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Denise Crispim Tavares - One of the best experts on this subject based on the ideXlab platform.

  • Effect of the dibenzylbutyrolactone lignan (-)-hinokinin on doxorubicin and Methyl Methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts.
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Flávia Aparecida Resende, Iara Maluf Tomazella, Lilian Cristina Barbosa, Marina Ponce, Ricardo Andrade Furtado, Ana Carolina Pereira, Marcio Luis Andrade E Silva, Jairo Kenupp Bastos, Denise Crispim Tavares
    Abstract:

    Abstract The dibenzylbutyrolactone lignan (−)-hinokinin (HK) was obtained by partial synthesis from (−)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba . In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound's effect on the activity of the clastogens doxorubicin (DXR) and Methyl Methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128 μM. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity.

  • effect of the dibenzylbutyrolactone lignan hinokinin on doxorubicin and Methyl Methanesulfonate clastogenicity in v79 chinese hamster lung fibroblasts
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Flávia Aparecida Resende, Iara Maluf Tomazella, Lilian Cristina Barbosa, Marina Ponce, Ricardo Andrade Furtado, Ana Carolina Pereira, Jairo Kenupp Bastos, Marcio Luis Andrade E Silva, Denise Crispim Tavares
    Abstract:

    Abstract The dibenzylbutyrolactone lignan (−)-hinokinin (HK) was obtained by partial synthesis from (−)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba . In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound's effect on the activity of the clastogens doxorubicin (DXR) and Methyl Methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128 μM. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity.

  • Effect of the dibenzylbutyrolactone lignan (−)-hinokinin on doxorubicin and Methyl Methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts
    Mutation Research Genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Flávia Aparecida Resende, Iara Maluf Tomazella, Lilian Cristina Barbosa, Marina Ponce, Ricardo Andrade Furtado, Ana Carolina Pereira, Marcio Luis Andrade E Silva, Jairo Kenupp Bastos, Denise Crispim Tavares
    Abstract:

    The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was obtained by partial synthesis from (-)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba. In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound`s effect on the activity of the clastogens doxorubicin (DXR) and Methyl Methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128 mu M. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity. (C) 2010 Elsevier B.V. All rights reserved.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Brazil)[2007/07211-6]FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paul

Flávia Aparecida Resende - One of the best experts on this subject based on the ideXlab platform.

  • Effect of the dibenzylbutyrolactone lignan (-)-hinokinin on doxorubicin and Methyl Methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts.
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Flávia Aparecida Resende, Iara Maluf Tomazella, Lilian Cristina Barbosa, Marina Ponce, Ricardo Andrade Furtado, Ana Carolina Pereira, Marcio Luis Andrade E Silva, Jairo Kenupp Bastos, Denise Crispim Tavares
    Abstract:

    Abstract The dibenzylbutyrolactone lignan (−)-hinokinin (HK) was obtained by partial synthesis from (−)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba . In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound's effect on the activity of the clastogens doxorubicin (DXR) and Methyl Methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128 μM. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity.

  • effect of the dibenzylbutyrolactone lignan hinokinin on doxorubicin and Methyl Methanesulfonate clastogenicity in v79 chinese hamster lung fibroblasts
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Flávia Aparecida Resende, Iara Maluf Tomazella, Lilian Cristina Barbosa, Marina Ponce, Ricardo Andrade Furtado, Ana Carolina Pereira, Jairo Kenupp Bastos, Marcio Luis Andrade E Silva, Denise Crispim Tavares
    Abstract:

    Abstract The dibenzylbutyrolactone lignan (−)-hinokinin (HK) was obtained by partial synthesis from (−)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba . In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound's effect on the activity of the clastogens doxorubicin (DXR) and Methyl Methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128 μM. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity.

  • Effect of the dibenzylbutyrolactone lignan (−)-hinokinin on doxorubicin and Methyl Methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts
    Mutation Research Genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Flávia Aparecida Resende, Iara Maluf Tomazella, Lilian Cristina Barbosa, Marina Ponce, Ricardo Andrade Furtado, Ana Carolina Pereira, Marcio Luis Andrade E Silva, Jairo Kenupp Bastos, Denise Crispim Tavares
    Abstract:

    The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was obtained by partial synthesis from (-)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba. In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound`s effect on the activity of the clastogens doxorubicin (DXR) and Methyl Methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128 mu M. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity. (C) 2010 Elsevier B.V. All rights reserved.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Brazil)[2007/07211-6]FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paul

H Shimada - One of the best experts on this subject based on the ideXlab platform.

  • Germ cell mutagenesis in lacZ transgenic mice treated with Methyl Methanesulfonate.
    Mutation research, 1997
    Co-Authors: S Itoh, M Miura, H Shimada
    Abstract:

    Mutagenesis induced by Methyl Methanesulfonate (MMS), a germ cell mutagen, in the testis and the sperm isolated from epididymis and vas deferens have been investigated using lacZ transgenic mice (Muta Mouse). Male Muta Mice were injected intraperitoneally with MMS at a dose of 80 mg/kg, a potent dominant lethal dose. Animals were killed on days 3 and 7 (Experiment 1) or days 10 and 14 (Experiment 2) after the treatment. Mutant frequencies (MFs) in the testis, sperm and spleen (Experiment 2 only) were analyzed by the positive selection system using E. coli C (GalE-) strain and phenyl beta-D-galactoside. The spontaneous MFs in the testis and sperm were 2.0-3.1 x 10(-5). No induction of mutation in the testis or sperm of the MMS-treated groups was observed at any sampling point. In the spleen, the spontaneous MF was approximately twice as high as that in the germ cells although the MF at each sampling point was almost the same as the spontaneous MF. MMS is known as a potent clastogen from the results of the dominant lethal assay and the micronucleus assay. The reason for the discrepancy between the results of these assays and the present results may have been insensitivity of the in vitro packaging to large deletion due to the failure to rescue the large deleted gene. It is suggested that the transgenic mouse assay using the in vitro packaging can not replace the dominant lethal assay in the case of MMS.

  • Germ cell mutagenesis in lacZ transgenic mice treated with Methyl Methanesulfonate.
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 1997
    Co-Authors: S Itoh, M Miura, H Shimada
    Abstract:

    Abstract Mutagenesis induced by Methyl Methanesulfonate (MMS), a germ cell mutagen, in the testis and the sperm isolated from epididymis and vas deferens have been investigated using lacZ transgenic mice (Muta™Mouse). Male Muta™Mice were injected intraperitoneally with MMS at a dose of 80 mg/kg, a potent dominant lethal dose. Animals were killed on days 3 and 7 (Experiment 1) or days 10 and 14 (Experiment 2) after the treatment. Mutant frequencies (MFs) in the testis, sperm and spleen (Experiment 2 only) were analyzed by the positive selection system using E. coli C ( GalE − ) strain and phenyl β- d -galactoside. The spontaneous MFs in the testis and sperm were 2.0–3.1×10 −5 . No induction of mutation in the testis or sperm of the MMS-treated groups was observed at any sampling point. In the spleen, the spontaneous MF was approximately twice as high as that in the germ cells although the MF at each sampling point was almost the same as the spontaneous MF. MMS is known as a potent clastogen from the results of the dominant lethal assay and the micronucleus assay. The reason for the discrepancy between the results of these assays and the present results may have been insensitivity of the in vitro packaging to large deletion due to the failure to rescue the large deleted gene. It is suggested that the transgenic mouse assay using the in vitro packaging can not replace the dominant lethal assay in the case of MMS.

Feng Zheng - One of the best experts on this subject based on the ideXlab platform.

  • determination of Methyl Methanesulfonate and ethyl Methanesulfonate in methanesulfonic acid by derivatization followed by high performance liquid chromatography with ultraviolet detection
    IEEE Journal of Solid-state Circuits, 2017
    Co-Authors: Jie Zhou, Xiangyuan Zheng, Wenyuan Liu, Feng Zheng
    Abstract:

    Methanesulfonic acid is routinely used in pharmaceuticals but can contain potentially genotoxic impurities such as Methyl Methanesulfonate and ethyl Methanesulfonate. The aim of this study was to develop a simple high-performance liquid chromatography with ultraviolet detection method for determining Methyl Methanesulfonate and ethyl Methanesulfonate in methanesulfonic acid. Samples (250 mg) in water/acetonitrile (200 μL) were first combined with 10.0 mol/L sodium hydroxide solution (270 μL). Then they were mixed with 2.0 mg/mL N,N-diethyldithiocarbamate (500 μL), diluted to 5 mL with N,N-diMethylacetamide and allowed to react at 80°C for 1 h. The derivatives were analyzed using gradient high-performance liquid chromatography with ultraviolet detection (277 nm) and structurally elucidated by liquid chromatography with mass spectrometry. With acetonitrile/5 mmol/L ammonium acetate solution as the eluent and 1 mL/min as the flow rate on a C18 column, the derivatives were eluted at 10.6 and 14.8 min. Good linearity (correlation coefficients > 0.999) and low limits of quantitation (0.6 ppm) were obtained. The recoveries were in the range of 80-115% with relative standard deviation < 5.0%. Finally, the established method was successfully used for the determination of Methyl Methanesulfonate and ethyl Methanesulfonate in methanesulfonic acid.

  • Determination of Methyl Methanesulfonate and ethyl Methanesulfonate in methanesulfonic acid by derivatization followed by high‐performance liquid chromatography with ultraviolet detection
    Journal of separation science, 2017
    Co-Authors: Jie Zhou, Xiangyuan Zheng, Wenyuan Liu, Feng Zheng
    Abstract:

    Methanesulfonic acid is routinely used in pharmaceuticals but can contain potentially genotoxic impurities such as Methyl Methanesulfonate and ethyl Methanesulfonate. The aim of this study was to develop a simple high-performance liquid chromatography with ultraviolet detection method for determining Methyl Methanesulfonate and ethyl Methanesulfonate in methanesulfonic acid. Samples (250 mg) in water/acetonitrile (200 μL) were first combined with 10.0 mol/L sodium hydroxide solution (270 μL). Then they were mixed with 2.0 mg/mL N,N-diethyldithiocarbamate (500 μL), diluted to 5 mL with N,N-diMethylacetamide and allowed to react at 80°C for 1 h. The derivatives were analyzed using gradient high-performance liquid chromatography with ultraviolet detection (277 nm) and structurally elucidated by liquid chromatography with mass spectrometry. With acetonitrile/5 mmol/L ammonium acetate solution as the eluent and 1 mL/min as the flow rate on a C18 column, the derivatives were eluted at 10.6 and 14.8 min. Good linearity (correlation coefficients > 0.999) and low limits of quantitation (0.6 ppm) were obtained. The recoveries were in the range of 80-115% with relative standard deviation < 5.0%. Finally, the established method was successfully used for the determination of Methyl Methanesulfonate and ethyl Methanesulfonate in methanesulfonic acid.

Marina Ponce - One of the best experts on this subject based on the ideXlab platform.

  • Effect of the dibenzylbutyrolactone lignan (-)-hinokinin on doxorubicin and Methyl Methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts.
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Flávia Aparecida Resende, Iara Maluf Tomazella, Lilian Cristina Barbosa, Marina Ponce, Ricardo Andrade Furtado, Ana Carolina Pereira, Marcio Luis Andrade E Silva, Jairo Kenupp Bastos, Denise Crispim Tavares
    Abstract:

    Abstract The dibenzylbutyrolactone lignan (−)-hinokinin (HK) was obtained by partial synthesis from (−)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba . In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound's effect on the activity of the clastogens doxorubicin (DXR) and Methyl Methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128 μM. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity.

  • effect of the dibenzylbutyrolactone lignan hinokinin on doxorubicin and Methyl Methanesulfonate clastogenicity in v79 chinese hamster lung fibroblasts
    Mutation Research-genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Flávia Aparecida Resende, Iara Maluf Tomazella, Lilian Cristina Barbosa, Marina Ponce, Ricardo Andrade Furtado, Ana Carolina Pereira, Jairo Kenupp Bastos, Marcio Luis Andrade E Silva, Denise Crispim Tavares
    Abstract:

    Abstract The dibenzylbutyrolactone lignan (−)-hinokinin (HK) was obtained by partial synthesis from (−)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba . In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound's effect on the activity of the clastogens doxorubicin (DXR) and Methyl Methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128 μM. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity.

  • Effect of the dibenzylbutyrolactone lignan (−)-hinokinin on doxorubicin and Methyl Methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts
    Mutation Research Genetic Toxicology and Environmental Mutagenesis, 2010
    Co-Authors: Flávia Aparecida Resende, Iara Maluf Tomazella, Lilian Cristina Barbosa, Marina Ponce, Ricardo Andrade Furtado, Ana Carolina Pereira, Marcio Luis Andrade E Silva, Jairo Kenupp Bastos, Denise Crispim Tavares
    Abstract:

    The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was obtained by partial synthesis from (-)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba. In view of the trypanocidal activity of HK and its potential as a lead compound for drug development, evaluation of its possible genotoxic activity is required. We have tested HK for possible genotoxicity and evaluated the compound`s effect on the activity of the clastogens doxorubicin (DXR) and Methyl Methanesulfonate (MMS) in the micronucleus (MN) assay with Chinese hamster lung fibroblast V79 cells. HK alone did not induce MN, at concentrations up to 128 mu M. In combined treatments, HK reduced the frequency of MN induced by MMS. With respect to DXR, HK exerted a protective effect at lower concentrations, but at higher concentrations it potentiated DXR clastogenicity. (C) 2010 Elsevier B.V. All rights reserved.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Brazil)[2007/07211-6]FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paul