavy Metal-contaminated sites. Because some plants possess a range of potential mechanisms that may be involved in the detoxification of heavy Metals, they manage to survive under Metal stresses. High Tolerance to heavy Metal toxicity could rely either on reduced uptake or increased plant internal sequestration, which is manifested by an interaction between a genotype and its environment. The growing application of molecular genetic technologies has led to increased understanding of mechanisms of heavy Metal Tolerance/accumulation in plants and, subsequently, many transgenic plants with increased heavy Metal resistance, as well as increased uptake of heavy Metals, have been developed for the purpose of phytoremediation. In the present review, our major objective is to concisely evaluate the progress made so far in understanding the molecular/cellular mechanisms and genetic basis that control the uptake and detoxification of Metals by plants. (Managing editor: Ping HE)

Jon K Pittman - One of the best experts on this subject based on the ideXlab platform.

  • increased Metal Tolerance and bioaccumulation of zinc and cadmium in chlamydomonas reinhardtii expressing a athma4 c terminal domain protein
    Biotechnology and Bioengineering, 2020
    Co-Authors: Aniefon Ibuot, Rachel E Webster, Lorraine E Williams, Jon K Pittman
    Abstract:

    The use of microalgal biomass for Metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of Metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM Cd and 0.3 mM Zn exposure. The transgenic cells showed increased accumulation and internalization of both Metals compared to wild-type. AtHMA4 was expressed either as the full-length (FL) protein or just the C-terminal (CT) tail, which is known to have Metal-binding sites. Similar Cd and Zn Tolerance and accumulation was observed with expression of either the FL protein or CT domain, suggesting that enhanced Metal Tolerance was mainly due to increased Metal binding rather than Metal transport. The effectiveness of the transgenic cells was further examined by immobilization in calcium alginate to generate microalgal beads that could be added to a Metal contaminated solution. Immobilization maintained Metal Tolerance, while AtHMA4-expressing cells in alginate showed a concentration-dependent increase in Metal biosorption that was significantly greater than alginate beads composed of wild-type cells. This demonstrates that expressing AtHMA4 FL or CT has great potential as a strategy for bioremediation using microalgal biomass.

  • increased Metal Tolerance and bioaccumulation of zinc and cadmium in chlamydomonas reinhardtii expressing a athma4 c terminal domain protein
    bioRxiv, 2020
    Co-Authors: Aniefon Ibuot, Rachel E Webster, Lorraine E Williams, Jon K Pittman
    Abstract:

    The use of microalgal biomass for Metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of Metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM Cd and 0.3 mM Zn exposure. The transgenic cells showed increased accumulation and internalisation of both Metals compared to wild type. AtHMA4 was expressed either as the full-length protein or just the C-terminal tail, which is known to have Metal binding sites. Similar Cd and Zn Tolerance and accumulation was observed with expression of either the full-length protein or C-terminal domain, suggesting that enhanced Metal Tolerance was mainly due to increased Metal binding rather than Metal transport. The effectiveness of the transgenic cells was further examined by immobilisation in calcium alginate to generate microalgal beads that could be added to a Metal contaminated solution. Immobilisation maintained Metal Tolerance, while AtHMA4-expressing cells in alginate showed a concentration-dependent increase in Metal biosorption that was significantly greater than alginate beads composed of wild type cells. This demonstrates that expressing AtHMA4 full-length or C-terminus has great potential as a strategy for bioremediation using microalgal biomass.