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Takuji Kudo - One of the best experts on this subject based on the ideXlab platform.
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Micromonospora radicis sp. nov., isolated from roots of Azadirachta indica var. siamensis Valenton, and reclassification of Jishengella zingiberis as Micromonospora zingiberis comb. nov.
International journal of systematic and evolutionary microbiology, 2019Co-Authors: Nattakorn Kuncharoen, Takuji Kudo, Pattama Pittayakhajonwut, Masahiro Yuki, Moriya Okuma, Somboon TanasupawatAbstract:A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach. Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively. Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora . The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content. The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T. Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora : cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids. Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora , for which the name Micromonospora radicis sp. nov. is proposed. The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T). This study also proposed that J. zingiberis is transferred to the genus Micromonospora as Micromonospora zingiberis comb. nov. (type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T).
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Micromonospora caldifontis sp. nov., isolated from hot spring soil.
International journal of systematic and evolutionary microbiology, 2019Co-Authors: Chitti Thawai, Somboon Tanasupawat, Takuji KudoAbstract:A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil. The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora . The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphoglycolipid. The major menaquinones were MK-10(H6) and MK-10(H4). The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 70.5 mol%. 16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %). The physiological and DNA–DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species. Thus, the strain represents a novel species of the genus Micromonospora , for which the name Micromonospora caldifontis sp. nov. is proposed. The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
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Micromonospora azadirachtae sp. nov., isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton
Antonie van Leeuwenhoek, 2019Co-Authors: Nattakorn Kuncharoen, Moriya Ohkuma, Takuji Kudo, Somboon TanasupawatAbstract:A Gram-stain positive actinomycete, strain AZ1-19^T, isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton, collected from Chachoengsao province, Thailand, was characterised taxonomically by using a polyphasic approach. Strain AZ1-19^T was found to have characteristics consistent with those of members of the genus Micromonospora . The cell wall peptidoglycan of the strain was found to contain meso -diaminopimelic acid. The predominant phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The characteristic whole-cell sugars were identified as glucose, xylose, galactose and mannose. The major menaquinones were found to be MK-9(H_6), MK-10(H_6) and MK-10(H_8), and the major cellular fatty acids were identified as iso -C_16:0, iso -C_15:0, anteiso -C_15:0 and anteiso -C_17:0. Comparative analysis of 16S rRNA gene sequences revealed that strain AZ1-19^T is closely related to Micromonospora costi CS1-12^T (98.75% similarity), Micromonospora avicenniae 268506^T (98.75%), Micromonospora haikouensis 232617^T (98.68%) and Micromonospora siamensis TT2-4^T (98.61%), whilst the corresponding phylogenetic analysis based on partial gyrase subunit B ( gyr B) gene sequences indicated that strain AZ1-19^T forms a clade with M. avicenniae 268506^T with a high bootstrap value. The DNA G + C content was determined to be 69.8 mol%. Moreover, a combination of DNA–DNA relatedness values and some phenotypic and chemotaxonomic properties indicated that the strain could be distinguished from closely related species. Therefore, it is considered that strain AZ1-19^T represents a novel Micromonospora species for which the name Micromonospora azadirachtae sp. nov. is proposed. The type strain is AZ1-19^T (= KCTC 39941^T = NBRC 112784^T = JCM 32148^T = TISTR 2559^T).
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Micromonospora sediminis sp. nov., isolated from mangrove sediment.
International journal of systematic and evolutionary microbiology, 2016Co-Authors: Wongsakorn Phongsopitanun, Khanit Suwanborirux, Takuji Kudo, Pattama Pittayakhajonwut, Moriya Ohkuma, Somboon TanasupawatAbstract:The novel actinomycete, strain CH3-3T, was isolated from mangrove sediment collected from Chonburi Province, Thailand. On the basis of its spore morphology and chemotaxonomic characteristics, the strain belonged to the genus Micromonospora . It contained meso-diaminopimelic acid, glucose, mannose, xylose, ribose and rhamnose in the whole-cell hydrolysate, MK-10(H4), MK-10(H6) and MK-10(H8) as major menaquinones, and iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 as major cellular fatty acids. blast analysis of 16S rRNA gene sequences revealed that the strain showed highest similarity with Micromonospora palomenae NEAU-CX1T (98.97 %) and Micromonospora coxensis 2–30-b(28)T (98.97 %). Based on phylogenetic tree analysis of the 16S rRNA gene sequence, the strain formed a cluster with M. palomenae NEAU-CX1T, Micromonospora halophytica DSM 43171T, M. coxensis 2-30-b(28)T and Micromonospora purpureochromogenes DSM 43821T. On the basis of phenotypic differences and DNA–DNA relatedness evidence, strain CH3-3T could be clearly distinguished from the closely related species of the genus Micromonospora and represents a novel species of the genus Micromonospora for which the name Micromonospora sediminis sp. nov. is proposed. The type strain is CH3-3T (=JCM 18523T=PCU 350T=TISTR 2396T).
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Micromonospora fluostatini sp. nov., isolated from marine sediment.
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Wongsakorn Phongsopitanun, Khanit Suwanborirux, Takuji Kudo, Mihoko Mori, Kazuro Shiomi, Pattama Pittayakhajonwut, Somboon TanasupawatAbstract:The novel actinomycete strain PWB-003T, which produced fluostatins B and C antibiotics, was isolated from nearshore sediment collected from Panwa Cape, Phuket Province, Thailand. Data from the present polyphasic study indicated that strain PWB-003T represented a member of the genus Micromonospora. It produced single spores on substrate mycelia and contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. The predominant menaquinone was MK-10 (H4). Cellular fatty acids comprised C18 : 1ω9c, iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0. On the basis of 16S rRNA gene sequence similarity analysis, the novel strain was closely related to Micromonospora eburnea LK2-10T (99.38 %), Micromonospora chaiyaphumensis MC5-1T (99.16 %), Micromonospora yangpuensis FXJ6.011T (98.97 %), Micromonospora echinaurantiaca DSM 43904T (98.97 %), Micromonospora pallida DSM 43817T (98.97 %), Micromonospora sagamiensis DSM 43912T and Micromonospora auratinigra JCM 12357T (both 98.97 %). The G+C content of the DNA was 74.5 mol%. DNA–DNA relatedness values among strain PWB-003T and related type strains ranged from 11.3 ± 1.3 to 38.8 ± 1.1 %. On the basis of these observations, strain PWB-003T could be distinguished from its closely related type strains and is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora fluostatini sp. nov. (type strain PWB-003T = JCM 30529T = PCU 341T = TISTR 2345T) is proposed.
Martha E Trujillo - One of the best experts on this subject based on the ideXlab platform.
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High taxonomic diversity of Micromonospora strains isolated from Medicago sativa nodules in Western Spain and Australia.
Systematic and applied microbiology, 2019Co-Authors: Pilar Martínez-hidalgo, Martha E Trujillo, José David Flores-félix, Encarna Velázquez, Lambert Bräu, Eustoquio Martínez-molinaAbstract:The genus Micromonospora has been found in nodules of several legumes and some new species of this genus were isolated from these plant organs. In this study we analysed the taxonomic diversity of Micromonospora strains isolated from alfalfa nodules in Spain and Australia on the basis of three phylogenetic markers, the rrs and gyrB genes and 16S-23S intergenic spacer (ITS). The genome analysis of selected strains representative of different clusters or lineages found after rrs, gyrB and ITS analyses confirmed the results obtained with these phylogenetic markers. They showed that the analysed strains belong to at least 18 Micromonospora species including previously described ones, such as Micromonospora noduli, Micromonospora ureilytica, Micromonospora taraxaci, Micromonospora zamorensis, Micromonospora aurantiaca and Micromonospora tulbaghiae. Most of these strains belong to undescribed species of Micromonospora showing the high taxonomic diversity of strains from this genus inhabiting alfalfa nodules. Although Micromonospora strains are not able to induce the formation of these nodules, and it seems that they do not contribute to fix atmospheric nitrogen, they could play a role related with the mechanisms of plant growth promotion and pathogen protection presented by Micromonospora strains isolated from legume nodules.
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a study of three bacteria isolated from marine sediment and description of Micromonospora globispora sp nov
Systematic and Applied Microbiology, 2019Co-Authors: Lorena Carro, Hans-peter Klenk, Martha E Trujillo, Jose M Igual, Demet Cetin, Ayset Veyisoglu, Nevzat SahinAbstract:Abstract During a study looking for the isolation of new actinobacteria strains with potential for antibiotic production from deep marine sediment, three strains were collected with a morphology similar to the one described for the Micromonospora genus. A polyphasic study was designed to determine the taxonomic affiliation of the strains S2901T, S2903, and S2904. All the strains showed chemotaxonomic properties in line with their classification in the genus Micromonospora, meso-diaminopimelic acid in the wall peptidoglycan, a tetrahydrogenated menaquinone with nine isoprene units as major respiratory quinone, iso-C15:0 and iso-C16:0 as major fatty acids and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids. The 16S rRNA gene sequences of strain S2901T, S2903, and S2904 showed the highest similarity (99.2%) with the type strain of Micromonospora halophytica DSM 43171T, forming an independent branch in the phylogenetic gene tree. Their independent position was confirmed with gyrB gene and MLSA phylogenies. Whole genome sequences confirmed by digital DNA-DNA hybridization analysis that the isolates should be assigned to a new species within the genus Micromonospora for which the name Micromonospora globispora sp. nov. (S2901T, S2903 and S2904) is proposed.
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Micromonospora phytophila sp. nov. and Micromonospora luteiviridis sp. nov., isolated as natural inhabitants of plant nodules
International journal of systematic and evolutionary microbiology, 2017Co-Authors: Lorena Carro, Hans-peter Klenk, Aysel Veyisoglu, Cathrin Spröer, Nevzat Sahin, Raúl Riesco, Martha E TrujilloAbstract:Two actinobacterial isolates, strains SG15T and SGB14T, were recovered through a microbial diversity study of nitrogen fixing nodules from Pisum sativum plants collected in Salamanca (Spain). The taxonomic status of these isolates was determined using a polyphasic approach and both presented chemotaxonomic and morphological properties consistent with their classification in the genus Micromonospora . For strains SG15T and SGB14T, the highest 16S rRNA gene sequence similarities were observed with Micromonospora coxensis JCM 13248T (99.2 %) and Micromonospora purpureochromogenes DSM 43821T (99.4 %), respectively. However, strains SG15T and SGB14T were readily distinguished from their phylogenetic neighbours both genetically and phenotypically indicating that they represent two new Micromonospora species. The following names are proposed for these species: Micromonospora phytophila sp. nov. type strain SG15T (=CECT 9369T; =DSM 105363T), and Micromonospora luteiviridis sp. nov. type strain SGB14T (=CECT 9370T; =DSM 105362T).
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Monitoring the colonization and infection of legume nodules by Micromonospora in co-inoculation experiments with rhizobia
Scientific reports, 2017Co-Authors: P. Benito, Pablo Alonso-vega, Carolina Aguado, Rafael Luján, Yojiro Anzai, Ann M. Hirsch, Martha E TrujilloAbstract:The discovery that the actinobacterium Micromonospora inhabits nitrogen-fixing nodules raised questions as to its potential ecological role. The capacity of two Micromonospora strains to infect legumes other than their original host, Lupinus angustifolius, was investigated using Medicago and Trifolium as test plants. Compatible rhizobial strains were used for coinoculation of the plants because Micromonospora itself does not induce nodulation. Over 50% of nodules from each legume housed Micromonospora, and using 16S rRNA gene sequence identification, we verified that the reisolated strains corresponded to the microorganisms inoculated. Entry of the bacteria and colonization of the plant hosts were monitored using a GFP-tagged Lupac 08 mutant together with rhizobia, and by using immunogold labeling. Strain Lupac 08 was localized in plant tissues, confirming its capacity to enter and colonize all hosts. Based on studying three different plants, our results support a non-specific relationship between Micromonospora and legumes. Micromonospora Lupac 08, originally isolated from Lupinus re-enters root tissue, but only when coinoculated with the corresponding rhizobia. The ability of Micromonospora to infect and colonize different legume species and function as a potential plant-growth promoting bacterium is relevant because this microbe enhances the symbiosis without interfering with the host and its nodulating and nitrogen-fixing microbes.
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Micromonospora ureilytica sp. nov., Micromonospora noduli sp. nov. and Micromonospora vinacea sp. nov., isolated from Pisum sativum nodules.
International journal of systematic and evolutionary microbiology, 2016Co-Authors: Lorena Carro, Cathrin Spröer, Raúl Riesco, Martha E TrujilloAbstract:A diversity study on the presence of strains representing the genus Micromonospora in Pisum sativum nodules collected from Canizal (Spain) has provided evidence of the high number of isolates that might represent novel species. In the present work, we have characterized three of these isolates: GUI23T, GUI43T and GUI63T. Phenotypic and genotypic analyses confirmed that all strains represent novel species of the genus Micromonospora with the following proposed names: Micromonospora ureilytica sp. nov., type strain GUI23T (=CECT 9022T=DSM 101692T), Micromonospora noduli sp. nov., type strain GUI43T (=CECT 9020T=DSM 101694T), and Micromonospora vinacea sp. nov., type strain GUI63T (=CECT 9019T=DSM 101695T).
Somboon Tanasupawat - One of the best experts on this subject based on the ideXlab platform.
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Micromonospora radicis sp. nov., isolated from roots of Azadirachta indica var. siamensis Valenton, and reclassification of Jishengella zingiberis as Micromonospora zingiberis comb. nov.
International journal of systematic and evolutionary microbiology, 2019Co-Authors: Nattakorn Kuncharoen, Takuji Kudo, Pattama Pittayakhajonwut, Masahiro Yuki, Moriya Okuma, Somboon TanasupawatAbstract:A novel endophytic actinomycete strain AZ1-13T was isolated from roots of Azadirachta indica, and its taxonomic position was investigated using a polyphasic approach. Pairwise 16S rRNA gene sequence similarities of strain AZ1-13T and its closest species, Jishegella zingiberis PLAI1-1T and Micromonospora endophytica 202201T, were 99.7 and 99.2 %, respectively. Phylogenetic analyses of the family Micromonosporaceae based on 16S rRNA gene sequences indicated strains AZ1-13T and J. zingiberis PLAI1-1Tare located within the genus Micromonospora . The approximate genome size of the strain was 5.96 Mb with 71.9 mol% of G+C content. The strain AZ1-13T exhibited ANIb values of 87.4 % with J. zingiberis PLAI1-1T and 85.1 % with M. endophytica 202201T. Chemotaxonomic characteristics of strain AZ1-13T were consistent within the genus Micromonospora : cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid; glucose, mannose, ribose and xylose are presented as the whole-cell sugars; the predominant menaquinones were MK-9(H4) and MK-9(H6); major cellular fatty acids were iso-C15 : 0, 10-methyl C17 : 0, C17 : 0, anteiso-C17 : 0 and iso-C17 : 1ω8c; diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol were detected as distinguished phospholipids. Based on phenotypic properties, phylogeny and genomic data, the strain AZ1-13T could be distinguished from its closest neighbours, representing a novel species of the genus Micromonospora , for which the name Micromonospora radicis sp. nov. is proposed. The type strain is AZ1-13T (=KCTC 39786T=NBRC 112324T=JCM 32147T = TISTR 2404T). This study also proposed that J. zingiberis is transferred to the genus Micromonospora as Micromonospora zingiberis comb. nov. (type strain PLAI1-1T=TBRC 7644T=NBRC 113144T=JCM 32592T).
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Micromonospora caldifontis sp. nov., isolated from hot spring soil.
International journal of systematic and evolutionary microbiology, 2019Co-Authors: Chitti Thawai, Somboon Tanasupawat, Takuji KudoAbstract:A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil. The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora . The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphoglycolipid. The major menaquinones were MK-10(H6) and MK-10(H4). The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 70.5 mol%. 16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %). The physiological and DNA–DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species. Thus, the strain represents a novel species of the genus Micromonospora , for which the name Micromonospora caldifontis sp. nov. is proposed. The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
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Micromonospora azadirachtae sp. nov., isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton
Antonie van Leeuwenhoek, 2019Co-Authors: Nattakorn Kuncharoen, Moriya Ohkuma, Takuji Kudo, Somboon TanasupawatAbstract:A Gram-stain positive actinomycete, strain AZ1-19^T, isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton, collected from Chachoengsao province, Thailand, was characterised taxonomically by using a polyphasic approach. Strain AZ1-19^T was found to have characteristics consistent with those of members of the genus Micromonospora . The cell wall peptidoglycan of the strain was found to contain meso -diaminopimelic acid. The predominant phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The characteristic whole-cell sugars were identified as glucose, xylose, galactose and mannose. The major menaquinones were found to be MK-9(H_6), MK-10(H_6) and MK-10(H_8), and the major cellular fatty acids were identified as iso -C_16:0, iso -C_15:0, anteiso -C_15:0 and anteiso -C_17:0. Comparative analysis of 16S rRNA gene sequences revealed that strain AZ1-19^T is closely related to Micromonospora costi CS1-12^T (98.75% similarity), Micromonospora avicenniae 268506^T (98.75%), Micromonospora haikouensis 232617^T (98.68%) and Micromonospora siamensis TT2-4^T (98.61%), whilst the corresponding phylogenetic analysis based on partial gyrase subunit B ( gyr B) gene sequences indicated that strain AZ1-19^T forms a clade with M. avicenniae 268506^T with a high bootstrap value. The DNA G + C content was determined to be 69.8 mol%. Moreover, a combination of DNA–DNA relatedness values and some phenotypic and chemotaxonomic properties indicated that the strain could be distinguished from closely related species. Therefore, it is considered that strain AZ1-19^T represents a novel Micromonospora species for which the name Micromonospora azadirachtae sp. nov. is proposed. The type strain is AZ1-19^T (= KCTC 39941^T = NBRC 112784^T = JCM 32148^T = TISTR 2559^T).
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Micromonospora sediminis sp. nov., isolated from mangrove sediment.
International journal of systematic and evolutionary microbiology, 2016Co-Authors: Wongsakorn Phongsopitanun, Khanit Suwanborirux, Takuji Kudo, Pattama Pittayakhajonwut, Moriya Ohkuma, Somboon TanasupawatAbstract:The novel actinomycete, strain CH3-3T, was isolated from mangrove sediment collected from Chonburi Province, Thailand. On the basis of its spore morphology and chemotaxonomic characteristics, the strain belonged to the genus Micromonospora . It contained meso-diaminopimelic acid, glucose, mannose, xylose, ribose and rhamnose in the whole-cell hydrolysate, MK-10(H4), MK-10(H6) and MK-10(H8) as major menaquinones, and iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 as major cellular fatty acids. blast analysis of 16S rRNA gene sequences revealed that the strain showed highest similarity with Micromonospora palomenae NEAU-CX1T (98.97 %) and Micromonospora coxensis 2–30-b(28)T (98.97 %). Based on phylogenetic tree analysis of the 16S rRNA gene sequence, the strain formed a cluster with M. palomenae NEAU-CX1T, Micromonospora halophytica DSM 43171T, M. coxensis 2-30-b(28)T and Micromonospora purpureochromogenes DSM 43821T. On the basis of phenotypic differences and DNA–DNA relatedness evidence, strain CH3-3T could be clearly distinguished from the closely related species of the genus Micromonospora and represents a novel species of the genus Micromonospora for which the name Micromonospora sediminis sp. nov. is proposed. The type strain is CH3-3T (=JCM 18523T=PCU 350T=TISTR 2396T).
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Micromonospora fluostatini sp. nov., isolated from marine sediment.
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Wongsakorn Phongsopitanun, Khanit Suwanborirux, Takuji Kudo, Mihoko Mori, Kazuro Shiomi, Pattama Pittayakhajonwut, Somboon TanasupawatAbstract:The novel actinomycete strain PWB-003T, which produced fluostatins B and C antibiotics, was isolated from nearshore sediment collected from Panwa Cape, Phuket Province, Thailand. Data from the present polyphasic study indicated that strain PWB-003T represented a member of the genus Micromonospora. It produced single spores on substrate mycelia and contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. The predominant menaquinone was MK-10 (H4). Cellular fatty acids comprised C18 : 1ω9c, iso-C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0. On the basis of 16S rRNA gene sequence similarity analysis, the novel strain was closely related to Micromonospora eburnea LK2-10T (99.38 %), Micromonospora chaiyaphumensis MC5-1T (99.16 %), Micromonospora yangpuensis FXJ6.011T (98.97 %), Micromonospora echinaurantiaca DSM 43904T (98.97 %), Micromonospora pallida DSM 43817T (98.97 %), Micromonospora sagamiensis DSM 43912T and Micromonospora auratinigra JCM 12357T (both 98.97 %). The G+C content of the DNA was 74.5 mol%. DNA–DNA relatedness values among strain PWB-003T and related type strains ranged from 11.3 ± 1.3 to 38.8 ± 1.1 %. On the basis of these observations, strain PWB-003T could be distinguished from its closely related type strains and is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora fluostatini sp. nov. (type strain PWB-003T = JCM 30529T = PCU 341T = TISTR 2345T) is proposed.
Lorena Carro - One of the best experts on this subject based on the ideXlab platform.
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Micromonospora acroterricola sp nov a novel actinobacterium isolated from a high altitude atacama desert soil
International Journal of Systematic and Evolutionary Microbiology, 2019Co-Authors: Imen Nouioui, Lorena Carro, Patrycja Golinska, Hans-peter Klenk, Alan T. Bull, Jose M Igual, Barbara A Andrews, Michael GoodfellowAbstract:A Micromonospora strain, designated 5R2A7T, isolated from a high altitude Atacama Desert soil was examined by using a polyphasic approach. Strain 5R2A7T was found to have morphological, chemotaxonomic and cultural characteristics typical of members of the genus Micromonospora . The cell wall contains meso- and hydroxy-diaminopimelic acid, the major whole-cell sugars are glucose, ribose and xylose, the predominant menaquinones MK-10(H4), MK-10(H6), MK-10(H8) and MK-9(H6), the major polar lipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unknown glycolipid, and the predominant cellular fatty acids iso-C16 : 0, iso-C15 : 0 and 10-methyl C17 : 0. The digital genomic DNA G+C content is 72.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain 5R2A7T was closely related to Micromonospora coriariae DSM 44875T (99.8 %) and Micromonospora cremea CR30T (99.7 %), and was separated readily from the latter, its closest phylogenetic neighbour, based on gyrB and multilocus sequence data, by low average nucleotide identity (92.59 %) and in silico DNA–DNA relatedness (51.7 %) values calculated from draft genome assemblies and by a range of chemotaxonomic and phenotypic properties. Consequently, strain 5R2A7T is considered to represent a novel species of Micromonospora for which the name Micromonospora acroterricola sp. nov. is proposed. The type strain is 5R2A7T (=LMG 30755T=CECT 9656T).
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Micromonospora orduensis sp. nov., isolated from deep marine sediment
Antonie van Leeuwenhoek, 2019Co-Authors: Aysel Veyisoglu, Lorena Carro, Hans-peter Klenk, Jose M Igual, Demet Cetin, Nevzat SahinAbstract:A novel actinobacterial strain, designated S2509T, was isolated from marine sediment collected by a dredge at a depth of 45 m along Melet River offshore of the southern Black Sea coast, Ordu, Turkey. The cell wall peptidoglycan of strain was found to contain meso-diaminopimelic acid and 3-OH-diaminopimelic acid. The whole cell sugars detected were arabinose, glucose, rhamnose, ribose and xylose. The diagnostic phospholipids of strain S2509T were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, a glycolipid and two unidentified phospholipids. The predominant menaquinones were identified as MK-9(H8), MK-9(H6), MK-10(H8), MK-9(H4), MK-10(H4) and MK-10(H6). The major cellular fatty acids were found to be iso-C16:0, iso-C15:0 and 10-methyl C17:0. The taxonomic position of the strain was established using a polyphasic approach, showing that S2509T strain belongs to the genus Micromonospora. Phylogenetic analysis based on the 16S rRNA gene sequence of strain S2509T showed that it is closely related to the type strain of Micromonospora chokoriensis DSM 45160T (99.37% sequence similarity), and phylogenetically clustered with Micromonospora inaquosa LB39T (99.37%), Micromonospora lupini Lupac 14NT (99.16%), Micromonospora violae NEAU-zh8T (99.23%) and Micromonospora taraxaci NEAU-P5T (99.03%). The phylogenetic analysis based on the gyrB gene sequence of strain S2509T confirmed its close relationship with M. chokoriensis JCM 13247T (96.5% sequence similarity). Whole genome sequences confirmed by digital DNA-DNA hybridization analysis that the strain S2509T represents a novel species in the genus Micromonospora, for which the name Micromonospora orduensis sp. nov. is proposed. The type strain is S2509T (=DSM 45926T = KCTC 29201T).
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uncovering the potential of novel Micromonosporae isolated from an extreme hyper arid atacama desert soil
Scientific Reports, 2019Co-Authors: Valeria Razmilic, Che Pan, Imen Nouioui, Lorena Carro, Jean Franco Castro, Jose M IgualAbstract:The taxonomic status, biotechnological and ecological potential of several Micromonospora strains isolated from an extreme hyper arid Atacama Desert soil were determined. Initially, a polyphasic study was undertaken to clarify the taxonomic status of five Micromonosporae, strains LB4, LB19, LB32T, LB39T and LB41, isolated from an extreme hyper-arid soil collected from one of the driest regions of the Atacama Desert. All of the isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Micromonospora. Isolates LB32T and LB39T were distinguished from their nearest phylogenetic neighbours and proposed as new species, namely as Micromonospora arida sp. nov. and Micromonospora inaquosa sp. nov., respectively. Eluted methanol extracts of all of the isolates showed activity against a panel of bacterial and fungal indicator strains, notably against multi-drug resistant Klebsiella pneumoniae ATCC 700603 while isolates LB4 and LB41 showed pronounced anti-tumour activity against HepG2 cells. Draft genomes generated for the isolates revealed a rich source of novel biosynthetic gene clusters, some of which were unique to individual strains thereby opening up the prospect of selecting especially gifted Micromonosporae for natural product discovery. Key stress-related genes detected in the genomes of all of the isolates provided an insight into how Micromonosporae adapt to the harsh environmental conditions that prevail in extreme hyper-arid Atacama Desert soils.
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a study of three bacteria isolated from marine sediment and description of Micromonospora globispora sp nov
Systematic and Applied Microbiology, 2019Co-Authors: Lorena Carro, Hans-peter Klenk, Martha E Trujillo, Jose M Igual, Demet Cetin, Ayset Veyisoglu, Nevzat SahinAbstract:Abstract During a study looking for the isolation of new actinobacteria strains with potential for antibiotic production from deep marine sediment, three strains were collected with a morphology similar to the one described for the Micromonospora genus. A polyphasic study was designed to determine the taxonomic affiliation of the strains S2901T, S2903, and S2904. All the strains showed chemotaxonomic properties in line with their classification in the genus Micromonospora, meso-diaminopimelic acid in the wall peptidoglycan, a tetrahydrogenated menaquinone with nine isoprene units as major respiratory quinone, iso-C15:0 and iso-C16:0 as major fatty acids and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids. The 16S rRNA gene sequences of strain S2901T, S2903, and S2904 showed the highest similarity (99.2%) with the type strain of Micromonospora halophytica DSM 43171T, forming an independent branch in the phylogenetic gene tree. Their independent position was confirmed with gyrB gene and MLSA phylogenies. Whole genome sequences confirmed by digital DNA-DNA hybridization analysis that the isolates should be assigned to a new species within the genus Micromonospora for which the name Micromonospora globispora sp. nov. (S2901T, S2903 and S2904) is proposed.
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Hunting for cultivable Micromonospora strains in soils of the Atacama Desert
Antonie van Leeuwenhoek, 2018Co-Authors: Lorena Carro, Valeria Razmilic, Lee Richardson, Che Pan, Imen Nouioui, Patrycja Golinska, Hans-peter Klenk, Alan T. Bull, Juan A Asenjo, Michael GoodfellowAbstract:Innovative procedures were used to selectively isolate small numbers of Micromonospora strains from extreme hyper-arid and high altitude Atacama Desert soils. Micromonosporae were recognised on isolation plates by their ability to produce filamentous microcolonies that were strongly attached to the agar. Most of the isolates formed characteristic orange colonies that lacked aerial hyphae and turned black on spore formation, whereas those from the high altitude soil were dry, blue-green and covered by white aerial hyphae. The isolates were assigned to seven multi- and eleven single-membered groups based on BOX-PCR profiles. Representatives of the groups were assigned to either multi-membered clades that also contained marker strains or formed distinct phyletic lines in the Micromonospora 16S rRNA gene tree; many of the isolates were considered to be putatively novel species of Micromonospora. Most of the isolates from the high altitude soils showed activity against wild type strains of Bacillus subtilis and Pseudomonas fluorescens while those from the rhizosphere of Parastrephia quadrangulares and from the Lomas Bayas hyper-arid soil showed resistance to UV radiation.
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Micromonospora caldifontis sp. nov., isolated from hot spring soil.
International journal of systematic and evolutionary microbiology, 2019Co-Authors: Chitti Thawai, Somboon Tanasupawat, Takuji KudoAbstract:A single spore forming actinomycete, designated strain HSS6-8T, was isolated from a sample of hot spring soil. The strain had the chemotaxonomic properties consistent with its classification in the genus Micromonospora . The strain was found to have meso-diaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The reducing sugars in the cell hydrolysates were glucose, arabinose, xylose, ribose, mannose, galactose and rhamnose. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphoglycolipid. The major menaquinones were MK-10(H6) and MK-10(H4). The major cellular fatty acids were iso-C16 : 0, anteiso-C17 : 0, C17 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 70.5 mol%. 16S rRNA gene sequence analysis revealed that strain HSS6-8T was closely related to Micromonospora nigra DSM 43818T (98.2 %), Micromonospora eburnea DSM 44814T (98.2 %) and Micromonospora spongicola S3-1T (98.1 %). The physiological and DNA–DNA hybridization data allowed the differentiation of strain HSS6-8T from its related species. Thus, the strain represents a novel species of the genus Micromonospora , for which the name Micromonospora caldifontis sp. nov. is proposed. The type strain is HSS6-8T (=TBRC 8927T=JCM 17126T).
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Micromonospora soli sp. nov., isolated from rice rhizosphere soil.
Antonie van Leeuwenhoek, 2016Co-Authors: Chitti Thawai, Chokchai Kittiwongwattana, Dusanee Thanaboripat, Chamroon Laosinwattana, Prommart Koohakan, Nonglak ParinthawongAbstract:An actinomycete strain SL3-70T was isolated from a rice field and characterised using a polyphasic approach. The morphological and chemotaxonomical characteristics of strain SL3-70T indicate that it belongs to the genus Micromonospora. The phylogenetic analysis of the nearly complete 16S rRNA gene sequence revealed that strain SL3-70T is a member of the genus Micromonospora, and is closely related to Micromonospora echinaurantica DSM 43904T (99.1 % 16S rRNA gene sequence similarity) and Micromonospora kangleipakensis MBRL 34T (98.8 %). DNA–DNA relatedness between strain SL3-70T and its relatives ranged from 21.2 % ± 0.6 to 38.7 % ± 0.4. The results obtained from our study indicate that strain SL3-70T represents a novel species of the genus Micromonospora, for which the name Micromonospora soli sp. nov. is proposed. The type strain is SL3-70T (=BCC 67268T; =NBRC 110009T).
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Micromonospora oryzae sp. nov., isolated from roots of upland rice.
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Chokchai Kittiwongwattana, Dusanee Thanaboripat, Chamroon Laosinwattana, Prommart Koohakan, Nonglak Parinthawong, Chitti ThawaiAbstract:An actinomycete strain, designated CP2R9-1T, was isolated from root internal tissues of upland rice (Oryza sativa). Based on a polyphasic approach, strain CP2R9-1T was characterized as a member of the genus Micromonospora. meso-Diaminopimelic acid and 3-OH-diaminopimelic acid were present in the cell-wall peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholipids and four unidentified polar lipids. Predominant menaquinones were MK-9(H4), MK-9(H6) and MK-10(H4). Whole-cell sugars consisted of ribose, xylose, arabinose and glucose. Phylogenetic analysis of the nearly complete 16S rRNA gene sequence suggested that strain CP2R9-1T was closely related to Micromonospora haikouensis 232617T (99.32 % similarity), Micromonospora carbonacea DSM 43168T (99.18 %) and Micromonospora krabiensis MA-2T (99.16 %). Strain CP2R9-1T was distinct from its closest relatives based on low levels of DNA–DNA relatedness (21.3 ± 0.1–41.7 ± 0.7 %) and phenotypic differences. The results presented in this study showed that strain CP2R9-1T represents a novel species of the genus Micromonospora, for which the name Micromonospora oryzae sp. nov. is proposed. The type strain is CP2R9-1T ( = BCC 67266T = NBRC 110007T).
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Micromonospora costi sp nov isolated from a leaf of costus speciosus
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Chitti ThawaiAbstract:An endophytic actinobacterial strain, CS1-12T, was isolated from a leaf of Costus speciosus. Single spores were observed directly on the substrate mycelia. The cell-wall peptidoglycan of this strain exhibited meso-diaminopimelic acid and 3-OH-meso-diaminopimelic acid. Arabinose, glucose, ribose, xylose and rhamnose were detected as whole-cell sugars. The diagnostic phospholipids of this strain were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The predominant menaquinones were MK-10(H6), MK-10(H8), MK-9(H4) and MK-10(H4). The main components of the cellular fatty acids (>10 %) were iso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 72.8 mol%. 16S rRNA gene sequence analysis data showed that strain CS1-12T should be classified in the genus Micromonospora and that it is closely related to Micromonospora fulviviridis DSM 43906T (99.3 %). The physiological, biochemical and chemotaxonomic properties, including the DNA–DNA hybridization results, indicated that strain CS1-12T could be judged a novel species of the genus Micromonospora , for which the name Micromonospora costi sp. nov. is proposed. The type strain is CS1-12T ( = BCC 58124T = NBRC 109518T).
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Micromonospora spongicola sp. nov., an actinomycete isolated from a marine sponge in the Gulf of Thailand
The Journal of Antibiotics, 2013Co-Authors: Khomsan Supong, Pattama Pittayakhajonwut, Khanit Suwanborirux, Chanwit Suriyachadkun, Chitti ThawaiAbstract:An actinomycete strain, S3-1^T, was isolated from marine sponge sample collected from the Gulf of Thailand. The strain is aerobic, Gram-positive and produced single spores at the tip of the substrate mycelium. Strain S3-1^T contained meso -diaminopimelic acid in the peptidoglycan, whole-cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. The polar lipid profile of strain S3-1^T consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphoglycolipid and unknown polar lipids. Morphological and chemotaxonomic characteristics of the strain were identified as a member of the genus Micromonospora . Phylogenetic analysis based on 16S rRNA gene sequence analysis of the strain showed similarity to Micromonospora nigra DSM 43818^T (98.8%), Micromonospora yangpuensis FXJ6.011^T (98.7%) and Micromonospora narathiwatensis BTG4-1^T (98.6%). The DNA G+C content was 72.7 mol%. The phenotypic characteristics and DNA–DNA relatedness values supported that the classification of this strain as a novel species in the genus Micromonospora , for which the name Micromonospora spongicola sp. nov. (type strain S3-1^T =BCC 45595^T=NBRC 108779^T) is proposed.