The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Sara Pauwels - One of the best experts on this subject based on the ideXlab platform.
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maternal intake of Methyl Group donors affects dna Methylation of metabolic genes in infants
Clinical Epigenetics, 2017Co-Authors: Sara Pauwels, Radu Corneliu Duca, Kathleen Freson, Inge Huybrechts, Manosij Ghosh, Bram Bekaert, Sabine A S Langie, Gudrun KoppenAbstract:Maternal nutrition during pregnancy and infant nutrition in the early postnatal period (lactation) are critically involved in the development and health of the newborn infant. The Maternal Nutrition and Offspring’s Epigenome (MANOE) study was set up to assess the effect of maternal Methyl-Group donor intake (choline, betaine, folate, methionine) on infant DNA Methylation. Maternal intake of dietary Methyl-Group donors was assessed using a food-frequency questionnaire (FFQ). Before and during pregnancy, we evaluated maternal Methyl-Group donor intake through diet and supplementation (folic acid) in relation to gene-specific (IGF2 DMR, DNMT1, LEP, RXRA) buccal epithelial cell DNA Methylation in 6 months old infants (n = 114) via pyrosequencing. In the early postnatal period, we determined the effect of maternal choline intake during lactation (in mothers who breast-fed for at least 3 months) on gene-specific buccal DNA Methylation (n = 65). Maternal dietary and supplemental intake of Methyl-Group donors (folate, betaine, folic acid), only in the periconception period, was associated with buccal cell DNA Methylation in genes related to growth (IGF2 DMR), metabolism (RXRA), and appetite control (LEP). A negative association was found between maternal folate and folic acid intake before pregnancy and infant LEP (slope = −1.233, 95% CI −2.342; −0.125, p = 0.0298) and IGF2 DMR Methylation (slope = −0.706, 95% CI −1.242; −0.107, p = 0.0101), respectively. Positive associations were observed for maternal betaine (slope = 0.875, 95% CI 0.118; 1.633, p = 0.0241) and folate (slope = 0.685, 95% CI 0.245; 1.125, p = 0.0027) intake before pregnancy and RXRA Methylation. Buccal DNMT1 Methylation in the infant was negatively associated with maternal Methyl-Group donor intake in the first and second trimester of pregnancy and negatively in the third trimester. We found no clear association between maternal choline intake during lactation and buccal infant DNA Methylation. This study suggests that maternal dietary and supplemental intake of Methyl-Group donors, especially in the periconception period, can influence infant’s buccal DNA Methylation in genes related to metabolism, growth, appetite regulation, and maintenance of DNA Methylation reactions.
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dietary and supplemental maternal Methyl Group donor intake and cord blood dna Methylation
Epigenetics, 2017Co-Authors: Sara Pauwels, Radu Corneliu Duca, Roland Devlieger, Kathleen Freson, Inge Huybrechts, Gudrun Koppen, Manosij Ghosh, Bram Bekaert, Sabine A S Langie, Lode GodderisAbstract:ABSTRACTMaternal nutrition is critically involved in the development and health of the fetus. We evaluated maternal Methyl-Group donor intake through diet (methionine, betaine, choline, folate) and supplementation (folic acid) before and during pregnancy in relation to global DNA Methylation and hydroxyMethylation and gene specific (IGF2 DMR, DNMT1, LEP, RXRA) cord blood Methylation. A total of 115 mother-infant pairs were enrolled in the MAternal Nutrition and Offspring's Epigenome (MANOE) study. The intake of Methyl-Group donors was assessed using a food-frequency questionnaire. LC-MS/MS and pyrosequencing were used to measure global and gene specific Methylation, respectively. Dietary intake of Methyl-Groups before and during pregnancy was associated with changes in LEP, DNMT1, and RXRA cord blood Methylation. Statistically significant higher cord blood LEP Methylation was observed when mothers started folic acid supplementation more than 6 months before conception compared with 3–6 months before conce...
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maternal Methyl Group donor intake and global dna hydroxy Methylation before and during pregnancy
Nutrients, 2016Co-Authors: Sara Pauwels, Radu Corneliu Duca, Roland Devlieger, Kathleen Freson, Danny Straetmans, Erik Van Herck, Inge Huybrechts, Gudrun Koppen, Lode GodderisAbstract:It is still unclear to which extent Methyl-Group intake during pregnancy can affect maternal global DNA (hydroxyl)Methylation. Pregnancy Methylation profiling and its link with Methyl-Group intake in a healthy population could enhance our understanding of the development of pregnancy related disorders. One hundred forty-eight women were enrolled in the MANOE (MAternal Nutrition and Offspring's Epigenome) study. Thiry-four women were enrolled before pregnancy and 116 during the first trimester of pregnancy. Global DNA (hydroxy)Methylation in blood using LC-MS/MS and dietary Methyl-Group intake (methionine, folate, betaine, and choline) using a food-frequency questionnaire were estimated pre-pregnancy, during each trimester, and at delivery. Global DNA (hydroxy)Methylation levels were highest pre-pregnancy and at weeks 18-22 of pregnancy. We observed a positive relation between folic acid and global DNA Methylation (p = 0.04) and hydroxyMethylation (p = 0.04). A high intake of methionine pre-pregnancy and in the first trimester showed lower (hydroxy)Methylation percentage in weeks 11-13 and weeks 18-22, respectively. Choline and betaine intake in the first weeks was negatively associated with hydroxyMethylation. Women with a high intake of these three Methyl Groups in the second and third trimester showed higher hyrdoxyMethylation/Methylation levels in the third trimester. To conclude, a time trend in DNA (hydroxy)Methylation was found and women with higher Methyl-Group intake showed higher Methylation in the third trimester, and not in earlier phases of pregnancy.
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validation of a food frequency questionnaire assessment of Methyl Group donors using estimated diet records and plasma biomarkers the method of triads
International Journal of Food Sciences and Nutrition, 2014Co-Authors: Sara Pauwels, Inge Huybrechts, Gudrun Koppen, Lode Godderis, Ine Dopere, Greet VansantAbstract:In this study, the intake of Methyl-Group donors (methionine, folate, betaine, and choline) among Flemish women of reproductive age (n = 30) assessed by a 7-d estimated diet record (7 d EDR) and food-frequency questionnaire (FFQ) was compared with plasma S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), and SAH:SAM ratio. Pearson correlation coefficients were calculated between each of the dietary methods and the validity coefficient was calculated using the method of triads. Correlations were higher between intake assessed by the FFQ and biomarkers than between 7 d EDR and biomarkers. The validity coefficients of the FFQ, when using SAH as a biomarker, were high (0.86 for methionine to 0.94 for folate), when the SAH:SAM ratio was used as a biomarker the validity coefficients ranged from 0.63 to 1.00. These data indicate that the FFQ is a reliable tool to estimate the intake of the Methyl-Group donors in women of reproductive age.
Ha Vinh Lam Nguyen - One of the best experts on this subject based on the ideXlab platform.
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internal rotation of the acetyl Methyl Group in Methyl alkyl ketones the microwave spectrum of octan 2 one
ChemPhysChem, 2020Co-Authors: Maike Andresen, Damian Schongen, I Kleiner, Martin Schwell, Wolfgang Stahl, Ha Vinh Lam NguyenAbstract:Methyl n-alkyl ketones form a class of molecules with interesting internal dynamics in the gas-phase. They contain two Methyl Groups undergoing internal rotations, the acetyl Methyl Group and the Methyl Group at the end of the alkyl chain. The torsional barrier of the acetyl Methyl Group is of special importance, since it allows for the discrimination of the conformational structures. As part of the series, the microwave spectrum of octan-2-one was recorded in the frequency range from 2 to 40 GHz, revealing two conformers, one with C1 and one with Cs symmetry. The barriers to internal rotation of the acetyl Methyl Group were determined to be 233.340(28) cm-1 and 185.3490(81) cm-1 , respectively, confirming the link between conformations and barrier heights already established for other Methyl alkyl ketones. Extensive comparisons to molecules in the literature were carried out, and a small overview of general trends and rules concerning the acetyl Methyl torsion is given. For the hexyl Methyl Group, the barrier height is 973.17(60) cm-1 for the C1 conformer and 979.62(69) cm-1 for the Cs conformer.
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internal rotation of the acetyl Methyl Group in Methyl alkyl ketones the microwave spectrum of octan 2 one
ChemPhysChem, 2020Co-Authors: Maike Andresen, Damian Schongen, I Kleiner, Martin Schwell, Wolfgang Stahl, Ha Vinh Lam NguyenAbstract:Methyl n -alkyl ketones form a class of molecules with interesting internal dynamics in the gas-phase. They contain two Methyl Groups undergoing internal rotations, the acetyl Methyl Group and the Methyl Group at the end of the alkyl chain. The torsional barrier of the acetyl Methyl Group is of special importance, since it allows for the discrimination of the conformational structure. As part of the series, the microwave spectrum of octan-2-one was recorded in the frequency range from 2 to 40 GHz, revealing two conformers, one with C 1 and one with C s symmetry. The barriers to internal rotation of the acetyl Methyl Group were determined to be 233.340(28) cm -1 and 185.3490(81) cm -1 , respectively, confirming the link between conformation and barrier height already established for other Methyl alkyl ketones. Extensive comparisons to molecules in the literature were carried out, and a small overview of general trends and rules concerning the acetyl Methyl torsion is given. For the hexyl Methyl Group, the barrier height is 973.17(60) cm -1 for the C 1 conformer and 979.62(69) cm -1 for the C s conformer.
Maike Andresen - One of the best experts on this subject based on the ideXlab platform.
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internal rotation of the acetyl Methyl Group in Methyl alkyl ketones the microwave spectrum of octan 2 one
ChemPhysChem, 2020Co-Authors: Maike Andresen, Damian Schongen, I Kleiner, Martin Schwell, Wolfgang Stahl, Ha Vinh Lam NguyenAbstract:Methyl n-alkyl ketones form a class of molecules with interesting internal dynamics in the gas-phase. They contain two Methyl Groups undergoing internal rotations, the acetyl Methyl Group and the Methyl Group at the end of the alkyl chain. The torsional barrier of the acetyl Methyl Group is of special importance, since it allows for the discrimination of the conformational structures. As part of the series, the microwave spectrum of octan-2-one was recorded in the frequency range from 2 to 40 GHz, revealing two conformers, one with C1 and one with Cs symmetry. The barriers to internal rotation of the acetyl Methyl Group were determined to be 233.340(28) cm-1 and 185.3490(81) cm-1 , respectively, confirming the link between conformations and barrier heights already established for other Methyl alkyl ketones. Extensive comparisons to molecules in the literature were carried out, and a small overview of general trends and rules concerning the acetyl Methyl torsion is given. For the hexyl Methyl Group, the barrier height is 973.17(60) cm-1 for the C1 conformer and 979.62(69) cm-1 for the Cs conformer.
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internal rotation of the acetyl Methyl Group in Methyl alkyl ketones the microwave spectrum of octan 2 one
ChemPhysChem, 2020Co-Authors: Maike Andresen, Damian Schongen, I Kleiner, Martin Schwell, Wolfgang Stahl, Ha Vinh Lam NguyenAbstract:Methyl n -alkyl ketones form a class of molecules with interesting internal dynamics in the gas-phase. They contain two Methyl Groups undergoing internal rotations, the acetyl Methyl Group and the Methyl Group at the end of the alkyl chain. The torsional barrier of the acetyl Methyl Group is of special importance, since it allows for the discrimination of the conformational structure. As part of the series, the microwave spectrum of octan-2-one was recorded in the frequency range from 2 to 40 GHz, revealing two conformers, one with C 1 and one with C s symmetry. The barriers to internal rotation of the acetyl Methyl Group were determined to be 233.340(28) cm -1 and 185.3490(81) cm -1 , respectively, confirming the link between conformation and barrier height already established for other Methyl alkyl ketones. Extensive comparisons to molecules in the literature were carried out, and a small overview of general trends and rules concerning the acetyl Methyl torsion is given. For the hexyl Methyl Group, the barrier height is 973.17(60) cm -1 for the C 1 conformer and 979.62(69) cm -1 for the C s conformer.
Gudrun Koppen - One of the best experts on this subject based on the ideXlab platform.
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maternal intake of Methyl Group donors affects dna Methylation of metabolic genes in infants
Clinical Epigenetics, 2017Co-Authors: Sara Pauwels, Radu Corneliu Duca, Kathleen Freson, Inge Huybrechts, Manosij Ghosh, Bram Bekaert, Sabine A S Langie, Gudrun KoppenAbstract:Maternal nutrition during pregnancy and infant nutrition in the early postnatal period (lactation) are critically involved in the development and health of the newborn infant. The Maternal Nutrition and Offspring’s Epigenome (MANOE) study was set up to assess the effect of maternal Methyl-Group donor intake (choline, betaine, folate, methionine) on infant DNA Methylation. Maternal intake of dietary Methyl-Group donors was assessed using a food-frequency questionnaire (FFQ). Before and during pregnancy, we evaluated maternal Methyl-Group donor intake through diet and supplementation (folic acid) in relation to gene-specific (IGF2 DMR, DNMT1, LEP, RXRA) buccal epithelial cell DNA Methylation in 6 months old infants (n = 114) via pyrosequencing. In the early postnatal period, we determined the effect of maternal choline intake during lactation (in mothers who breast-fed for at least 3 months) on gene-specific buccal DNA Methylation (n = 65). Maternal dietary and supplemental intake of Methyl-Group donors (folate, betaine, folic acid), only in the periconception period, was associated with buccal cell DNA Methylation in genes related to growth (IGF2 DMR), metabolism (RXRA), and appetite control (LEP). A negative association was found between maternal folate and folic acid intake before pregnancy and infant LEP (slope = −1.233, 95% CI −2.342; −0.125, p = 0.0298) and IGF2 DMR Methylation (slope = −0.706, 95% CI −1.242; −0.107, p = 0.0101), respectively. Positive associations were observed for maternal betaine (slope = 0.875, 95% CI 0.118; 1.633, p = 0.0241) and folate (slope = 0.685, 95% CI 0.245; 1.125, p = 0.0027) intake before pregnancy and RXRA Methylation. Buccal DNMT1 Methylation in the infant was negatively associated with maternal Methyl-Group donor intake in the first and second trimester of pregnancy and negatively in the third trimester. We found no clear association between maternal choline intake during lactation and buccal infant DNA Methylation. This study suggests that maternal dietary and supplemental intake of Methyl-Group donors, especially in the periconception period, can influence infant’s buccal DNA Methylation in genes related to metabolism, growth, appetite regulation, and maintenance of DNA Methylation reactions.
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dietary and supplemental maternal Methyl Group donor intake and cord blood dna Methylation
Epigenetics, 2017Co-Authors: Sara Pauwels, Radu Corneliu Duca, Roland Devlieger, Kathleen Freson, Inge Huybrechts, Gudrun Koppen, Manosij Ghosh, Bram Bekaert, Sabine A S Langie, Lode GodderisAbstract:ABSTRACTMaternal nutrition is critically involved in the development and health of the fetus. We evaluated maternal Methyl-Group donor intake through diet (methionine, betaine, choline, folate) and supplementation (folic acid) before and during pregnancy in relation to global DNA Methylation and hydroxyMethylation and gene specific (IGF2 DMR, DNMT1, LEP, RXRA) cord blood Methylation. A total of 115 mother-infant pairs were enrolled in the MAternal Nutrition and Offspring's Epigenome (MANOE) study. The intake of Methyl-Group donors was assessed using a food-frequency questionnaire. LC-MS/MS and pyrosequencing were used to measure global and gene specific Methylation, respectively. Dietary intake of Methyl-Groups before and during pregnancy was associated with changes in LEP, DNMT1, and RXRA cord blood Methylation. Statistically significant higher cord blood LEP Methylation was observed when mothers started folic acid supplementation more than 6 months before conception compared with 3–6 months before conce...
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maternal Methyl Group donor intake and global dna hydroxy Methylation before and during pregnancy
Nutrients, 2016Co-Authors: Sara Pauwels, Radu Corneliu Duca, Roland Devlieger, Kathleen Freson, Danny Straetmans, Erik Van Herck, Inge Huybrechts, Gudrun Koppen, Lode GodderisAbstract:It is still unclear to which extent Methyl-Group intake during pregnancy can affect maternal global DNA (hydroxyl)Methylation. Pregnancy Methylation profiling and its link with Methyl-Group intake in a healthy population could enhance our understanding of the development of pregnancy related disorders. One hundred forty-eight women were enrolled in the MANOE (MAternal Nutrition and Offspring's Epigenome) study. Thiry-four women were enrolled before pregnancy and 116 during the first trimester of pregnancy. Global DNA (hydroxy)Methylation in blood using LC-MS/MS and dietary Methyl-Group intake (methionine, folate, betaine, and choline) using a food-frequency questionnaire were estimated pre-pregnancy, during each trimester, and at delivery. Global DNA (hydroxy)Methylation levels were highest pre-pregnancy and at weeks 18-22 of pregnancy. We observed a positive relation between folic acid and global DNA Methylation (p = 0.04) and hydroxyMethylation (p = 0.04). A high intake of methionine pre-pregnancy and in the first trimester showed lower (hydroxy)Methylation percentage in weeks 11-13 and weeks 18-22, respectively. Choline and betaine intake in the first weeks was negatively associated with hydroxyMethylation. Women with a high intake of these three Methyl Groups in the second and third trimester showed higher hyrdoxyMethylation/Methylation levels in the third trimester. To conclude, a time trend in DNA (hydroxy)Methylation was found and women with higher Methyl-Group intake showed higher Methylation in the third trimester, and not in earlier phases of pregnancy.
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validation of a food frequency questionnaire assessment of Methyl Group donors using estimated diet records and plasma biomarkers the method of triads
International Journal of Food Sciences and Nutrition, 2014Co-Authors: Sara Pauwels, Inge Huybrechts, Gudrun Koppen, Lode Godderis, Ine Dopere, Greet VansantAbstract:In this study, the intake of Methyl-Group donors (methionine, folate, betaine, and choline) among Flemish women of reproductive age (n = 30) assessed by a 7-d estimated diet record (7 d EDR) and food-frequency questionnaire (FFQ) was compared with plasma S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), and SAH:SAM ratio. Pearson correlation coefficients were calculated between each of the dietary methods and the validity coefficient was calculated using the method of triads. Correlations were higher between intake assessed by the FFQ and biomarkers than between 7 d EDR and biomarkers. The validity coefficients of the FFQ, when using SAH as a biomarker, were high (0.86 for methionine to 0.94 for folate), when the SAH:SAM ratio was used as a biomarker the validity coefficients ranged from 0.63 to 1.00. These data indicate that the FFQ is a reliable tool to estimate the intake of the Methyl-Group donors in women of reproductive age.
Lode Godderis - One of the best experts on this subject based on the ideXlab platform.
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dietary and supplemental maternal Methyl Group donor intake and cord blood dna Methylation
Epigenetics, 2017Co-Authors: Sara Pauwels, Radu Corneliu Duca, Roland Devlieger, Kathleen Freson, Inge Huybrechts, Gudrun Koppen, Manosij Ghosh, Bram Bekaert, Sabine A S Langie, Lode GodderisAbstract:ABSTRACTMaternal nutrition is critically involved in the development and health of the fetus. We evaluated maternal Methyl-Group donor intake through diet (methionine, betaine, choline, folate) and supplementation (folic acid) before and during pregnancy in relation to global DNA Methylation and hydroxyMethylation and gene specific (IGF2 DMR, DNMT1, LEP, RXRA) cord blood Methylation. A total of 115 mother-infant pairs were enrolled in the MAternal Nutrition and Offspring's Epigenome (MANOE) study. The intake of Methyl-Group donors was assessed using a food-frequency questionnaire. LC-MS/MS and pyrosequencing were used to measure global and gene specific Methylation, respectively. Dietary intake of Methyl-Groups before and during pregnancy was associated with changes in LEP, DNMT1, and RXRA cord blood Methylation. Statistically significant higher cord blood LEP Methylation was observed when mothers started folic acid supplementation more than 6 months before conception compared with 3–6 months before conce...
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maternal Methyl Group donor intake and global dna hydroxy Methylation before and during pregnancy
Nutrients, 2016Co-Authors: Sara Pauwels, Radu Corneliu Duca, Roland Devlieger, Kathleen Freson, Danny Straetmans, Erik Van Herck, Inge Huybrechts, Gudrun Koppen, Lode GodderisAbstract:It is still unclear to which extent Methyl-Group intake during pregnancy can affect maternal global DNA (hydroxyl)Methylation. Pregnancy Methylation profiling and its link with Methyl-Group intake in a healthy population could enhance our understanding of the development of pregnancy related disorders. One hundred forty-eight women were enrolled in the MANOE (MAternal Nutrition and Offspring's Epigenome) study. Thiry-four women were enrolled before pregnancy and 116 during the first trimester of pregnancy. Global DNA (hydroxy)Methylation in blood using LC-MS/MS and dietary Methyl-Group intake (methionine, folate, betaine, and choline) using a food-frequency questionnaire were estimated pre-pregnancy, during each trimester, and at delivery. Global DNA (hydroxy)Methylation levels were highest pre-pregnancy and at weeks 18-22 of pregnancy. We observed a positive relation between folic acid and global DNA Methylation (p = 0.04) and hydroxyMethylation (p = 0.04). A high intake of methionine pre-pregnancy and in the first trimester showed lower (hydroxy)Methylation percentage in weeks 11-13 and weeks 18-22, respectively. Choline and betaine intake in the first weeks was negatively associated with hydroxyMethylation. Women with a high intake of these three Methyl Groups in the second and third trimester showed higher hyrdoxyMethylation/Methylation levels in the third trimester. To conclude, a time trend in DNA (hydroxy)Methylation was found and women with higher Methyl-Group intake showed higher Methylation in the third trimester, and not in earlier phases of pregnancy.
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validation of a food frequency questionnaire assessment of Methyl Group donors using estimated diet records and plasma biomarkers the method of triads
International Journal of Food Sciences and Nutrition, 2014Co-Authors: Sara Pauwels, Inge Huybrechts, Gudrun Koppen, Lode Godderis, Ine Dopere, Greet VansantAbstract:In this study, the intake of Methyl-Group donors (methionine, folate, betaine, and choline) among Flemish women of reproductive age (n = 30) assessed by a 7-d estimated diet record (7 d EDR) and food-frequency questionnaire (FFQ) was compared with plasma S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), and SAH:SAM ratio. Pearson correlation coefficients were calculated between each of the dietary methods and the validity coefficient was calculated using the method of triads. Correlations were higher between intake assessed by the FFQ and biomarkers than between 7 d EDR and biomarkers. The validity coefficients of the FFQ, when using SAH as a biomarker, were high (0.86 for methionine to 0.94 for folate), when the SAH:SAM ratio was used as a biomarker the validity coefficients ranged from 0.63 to 1.00. These data indicate that the FFQ is a reliable tool to estimate the intake of the Methyl-Group donors in women of reproductive age.
