The Experts below are selected from a list of 69 Experts worldwide ranked by ideXlab platform

R. M. R. Taylor - One of the best experts on this subject based on the ideXlab platform.

  • Renal allograft rejection: expression and function of VCAM‐1 on tubular epithelial cells
    Clinical and experimental immunology, 2008
    Co-Authors: Y. Lin, John A. Kirby, Da Browell, A. R. Morley, Brian K. Shenton, George Proud, R. M. R. Taylor
    Abstract:

    The interaction between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) is known to play an important role in stabilizing the adhesion of lymphocytes to endothelial cells. Such cellular adhesion is crucial to many immunological processes including lymphocyte-mediated cell lysis. In this study the expression of VCAM-1 on renal tubular epithelial cells is demonstrated on biopsy sections recovered during acute renal allograft rejection. Experiments performed using epithelial cells Cultured from renal tubules show that VCAM-1 is up-regulated by addition of the inflammatory cytokines tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). Combination of TNF-alpha and IFN-gamma synergized to induce high levels of VCAM-1 expression. Further experiments demonstrated that the cytokines produced by activated lymphocytes in Mixed Leucocyte Culture also up-regulate expression of VCAM-1. Assays of the adhesion of lymphoid cells to Cultured renal epithelial cells showed that cytokine pretreatment of the renal cells enhanced the binding of lymphoid cells. The proportion of bound lymphoid cells was significantly reduced by addition of an antibody capable of blocking the interaction of VCAM-1 with VLA-4. This result indicated that the VCAM-1 induced on renal epithelial cells by inflammatory cytokines is functionally capable of binding VLA-4, thereby enhancing the adhesion of potentially graft-damaging lymphoid cells.

L. U. Lamm - One of the best experts on this subject based on the ideXlab platform.

  • MLC — A Micro‐Modification of the Mixed Leucocyte Culture Technique
    Tissue Antigens, 2008
    Co-Authors: F. Jørgensen, L. U. Lamm
    Abstract:

    A micro-modification of the MLC technique is described, using 5 × 104 responding cells per single Culture in round bottomed microtiter plates. Over a wide range, there is a linear relation between the number of cells grown per well and the obtained response; and with the number of responding cells chosen, there is a linearity between log dose stimulating cells and response in linear scale. The system normally allows log phase growth (or near to this) for 120 h. The system has primarily been designed for large scale population genetic investigations using X-rayed stimulator cells stored frozen in the irradiated state. It suits this purpose well, and is comparable with other systems in discrimination between zero, one and two haplotype differences in families. The more refined discriminative power between low and no stimulation remains to be evaluated.

  • mlc a micro modification of the Mixed Leucocyte Culture technique
    Tissue Antigens, 2008
    Co-Authors: F. Jørgensen, L. U. Lamm
    Abstract:

    A micro-modification of the MLC technique is described, using 5 × 104 responding cells per single Culture in round bottomed microtiter plates. Over a wide range, there is a linear relation between the number of cells grown per well and the obtained response; and with the number of responding cells chosen, there is a linearity between log dose stimulating cells and response in linear scale. The system normally allows log phase growth (or near to this) for 120 h. The system has primarily been designed for large scale population genetic investigations using X-rayed stimulator cells stored frozen in the irradiated state. It suits this purpose well, and is comparable with other systems in discrimination between zero, one and two haplotype differences in families. The more refined discriminative power between low and no stimulation remains to be evaluated.

K. Matsuki - One of the best experts on this subject based on the ideXlab platform.

  • Interaction of IL‐1β, IL‐6 and tumour necrosis factor‐alpha (TNF‐α) in human T cells activated by murine antigens
    Clinical and experimental immunology, 1993
    Co-Authors: S. Panzer, M. Madden, K. Matsuki
    Abstract:

    We used a Mixed Leucocyte Culture between human T cells and irradiated murine splenocytes which allowed us to distinguish between cytokine production from the responder and stimulator cells by the use of species-specific assays for mRNA up-regulation. Using this model of T cell activation by antigen, we studied the effects of human antigen-presenting cell-derived cytokines IL-1 beta, IL-6 and TNF-alpha on the activation of human T cell subsets. We show in this system that exogenously added IL-1 beta, IL-6 and TNF-alpha induces IL-2 receptor (R) up-regulation and IL-2 production, and proliferation by both CD4+ and CD8+ cells. The addition of IL-1 beta induces IL-6 mRNA, and anti-IL-1 antibodies or an IL-1R antagonist protein completely suppresses IL-6 and TNF-alpha supported proliferation. Similarly, addition of IL-6 or TNF-alpha induces up-regulation of IL-1 beta mRNA. However, anti-IL-6 and anti-IL-6R antibodies only partially block proliferation supported by IL-1 beta. These findings suggest that IL-6 and TNF-alpha will induce IL-2R up-regulation/IL-2 secretion via the induction of IL-1 beta production.

Per A Peterson - One of the best experts on this subject based on the ideXlab platform.

  • β2 microglobulin on the cell surface relationship to hl a antigens and the Mixed Leucocyte Culture reaction
    Tissue Antigens, 2008
    Co-Authors: Bertil J Lindblom, Lars Ostberg, Per A Peterson
    Abstract:

    Fab'-fragments derived from antibodies against β2-microglobulin will completely abolish the cytotoxic effect of antibodies against HL-A serological determinants. This is the result of the Fab'-fragments competing with the antibodies against the HL-A antigens for binding to the lymphocyte surface. Reciprocally, it is shown that Fab'-fragments against the HL-A alloantigenic structures impeded the binding of Fab'-fragments to cell surface bound β2-microglobulin. Fab'-fragments against β2-microglobulin inhibit considerably the Mixed Leucocyte Culture reaction (MLR). The main effect is on the responding cells, whereas when the stimulating cells are treated with Fab'-fragments against β2-microglobulin they do not (or, at most, only slightly) change their characteristics in the MLR. Fab'-fragments against immunoglobulin light chains do not influence the MLR significantly, whereas Fab'-fragments against HL-A antigens depress the response. Fab'-fragments against β2-microglobulin do not interfere with the mitogenic stimulation of PHA, whereas Fab'-fragments from the antiserum used in this study against HL-A8 reduced the mitogenic effect of PHA considerably.

Y. Lin - One of the best experts on this subject based on the ideXlab platform.

  • Renal allograft rejection: expression and function of VCAM‐1 on tubular epithelial cells
    Clinical and experimental immunology, 2008
    Co-Authors: Y. Lin, John A. Kirby, Da Browell, A. R. Morley, Brian K. Shenton, George Proud, R. M. R. Taylor
    Abstract:

    The interaction between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) is known to play an important role in stabilizing the adhesion of lymphocytes to endothelial cells. Such cellular adhesion is crucial to many immunological processes including lymphocyte-mediated cell lysis. In this study the expression of VCAM-1 on renal tubular epithelial cells is demonstrated on biopsy sections recovered during acute renal allograft rejection. Experiments performed using epithelial cells Cultured from renal tubules show that VCAM-1 is up-regulated by addition of the inflammatory cytokines tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). Combination of TNF-alpha and IFN-gamma synergized to induce high levels of VCAM-1 expression. Further experiments demonstrated that the cytokines produced by activated lymphocytes in Mixed Leucocyte Culture also up-regulate expression of VCAM-1. Assays of the adhesion of lymphoid cells to Cultured renal epithelial cells showed that cytokine pretreatment of the renal cells enhanced the binding of lymphoid cells. The proportion of bound lymphoid cells was significantly reduced by addition of an antibody capable of blocking the interaction of VCAM-1 with VLA-4. This result indicated that the VCAM-1 induced on renal epithelial cells by inflammatory cytokines is functionally capable of binding VLA-4, thereby enhancing the adhesion of potentially graft-damaging lymphoid cells.