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Donato Traversa - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic relationships of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) within the order Spirurida inferred using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis
Parasitology Research, 2008Co-Authors: Raffaella Iorio, Jan Šlapeta, Domenico Otranto, Barbara Paoletti, Annunziata Giangaspero, Donato TraversaAbstract:The present study investigated genetic variability within a population of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) affecting horses in an endemic area of central Italy using polymerase chain reaction (PCR)-coupled sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene ( cox1 ). No different cox1 sequences were detected in any of the H. muscae individual, while two haplotypes representing H. microstoma individuals differed for one substitution. The pairwise distance between the H. muscae and H. microstoma was 11%, coding for five amino acid changes. The sequence of an informative region within the cox1 gene of H. microstoma and H. muscae was analyzed by Maximum Likelihood and Bayesian phylogenetic methods using available mitochondrial sequences spirurid taxa belonging to Filarioidea, Thelazioidea, and Habronematoidea. Phylogenetic analysis supported the split of the tree into two sister spirurid groups, Habronematoidea and Filarioidea + Thelazioidea. The phylogenetic and evolutionary implications of Habronema with Filaroidea and Thelazioidea are discussed.
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identification of the intermediate hosts of habronema microstoma and habronema muscae under field conditions
Medical and Veterinary Entomology, 2008Co-Authors: Donato Traversa, Raffaella Iorio, Barbara Paoletti, D Otranto, Augusto Carluccio, Alberto Contri, Roberto Bartolini, Annunziata GiangasperoAbstract:A polymerase chain reaction (PCR)-based assay was used for the specific detection of Habronema microstoma and Habronema muscae (Nematoda, Spirurida) in order to identify the intermediate hosts of both nematode species under field conditions. A total of 1087 netted and 165 laboratory-bred flies were tested. Flies were identified as Musca domestica Linnaeus 1758, Musca autumnalis De Geer 1776, Haematobia irritans (Linnaeus 1758), Haematobia titillans (De Geer 1907) and Stomoxys calcitrans (Linnaeus 1758) (Muscidae). Genomic DNA was extracted from pools of fly heads, thoraces and abdomens, and 703 samples were subjected to a duplex two-step semi-nested PCR assay to specifically detect diagnostic regions within the ribosomal ITS2 sequence of both H. microstoma and H. muscae. Stomoxys calcitrans specimens were positive for H. microstoma DNA and M. domestica specimens were positive for H. muscae DNA. In particular, PCR-positive samples derived from both farm-netted and laboratory-bred flies. The present study represents the first evidence of the vectorial competence of different fly species as intermediate hosts of Habronema stomachworms under field conditions. We discuss the roles of S. calcitrans and M. domestica in transmitting H. microstoma and H. muscae.
Fumio Ohnishi - One of the best experts on this subject based on the ideXlab platform.
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a case of Microstomia subsequent to toxic epidermal necrolysis surgically treated by simple technique
Plastic and reconstructive surgery. Global open, 2013Co-Authors: Takanobu Mashiko, Toshiharu Minabe, Aiko Oka, Fumio OhnishiAbstract:Toxic epidermal necrolysis (TEN) is a rare and often fatal dermatitis, usually secondary to side effects of a drug.1 It is at the severe end of the spectrum of autoimmune conditions, such as Stevens-Johnson syndrome, and is defined as detachment of full-thickness epidermis involving more than 30% of the body surface area.2 The drugs commonly causing TEN are antibiotics such as sulfonamides and nonsteroidal anti-inflammatory drugs. TEN affects many parts of the body, though it most severely affects the mucous membranes including the mouth, oropharynx, conjunctiva, and vagina. With regard to the mouth, the membrane becomes blistered and eroded, making food ingestion difficult and sometimes necessitating tube feeding. Even after TEN is clinically healed, morbidity such as reduction of oral aperture due to mucosal scar contracture at the oral angles could remain as a state of Microstomia. Although many cases of congenital or postburn Microstomia were previously reported, there have been few reports of Microstomia caused by TEN, as far as we searched. Because clinical state of Microstomia subsequent to TEN is specifically localized to mucosal region, the therapeutic strategy should be newly established. Herein, we report a case of Microstomia subsequent to TEN, which was successfully restored by a simple surgical technique.
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A Case of Microstomia Subsequent to Toxic Epidermal Necrolysis Surgically Treated by Simple Technique
Wolters Kluwer, 2013Co-Authors: Takanobu Mashiko, Toshiharu Minabe, Aiko Oka, Fumio OhnishiAbstract:Summary: Toxic epidermal necrolysis (TEN) is a rare but severe adverse dermatitis that is an autoimmune reaction to drugs such as nonsteroidal anti-inflammatory drugs. TEN most severely affects the mucous membranes including the mouth and could develop into Microstomia; however, Microstomia in relation to TEN has rarely been reported in the literature. We describe an adult female patient who developed Microstomia due to scar contracture of the bilateral oral commissures subsequent to TEN and was successfully treated by a simple surgical technique consisting solely of transverse incision of the commissure and longitudinal closure
Raffaella Iorio - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic relationships of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) within the order Spirurida inferred using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis
Parasitology Research, 2008Co-Authors: Raffaella Iorio, Jan Šlapeta, Domenico Otranto, Barbara Paoletti, Annunziata Giangaspero, Donato TraversaAbstract:The present study investigated genetic variability within a population of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) affecting horses in an endemic area of central Italy using polymerase chain reaction (PCR)-coupled sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene ( cox1 ). No different cox1 sequences were detected in any of the H. muscae individual, while two haplotypes representing H. microstoma individuals differed for one substitution. The pairwise distance between the H. muscae and H. microstoma was 11%, coding for five amino acid changes. The sequence of an informative region within the cox1 gene of H. microstoma and H. muscae was analyzed by Maximum Likelihood and Bayesian phylogenetic methods using available mitochondrial sequences spirurid taxa belonging to Filarioidea, Thelazioidea, and Habronematoidea. Phylogenetic analysis supported the split of the tree into two sister spirurid groups, Habronematoidea and Filarioidea + Thelazioidea. The phylogenetic and evolutionary implications of Habronema with Filaroidea and Thelazioidea are discussed.
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identification of the intermediate hosts of habronema microstoma and habronema muscae under field conditions
Medical and Veterinary Entomology, 2008Co-Authors: Donato Traversa, Raffaella Iorio, Barbara Paoletti, D Otranto, Augusto Carluccio, Alberto Contri, Roberto Bartolini, Annunziata GiangasperoAbstract:A polymerase chain reaction (PCR)-based assay was used for the specific detection of Habronema microstoma and Habronema muscae (Nematoda, Spirurida) in order to identify the intermediate hosts of both nematode species under field conditions. A total of 1087 netted and 165 laboratory-bred flies were tested. Flies were identified as Musca domestica Linnaeus 1758, Musca autumnalis De Geer 1776, Haematobia irritans (Linnaeus 1758), Haematobia titillans (De Geer 1907) and Stomoxys calcitrans (Linnaeus 1758) (Muscidae). Genomic DNA was extracted from pools of fly heads, thoraces and abdomens, and 703 samples were subjected to a duplex two-step semi-nested PCR assay to specifically detect diagnostic regions within the ribosomal ITS2 sequence of both H. microstoma and H. muscae. Stomoxys calcitrans specimens were positive for H. microstoma DNA and M. domestica specimens were positive for H. muscae DNA. In particular, PCR-positive samples derived from both farm-netted and laboratory-bred flies. The present study represents the first evidence of the vectorial competence of different fly species as intermediate hosts of Habronema stomachworms under field conditions. We discuss the roles of S. calcitrans and M. domestica in transmitting H. microstoma and H. muscae.
Takanobu Mashiko - One of the best experts on this subject based on the ideXlab platform.
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a case of Microstomia subsequent to toxic epidermal necrolysis surgically treated by simple technique
Plastic and reconstructive surgery. Global open, 2013Co-Authors: Takanobu Mashiko, Toshiharu Minabe, Aiko Oka, Fumio OhnishiAbstract:Toxic epidermal necrolysis (TEN) is a rare and often fatal dermatitis, usually secondary to side effects of a drug.1 It is at the severe end of the spectrum of autoimmune conditions, such as Stevens-Johnson syndrome, and is defined as detachment of full-thickness epidermis involving more than 30% of the body surface area.2 The drugs commonly causing TEN are antibiotics such as sulfonamides and nonsteroidal anti-inflammatory drugs. TEN affects many parts of the body, though it most severely affects the mucous membranes including the mouth, oropharynx, conjunctiva, and vagina. With regard to the mouth, the membrane becomes blistered and eroded, making food ingestion difficult and sometimes necessitating tube feeding. Even after TEN is clinically healed, morbidity such as reduction of oral aperture due to mucosal scar contracture at the oral angles could remain as a state of Microstomia. Although many cases of congenital or postburn Microstomia were previously reported, there have been few reports of Microstomia caused by TEN, as far as we searched. Because clinical state of Microstomia subsequent to TEN is specifically localized to mucosal region, the therapeutic strategy should be newly established. Herein, we report a case of Microstomia subsequent to TEN, which was successfully restored by a simple surgical technique.
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A Case of Microstomia Subsequent to Toxic Epidermal Necrolysis Surgically Treated by Simple Technique
Wolters Kluwer, 2013Co-Authors: Takanobu Mashiko, Toshiharu Minabe, Aiko Oka, Fumio OhnishiAbstract:Summary: Toxic epidermal necrolysis (TEN) is a rare but severe adverse dermatitis that is an autoimmune reaction to drugs such as nonsteroidal anti-inflammatory drugs. TEN most severely affects the mucous membranes including the mouth and could develop into Microstomia; however, Microstomia in relation to TEN has rarely been reported in the literature. We describe an adult female patient who developed Microstomia due to scar contracture of the bilateral oral commissures subsequent to TEN and was successfully treated by a simple surgical technique consisting solely of transverse incision of the commissure and longitudinal closure
Annunziata Giangaspero - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic relationships of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) within the order Spirurida inferred using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis
Parasitology Research, 2008Co-Authors: Raffaella Iorio, Jan Šlapeta, Domenico Otranto, Barbara Paoletti, Annunziata Giangaspero, Donato TraversaAbstract:The present study investigated genetic variability within a population of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) affecting horses in an endemic area of central Italy using polymerase chain reaction (PCR)-coupled sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene ( cox1 ). No different cox1 sequences were detected in any of the H. muscae individual, while two haplotypes representing H. microstoma individuals differed for one substitution. The pairwise distance between the H. muscae and H. microstoma was 11%, coding for five amino acid changes. The sequence of an informative region within the cox1 gene of H. microstoma and H. muscae was analyzed by Maximum Likelihood and Bayesian phylogenetic methods using available mitochondrial sequences spirurid taxa belonging to Filarioidea, Thelazioidea, and Habronematoidea. Phylogenetic analysis supported the split of the tree into two sister spirurid groups, Habronematoidea and Filarioidea + Thelazioidea. The phylogenetic and evolutionary implications of Habronema with Filaroidea and Thelazioidea are discussed.
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identification of the intermediate hosts of habronema microstoma and habronema muscae under field conditions
Medical and Veterinary Entomology, 2008Co-Authors: Donato Traversa, Raffaella Iorio, Barbara Paoletti, D Otranto, Augusto Carluccio, Alberto Contri, Roberto Bartolini, Annunziata GiangasperoAbstract:A polymerase chain reaction (PCR)-based assay was used for the specific detection of Habronema microstoma and Habronema muscae (Nematoda, Spirurida) in order to identify the intermediate hosts of both nematode species under field conditions. A total of 1087 netted and 165 laboratory-bred flies were tested. Flies were identified as Musca domestica Linnaeus 1758, Musca autumnalis De Geer 1776, Haematobia irritans (Linnaeus 1758), Haematobia titillans (De Geer 1907) and Stomoxys calcitrans (Linnaeus 1758) (Muscidae). Genomic DNA was extracted from pools of fly heads, thoraces and abdomens, and 703 samples were subjected to a duplex two-step semi-nested PCR assay to specifically detect diagnostic regions within the ribosomal ITS2 sequence of both H. microstoma and H. muscae. Stomoxys calcitrans specimens were positive for H. microstoma DNA and M. domestica specimens were positive for H. muscae DNA. In particular, PCR-positive samples derived from both farm-netted and laboratory-bred flies. The present study represents the first evidence of the vectorial competence of different fly species as intermediate hosts of Habronema stomachworms under field conditions. We discuss the roles of S. calcitrans and M. domestica in transmitting H. microstoma and H. muscae.