The Experts below are selected from a list of 76542 Experts worldwide ranked by ideXlab platform
Shinichi Yoshimura - One of the best experts on this subject based on the ideXlab platform.
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major histocompatibility complex mhc class ib gene duplications organization and expression patterns in Mouse Strain c57bl 6
BMC Genomics, 2008Co-Authors: Masato Ohtsuka, Hidetoshi Inoko, Jerzy K Kulski, Shinichi YoshimuraAbstract:Background The Mouse has more than 30 Major histocompatibility complex (Mhc) class Ib genes, most of which exist in the H2 region of chromosome 17 in distinct gene clusters. Although recent progress in Mhc research has revealed the unique roles of several Mhc class Ib genes in the immune and non-immune systems, the functions of many class Ib genes have still to be elucidated. To better understand the roles of class Ib molecules, we have characterized their gene duplication, organization and expression patterns within the H2 region of the Mouse Strain C57BL/6.
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major histocompatibility complex mhc class ib gene duplications organization and expression patterns in Mouse Strain c57bl 6
BMC Genomics, 2008Co-Authors: Masato Ohtsuka, Hidetoshi Inoko, Jerzy K Kulski, Shinichi YoshimuraAbstract:The Mouse has more than 30 Major histocompatibility complex (Mhc) class Ib genes, most of which exist in the H2 region of chromosome 17 in distinct gene clusters. Although recent progress in Mhc research has revealed the unique roles of several Mhc class Ib genes in the immune and non-immune systems, the functions of many class Ib genes have still to be elucidated. To better understand the roles of class Ib molecules, we have characterized their gene duplication, organization and expression patterns within the H2 region of the Mouse Strain C57BL/6. The genomic organization of the H2-Q, -T and -M regions was analyzed and 21 transcribed Mhc class Ib genes were identified within these regions. Dot-plot and phylogenetic analyses implied that the genes were generated by monogenic and/or multigenic duplicated events. To investigate the adult tissue, embryonic and placental expressions of these genes, we performed RT-PCR gene expression profiling using gene-specific primers. Both tissue-wide and tissue-specific gene expression patterns were obtained that suggest that the variations in the gene expression may depend on the genomic location of the duplicated genes as well as locus specific mechanisms. The genes located in the H2-T region at the centromeric end of the cluster were expressed more widely than those at the telomeric end, which showed tissue-restricted expression in spite of nucleotide sequence similarities among gene paralogs. Duplicated Mhc class Ib genes located in the H2-Q, -T and -M regions are differentially expressed in a variety of developing and adult tissues. Our findings form the basis for further functional validation studies of the Mhc class Ib gene expression profiles in specific tissues, such as the brain. The duplicated gene expression results in combination with the genome analysis suggest the possibility of long-range regulation of H2-T gene expression and/or important, but as yet unidentified nucleotide changes in the promoter or enhancer regions of the genes. Since the Mhc genomic region has diversified among Mouse Strains, it should be a useful model region for comparative analyses of the relationships between duplicated gene organization, evolution and the regulation of expression patterns.
Stephen I Deutsch - One of the best experts on this subject based on the ideXlab platform.
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d serine improves dimensions of the sociability deficit of the genetically inbred balb c Mouse Strain
Brain Research Bulletin, 2011Co-Authors: Luis F Jacome, Jessica A Burket, Amy L Herndon, William R Cannon, Stephen I DeutschAbstract:Abstract The Balb/c Mouse Strain shows quantitative deficits of sociability and is behaviorally-hypersensitive to MK-801 (dizocilpine), a noncompetitive NMDA receptor antagonist. D-Serine (560 mg/kg, intraperitoneally), a full agonist for the obligatory glycine co-agonist binding site on the NMDA receptor, increased the amount of time Balb/c mice spend in a compartment containing the enclosed social stimulus Mouse and the amount of time Balb/c mice spend exploring (sniffing) an inverted cup containing the enclosed social stimulus Mouse in a standard sociability apparatus. These effects of D-serine on the impaired sociability of the Balb/c Mouse Strain were not due to a “nonspecific” effect on locomotor activity; importantly, the locomotor activity of the Balb/c Mouse Strain decreases in the presence of an enclosed or freely-moving social stimulus Mouse. The data suggest that dimensions of the impaired sociability of the Balb/c Mouse Strain may be improved by targeted NMDA receptor agonist interventions.
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mk 801 a noncompetitive nmda receptor antagonist elicits circling behavior in the genetically inbred balb c Mouse Strain
Brain Research Bulletin, 2010Co-Authors: Jessica A Burket, Luis F Jacome, William R Cannon, Stephen I DeutschAbstract:The Balb/c Mouse is behaviorally hypersensitive to effects of MK-801 (dizocilpine), a noncompetitive NMDA receptor antagonist, and displays impaired sociability. In the current investigation, MK-801-elicited circling behavior in the genetically inbred Balb/c Mouse Strain that was either not or only minimally observed in similarly treated outbred Swiss-Webster mice. The ability of compounds to attenuate the intensity of MK-801-elicited circling behavior in the Balb/c Mouse Strain may serve as a preclinical screening paradigm for identifying effective NMDA receptor agonist interventions in the intact animal; ideally, these compounds would have therapeutic value in neuropsychiatric disorders associated with impaired sociability, such as schizophrenia and autism spectrum disorders (ASD).
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locomotor activity of the genetically inbred balb c Mouse Strain is suppressed by a socially salient stimulus
Brain Research Bulletin, 2010Co-Authors: Jessica A Burket, Amy L Herndon, Stephen I DeutschAbstract:The genetically inbred Balb/c Mouse Strain shows behavioral hypersensitivity to MK-801 (dizocilpine), a noncompetitive NMDA receptor antagonist. In the current study, the locomotor activity of male Balb/c mice was more severely reduced in the presence of a "stimulus" Mouse, compared with the outbred Swiss-Webster Mouse Strain; reduced locomotor activity of the Balb/c Mouse was observed when the stimulus Mouse was enclosed and when the two mice were allowed to interact freely. The Balb/c Mouse Strain with altered endogenous tone of NMDA receptor-mediated neurotransmission may lend itself to the identification of candidate compounds targeting impaired sociability, an important dimension of psychopathology in schizophrenia and autism spectrum disorders (ASDs). Reduced locomotor activity in the presence of a stimulus Mouse is a sensitive behavioral outcome measure of impaired sociability.
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genetically inbred balb c mice are more sensitive to an effect of flurazepam and more resistant to an effect of stress than a genetically outbred Mouse Strain
Epilepsy & Behavior, 2009Co-Authors: Jessica A Burket, John Mastropaolo, Richard B Rosse, Stephen I DeutschAbstract:The inbred Balb/c Mouse Strain was more sensitive than the outbred NIH Swiss Mouse to flurazepam's ability to antagonize electrically precipitated seizures. In prior work, a reduction in flurazepam's antiseizure efficacy was not observed 24h after forcing Balb/c mice to swim for up to 10 min in ambient temperature water. Thus, we wondered if a stress-induced reduction would be observed after forcing mice to swim for up to 10 min in cold (6 degrees C) water, a more severe stress. The current data show that 24 h after exposure to this stress, the ability of flurazepam to raise the threshold voltage for the elicitation of tonic hindlimb extension in the Balb/c Mouse Strain was reduced. The genetically inbred Balb/c Mouse Strain is emerging as an interesting animal model in which to study interactions of stress and genetic factors that affect endogenous neurotransmission mediated by l-glutamate and GABA at the NMDA and GABA(A) receptor complexes, respectively.
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expression of nr1 nr2a and nr2b nmda receptor subunits is not altered in the genetically inbred balb c Mouse Strain with heightened behavioral sensitivity to mk 801 a noncompetitive nmda receptor antagonist
European Neuropsychopharmacology, 2008Co-Authors: Pinyu Perera, Jack H Lichy, John Mastropaolo, Richard B Rosse, Stephen I DeutschAbstract:The genetically-inbred Balb/c Mouse Strain shows heightened sensitivity to the ability of MK-801 (dizocilpine), a noncompetitive NMDA receptor antagonist, to raise the threshold voltage necessary to precipitate tonic hindlimb extension and elicit irregular episodes of intense jumping behavior (referred to as "popping"), relative to other inbred Mouse Strains and the outbred NIH Swiss Mouse. Moreover, an allosteric modulatory effect of sarcosine, a glycine reuptake inhibitor, on MK-801's antagonism of electrically precipitated seizures was detected 24 h after Balb/c mice were forced to swim in cold water for up to 10 min; this was not observed in unstressed Balb/c mice or stressed or unstressed NIH Swiss mice. Phencyclidine (PCP), a noncompetitive NMDA receptor antagonist that binds to the same hydrophobic channel domain as MK-801, precipitates a schizophreniform psychosis in susceptible individuals that shares descriptive similarities with schizophrenia. This observation has led to the hypothesis that NMDA receptor hypofunction (NRH) is involved in the pathophysiology of schizophrenia and the testing of pharmacotherapeutic strategies to facilitate NMDA receptor-mediated neurotransmission in patients with this disorder (e.g., glycine reuptake inhibitors). The heightened behavioral sensitivity of the Balb/c Mouse to MK-801 suggests that this Mouse Strain may be a useful model to study "psychosis-proneness" and screen for positive allosteric modulators of NMDA receptor-mediated neurotransmission. Conceivably, Strain differences in the pharmacology of the NMDA receptor are due to differences in the relative expression of individual NMDA receptor subunits to each other (i.e., combinatorial regulation). The current study compared the normal protein expression patterns of six of the eight identified splice variant isoforms of the NR1 NMDA receptor subunit, and NR2A and NR2B subunits in the hippocampus and cerebral cortex of Balb/c and NIH Swiss mice. The heightened behavioral sensitivity of the Balb/c genetically-inbred Mouse Strain to MK-801, compared to the outbred NIH Swiss Mouse Strain, does not appear to result from relative alterations of expression of these NMDA receptor protein subunits that were examined.
Masato Ohtsuka - One of the best experts on this subject based on the ideXlab platform.
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major histocompatibility complex mhc class ib gene duplications organization and expression patterns in Mouse Strain c57bl 6
BMC Genomics, 2008Co-Authors: Masato Ohtsuka, Hidetoshi Inoko, Jerzy K Kulski, Shinichi YoshimuraAbstract:Background The Mouse has more than 30 Major histocompatibility complex (Mhc) class Ib genes, most of which exist in the H2 region of chromosome 17 in distinct gene clusters. Although recent progress in Mhc research has revealed the unique roles of several Mhc class Ib genes in the immune and non-immune systems, the functions of many class Ib genes have still to be elucidated. To better understand the roles of class Ib molecules, we have characterized their gene duplication, organization and expression patterns within the H2 region of the Mouse Strain C57BL/6.
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major histocompatibility complex mhc class ib gene duplications organization and expression patterns in Mouse Strain c57bl 6
BMC Genomics, 2008Co-Authors: Masato Ohtsuka, Hidetoshi Inoko, Jerzy K Kulski, Shinichi YoshimuraAbstract:The Mouse has more than 30 Major histocompatibility complex (Mhc) class Ib genes, most of which exist in the H2 region of chromosome 17 in distinct gene clusters. Although recent progress in Mhc research has revealed the unique roles of several Mhc class Ib genes in the immune and non-immune systems, the functions of many class Ib genes have still to be elucidated. To better understand the roles of class Ib molecules, we have characterized their gene duplication, organization and expression patterns within the H2 region of the Mouse Strain C57BL/6. The genomic organization of the H2-Q, -T and -M regions was analyzed and 21 transcribed Mhc class Ib genes were identified within these regions. Dot-plot and phylogenetic analyses implied that the genes were generated by monogenic and/or multigenic duplicated events. To investigate the adult tissue, embryonic and placental expressions of these genes, we performed RT-PCR gene expression profiling using gene-specific primers. Both tissue-wide and tissue-specific gene expression patterns were obtained that suggest that the variations in the gene expression may depend on the genomic location of the duplicated genes as well as locus specific mechanisms. The genes located in the H2-T region at the centromeric end of the cluster were expressed more widely than those at the telomeric end, which showed tissue-restricted expression in spite of nucleotide sequence similarities among gene paralogs. Duplicated Mhc class Ib genes located in the H2-Q, -T and -M regions are differentially expressed in a variety of developing and adult tissues. Our findings form the basis for further functional validation studies of the Mhc class Ib gene expression profiles in specific tissues, such as the brain. The duplicated gene expression results in combination with the genome analysis suggest the possibility of long-range regulation of H2-T gene expression and/or important, but as yet unidentified nucleotide changes in the promoter or enhancer regions of the genes. Since the Mhc genomic region has diversified among Mouse Strains, it should be a useful model region for comparative analyses of the relationships between duplicated gene organization, evolution and the regulation of expression patterns.
Hidetoshi Inoko - One of the best experts on this subject based on the ideXlab platform.
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major histocompatibility complex mhc class ib gene duplications organization and expression patterns in Mouse Strain c57bl 6
BMC Genomics, 2008Co-Authors: Masato Ohtsuka, Hidetoshi Inoko, Jerzy K Kulski, Shinichi YoshimuraAbstract:Background The Mouse has more than 30 Major histocompatibility complex (Mhc) class Ib genes, most of which exist in the H2 region of chromosome 17 in distinct gene clusters. Although recent progress in Mhc research has revealed the unique roles of several Mhc class Ib genes in the immune and non-immune systems, the functions of many class Ib genes have still to be elucidated. To better understand the roles of class Ib molecules, we have characterized their gene duplication, organization and expression patterns within the H2 region of the Mouse Strain C57BL/6.
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major histocompatibility complex mhc class ib gene duplications organization and expression patterns in Mouse Strain c57bl 6
BMC Genomics, 2008Co-Authors: Masato Ohtsuka, Hidetoshi Inoko, Jerzy K Kulski, Shinichi YoshimuraAbstract:The Mouse has more than 30 Major histocompatibility complex (Mhc) class Ib genes, most of which exist in the H2 region of chromosome 17 in distinct gene clusters. Although recent progress in Mhc research has revealed the unique roles of several Mhc class Ib genes in the immune and non-immune systems, the functions of many class Ib genes have still to be elucidated. To better understand the roles of class Ib molecules, we have characterized their gene duplication, organization and expression patterns within the H2 region of the Mouse Strain C57BL/6. The genomic organization of the H2-Q, -T and -M regions was analyzed and 21 transcribed Mhc class Ib genes were identified within these regions. Dot-plot and phylogenetic analyses implied that the genes were generated by monogenic and/or multigenic duplicated events. To investigate the adult tissue, embryonic and placental expressions of these genes, we performed RT-PCR gene expression profiling using gene-specific primers. Both tissue-wide and tissue-specific gene expression patterns were obtained that suggest that the variations in the gene expression may depend on the genomic location of the duplicated genes as well as locus specific mechanisms. The genes located in the H2-T region at the centromeric end of the cluster were expressed more widely than those at the telomeric end, which showed tissue-restricted expression in spite of nucleotide sequence similarities among gene paralogs. Duplicated Mhc class Ib genes located in the H2-Q, -T and -M regions are differentially expressed in a variety of developing and adult tissues. Our findings form the basis for further functional validation studies of the Mhc class Ib gene expression profiles in specific tissues, such as the brain. The duplicated gene expression results in combination with the genome analysis suggest the possibility of long-range regulation of H2-T gene expression and/or important, but as yet unidentified nucleotide changes in the promoter or enhancer regions of the genes. Since the Mhc genomic region has diversified among Mouse Strains, it should be a useful model region for comparative analyses of the relationships between duplicated gene organization, evolution and the regulation of expression patterns.
Jerzy K Kulski - One of the best experts on this subject based on the ideXlab platform.
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major histocompatibility complex mhc class ib gene duplications organization and expression patterns in Mouse Strain c57bl 6
BMC Genomics, 2008Co-Authors: Masato Ohtsuka, Hidetoshi Inoko, Jerzy K Kulski, Shinichi YoshimuraAbstract:Background The Mouse has more than 30 Major histocompatibility complex (Mhc) class Ib genes, most of which exist in the H2 region of chromosome 17 in distinct gene clusters. Although recent progress in Mhc research has revealed the unique roles of several Mhc class Ib genes in the immune and non-immune systems, the functions of many class Ib genes have still to be elucidated. To better understand the roles of class Ib molecules, we have characterized their gene duplication, organization and expression patterns within the H2 region of the Mouse Strain C57BL/6.
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major histocompatibility complex mhc class ib gene duplications organization and expression patterns in Mouse Strain c57bl 6
BMC Genomics, 2008Co-Authors: Masato Ohtsuka, Hidetoshi Inoko, Jerzy K Kulski, Shinichi YoshimuraAbstract:The Mouse has more than 30 Major histocompatibility complex (Mhc) class Ib genes, most of which exist in the H2 region of chromosome 17 in distinct gene clusters. Although recent progress in Mhc research has revealed the unique roles of several Mhc class Ib genes in the immune and non-immune systems, the functions of many class Ib genes have still to be elucidated. To better understand the roles of class Ib molecules, we have characterized their gene duplication, organization and expression patterns within the H2 region of the Mouse Strain C57BL/6. The genomic organization of the H2-Q, -T and -M regions was analyzed and 21 transcribed Mhc class Ib genes were identified within these regions. Dot-plot and phylogenetic analyses implied that the genes were generated by monogenic and/or multigenic duplicated events. To investigate the adult tissue, embryonic and placental expressions of these genes, we performed RT-PCR gene expression profiling using gene-specific primers. Both tissue-wide and tissue-specific gene expression patterns were obtained that suggest that the variations in the gene expression may depend on the genomic location of the duplicated genes as well as locus specific mechanisms. The genes located in the H2-T region at the centromeric end of the cluster were expressed more widely than those at the telomeric end, which showed tissue-restricted expression in spite of nucleotide sequence similarities among gene paralogs. Duplicated Mhc class Ib genes located in the H2-Q, -T and -M regions are differentially expressed in a variety of developing and adult tissues. Our findings form the basis for further functional validation studies of the Mhc class Ib gene expression profiles in specific tissues, such as the brain. The duplicated gene expression results in combination with the genome analysis suggest the possibility of long-range regulation of H2-T gene expression and/or important, but as yet unidentified nucleotide changes in the promoter or enhancer regions of the genes. Since the Mhc genomic region has diversified among Mouse Strains, it should be a useful model region for comparative analyses of the relationships between duplicated gene organization, evolution and the regulation of expression patterns.
