The Experts below are selected from a list of 30 Experts worldwide ranked by ideXlab platform

Bill Gillette - One of the best experts on this subject based on the ideXlab platform.

Kanakaris, Georgios P. - One of the best experts on this subject based on the ideXlab platform.

  • An automated platform for magnetic-based Microbeads manipulation
    2011
    Co-Authors: Κανακάρης, Γεώργιος Π., Kanakaris, Georgios P.
    Abstract:

    265 σ.Στη παρούσα εργασία επιχειρείται η μελέτη, ο σχεδιασμός και η κατασκευή μιάς πλατφόρμας που θα πραγματοποιεί, χωρίς ανθώπινη παρέμβαση, βιοχημικά πειράματα ELISA για την χαρτογράφηση του ανθρώπινου κυττάρου. Στα επόμενα κεφάλαια θα αναλυθεί η χρησιμότητα αυτών των πειραμάτων, η διαδικασία διεξαγωγής τους και οι τεχνολογίες που χρησιμοποιούνται σε επίπεδο υλικών και εξοπλισμού σε εργαστήρια βιοτεχνολογίας και νοσοκομεία.Στη συνέχεια θα τεθούν προδιαγραφές για τα χαρακτηριστικά της πλατφόρμας και θα παρουσιαστεί ο προκαταρτικός σχεδιασμός της συσκευής. Θα παρουσιαστούν κάποια πειράματα και μία πειραματική διάταξη που χρησιμοποιήθηκε για τον προσδιορισμό των μεταβλητών του προβλήματος. Θα παρουσιαστεί ο γενικός σχεδιασμός της πλατφόρμας και έπειτα θα εστιάσουμε σε κάθε υποσύστημα χωριστά. Στο τέλος κάθε υποσυστήματος μπορεί κανείς να δει τα κατασκευαστικά σχέδια για τα μέρη που το απαρτίζουν.Introduction: The ever increasing use of high-throughput methods in biology has created the need of automated platforms for full sample preparation with minimal user intervention. While various products have been developed to assist researchers in those tasks, they are mostly based on liquid handling modules that move on a planar surface and mimic tasks perform by humans in a lab bench. In this work, we propose an automated platform where various assay steps are performed on a vertical rather a horizontal format. The apparatus in based on manipulation of magnetic beads where bead trapping and washing is performed inside pipette tips, thus maximizing bead-transfer efficiency and simplifying assay execution. Using those principles we are currently designing a prototype of a fully automated platform specifically made for the preparation of ELISA Microbead assays. Methods: Magnetic Microbead Technology: Using miniature retractable Neodymium PM’s and magnetic shielding we accomplish Microbead trapping. Pipetting / Washing : We introduce an embedded liquid handling/washing system whose operating principle allows it to handle sample volumes from 10ul and above and can perform washing steps within the pipette tip. Precision of motion: Using a z-only degree of freedom for the liquid handler and assorted hard lock positioning systems we ensure an accurate positioning system. Temperature control: Using thermoelectric coolers/heaters we developed temperature controlled stations inside the platform to secure the samples. Results/Discussion: The vertical orientation of plates guarantees a very small footprint that take up as much space as a large computer case. The new magnetic bead handling technique -if successful- allows for a low cost, easy to use, flexible and reliable platform for proteomic assays.Γεώργιος Π. Κανακάρη

Κανακάρης, Γεώργιος Π. - One of the best experts on this subject based on the ideXlab platform.

  • An automated platform for magnetic-based Microbeads manipulation
    2011
    Co-Authors: Κανακάρης, Γεώργιος Π., Kanakaris, Georgios P.
    Abstract:

    265 σ.Στη παρούσα εργασία επιχειρείται η μελέτη, ο σχεδιασμός και η κατασκευή μιάς πλατφόρμας που θα πραγματοποιεί, χωρίς ανθώπινη παρέμβαση, βιοχημικά πειράματα ELISA για την χαρτογράφηση του ανθρώπινου κυττάρου. Στα επόμενα κεφάλαια θα αναλυθεί η χρησιμότητα αυτών των πειραμάτων, η διαδικασία διεξαγωγής τους και οι τεχνολογίες που χρησιμοποιούνται σε επίπεδο υλικών και εξοπλισμού σε εργαστήρια βιοτεχνολογίας και νοσοκομεία.Στη συνέχεια θα τεθούν προδιαγραφές για τα χαρακτηριστικά της πλατφόρμας και θα παρουσιαστεί ο προκαταρτικός σχεδιασμός της συσκευής. Θα παρουσιαστούν κάποια πειράματα και μία πειραματική διάταξη που χρησιμοποιήθηκε για τον προσδιορισμό των μεταβλητών του προβλήματος. Θα παρουσιαστεί ο γενικός σχεδιασμός της πλατφόρμας και έπειτα θα εστιάσουμε σε κάθε υποσύστημα χωριστά. Στο τέλος κάθε υποσυστήματος μπορεί κανείς να δει τα κατασκευαστικά σχέδια για τα μέρη που το απαρτίζουν.Introduction: The ever increasing use of high-throughput methods in biology has created the need of automated platforms for full sample preparation with minimal user intervention. While various products have been developed to assist researchers in those tasks, they are mostly based on liquid handling modules that move on a planar surface and mimic tasks perform by humans in a lab bench. In this work, we propose an automated platform where various assay steps are performed on a vertical rather a horizontal format. The apparatus in based on manipulation of magnetic beads where bead trapping and washing is performed inside pipette tips, thus maximizing bead-transfer efficiency and simplifying assay execution. Using those principles we are currently designing a prototype of a fully automated platform specifically made for the preparation of ELISA Microbead assays. Methods: Magnetic Microbead Technology: Using miniature retractable Neodymium PM’s and magnetic shielding we accomplish Microbead trapping. Pipetting / Washing : We introduce an embedded liquid handling/washing system whose operating principle allows it to handle sample volumes from 10ul and above and can perform washing steps within the pipette tip. Precision of motion: Using a z-only degree of freedom for the liquid handler and assorted hard lock positioning systems we ensure an accurate positioning system. Temperature control: Using thermoelectric coolers/heaters we developed temperature controlled stations inside the platform to secure the samples. Results/Discussion: The vertical orientation of plates guarantees a very small footprint that take up as much space as a large computer case. The new magnetic bead handling technique -if successful- allows for a low cost, easy to use, flexible and reliable platform for proteomic assays.Γεώργιος Π. Κανακάρη

K. V. Chalam - One of the best experts on this subject based on the ideXlab platform.

  • environment beyond neutralizing VEGF
    2013
    Co-Authors: Rajesh K. Sharma, Anna T. Rogojina, K. V. Chalam
    Abstract:

    Purpose: Vascular endothelial growth factor (VEGF) plays a key role in neovascularization by stimulating the proliferation and migration of vascular endothelial cells. The anti-VEGF therapy bevacizumab acts by binding to VEGF and preventing its effects. However, this linear interaction represents only a partial view of the pathobiology of neovascular diseases and the anti-VEGF treatment. To obtain an integrated view of the processes involved in VEGF-related ocular pathologies, we applied a systems approach and investigated whether intravitreal bevacizumab injections have a global effect in normalizing the ocular physiology perturbed by the disease. Methods: We analyzed 90 analytes representing various pathophysiological processes in aqueous humor. The samples were obtained from eight patients receiving intravitreal bevacizumab injections for various ocular VEGF-related conditions. The samples were obtained before and after the injection and were analyzed using Microbead Technology developed by Luminex xMAP. Results: Forty-three analytes were detected above the sensitivity of the assay both in pre- and post-injection samples. Of these, normal values of 41 analytes were known and these analytes were further analyzed. The detected analytes included relevant markers such as VEGF, C reactive protein, glutathione, and cytokines. We identified 24 markers that were perturbed more than 1.5 fold in diseased samples (pre-injection) compared to normal levels. The levels of perturbed analytes were compared in post-treatment samples. The results demonstrated an unequivocal trend toward normalizatio

Mackinnon, Laurel T. - One of the best experts on this subject based on the ideXlab platform.

  • Exercise and T-lymphocyte function: a comparison of proliferation in PBMC and NK cell-depleted PBMC culture
    'American Physiological Society', 2002
    Co-Authors: Green, Katherine J., Rowbottom, David G., Mackinnon, Laurel T.
    Abstract:

    This study utilized recently developed Microbead Technology to remove natural killer (NK) cells from peripheral blood mononuclear cell (PBMC) preparations to determine the effect of acute exercise on T-lymphocyte function, independent of changes in lymphocyte subpopulations. Twelve well-trained male runners completed a 60-min exercise trial at 95% ventilatory threshold and a no-exercise control trial. Six blood samples were taken at each session: before exercise, midexercise, immediately after exercise, and 30, 60, and 90 min after exercise. Isolated PBMC and NK cell-depleted PBMC were stimulated with the mitogen phytohemagglutinin. Cellular proliferation was assessed by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye uptake. In the PBMC cultures, there was a significantly lower mitogen response to phytohemagglutinin in exercise compared with the control condition immediately postexercise. There were no significant differences between the control and exercise conditions in NK cell-depleted PBMC cultures or in the responses adjusted for the percentage of CD3 cells. The present findings do not support the view that T-lymphocyte function is reduced after exercise