The Experts below are selected from a list of 17181 Experts worldwide ranked by ideXlab platform
Chamindie Punyadeera - One of the best experts on this subject based on the ideXlab platform.
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The Isolation and Characterization of Circulating Tumor Cells from Head and Neck Cancer Patient Blood Samples Using Spiral Microfluidic Technology.
Methods in molecular biology (Clifton N.J.), 2019Co-Authors: Arutha Kulasinghe, Majid Ebrahimi Warkiani, Chamindie PunyadeeraAbstract:Metastasis is responsible for 90% of cancer-related deaths. The study of circulating tumor cells (CTCs) enables the study of the units of disease responsible for the process of metastasis. While the biology of the primary tissue is relatively known, little is understood about the cells en route to distant sites. Here we describe the isolation of CTCs using the spiral Microfluidic Technology for the efficient sorting of CTCs from head and neck cancer (HNC) patient blood samples. Furthermore, the molecular characterization of CTCs can aid in stratifying patients for targeted therapy such as immunotherapy, which is having a profound impact in the treatment of metastatic HNC.
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The Use of Microfluidic Technology for Cancer Applications and Liquid Biopsy.
Micromachines, 2018Co-Authors: Arutha Kulasinghe, Chamindie Punyadeera, Majid Ebrahimi WarkianiAbstract:There is growing awareness for the need of early diagnostic tools to aid in point-of-care testing in cancer. Tumor biopsy remains the conventional means in which to sample a tumor and often presents with challenges and associated risks. Therefore, alternative sources of tumor biomarkers is needed. Liquid biopsy has gained attention due to its non-invasive sampling of tumor tissue and ability to serially assess disease via a simple blood draw over the course of treatment. Among the leading technologies developing liquid biopsy solutions, Microfluidics has recently come to the fore. Microfluidic platforms offer cellular separation and analysis platforms that allow for high throughout, high sensitivity and specificity, low sample volumes and reagent costs and precise liquid controlling capabilities. These characteristics make Microfluidic Technology a promising tool in separating and analyzing circulating tumor biomarkers for diagnosis, prognosis and monitoring. In this review, the characteristics of three kinds of circulating tumor markers will be described in the context of cancer, circulating tumor cells (CTCs), exosomes, and circulating tumor DNA (ctDNA). The review will focus on how the introduction of Microfluidic technologies has improved the separation and analysis of these circulating tumor markers.
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Enrichment of circulating head and neck tumour cells using spiral Microfluidic Technology.
Scientific reports, 2017Co-Authors: Arutha Kulasinghe, Majid Ebrahimi Warkiani, Thao Huynh Phuoc Tran, Tony Blick, Kenneth J. O'byrne, Erik W. Thompson, Colleen C. Nelson, Liz Kenny, Chamindie PunyadeeraAbstract:Whilst locoregional control of head and neck cancers (HNCs) has improved over the last four decades, long-term survival has remained largely unchanged. A possible reason for this is that the rate of distant metastasis has not changed. Such disseminated disease is reflected in measurable levels of cancer cells in the blood of HNC patients, referred to as circulating tumour cells (CTCs). Numerous marker-independent techniques have been developed for CTC isolation and detection. Recently, Microfluidics-based platforms have come to the fore to avoid molecular bias. In this pilot, proof of concept study, we evaluated the use of the spiral Microfluidic chip for CTC enrichment and subsequent detection in HNC patients. CTCs were detected in 13/24 (54%) HNC patients, representing both early to late stages of disease. Importantly, in 7/13 CTC-positive patients, CTC clusters were observed. This is the first study to use spiral Microfluidics Technology for CTC enrichment in HNC.
Matthew B. Wheeler - One of the best experts on this subject based on the ideXlab platform.
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reduction of polyspermic penetration using biomimetic Microfluidic Technology during in vitro fertilization
Lab on a Chip, 2005Co-Authors: Sherrie G Clark, Matthew B. Wheeler, David J Beebe, Kathyrn Haubert, Edward C FergusonAbstract:Efforts to improve the in vitro embryo production process in pigs have included modifying culture medium and number of spermatozoa inseminated in order to reduce the incidence of polyspermy. Polyspermy is a pathological condition which results in aberrant embryonic development. The microchannels are designed to more closely mimic the function of the oviduct and create a flow pattern of spermatozoa past the oocytes similar to the pattern in the oviduct. In vitro fertilization of porcine oocytes in the microchannels has produced a higher incidence of monospermic penetration (p < 0.05) as compared to the oocytes fertilized in the traditional microdrop system with comparable penetration and male pronucleus formation rates. Additionally, cleavage rates of the embryos as well as development to the blastocyst stage are similar. Here we demonstrate that the biomimetic microchannel in vitro fertilization system can reduce polyspermy and, therefore, increase the number of potentially viable embryos without reducing the overall in vitro production efficiency.
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Microfluidic Technology for assisted reproduction
Theriogenology, 2002Co-Authors: David J Beebe, E M Walters, Matthew B. Wheeler, H C Zeringue, S RatyAbstract:Abstract The physical tools used in assisted reproduction have changed little over several decades. Microfluidics is an emerging Technology that allows a fresh examination of the way assisted reproduction is performed. Here we review our work to develop Microfluidic devices to perform the functions required in assisted reproduction. These functions include loading/unloading, culture, chemical manipulation, and mechanical manipulation of embryos and oocytes. Basic Microfluidic theory and Microfluidic device design and operation are discussed. Results are presented for mechanical removal of cumulus cells and for embryo culture. Results suggest that Microfluidic systems will lead to improved efficiencies in assisted reproduction.
Arutha Kulasinghe - One of the best experts on this subject based on the ideXlab platform.
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Application of Microfluidic Technology in cancer research and therapy.
Advances in clinical chemistry, 2020Co-Authors: Shohreh Azadi, Arutha Kulasinghe, Pritam Bordhan, Majid Ebrahimi WarkianiAbstract:Cancer is a heterogeneous disease that requires a multimodal approach to diagnose, manage and treat. A better understanding of the disease biology can lead to identification of novel diagnostic/prognostic biomarkers and the discovery of the novel therapeutics with the goal of improving patient outcomes. Employing advanced technologies can facilitate this, enabling better diagnostic and treatment for cancer patients. In this regard, Microfluidic Technology has emerged as a promising tool in the studies of cancer, including single cancer cell analysis, modeling angiogenesis and metastasis, drug screening and liquid biopsy. Microfluidic technologies have opened new ways to study tumors in the preclinical and clinical settings. In this chapter, we highlight novel application of this Technology in area of fundamental, translational and clinical cancer research.
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The Isolation and Characterization of Circulating Tumor Cells from Head and Neck Cancer Patient Blood Samples Using Spiral Microfluidic Technology.
Methods in molecular biology (Clifton N.J.), 2019Co-Authors: Arutha Kulasinghe, Majid Ebrahimi Warkiani, Chamindie PunyadeeraAbstract:Metastasis is responsible for 90% of cancer-related deaths. The study of circulating tumor cells (CTCs) enables the study of the units of disease responsible for the process of metastasis. While the biology of the primary tissue is relatively known, little is understood about the cells en route to distant sites. Here we describe the isolation of CTCs using the spiral Microfluidic Technology for the efficient sorting of CTCs from head and neck cancer (HNC) patient blood samples. Furthermore, the molecular characterization of CTCs can aid in stratifying patients for targeted therapy such as immunotherapy, which is having a profound impact in the treatment of metastatic HNC.
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The Use of Microfluidic Technology for Cancer Applications and Liquid Biopsy.
Micromachines, 2018Co-Authors: Arutha Kulasinghe, Chamindie Punyadeera, Majid Ebrahimi WarkianiAbstract:There is growing awareness for the need of early diagnostic tools to aid in point-of-care testing in cancer. Tumor biopsy remains the conventional means in which to sample a tumor and often presents with challenges and associated risks. Therefore, alternative sources of tumor biomarkers is needed. Liquid biopsy has gained attention due to its non-invasive sampling of tumor tissue and ability to serially assess disease via a simple blood draw over the course of treatment. Among the leading technologies developing liquid biopsy solutions, Microfluidics has recently come to the fore. Microfluidic platforms offer cellular separation and analysis platforms that allow for high throughout, high sensitivity and specificity, low sample volumes and reagent costs and precise liquid controlling capabilities. These characteristics make Microfluidic Technology a promising tool in separating and analyzing circulating tumor biomarkers for diagnosis, prognosis and monitoring. In this review, the characteristics of three kinds of circulating tumor markers will be described in the context of cancer, circulating tumor cells (CTCs), exosomes, and circulating tumor DNA (ctDNA). The review will focus on how the introduction of Microfluidic technologies has improved the separation and analysis of these circulating tumor markers.
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Enrichment of circulating head and neck tumour cells using spiral Microfluidic Technology.
Scientific reports, 2017Co-Authors: Arutha Kulasinghe, Majid Ebrahimi Warkiani, Thao Huynh Phuoc Tran, Tony Blick, Kenneth J. O'byrne, Erik W. Thompson, Colleen C. Nelson, Liz Kenny, Chamindie PunyadeeraAbstract:Whilst locoregional control of head and neck cancers (HNCs) has improved over the last four decades, long-term survival has remained largely unchanged. A possible reason for this is that the rate of distant metastasis has not changed. Such disseminated disease is reflected in measurable levels of cancer cells in the blood of HNC patients, referred to as circulating tumour cells (CTCs). Numerous marker-independent techniques have been developed for CTC isolation and detection. Recently, Microfluidics-based platforms have come to the fore to avoid molecular bias. In this pilot, proof of concept study, we evaluated the use of the spiral Microfluidic chip for CTC enrichment and subsequent detection in HNC patients. CTCs were detected in 13/24 (54%) HNC patients, representing both early to late stages of disease. Importantly, in 7/13 CTC-positive patients, CTC clusters were observed. This is the first study to use spiral Microfluidics Technology for CTC enrichment in HNC.
Majid Ebrahimi Warkiani - One of the best experts on this subject based on the ideXlab platform.
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Application of Microfluidic Technology in cancer research and therapy.
Advances in clinical chemistry, 2020Co-Authors: Shohreh Azadi, Arutha Kulasinghe, Pritam Bordhan, Majid Ebrahimi WarkianiAbstract:Cancer is a heterogeneous disease that requires a multimodal approach to diagnose, manage and treat. A better understanding of the disease biology can lead to identification of novel diagnostic/prognostic biomarkers and the discovery of the novel therapeutics with the goal of improving patient outcomes. Employing advanced technologies can facilitate this, enabling better diagnostic and treatment for cancer patients. In this regard, Microfluidic Technology has emerged as a promising tool in the studies of cancer, including single cancer cell analysis, modeling angiogenesis and metastasis, drug screening and liquid biopsy. Microfluidic technologies have opened new ways to study tumors in the preclinical and clinical settings. In this chapter, we highlight novel application of this Technology in area of fundamental, translational and clinical cancer research.
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The Isolation and Characterization of Circulating Tumor Cells from Head and Neck Cancer Patient Blood Samples Using Spiral Microfluidic Technology.
Methods in molecular biology (Clifton N.J.), 2019Co-Authors: Arutha Kulasinghe, Majid Ebrahimi Warkiani, Chamindie PunyadeeraAbstract:Metastasis is responsible for 90% of cancer-related deaths. The study of circulating tumor cells (CTCs) enables the study of the units of disease responsible for the process of metastasis. While the biology of the primary tissue is relatively known, little is understood about the cells en route to distant sites. Here we describe the isolation of CTCs using the spiral Microfluidic Technology for the efficient sorting of CTCs from head and neck cancer (HNC) patient blood samples. Furthermore, the molecular characterization of CTCs can aid in stratifying patients for targeted therapy such as immunotherapy, which is having a profound impact in the treatment of metastatic HNC.
-
The Use of Microfluidic Technology for Cancer Applications and Liquid Biopsy.
Micromachines, 2018Co-Authors: Arutha Kulasinghe, Chamindie Punyadeera, Majid Ebrahimi WarkianiAbstract:There is growing awareness for the need of early diagnostic tools to aid in point-of-care testing in cancer. Tumor biopsy remains the conventional means in which to sample a tumor and often presents with challenges and associated risks. Therefore, alternative sources of tumor biomarkers is needed. Liquid biopsy has gained attention due to its non-invasive sampling of tumor tissue and ability to serially assess disease via a simple blood draw over the course of treatment. Among the leading technologies developing liquid biopsy solutions, Microfluidics has recently come to the fore. Microfluidic platforms offer cellular separation and analysis platforms that allow for high throughout, high sensitivity and specificity, low sample volumes and reagent costs and precise liquid controlling capabilities. These characteristics make Microfluidic Technology a promising tool in separating and analyzing circulating tumor biomarkers for diagnosis, prognosis and monitoring. In this review, the characteristics of three kinds of circulating tumor markers will be described in the context of cancer, circulating tumor cells (CTCs), exosomes, and circulating tumor DNA (ctDNA). The review will focus on how the introduction of Microfluidic technologies has improved the separation and analysis of these circulating tumor markers.
-
Enrichment of circulating head and neck tumour cells using spiral Microfluidic Technology.
Scientific reports, 2017Co-Authors: Arutha Kulasinghe, Majid Ebrahimi Warkiani, Thao Huynh Phuoc Tran, Tony Blick, Kenneth J. O'byrne, Erik W. Thompson, Colleen C. Nelson, Liz Kenny, Chamindie PunyadeeraAbstract:Whilst locoregional control of head and neck cancers (HNCs) has improved over the last four decades, long-term survival has remained largely unchanged. A possible reason for this is that the rate of distant metastasis has not changed. Such disseminated disease is reflected in measurable levels of cancer cells in the blood of HNC patients, referred to as circulating tumour cells (CTCs). Numerous marker-independent techniques have been developed for CTC isolation and detection. Recently, Microfluidics-based platforms have come to the fore to avoid molecular bias. In this pilot, proof of concept study, we evaluated the use of the spiral Microfluidic chip for CTC enrichment and subsequent detection in HNC patients. CTCs were detected in 13/24 (54%) HNC patients, representing both early to late stages of disease. Importantly, in 7/13 CTC-positive patients, CTC clusters were observed. This is the first study to use spiral Microfluidics Technology for CTC enrichment in HNC.
Su Chen - One of the best experts on this subject based on the ideXlab platform.
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Synthesis of quantum dots based on Microfluidic Technology
Current Opinion in Chemical Engineering, 2020Co-Authors: Li Guoxing, Rui Cheng, Su ChenAbstract:In recent years, quantum dots (QDs) have attracted considerable research interests in the fields of biomedicine, energy, optoelectronics, and photocatalysis owing to their unmatched luminescent properties, excellent fluorescence stability, and relatively low cost. Microfluidic Technology for the synthesis of QDs has shown unparalleled superiority as a result of its simple operation, fast heat and mass transfer, and precise control of reaction parameters. In this review, we will focus on the application of Microfluidic platforms in the synthesis of single core and core-shell semiconductor QDs, perovskite QDs (PQDs) and carbon quantum dots (CQDs). We do expect that this review will provide new insights for the fabrication of various QDs and nanomaterials by Microfluidic reaction platforms.