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Maria Ligia Rodrigues Macedo - One of the best experts on this subject based on the ideXlab platform.
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insecticidal action of annona coriacea lectin against the flour Moth anagasta kuehniella and the rice Moth corcyra cephalonica lepidoptera pyralidae
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2007Co-Authors: Mirela B Coelho, Sergio Marangoni, Maria Ligia Rodrigues MacedoAbstract:Abstract Annona coriacea lectin (ACLEC) was tested for insecticidal activity against larvae of two pyralid Moths, Anagasta kuehniella and Corcyra cephalonica. ACLEC produced ∼ 50% mortality and mass loss in A. kuehniella larvae when incorporated into an artificial diet at levels of 1.5% and 1.0% (w/w), respectively. In contrast, the inclusion of up to 2% ACLEC in the diet did not significantly decrease the survival or weight of C. cephalonica larvae. The nutritional indices for A. kuehniella and C. cephalonica suggested that ACLEC had a multi-mechanistic mode of action and was an antifeedant for both insects. The toxicity in A. kuehniella apparently resulted from a change in the gut membrane environment and consequent disruption of digestive enzyme recycling mechanisms. Affinity chromatography showed that ACLEC bound to midgut proteins of A. kuehniella and C. cephalonica. However, the 14 kDa subunit of ACLEC was not digested by midgut proteases of A. kuehniella, but was degraded by the corresponding C. cephalonica proteases within a few hours. These findings suggest the possibility of using ACLEC to engineer crop plants.
Shujun Wei - One of the best experts on this subject based on the ideXlab platform.
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chromosome level genome of the peach fruit Moth carposina sasakii lepidoptera carposinidae provides a resource for evolutionary studies on Moths
Molecular Ecology Resources, 2021Co-Authors: Lijun Cao, Wei Song, Lei Yue, Shaokun Guo, Jincui Chen, Yajun Gong, Ary A Hoffmann, Shujun WeiAbstract:The peach fruit Moth (PFM), Carposina sasakii Matsumura, is a major phytophagous orchard pest widely distributed across Northeast Asia. Here, we report the chromosome-level genome for the PFM, representing the first genome for the family Carposinidae, from the lepidopteran superfamily Copromorphoidea. The genome was assembled into 404.83 Mb sequences using PacBio long-read and Illumina short-read sequences, including 275 contigs, with a contig N50 length of 2.62 Mb. All contigs were assembled into 31 linkage groups assisted by the Hi-C technique, including 30 autosomes and a Z chromosome. BUSCO analysis showed that 98.3% of genes were complete and 0.4% of genes were fragmented, while 1.3% of genes were missing in the assembled genome. In total, 21,697 protein-coding genes were predicted, of which 84.80% were functionally annotated. Because of the importance of diapause triggered by photoperiod in PFM, five circadian genes in the PFM as well as in the other related species were annotated, and potential genes related to diapause and photoperiodic reaction were also identified from transcriptome sequencing. In addition, manual annotation of detoxification gene families was undertaken and showed a higher number of glutathione S-transferase (GST) gene in PFM than in most other lepidopterans, in contrast to a lower number of uridine diphosphate (UDP)-glycosyltransferase (UGT) gene, carboxyl/cholinesterases (CCE) gene and cytochrome P450 monooxygenase (P450) gene, suggesting different detoxication pathways in this Moth. The high-quality genome provides a resource for comparative evolutionary studies of this Moth and its relatives within the context of radiations across Lepidoptera.
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chromosome level genome of the peach fruit Moth carposina sasakii lepidoptera carposinidae provides a resource for evolutionary studies on Moths
Authorea Preprints, 2020Co-Authors: Lijun Cao, Wei Song, Lei Yue, Shaokun Guo, Jincui Chen, Yajun Gong, Ary A Hoffmann, Shujun WeiAbstract:The peach fruit Moth (PFM), Carposina sasakii Matsumura, is a major phytophagous orchard pest widely distributed across Northeast Asia. Here, we report the chromosome-level genome for the PFM, representing the first genome for the family Carposinidae, from the lepidopteran superfamily Copromorphoidea. The genome was assembled into 404.83 Mb sequences using PacBio long-read and Illumina short-read sequences, including 275 contigs, with a contig N50 length of 2.62 Mb. All contigs were assembled into 32 linkage groups assisted by the Hi-C technique, including 30 autosomes, a female specific W chromosome and a Z chromosome. BUSCO analysis showed that 98.2% genes were complete and 0.4% of genes were fragmented, while 1.4% of genes were missing in the assembled genome. In total, 23,218 protein-coding genes were predicted, of which 82.72% were functionally annotated. Because of the importance of diapause triggered by photoperiod in PFM, five circadian genes in the PFM as well as in the other related species were annotated, and potential genes related to diapause and photoperiodic reaction were also identified from transcriptome sequencing. In addition, manual annotation of detoxification gene families was undertaken and showed a higher number of ABC and GST genes in PFM than in most other lepidopterans, in contrast to a lower number of UGT genes, suggesting different detoxication pathways in this Moth. The high-quality genome provides a resource for comparative evolutionary studies of this Moth and its relatives within the context of radiations across Lepidoptera.
Vladimir L Katanaev - One of the best experts on this subject based on the ideXlab platform.
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Alternative Moth-eye nanostructures: antireflective properties and composition of dimpled corneal nanocoatings in silk-Moth ancestors
Journal of Nanobiotechnology, 2017Co-Authors: Mikhail Kryuchkov, Jannis Lehmann, Manfred Fiebig, Jakob Schaab, Vsevolod Cherepanov, Artem Blagodatski, Vladimir L KatanaevAbstract:Moth-eye nanostructures are a well-known example of biological antireflective surfaces formed by pseudoregular arrays of nipples and are often used as a template for biomimetic materials. Here, we provide morphological characterization of corneal nanostructures of Moths from the Bombycidae family, including strains of domesticated Bombyx mori silk-Moth, its wild ancestor Bombyx mandarina , and a more distantly related Apatelodes torrefacta . We find high diversification of the nanostructures and strong antireflective properties they provide. Curiously, the nano-dimple pattern of B. mandarina is found to reduce reflectance as efficiently as the nanopillars of A. torrefacta . Access to genome sequence of Bombyx further permitted us to pinpoint corneal proteins, likely contributing to formation of the antireflective nanocoatings. These findings open the door to bioengineering of nanostructures with novel properties, as well as invite industry to expand traditional Moth-eye nanocoatings with the alternative ones described here.
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MOESM1 of Alternative Moth-eye nanostructures: antireflective properties and composition of dimpled corneal nanocoatings in silk-Moth ancestors
2017Co-Authors: Mikhail Kryuchkov, Jannis Lehmann, Manfred Fiebig, Jakob Schaab, Vsevolod Cherepanov, Artem Blagodatski, Vladimir L KatanaevAbstract:Additional file 1: Figure S1. Fourier analysis of the Bombyx corneal nanostructures. a–f, Example of structures with increasing degree of disordering (artificial sets for panels a to c: panel size 1.3 µm, height 30 nm) and their Fourier analysis (d–f). g–l, AFM scans (g–i) of corneal surfaces of different Bombyx samples (g, B. mandarina, h, B. mori [Vn], i, B. mori[Jp]) and the corresponding Fourier spectra (j–l). This analysis reveals quasi-random vs. random structures in different samples. Quasi-random structures retain a stable period between any two neighboring peaks resulting in a ring of reflexes in their Fourier analysis (a, d). Alternatively, quasi-random structures can lack a stable period but fill the space uniformly, producing a large lattice covered by reflexes on the Fourier spectrum (b, e). Instead, random structures fill the space lopsidedly and despite the fact that the ratio of large and small objects and distances between them may exactly match those of the quasi-random structures, the Fourier spectrum shows a significant decrease of the lattice size (c, f). Fourier analysis of the B. mori [Jp] corneal nanocoatings shows that the size of lattice (i, l) of reflexes is just 12 μm−1, significantly smaller than in the case of those B. mori [Vn] and B. mandarina (17 μm−1, g, h, j, k). In the absence of clearly defined reflections, these two last-mentioned structures could be recognized as the quasi-random dimpled patterns, while the lattice size of Fourier spectrum of B. mori [Jp] indicates absence of any ordering
Mirela B Coelho - One of the best experts on this subject based on the ideXlab platform.
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insecticidal action of annona coriacea lectin against the flour Moth anagasta kuehniella and the rice Moth corcyra cephalonica lepidoptera pyralidae
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2007Co-Authors: Mirela B Coelho, Sergio Marangoni, Maria Ligia Rodrigues MacedoAbstract:Abstract Annona coriacea lectin (ACLEC) was tested for insecticidal activity against larvae of two pyralid Moths, Anagasta kuehniella and Corcyra cephalonica. ACLEC produced ∼ 50% mortality and mass loss in A. kuehniella larvae when incorporated into an artificial diet at levels of 1.5% and 1.0% (w/w), respectively. In contrast, the inclusion of up to 2% ACLEC in the diet did not significantly decrease the survival or weight of C. cephalonica larvae. The nutritional indices for A. kuehniella and C. cephalonica suggested that ACLEC had a multi-mechanistic mode of action and was an antifeedant for both insects. The toxicity in A. kuehniella apparently resulted from a change in the gut membrane environment and consequent disruption of digestive enzyme recycling mechanisms. Affinity chromatography showed that ACLEC bound to midgut proteins of A. kuehniella and C. cephalonica. However, the 14 kDa subunit of ACLEC was not digested by midgut proteases of A. kuehniella, but was degraded by the corresponding C. cephalonica proteases within a few hours. These findings suggest the possibility of using ACLEC to engineer crop plants.
Lijun Cao - One of the best experts on this subject based on the ideXlab platform.
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chromosome level genome of the peach fruit Moth carposina sasakii lepidoptera carposinidae provides a resource for evolutionary studies on Moths
Molecular Ecology Resources, 2021Co-Authors: Lijun Cao, Wei Song, Lei Yue, Shaokun Guo, Jincui Chen, Yajun Gong, Ary A Hoffmann, Shujun WeiAbstract:The peach fruit Moth (PFM), Carposina sasakii Matsumura, is a major phytophagous orchard pest widely distributed across Northeast Asia. Here, we report the chromosome-level genome for the PFM, representing the first genome for the family Carposinidae, from the lepidopteran superfamily Copromorphoidea. The genome was assembled into 404.83 Mb sequences using PacBio long-read and Illumina short-read sequences, including 275 contigs, with a contig N50 length of 2.62 Mb. All contigs were assembled into 31 linkage groups assisted by the Hi-C technique, including 30 autosomes and a Z chromosome. BUSCO analysis showed that 98.3% of genes were complete and 0.4% of genes were fragmented, while 1.3% of genes were missing in the assembled genome. In total, 21,697 protein-coding genes were predicted, of which 84.80% were functionally annotated. Because of the importance of diapause triggered by photoperiod in PFM, five circadian genes in the PFM as well as in the other related species were annotated, and potential genes related to diapause and photoperiodic reaction were also identified from transcriptome sequencing. In addition, manual annotation of detoxification gene families was undertaken and showed a higher number of glutathione S-transferase (GST) gene in PFM than in most other lepidopterans, in contrast to a lower number of uridine diphosphate (UDP)-glycosyltransferase (UGT) gene, carboxyl/cholinesterases (CCE) gene and cytochrome P450 monooxygenase (P450) gene, suggesting different detoxication pathways in this Moth. The high-quality genome provides a resource for comparative evolutionary studies of this Moth and its relatives within the context of radiations across Lepidoptera.
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chromosome level genome of the peach fruit Moth carposina sasakii lepidoptera carposinidae provides a resource for evolutionary studies on Moths
Authorea Preprints, 2020Co-Authors: Lijun Cao, Wei Song, Lei Yue, Shaokun Guo, Jincui Chen, Yajun Gong, Ary A Hoffmann, Shujun WeiAbstract:The peach fruit Moth (PFM), Carposina sasakii Matsumura, is a major phytophagous orchard pest widely distributed across Northeast Asia. Here, we report the chromosome-level genome for the PFM, representing the first genome for the family Carposinidae, from the lepidopteran superfamily Copromorphoidea. The genome was assembled into 404.83 Mb sequences using PacBio long-read and Illumina short-read sequences, including 275 contigs, with a contig N50 length of 2.62 Mb. All contigs were assembled into 32 linkage groups assisted by the Hi-C technique, including 30 autosomes, a female specific W chromosome and a Z chromosome. BUSCO analysis showed that 98.2% genes were complete and 0.4% of genes were fragmented, while 1.4% of genes were missing in the assembled genome. In total, 23,218 protein-coding genes were predicted, of which 82.72% were functionally annotated. Because of the importance of diapause triggered by photoperiod in PFM, five circadian genes in the PFM as well as in the other related species were annotated, and potential genes related to diapause and photoperiodic reaction were also identified from transcriptome sequencing. In addition, manual annotation of detoxification gene families was undertaken and showed a higher number of ABC and GST genes in PFM than in most other lepidopterans, in contrast to a lower number of UGT genes, suggesting different detoxication pathways in this Moth. The high-quality genome provides a resource for comparative evolutionary studies of this Moth and its relatives within the context of radiations across Lepidoptera.
