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Ruddy Richard - One of the best experts on this subject based on the ideXlab platform.
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergström technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergström procedure on Mitochondrial Respiration in skeletal muscle. Methods 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergström [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration ( V _GM-ADP) was assessed using an oxygraphic method on permeabilized fibers. Results The weight of the muscle samples and V _GM-ADP decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration ( r = 0.99, p
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European journal of applied physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle.
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Microbiopsies versus Bergstrom needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle. 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergstrom [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration (V (GM-ADP)) was assessed using an oxygraphic method on permeabilized fibers. The weight of the muscle samples and V (GM-ADP) decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration (r = 0.99, p < 0.05) and between needle size and maximal Mitochondrial Respiration (r = 0.99, p < 0.05). Microbiopsies give lower muscle sample weight and maximal rate of Mitochondrial Respiration compared to the standard Bergstrom needle.Therefore, the higher the gauge (i.e. the smaller the size) of the microbiopsy needle, the lower is the maximal rate of Respiration. Microbiopsies of skeletal muscle underestimate the maximal Mitochondrial Respiration rate, and this finding needs to be highlighted for adequate interpretation and comparison with literature data.
M. E. Isner-horobeti - One of the best experts on this subject based on the ideXlab platform.
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergström technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergström procedure on Mitochondrial Respiration in skeletal muscle. Methods 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergström [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration ( V _GM-ADP) was assessed using an oxygraphic method on permeabilized fibers. Results The weight of the muscle samples and V _GM-ADP decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration ( r = 0.99, p
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European journal of applied physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle.
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Microbiopsies versus Bergstrom needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle. 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergstrom [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration (V (GM-ADP)) was assessed using an oxygraphic method on permeabilized fibers. The weight of the muscle samples and V (GM-ADP) decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration (r = 0.99, p < 0.05) and between needle size and maximal Mitochondrial Respiration (r = 0.99, p < 0.05). Microbiopsies give lower muscle sample weight and maximal rate of Mitochondrial Respiration compared to the standard Bergstrom needle.Therefore, the higher the gauge (i.e. the smaller the size) of the microbiopsy needle, the lower is the maximal rate of Respiration. Microbiopsies of skeletal muscle underestimate the maximal Mitochondrial Respiration rate, and this finding needs to be highlighted for adequate interpretation and comparison with literature data.
B. Geny - One of the best experts on this subject based on the ideXlab platform.
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Aging Exacerbates Ischemia-Reperfusion-Induced Mitochondrial Respiration Impairment in Skeletal Muscle
Antioxidants, 2019Co-Authors: Stéphanie Paradis, Daniel Metzger, Anne-laure Charles, Isabelle Georg, Fabienne Goupilleau, Alain Meyer, Michel Kindo, Gilles Laverny, B. GenyAbstract:Cycles of ischemia-reperfusion (IR) that occur during peripheral arterial disease (PAD) are associated with significant morbi-mortality, and aging is an irreversible risk factor of PAD. However, the effects of advanced age on IR-induced skeletal muscle Mitochondrial dysfunction are not well known. Young and aged mice were therefore submitted to hindlimb IR (2 h ischemia followed by 2 h reperfusion). Skeletal muscle Mitochondrial Respiration, calcium retention capacity (CRC) and reactive oxygen species (ROS) production were determined using high resolution respirometry, spectrofluorometry and electronic paramagnetic resonance. IR-induced impairment in Mitochondrial Respiration was enhanced in old animals (V ADP ; from 33.0 ± 2.4 to 18.4 ± 3.8 and 32.8 ± 1.3 to 5.9 ± 2.7 pmol/s/mg wet weight; −44.2 ± 11.4% vs. −82.0 ± 8.1%, in young and aged mice, respectively). Baseline CRC was lower in old animals and IR similarly decreased the CRC in both groups (from 11.8 ± 0.9 to 4.6 ± 0.9 and 5.5 ± 0.9 to 2.1 ± 0.3 µmol/mg dry weight; −60.9 ± 7.3 and −60.9 ± 4.6%, in young and aged mice, respectively). Further, IR-induced ROS production tended to be higher in aged mice. In conclusion, aging exacerbated the deleterious effects of IR on skeletal muscle Mitochondrial Respiration, potentially in relation to an increased oxidative stress.
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergström technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergström procedure on Mitochondrial Respiration in skeletal muscle. Methods 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergström [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration ( V _GM-ADP) was assessed using an oxygraphic method on permeabilized fibers. Results The weight of the muscle samples and V _GM-ADP decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration ( r = 0.99, p
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European journal of applied physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle.
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Microbiopsies versus Bergstrom needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle. 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergstrom [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration (V (GM-ADP)) was assessed using an oxygraphic method on permeabilized fibers. The weight of the muscle samples and V (GM-ADP) decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration (r = 0.99, p < 0.05) and between needle size and maximal Mitochondrial Respiration (r = 0.99, p < 0.05). Microbiopsies give lower muscle sample weight and maximal rate of Mitochondrial Respiration compared to the standard Bergstrom needle.Therefore, the higher the gauge (i.e. the smaller the size) of the microbiopsy needle, the lower is the maximal rate of Respiration. Microbiopsies of skeletal muscle underestimate the maximal Mitochondrial Respiration rate, and this finding needs to be highlighted for adequate interpretation and comparison with literature data.
F. Daussin - One of the best experts on this subject based on the ideXlab platform.
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergström technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergström procedure on Mitochondrial Respiration in skeletal muscle. Methods 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergström [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration ( V _GM-ADP) was assessed using an oxygraphic method on permeabilized fibers. Results The weight of the muscle samples and V _GM-ADP decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration ( r = 0.99, p
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European journal of applied physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle.
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Microbiopsies versus Bergstrom needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle. 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergstrom [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration (V (GM-ADP)) was assessed using an oxygraphic method on permeabilized fibers. The weight of the muscle samples and V (GM-ADP) decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration (r = 0.99, p < 0.05) and between needle size and maximal Mitochondrial Respiration (r = 0.99, p < 0.05). Microbiopsies give lower muscle sample weight and maximal rate of Mitochondrial Respiration compared to the standard Bergstrom needle.Therefore, the higher the gauge (i.e. the smaller the size) of the microbiopsy needle, the lower is the maximal rate of Respiration. Microbiopsies of skeletal muscle underestimate the maximal Mitochondrial Respiration rate, and this finding needs to be highlighted for adequate interpretation and comparison with literature data.
A. Charton - One of the best experts on this subject based on the ideXlab platform.
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergström technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergström procedure on Mitochondrial Respiration in skeletal muscle. Methods 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergström [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration ( V _GM-ADP) was assessed using an oxygraphic method on permeabilized fibers. Results The weight of the muscle samples and V _GM-ADP decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration ( r = 0.99, p
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Microbiopsies versus Bergström needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European journal of applied physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Introduction Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle.
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Microbiopsies versus Bergstrom needle for skeletal muscle sampling: impact on maximal Mitochondrial Respiration rate
European Journal of Applied Physiology, 2014Co-Authors: M. E. Isner-horobeti, A. Charton, F. Daussin, B. Geny, S. P. Dufour, Ruddy RichardAbstract:Microbiopsies are increasingly used as an alternative to the standard Bergstrom technique for skeletal muscle sampling. The potential impact of these two different procedures on Mitochondrial Respiration rate is unknown. The objective of this work was to compare microbiopsies versus Bergstrom procedure on Mitochondrial Respiration in skeletal muscle. 52 vastus lateralis muscle samples were obtained from 13 anesthetized pigs, either with a Bergstrom [6 gauges (G)] needle or with microbiopsy needles (12, 14, 18G). Maximal Mitochondrial Respiration (V (GM-ADP)) was assessed using an oxygraphic method on permeabilized fibers. The weight of the muscle samples and V (GM-ADP) decreased with the increasing gauge of the needles. A positive nonlinear relationship was observed between the weight of the muscle sample and the level of maximal Mitochondrial Respiration (r = 0.99, p < 0.05) and between needle size and maximal Mitochondrial Respiration (r = 0.99, p < 0.05). Microbiopsies give lower muscle sample weight and maximal rate of Mitochondrial Respiration compared to the standard Bergstrom needle.Therefore, the higher the gauge (i.e. the smaller the size) of the microbiopsy needle, the lower is the maximal rate of Respiration. Microbiopsies of skeletal muscle underestimate the maximal Mitochondrial Respiration rate, and this finding needs to be highlighted for adequate interpretation and comparison with literature data.