The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Xu Ya Yu - One of the best experts on this subject based on the ideXlab platform.
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strigolactone mediates jasmonic acid induced lipid production in microalga Monoraphidium sp qly 1 under nitrogen deficiency conditions
Bioresource Technology, 2020Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract The roles of jasmonic acid (JA) in the regulation of cell growth and lipid biosynthesis under the combination of strigolactone (SL) treatment and nitrogen deficiency (ND) were investigated. In this work, the optimised ND condition (46.18%) and ND combined with SL treatment (53.71%) showed 1.11- and 1.29-fold increases in lipid content in Monoraphidium sp. QLY-1 compared with the control condition (41.57%). The levels of JA, glutathione (GSH), and γ-aminobutyric acid (GABA) and lipogenic genes expression were upregulated by the combination of SL and ND, but the ROS level was decreased. Furthermore, exogenous JA supplementation induced the highest lipid content (57.12%) and productivity (312.35 mg L−1 d−1) under ND combined with SL treatment. This study provided a combined strategy for enhancing lipid production and supplied novel insights into the role of JA signalling in regulating lipid synthesis and oxidative stress in microalgae by combining SL treatment with ND.
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improvement in lipid production in Monoraphidium sp qly 1 by combining fulvic acid treatment and salinity stress
Bioresource Technology, 2019Co-Authors: Ximing Li, Yong Teng Zhao, Peng Zhao, Tao Li, Xingyu Li, Xu Ya YuAbstract:Abstract The effects of the combined treatment of fulvic acid (FA) and salinity stress on lipid production in Monoraphidium sp. QLY-1 at multiple levels was investigated in this study. The results indicated that the highest lipid content (59.53%) in QLY-1 was achieved by combining FA treatment and salinity stress. Compared with the control group and FA addition alone, the group treated with both FA and salinity stress had increased contents of reactive oxygen species (ROS), antioxidases, and nitric oxide (NO). Additionally, the addition of FA enhanced the expression levels of mitogen-activated protein kinases (MAPKs) and key genes related to lipid biosynthesis in QLY-1 under salinity stress. Collectively, biochemical analyses indicated that ROS, NO, MAPK, expression of lipid biosynthesis-related genes and antioxidant systems were involved in the lipid biosynthesis pathways of QLY-1 under the combined treatment of FA and salinity stress.
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enhancement of lipid accumulation in Monoraphidium sp qly 1 by induction of strigolactone
Bioresource Technology, 2019Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract In this study, the effects of strigolactone (SL) on the biomass, lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The lipid content and lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca2+ and lipid biosynthesis gene transcription. Subsequently, the relationship between Ca2+ and nitric oxide (NO) in the regulation of cell growth and lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca2+ regulated cell growth and lipid biosynthesis by modulating the levels of NO and lipid biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca2+ and NO signalling in the manipulation of lipid biosynthesis in microalgae under SL treatment.
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enhancing biomass lipid production and nutrient utilization of the microalga Monoraphidium sp qlz 3 in walnut shell extracts supplemented with carbon dioxide
Bioresource Technology, 2019Co-Authors: Xunzan Dong, Yong Teng Zhao, Wei Ding, Xu Ya YuAbstract:Abstract Microalgae are a promising biofuel resource, but their high cost and low productivity hinder their commercial applications. In the present study, Monoraphidium sp. QLZ-3 was cultivated in walnut shell extracts (WSE) supplemented with carbon dioxide (CO2). Biomass was enhanced from 0.40 g L−1 to 1.18 g L−1, and lipid content reached 49.54% in WSE-12% CO2 media. Biomass and lipid productivity reached 196.88 and 97.52 mg L−1 d−1, which were 1.33- and 1.57-fold higher than those of the control, respectively. The amount of carbohydrates increased, but the protein contents decreased. Furthermore, the application of CO2 promoted nutrient and polyphenol absorption and upregulated the expression levels of lipid biosynthetic genes of this WSE-cultivated alga. These results indicated that coupling WSE and CO2 could be an efficient strategy to enhance biofuel production by microalgae.
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enhancement of lipid production and nutrient removal of Monoraphidium sp fxy 10 by combined melatonin and molasses wastewater treatment
Journal of The Taiwan Institute of Chemical Engineers, 2019Co-Authors: Xunzan Dong, Yong Teng Zhao, Peng Zhao, Tao Li, Li Huang, Junwei Xu, Xu Ya YuAbstract:Abstract Molasses wastewater can be utilized for microalgae cultivation, and the harvested algal biomass is suitable for biofuel production. In this study, the effects of melatonin on the metabolism and activities of three key lipid biosynthetic enzymes of Monoraphidium sp. FXY-10 cultured in molasses wastewater were evaluated. The maximal biomass (1.21 g L−1) and lipid content (68.69%) were obtained during melatonin treatment and were approximately 1.42- and 1.15-fold, respectively, of the group not treated with melatonin. The carbohydrate and protein contents of melatonin-treated cells were also increased. MT treatment upregulated the activities of malic enzyme and acetyl-CoA carboxylase but downregulated phosphoenolpyruvate carboxylase activity, which was correlated with lipid accumulation in FXY-10. MT-treated samples showed greater potentials for removing organic carbon (92.33% chemical oxygen demand removal) and nutrients (90.07% nitrogen removal, 86.04% phosphorus removal) than the non-treated samples. Additionally, the economic feasibility of melatonin treatment demonstrated that a combined strategy is ideal for molasses wastewater treatment and biofuel production.
Peng Zhao - One of the best experts on this subject based on the ideXlab platform.
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strigolactone mediates jasmonic acid induced lipid production in microalga Monoraphidium sp qly 1 under nitrogen deficiency conditions
Bioresource Technology, 2020Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract The roles of jasmonic acid (JA) in the regulation of cell growth and lipid biosynthesis under the combination of strigolactone (SL) treatment and nitrogen deficiency (ND) were investigated. In this work, the optimised ND condition (46.18%) and ND combined with SL treatment (53.71%) showed 1.11- and 1.29-fold increases in lipid content in Monoraphidium sp. QLY-1 compared with the control condition (41.57%). The levels of JA, glutathione (GSH), and γ-aminobutyric acid (GABA) and lipogenic genes expression were upregulated by the combination of SL and ND, but the ROS level was decreased. Furthermore, exogenous JA supplementation induced the highest lipid content (57.12%) and productivity (312.35 mg L−1 d−1) under ND combined with SL treatment. This study provided a combined strategy for enhancing lipid production and supplied novel insights into the role of JA signalling in regulating lipid synthesis and oxidative stress in microalgae by combining SL treatment with ND.
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improvement in lipid production in Monoraphidium sp qly 1 by combining fulvic acid treatment and salinity stress
Bioresource Technology, 2019Co-Authors: Ximing Li, Yong Teng Zhao, Peng Zhao, Tao Li, Xingyu Li, Xu Ya YuAbstract:Abstract The effects of the combined treatment of fulvic acid (FA) and salinity stress on lipid production in Monoraphidium sp. QLY-1 at multiple levels was investigated in this study. The results indicated that the highest lipid content (59.53%) in QLY-1 was achieved by combining FA treatment and salinity stress. Compared with the control group and FA addition alone, the group treated with both FA and salinity stress had increased contents of reactive oxygen species (ROS), antioxidases, and nitric oxide (NO). Additionally, the addition of FA enhanced the expression levels of mitogen-activated protein kinases (MAPKs) and key genes related to lipid biosynthesis in QLY-1 under salinity stress. Collectively, biochemical analyses indicated that ROS, NO, MAPK, expression of lipid biosynthesis-related genes and antioxidant systems were involved in the lipid biosynthesis pathways of QLY-1 under the combined treatment of FA and salinity stress.
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enhancement of lipid accumulation in Monoraphidium sp qly 1 by induction of strigolactone
Bioresource Technology, 2019Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract In this study, the effects of strigolactone (SL) on the biomass, lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The lipid content and lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca2+ and lipid biosynthesis gene transcription. Subsequently, the relationship between Ca2+ and nitric oxide (NO) in the regulation of cell growth and lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca2+ regulated cell growth and lipid biosynthesis by modulating the levels of NO and lipid biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca2+ and NO signalling in the manipulation of lipid biosynthesis in microalgae under SL treatment.
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enhancement of lipid production and nutrient removal of Monoraphidium sp fxy 10 by combined melatonin and molasses wastewater treatment
Journal of The Taiwan Institute of Chemical Engineers, 2019Co-Authors: Xunzan Dong, Yong Teng Zhao, Peng Zhao, Tao Li, Li Huang, Junwei Xu, Xu Ya YuAbstract:Abstract Molasses wastewater can be utilized for microalgae cultivation, and the harvested algal biomass is suitable for biofuel production. In this study, the effects of melatonin on the metabolism and activities of three key lipid biosynthetic enzymes of Monoraphidium sp. FXY-10 cultured in molasses wastewater were evaluated. The maximal biomass (1.21 g L−1) and lipid content (68.69%) were obtained during melatonin treatment and were approximately 1.42- and 1.15-fold, respectively, of the group not treated with melatonin. The carbohydrate and protein contents of melatonin-treated cells were also increased. MT treatment upregulated the activities of malic enzyme and acetyl-CoA carboxylase but downregulated phosphoenolpyruvate carboxylase activity, which was correlated with lipid accumulation in FXY-10. MT-treated samples showed greater potentials for removing organic carbon (92.33% chemical oxygen demand removal) and nutrients (90.07% nitrogen removal, 86.04% phosphorus removal) than the non-treated samples. Additionally, the economic feasibility of melatonin treatment demonstrated that a combined strategy is ideal for molasses wastewater treatment and biofuel production.
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melatonin enhances lipid production in Monoraphidium sp qly 1 under nitrogen deficiency conditions via a multi level mechanism
Bioresource Technology, 2018Co-Authors: Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Dafei Li, Xu Ya YuAbstract:Abstract In this study, melatonin (MT) promoted lipid accumulation in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions. The lipid accumulation increased 1.22- and 1.36-fold compared with a nitrogen-starved medium and a normal BG-11 medium, respectively. The maximum lipid content was 51.38%. The reactive oxygen species (ROS) level in the presence of melatonin was lower than that in the control group, likely because of the high antioxidant activities. The application of melatonin upregulated the gibberellin acid (GA) production and rbcL and accD expression levels but downregulated the abscisic acid (ABA) content and pepc expression levels. These findings demonstrated that exogenous melatonin could further improve the lipid production in Monoraphidium sp. QLY-1 by regulating antioxidant systems, signalling molecules, and lipid biosynthesis-related gene expression under nitrogen deficiency conditions.
Tao Li - One of the best experts on this subject based on the ideXlab platform.
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strigolactone mediates jasmonic acid induced lipid production in microalga Monoraphidium sp qly 1 under nitrogen deficiency conditions
Bioresource Technology, 2020Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract The roles of jasmonic acid (JA) in the regulation of cell growth and lipid biosynthesis under the combination of strigolactone (SL) treatment and nitrogen deficiency (ND) were investigated. In this work, the optimised ND condition (46.18%) and ND combined with SL treatment (53.71%) showed 1.11- and 1.29-fold increases in lipid content in Monoraphidium sp. QLY-1 compared with the control condition (41.57%). The levels of JA, glutathione (GSH), and γ-aminobutyric acid (GABA) and lipogenic genes expression were upregulated by the combination of SL and ND, but the ROS level was decreased. Furthermore, exogenous JA supplementation induced the highest lipid content (57.12%) and productivity (312.35 mg L−1 d−1) under ND combined with SL treatment. This study provided a combined strategy for enhancing lipid production and supplied novel insights into the role of JA signalling in regulating lipid synthesis and oxidative stress in microalgae by combining SL treatment with ND.
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improvement in lipid production in Monoraphidium sp qly 1 by combining fulvic acid treatment and salinity stress
Bioresource Technology, 2019Co-Authors: Ximing Li, Yong Teng Zhao, Peng Zhao, Tao Li, Xingyu Li, Xu Ya YuAbstract:Abstract The effects of the combined treatment of fulvic acid (FA) and salinity stress on lipid production in Monoraphidium sp. QLY-1 at multiple levels was investigated in this study. The results indicated that the highest lipid content (59.53%) in QLY-1 was achieved by combining FA treatment and salinity stress. Compared with the control group and FA addition alone, the group treated with both FA and salinity stress had increased contents of reactive oxygen species (ROS), antioxidases, and nitric oxide (NO). Additionally, the addition of FA enhanced the expression levels of mitogen-activated protein kinases (MAPKs) and key genes related to lipid biosynthesis in QLY-1 under salinity stress. Collectively, biochemical analyses indicated that ROS, NO, MAPK, expression of lipid biosynthesis-related genes and antioxidant systems were involved in the lipid biosynthesis pathways of QLY-1 under the combined treatment of FA and salinity stress.
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enhancement of lipid accumulation in Monoraphidium sp qly 1 by induction of strigolactone
Bioresource Technology, 2019Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract In this study, the effects of strigolactone (SL) on the biomass, lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The lipid content and lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca2+ and lipid biosynthesis gene transcription. Subsequently, the relationship between Ca2+ and nitric oxide (NO) in the regulation of cell growth and lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca2+ regulated cell growth and lipid biosynthesis by modulating the levels of NO and lipid biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca2+ and NO signalling in the manipulation of lipid biosynthesis in microalgae under SL treatment.
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enhancement of lipid production and nutrient removal of Monoraphidium sp fxy 10 by combined melatonin and molasses wastewater treatment
Journal of The Taiwan Institute of Chemical Engineers, 2019Co-Authors: Xunzan Dong, Yong Teng Zhao, Peng Zhao, Tao Li, Li Huang, Junwei Xu, Xu Ya YuAbstract:Abstract Molasses wastewater can be utilized for microalgae cultivation, and the harvested algal biomass is suitable for biofuel production. In this study, the effects of melatonin on the metabolism and activities of three key lipid biosynthetic enzymes of Monoraphidium sp. FXY-10 cultured in molasses wastewater were evaluated. The maximal biomass (1.21 g L−1) and lipid content (68.69%) were obtained during melatonin treatment and were approximately 1.42- and 1.15-fold, respectively, of the group not treated with melatonin. The carbohydrate and protein contents of melatonin-treated cells were also increased. MT treatment upregulated the activities of malic enzyme and acetyl-CoA carboxylase but downregulated phosphoenolpyruvate carboxylase activity, which was correlated with lipid accumulation in FXY-10. MT-treated samples showed greater potentials for removing organic carbon (92.33% chemical oxygen demand removal) and nutrients (90.07% nitrogen removal, 86.04% phosphorus removal) than the non-treated samples. Additionally, the economic feasibility of melatonin treatment demonstrated that a combined strategy is ideal for molasses wastewater treatment and biofuel production.
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melatonin enhances lipid production in Monoraphidium sp qly 1 under nitrogen deficiency conditions via a multi level mechanism
Bioresource Technology, 2018Co-Authors: Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Dafei Li, Xu Ya YuAbstract:Abstract In this study, melatonin (MT) promoted lipid accumulation in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions. The lipid accumulation increased 1.22- and 1.36-fold compared with a nitrogen-starved medium and a normal BG-11 medium, respectively. The maximum lipid content was 51.38%. The reactive oxygen species (ROS) level in the presence of melatonin was lower than that in the control group, likely because of the high antioxidant activities. The application of melatonin upregulated the gibberellin acid (GA) production and rbcL and accD expression levels but downregulated the abscisic acid (ABA) content and pepc expression levels. These findings demonstrated that exogenous melatonin could further improve the lipid production in Monoraphidium sp. QLY-1 by regulating antioxidant systems, signalling molecules, and lipid biosynthesis-related gene expression under nitrogen deficiency conditions.
Yong Teng Zhao - One of the best experts on this subject based on the ideXlab platform.
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strigolactone mediates jasmonic acid induced lipid production in microalga Monoraphidium sp qly 1 under nitrogen deficiency conditions
Bioresource Technology, 2020Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract The roles of jasmonic acid (JA) in the regulation of cell growth and lipid biosynthesis under the combination of strigolactone (SL) treatment and nitrogen deficiency (ND) were investigated. In this work, the optimised ND condition (46.18%) and ND combined with SL treatment (53.71%) showed 1.11- and 1.29-fold increases in lipid content in Monoraphidium sp. QLY-1 compared with the control condition (41.57%). The levels of JA, glutathione (GSH), and γ-aminobutyric acid (GABA) and lipogenic genes expression were upregulated by the combination of SL and ND, but the ROS level was decreased. Furthermore, exogenous JA supplementation induced the highest lipid content (57.12%) and productivity (312.35 mg L−1 d−1) under ND combined with SL treatment. This study provided a combined strategy for enhancing lipid production and supplied novel insights into the role of JA signalling in regulating lipid synthesis and oxidative stress in microalgae by combining SL treatment with ND.
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improvement in lipid production in Monoraphidium sp qly 1 by combining fulvic acid treatment and salinity stress
Bioresource Technology, 2019Co-Authors: Ximing Li, Yong Teng Zhao, Peng Zhao, Tao Li, Xingyu Li, Xu Ya YuAbstract:Abstract The effects of the combined treatment of fulvic acid (FA) and salinity stress on lipid production in Monoraphidium sp. QLY-1 at multiple levels was investigated in this study. The results indicated that the highest lipid content (59.53%) in QLY-1 was achieved by combining FA treatment and salinity stress. Compared with the control group and FA addition alone, the group treated with both FA and salinity stress had increased contents of reactive oxygen species (ROS), antioxidases, and nitric oxide (NO). Additionally, the addition of FA enhanced the expression levels of mitogen-activated protein kinases (MAPKs) and key genes related to lipid biosynthesis in QLY-1 under salinity stress. Collectively, biochemical analyses indicated that ROS, NO, MAPK, expression of lipid biosynthesis-related genes and antioxidant systems were involved in the lipid biosynthesis pathways of QLY-1 under the combined treatment of FA and salinity stress.
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enhancing biomass lipid production and nutrient utilization of the microalga Monoraphidium sp qlz 3 in walnut shell extracts supplemented with carbon dioxide
Bioresource Technology, 2019Co-Authors: Xunzan Dong, Yong Teng Zhao, Wei Ding, Xu Ya YuAbstract:Abstract Microalgae are a promising biofuel resource, but their high cost and low productivity hinder their commercial applications. In the present study, Monoraphidium sp. QLZ-3 was cultivated in walnut shell extracts (WSE) supplemented with carbon dioxide (CO2). Biomass was enhanced from 0.40 g L−1 to 1.18 g L−1, and lipid content reached 49.54% in WSE-12% CO2 media. Biomass and lipid productivity reached 196.88 and 97.52 mg L−1 d−1, which were 1.33- and 1.57-fold higher than those of the control, respectively. The amount of carbohydrates increased, but the protein contents decreased. Furthermore, the application of CO2 promoted nutrient and polyphenol absorption and upregulated the expression levels of lipid biosynthetic genes of this WSE-cultivated alga. These results indicated that coupling WSE and CO2 could be an efficient strategy to enhance biofuel production by microalgae.
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enhancement of lipid accumulation in Monoraphidium sp qly 1 by induction of strigolactone
Bioresource Technology, 2019Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract In this study, the effects of strigolactone (SL) on the biomass, lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The lipid content and lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca2+ and lipid biosynthesis gene transcription. Subsequently, the relationship between Ca2+ and nitric oxide (NO) in the regulation of cell growth and lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca2+ regulated cell growth and lipid biosynthesis by modulating the levels of NO and lipid biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca2+ and NO signalling in the manipulation of lipid biosynthesis in microalgae under SL treatment.
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enhancement of lipid production and nutrient removal of Monoraphidium sp fxy 10 by combined melatonin and molasses wastewater treatment
Journal of The Taiwan Institute of Chemical Engineers, 2019Co-Authors: Xunzan Dong, Yong Teng Zhao, Peng Zhao, Tao Li, Li Huang, Junwei Xu, Xu Ya YuAbstract:Abstract Molasses wastewater can be utilized for microalgae cultivation, and the harvested algal biomass is suitable for biofuel production. In this study, the effects of melatonin on the metabolism and activities of three key lipid biosynthetic enzymes of Monoraphidium sp. FXY-10 cultured in molasses wastewater were evaluated. The maximal biomass (1.21 g L−1) and lipid content (68.69%) were obtained during melatonin treatment and were approximately 1.42- and 1.15-fold, respectively, of the group not treated with melatonin. The carbohydrate and protein contents of melatonin-treated cells were also increased. MT treatment upregulated the activities of malic enzyme and acetyl-CoA carboxylase but downregulated phosphoenolpyruvate carboxylase activity, which was correlated with lipid accumulation in FXY-10. MT-treated samples showed greater potentials for removing organic carbon (92.33% chemical oxygen demand removal) and nutrients (90.07% nitrogen removal, 86.04% phosphorus removal) than the non-treated samples. Additionally, the economic feasibility of melatonin treatment demonstrated that a combined strategy is ideal for molasses wastewater treatment and biofuel production.
Junwei Xu - One of the best experts on this subject based on the ideXlab platform.
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strigolactone mediates jasmonic acid induced lipid production in microalga Monoraphidium sp qly 1 under nitrogen deficiency conditions
Bioresource Technology, 2020Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract The roles of jasmonic acid (JA) in the regulation of cell growth and lipid biosynthesis under the combination of strigolactone (SL) treatment and nitrogen deficiency (ND) were investigated. In this work, the optimised ND condition (46.18%) and ND combined with SL treatment (53.71%) showed 1.11- and 1.29-fold increases in lipid content in Monoraphidium sp. QLY-1 compared with the control condition (41.57%). The levels of JA, glutathione (GSH), and γ-aminobutyric acid (GABA) and lipogenic genes expression were upregulated by the combination of SL and ND, but the ROS level was decreased. Furthermore, exogenous JA supplementation induced the highest lipid content (57.12%) and productivity (312.35 mg L−1 d−1) under ND combined with SL treatment. This study provided a combined strategy for enhancing lipid production and supplied novel insights into the role of JA signalling in regulating lipid synthesis and oxidative stress in microalgae by combining SL treatment with ND.
-
enhancement of lipid accumulation in Monoraphidium sp qly 1 by induction of strigolactone
Bioresource Technology, 2019Co-Authors: Xueting Song, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Xu Ya YuAbstract:Abstract In this study, the effects of strigolactone (SL) on the biomass, lipid content, biochemical properties, and gene transcription of Monoraphidium sp. QLY-1 were examined. The lipid content and lipid productivity increased by 61% and 55% in QLY-1 under 1 μM SL induction compared to the control group, respectively. SL also upregulated the levels of endogenous NO and Ca2+ and lipid biosynthesis gene transcription. Subsequently, the relationship between Ca2+ and nitric oxide (NO) in the regulation of cell growth and lipid accumulation of QLY-1 under SL induction conditions was analysed. An increase in endogenous Ca2+ regulated cell growth and lipid biosynthesis by modulating the levels of NO and lipid biosynthesis-related gene expression. Collectively, this study provided a valuable approach for biofuel production from microalgae under SL induction and demonstrated that there is crucial crosstalk between the Ca2+ and NO signalling in the manipulation of lipid biosynthesis in microalgae under SL treatment.
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enhancement of lipid production and nutrient removal of Monoraphidium sp fxy 10 by combined melatonin and molasses wastewater treatment
Journal of The Taiwan Institute of Chemical Engineers, 2019Co-Authors: Xunzan Dong, Yong Teng Zhao, Peng Zhao, Tao Li, Li Huang, Junwei Xu, Xu Ya YuAbstract:Abstract Molasses wastewater can be utilized for microalgae cultivation, and the harvested algal biomass is suitable for biofuel production. In this study, the effects of melatonin on the metabolism and activities of three key lipid biosynthetic enzymes of Monoraphidium sp. FXY-10 cultured in molasses wastewater were evaluated. The maximal biomass (1.21 g L−1) and lipid content (68.69%) were obtained during melatonin treatment and were approximately 1.42- and 1.15-fold, respectively, of the group not treated with melatonin. The carbohydrate and protein contents of melatonin-treated cells were also increased. MT treatment upregulated the activities of malic enzyme and acetyl-CoA carboxylase but downregulated phosphoenolpyruvate carboxylase activity, which was correlated with lipid accumulation in FXY-10. MT-treated samples showed greater potentials for removing organic carbon (92.33% chemical oxygen demand removal) and nutrients (90.07% nitrogen removal, 86.04% phosphorus removal) than the non-treated samples. Additionally, the economic feasibility of melatonin treatment demonstrated that a combined strategy is ideal for molasses wastewater treatment and biofuel production.
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melatonin enhances lipid production in Monoraphidium sp qly 1 under nitrogen deficiency conditions via a multi level mechanism
Bioresource Technology, 2018Co-Authors: Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Dafei Li, Xu Ya YuAbstract:Abstract In this study, melatonin (MT) promoted lipid accumulation in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions. The lipid accumulation increased 1.22- and 1.36-fold compared with a nitrogen-starved medium and a normal BG-11 medium, respectively. The maximum lipid content was 51.38%. The reactive oxygen species (ROS) level in the presence of melatonin was lower than that in the control group, likely because of the high antioxidant activities. The application of melatonin upregulated the gibberellin acid (GA) production and rbcL and accD expression levels but downregulated the abscisic acid (ABA) content and pepc expression levels. These findings demonstrated that exogenous melatonin could further improve the lipid production in Monoraphidium sp. QLY-1 by regulating antioxidant systems, signalling molecules, and lipid biosynthesis-related gene expression under nitrogen deficiency conditions.
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a strategy for promoting lipid production in green microalgae Monoraphidium sp qly 1 by combined melatonin and photoinduction
Bioresource Technology, 2017Co-Authors: Dafei Li, Yong Teng Zhao, Peng Zhao, Tao Li, Junwei Xu, Wei Ding, Xu Ya YuAbstract:Abstract Microalgae lipids are potential candidates for the production of renewable biodiesel. The combination of plant hormones and two-step cultivation regulates lipid production in microalgae. A strategy for promoting lipid accumulation in Monoraphidium sp. QLY-1 by combining exogenous melatonin (MT) and photoinduction was developed. The effects of melatonin on the lipid content, reactive oxygen species (ROS), and activities of three key fatty acid biosynthetic enzyme in Monoraphidium sp. QLY-1 were investigated. The lipid content increased by 1.32-fold under 1 μM melatonin treatment. The maximum lipid content achieved was 49.6%. However, the protein and carbohydrate contents decreased rapidly from 57.21% to 47.96% and from 53.4% to 37.71%, respectively. Biochemical and physiological analyses suggested that the ROS and lipid biosynthesis-related enzyme activities correlated with increased lipid accumulation under photo-melatonin induction conditions.