The Experts below are selected from a list of 21165 Experts worldwide ranked by ideXlab platform
Utku Baran - One of the best experts on this subject based on the ideXlab platform.
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in vivo imaging of microvascular changes in inflammatory human skin induced by tape stripping and mosquito saliva using optical microangiography
Proceedings of SPIE, 2015Co-Authors: Utku Baran, Woo June Choi, Ruikang K WangAbstract:Tape stripping on human skin induces mechanical disruptions of the epidermal barrier that lead to minor skin inflammation which leads to temporary changes in Microvasculature. On the other hand, when mosquitoes probe the skin for blood feeding, they inject saliva in dermal tissue. Mosquito saliva is known to exert various biological activities, such as dermal mast cell degranulation, leading to fluid extravasation and neutrophil influx. This inflammatory response remain longer than the tape stripping caused inflammation. In this study, we demonstrate the capabilities of swept-source optical coherence tomography (OCT) in detecting in vivo microvascular response of inflammatory human skin. Optical microangiography (OMAG), noninvasive volumetric Microvasculature in vivo imaging method, has been used to track the vascular responses after tape stripping and mosquito bite. Vessel density has been quantified and used to correlate with the degree of skin irritation. The proved capability of OMAG technique in visualizing the Microvasculature network under inflamed skin condition can play an important role in clinical trials of treatment and diagnosis of inflammatory skin disorders as well as studying mosquito bite’s perception by the immune system and its role in parasite transmission.
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in vivo blood flow imaging of inflammatory human skin induced by tape stripping using optical microangiography
Journal of Biophotonics, 2015Co-Authors: Hequn Wang, Utku BaranAbstract:Vasculature response is a hallmark for most inflammatory skin disorders. Tape stripping on human skin causes a minor inflammation which leads to changes in Microvasculature. In this study, optical microangiography (OMAG), noninvasive volumetric Microvasculature in vivo imaging method, has been used to track the vascular responses after tape stripping. Vessel density has been quantified and used to correlate with the degree of skin irritation. The proved capability of OMAG technique in visualizing the Microvasculature network under inflamed skin condition can play an important role in clinical trials of treatment and diagnosis of inflammatory skin disorders.
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in vivo blood flow imaging of inflammatory human skin induced by tape stripping using optical microangiography
Journal of Biophotonics, 2015Co-Authors: Hequn Wang, Utku BaranAbstract:Vasculature response is a hallmark for most inflammatory skin disorders. Tape stripping on human skin causes a minor inflammation which leads to changes in Microvasculature. In this study, optical microangiography (OMAG), noninvasive volumetric Microvasculature in vivo imaging method, has been used to track the vascular responses after tape stripping. Vessel density has been quantified and used to correlate with the degree of skin irritation. The proved capability of OMAG technique in visualizing the Microvasculature network under inflamed skin condition can play an important role in clinical trials of treatment and diagnosis of inflammatory skin disorders. (© 2015 WILEY-VCH Verlag GmbH &Co. KGaA, Weinheim)
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microvascular changes during acne lesion initiation and scarring is revealed in vivo using optical microangiography
Proceedings of SPIE, 2015Co-Authors: Utku Baran, Woo June ChoiAbstract:Acne is a common skin disease in society and often leads to scarring. In this paper, we demonstrate the capabilities of swept-source optical coherence tomography (SS-OCT) in detecting specific features of acne lesion initiation and scarring on human facial skin in vivo over 30 days. Optical microangiography (OMAG) technique made it possible to image 3D tissue Microvasculature changes up to 1 mm depth in vivo without the need of exogenous contrast agents in ~10 seconds. The presented results show promise to facilitate clinical trials of treatment and prognosis of acne vulgaris by detecting cutaneous Microvasculature and structural changes within human skin in vivo .
Thomas Boland - One of the best experts on this subject based on the ideXlab platform.
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human Microvasculature fabrication using thermal inkjet printing technology
Biomaterials, 2009Co-Authors: Xiaofeng Cui, Thomas BolandAbstract:The current tissue engineering paradigm is that successfully engineered thick tissues must include vasculature. As biological approaches alone, such as VEGF, have fallen short of their promises, one may look for an engineering approach to build Microvasculature. Layer-by-layer approaches for customized fabrication of cell/scaffold constructs have shown some potential in building complex 3D structures. With the advent of cell printing, one may be able to build precise human Microvasculature with suitable bio-ink. Human microvascular endothelial cells (HMVEC) and fibrin were studied as bio-ink for Microvasculature construction. Endothelial cells are the only cells to compose the human capillaries and also form the entire inner lining of cardiovascular system. Fibrin has been already widely recognized as tissue engineering scaffold for vasculature and other cells, including skeleton/smooth muscle cells and chondrocytes. In our study, we precisely fabricated micron-sized fibrin channels using a drop-on-demand polymerization. This printing technique uses aqueous processes that have been shown to induce little, if any, damage to cells. When printing HMVEC cells in conjunction with the fibrin, we found the cells aligned themselves inside the channels and proliferated to form confluent linings. The 3D tubular structure was also found in the printed patterns. We conclude that a combined simultaneous cell and scaffold printing can promote HMVEC proliferation and Microvasculature formation.
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Human Microvasculature fabrication using thermal inkjet printing technology
Biomaterials, 2009Co-Authors: Xiaofeng Cui, Thomas BolandAbstract:The current tissue engineering paradigm is that successfully engineered thick tissues must include vasculature. As biological approaches alone, such as VEGF, have fallen short of their promises, one may look for an engineering approach to build Microvasculature. Layer-by-layer approaches for customized fabrication of cell/scaffold constructs have shown some potential in building complex 3D structures. With the advent of cell printing, one may be able to build precise human Microvasculature with suitable bio-ink. Human microvascular endothelial cells (HMVEC) and fibrin were studied as bio-ink for Microvasculature construction. Endothelial cells are the only cells to compose the human capillaries and also form the entire inner lining of cardiovascular system. Fibrin has been already widely recognized as tissue engineering scaffold for vasculature and other cells, including skeleton/smooth muscle cells and chondrocytes. In our study, we precisely fabricated micron-sized fibrin channels using a drop-on-demand polymerization. This printing technique uses aqueous processes that have been shown to induce little, if any, damage to cells. When printing HMVEC cells in conjunction with the fibrin, we found the cells aligned themselves inside the channels and proliferated to form confluent linings. The 3D tubular structure was also found in the printed patterns. We conclude that a combined simultaneous cell and scaffold printing can promote HMVEC proliferation and Microvasculature formation. ?? 2009 Elsevier Ltd. All rights reserved.
Min Hee Suh - One of the best experts on this subject based on the ideXlab platform.
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Optical coherence tomography angiography (OCTA) Microvasculature images of optic nerve head in glaucoma eye without Microvasculature dropout inside the optic disc.
2018Co-Authors: Tadamichi Akagi, Linda M. Zangwill, Min Hee Suh, Patricia Isabel C. Manalastas, Adeleh Yarmohammadi, Luke J. Saunders, Takuhei Shoji, Rafaella C. Penteado, Robert N. WeinrebAbstract:OCTA en face projection images in the retinal nerve fiber layer (RNFL) (Top left), superficial layer (Top right), and the whole-depth (Second row). The red ellipse indicates the optic disc boundary and red lines show the boundaries of evaluation areas. Superotemporal and inferotemporal regions were used for evaluation of Microvasculature dropout. The yellow and green lines indicate the location of the B-scans in the bottom row. Bottom, Cross-sectional angiogram images overlying the B-scan images. Microvasculature in the anterior portion of lamina cribrosa is visualized in the area shown by orange arrowheads, while the anterior lamina cribrosa and Microvasculature cannot be detected because of vessel shadowing in the area shown by aqua arrowheads. Yellow and aqua borders show the inner limiting membrane (ILM) and 80 μm below the ILM, respectively.
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deep retinal layer Microvasculature dropout detected by the optical coherence tomography angiography in glaucoma
Ophthalmology, 2016Co-Authors: Linda M. Zangwill, Min Hee Suh, Patricia Isabel C. Manalastas, Akram Belghith, Adeleh Yarmohammadi, Luke J. Saunders, Felipe A Medeiros, Alberto Dinizfilho, Robert N. WeinrebAbstract:Purpose To investigate factors associated with dropout of the parapapillary deep retinal layer Microvasculature assessed by optical coherence tomography angiography (OCTA) in glaucomatous eyes. Design Cross-sectional study. Participants Seventy-one eyes from 71 primary open-angle glaucoma (POAG) patients with β-zone parapapillary atrophy (βPPA) enrolled in the Diagnostic Innovations in Glaucoma Study. Methods Parapapillary deep-layer Microvasculature dropout was defined as a complete loss of the Microvasculature located within the deep retinal layer of the βPPA from OCTA-derived optic nerve head vessel density maps by standardized qualitative assessment. Circumpapillary vessel density (cpVD) within the retinal nerve fiber layer (RNFL) also was calculated using OCTA. Choroidal thickness and presence of focal lamina cribrosa (LC) defects were determined using swept-source optical coherence tomography. Main Outcome Measures Presence of parapapillary deep-layer Microvasculature dropout. Parameters including age, systolic and diastolic blood pressure, axial length, intraocular pressure, disc hemorrhage, cpVD, visual field (VF) mean deviation (MD), focal LC defects βPPA area, and choroidal thickness were analyzed. Results Parapapillary deep-layer Microvasculature dropout was detected in 37 POAG eyes (52.1%). Eyes with Microvasculature dropout had a higher prevalence of LC defects (70.3% vs. 32.4%), lower cpVD (52.7% vs. 58.8%), worse VF MD (−9.06 dB vs. −3.83 dB), thinner total choroidal thickness (126.5 μm vs. 169.1 μm), longer axial length (24.7 mm vs. 24.0 mm), larger βPPA (1.2 mm 2 vs. 0.76 mm 2 ), and lower diastolic blood pressure (74.7 mmHg vs. 81.7 mmHg) than those without dropout ( P P = 0.012), reduced cpVD (OR, 1.27; P = 0.002), worse VF MD (OR, 1.27; P = 0.001), thinner choroidal thickness (OR, 1.02; P = 0.014), and lower diastolic blood pressure (OR, 1.16; P = 0.003) were associated significantly with the dropout. Conclusions Systemic and ocular factors including focal LC defects more advanced glaucoma, reduced RNFL vessel density, thinner choroidal thickness, and lower diastolic blood pressure were factors associated with the parapapillary deep-layer Microvasculature dropout in glaucomatous eyes. Longitudinal studies are required to elucidate the temporal relationship between parapapillary deep-layer Microvasculature dropout and systemic and ocular factors.
Robert N. Weinreb - One of the best experts on this subject based on the ideXlab platform.
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Optical coherence tomography angiography (OCTA) Microvasculature images of optic nerve head in glaucoma eye without Microvasculature dropout inside the optic disc.
2018Co-Authors: Tadamichi Akagi, Linda M. Zangwill, Min Hee Suh, Patricia Isabel C. Manalastas, Adeleh Yarmohammadi, Luke J. Saunders, Takuhei Shoji, Rafaella C. Penteado, Robert N. WeinrebAbstract:OCTA en face projection images in the retinal nerve fiber layer (RNFL) (Top left), superficial layer (Top right), and the whole-depth (Second row). The red ellipse indicates the optic disc boundary and red lines show the boundaries of evaluation areas. Superotemporal and inferotemporal regions were used for evaluation of Microvasculature dropout. The yellow and green lines indicate the location of the B-scans in the bottom row. Bottom, Cross-sectional angiogram images overlying the B-scan images. Microvasculature in the anterior portion of lamina cribrosa is visualized in the area shown by orange arrowheads, while the anterior lamina cribrosa and Microvasculature cannot be detected because of vessel shadowing in the area shown by aqua arrowheads. Yellow and aqua borders show the inner limiting membrane (ILM) and 80 μm below the ILM, respectively.
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deep retinal layer Microvasculature dropout detected by the optical coherence tomography angiography in glaucoma
Ophthalmology, 2016Co-Authors: Linda M. Zangwill, Min Hee Suh, Patricia Isabel C. Manalastas, Akram Belghith, Adeleh Yarmohammadi, Luke J. Saunders, Felipe A Medeiros, Alberto Dinizfilho, Robert N. WeinrebAbstract:Purpose To investigate factors associated with dropout of the parapapillary deep retinal layer Microvasculature assessed by optical coherence tomography angiography (OCTA) in glaucomatous eyes. Design Cross-sectional study. Participants Seventy-one eyes from 71 primary open-angle glaucoma (POAG) patients with β-zone parapapillary atrophy (βPPA) enrolled in the Diagnostic Innovations in Glaucoma Study. Methods Parapapillary deep-layer Microvasculature dropout was defined as a complete loss of the Microvasculature located within the deep retinal layer of the βPPA from OCTA-derived optic nerve head vessel density maps by standardized qualitative assessment. Circumpapillary vessel density (cpVD) within the retinal nerve fiber layer (RNFL) also was calculated using OCTA. Choroidal thickness and presence of focal lamina cribrosa (LC) defects were determined using swept-source optical coherence tomography. Main Outcome Measures Presence of parapapillary deep-layer Microvasculature dropout. Parameters including age, systolic and diastolic blood pressure, axial length, intraocular pressure, disc hemorrhage, cpVD, visual field (VF) mean deviation (MD), focal LC defects βPPA area, and choroidal thickness were analyzed. Results Parapapillary deep-layer Microvasculature dropout was detected in 37 POAG eyes (52.1%). Eyes with Microvasculature dropout had a higher prevalence of LC defects (70.3% vs. 32.4%), lower cpVD (52.7% vs. 58.8%), worse VF MD (−9.06 dB vs. −3.83 dB), thinner total choroidal thickness (126.5 μm vs. 169.1 μm), longer axial length (24.7 mm vs. 24.0 mm), larger βPPA (1.2 mm 2 vs. 0.76 mm 2 ), and lower diastolic blood pressure (74.7 mmHg vs. 81.7 mmHg) than those without dropout ( P P = 0.012), reduced cpVD (OR, 1.27; P = 0.002), worse VF MD (OR, 1.27; P = 0.001), thinner choroidal thickness (OR, 1.02; P = 0.014), and lower diastolic blood pressure (OR, 1.16; P = 0.003) were associated significantly with the dropout. Conclusions Systemic and ocular factors including focal LC defects more advanced glaucoma, reduced RNFL vessel density, thinner choroidal thickness, and lower diastolic blood pressure were factors associated with the parapapillary deep-layer Microvasculature dropout in glaucomatous eyes. Longitudinal studies are required to elucidate the temporal relationship between parapapillary deep-layer Microvasculature dropout and systemic and ocular factors.
Xiaofeng Cui - One of the best experts on this subject based on the ideXlab platform.
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human Microvasculature fabrication using thermal inkjet printing technology
Biomaterials, 2009Co-Authors: Xiaofeng Cui, Thomas BolandAbstract:The current tissue engineering paradigm is that successfully engineered thick tissues must include vasculature. As biological approaches alone, such as VEGF, have fallen short of their promises, one may look for an engineering approach to build Microvasculature. Layer-by-layer approaches for customized fabrication of cell/scaffold constructs have shown some potential in building complex 3D structures. With the advent of cell printing, one may be able to build precise human Microvasculature with suitable bio-ink. Human microvascular endothelial cells (HMVEC) and fibrin were studied as bio-ink for Microvasculature construction. Endothelial cells are the only cells to compose the human capillaries and also form the entire inner lining of cardiovascular system. Fibrin has been already widely recognized as tissue engineering scaffold for vasculature and other cells, including skeleton/smooth muscle cells and chondrocytes. In our study, we precisely fabricated micron-sized fibrin channels using a drop-on-demand polymerization. This printing technique uses aqueous processes that have been shown to induce little, if any, damage to cells. When printing HMVEC cells in conjunction with the fibrin, we found the cells aligned themselves inside the channels and proliferated to form confluent linings. The 3D tubular structure was also found in the printed patterns. We conclude that a combined simultaneous cell and scaffold printing can promote HMVEC proliferation and Microvasculature formation.
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Human Microvasculature fabrication using thermal inkjet printing technology
Biomaterials, 2009Co-Authors: Xiaofeng Cui, Thomas BolandAbstract:The current tissue engineering paradigm is that successfully engineered thick tissues must include vasculature. As biological approaches alone, such as VEGF, have fallen short of their promises, one may look for an engineering approach to build Microvasculature. Layer-by-layer approaches for customized fabrication of cell/scaffold constructs have shown some potential in building complex 3D structures. With the advent of cell printing, one may be able to build precise human Microvasculature with suitable bio-ink. Human microvascular endothelial cells (HMVEC) and fibrin were studied as bio-ink for Microvasculature construction. Endothelial cells are the only cells to compose the human capillaries and also form the entire inner lining of cardiovascular system. Fibrin has been already widely recognized as tissue engineering scaffold for vasculature and other cells, including skeleton/smooth muscle cells and chondrocytes. In our study, we precisely fabricated micron-sized fibrin channels using a drop-on-demand polymerization. This printing technique uses aqueous processes that have been shown to induce little, if any, damage to cells. When printing HMVEC cells in conjunction with the fibrin, we found the cells aligned themselves inside the channels and proliferated to form confluent linings. The 3D tubular structure was also found in the printed patterns. We conclude that a combined simultaneous cell and scaffold printing can promote HMVEC proliferation and Microvasculature formation. ?? 2009 Elsevier Ltd. All rights reserved.
