The Experts below are selected from a list of 150 Experts worldwide ranked by ideXlab platform
Fernando U. Garcia - One of the best experts on this subject based on the ideXlab platform.
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Mucin Stain on Frozen Sections
Archives of Pathology & Laboratory Medicine, 1999Co-Authors: Suchetha Soans, Lorenzo M. Galindo, Fernando U. GarciaAbstract:Abstract Context.—The importance of frozen-section diagnoses in the practice of pathology cannot be overemphasized. In some cases, the use of a mucin Stain can greatly aid in the diagnosis. Since few methods for mucin Staining have been described that can be used in the frozen-section setting, we developed one such Staining procedure for mucin. Objective.—To develop a rapid Mucicarmine Staining technique to be used on frozen sections that does not significantly delay overall turnaround time. Design.—A standard Mucicarmine Staining technique was modified by using a concentrated Mucicarmine Stain and a microwave oven, to reduce the total Staining time to 3 minutes or less. Frozen tissue from normal colonic mucosa was used as a control, and skin from extramammary Paget disease for evaluation of margins was used as a case. Results.—The rapid Mucicarmine Stain successfully demonstrated the presence of mucin on frozen-tissue sections. Mucin Stained deep rose, and the connective tissue Stained green. Conclusion....
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Mucin Stain on frozen sections: a rapid 3-minute method.
Archives of pathology & laboratory medicine, 1999Co-Authors: Suchetha Soans, Lorenzo M. Galindo, Fernando U. GarciaAbstract:Context.-The importance of frozen-section diagnoses in the practice of pathology cannot be overemphasized. In some cases, the use of a mucin Stain can greatly aid in the diagnosis. Since few methods for mucin Staining have been described that can be used in the frozen-section setting, we developed one such Staining procedure for mucin. Objective.-To develop a rapid Mucicarmine Staining technique to be used on frozen sections that does not significantly delay overall turnaround time. Design.-A standard Mucicarmine Staining technique was modified by using a concentrated Mucicarmine Stain and a microwave oven, to reduce the total Staining time to 3 minutes or less. Frozen tissue from normal colonic mucosa was used as a control, and skin from extramammary Paget disease for evaluation of margins was used as a case. Results.-The rapid Mucicarmine Stain successfully demonstrated the presence of mucin on frozen-tissue sections. Mucin Stained deep rose, and the connective tissue Stained green. Conclusion.-This rapid and simple mucin Staining technique can be used on frozen sections with no significant effect on the overall turnaround time, thereby aiding rapid diagnosis on frozen sections.
Suchetha Soans - One of the best experts on this subject based on the ideXlab platform.
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Mucin Stain on Frozen Sections
Archives of Pathology & Laboratory Medicine, 1999Co-Authors: Suchetha Soans, Lorenzo M. Galindo, Fernando U. GarciaAbstract:Abstract Context.—The importance of frozen-section diagnoses in the practice of pathology cannot be overemphasized. In some cases, the use of a mucin Stain can greatly aid in the diagnosis. Since few methods for mucin Staining have been described that can be used in the frozen-section setting, we developed one such Staining procedure for mucin. Objective.—To develop a rapid Mucicarmine Staining technique to be used on frozen sections that does not significantly delay overall turnaround time. Design.—A standard Mucicarmine Staining technique was modified by using a concentrated Mucicarmine Stain and a microwave oven, to reduce the total Staining time to 3 minutes or less. Frozen tissue from normal colonic mucosa was used as a control, and skin from extramammary Paget disease for evaluation of margins was used as a case. Results.—The rapid Mucicarmine Stain successfully demonstrated the presence of mucin on frozen-tissue sections. Mucin Stained deep rose, and the connective tissue Stained green. Conclusion....
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Mucin Stain on frozen sections: a rapid 3-minute method.
Archives of pathology & laboratory medicine, 1999Co-Authors: Suchetha Soans, Lorenzo M. Galindo, Fernando U. GarciaAbstract:Context.-The importance of frozen-section diagnoses in the practice of pathology cannot be overemphasized. In some cases, the use of a mucin Stain can greatly aid in the diagnosis. Since few methods for mucin Staining have been described that can be used in the frozen-section setting, we developed one such Staining procedure for mucin. Objective.-To develop a rapid Mucicarmine Staining technique to be used on frozen sections that does not significantly delay overall turnaround time. Design.-A standard Mucicarmine Staining technique was modified by using a concentrated Mucicarmine Stain and a microwave oven, to reduce the total Staining time to 3 minutes or less. Frozen tissue from normal colonic mucosa was used as a control, and skin from extramammary Paget disease for evaluation of margins was used as a case. Results.-The rapid Mucicarmine Stain successfully demonstrated the presence of mucin on frozen-tissue sections. Mucin Stained deep rose, and the connective tissue Stained green. Conclusion.-This rapid and simple mucin Staining technique can be used on frozen sections with no significant effect on the overall turnaround time, thereby aiding rapid diagnosis on frozen sections.
Warren R. Selman - One of the best experts on this subject based on the ideXlab platform.
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Mature salivary gland rests within sonic hedgehog-positive medulloblastoma: case report and insights into the molecular genetics and embryopathology of ectopic intracranial salivary gland analogs.
Journal of neurosurgery. Pediatrics, 2016Co-Authors: Berje Shammassian, Sunil Manjila, Kaine Onwuzulike, Dehua Wang, Mark Rodgers, Duncan Stearns, Efrem Cox, Warren R. SelmanAbstract:Intracranial ectopic salivary gland rests within dural-based lesions are reported very infrequently in the literature. The authors report the unique case of a 12-year-old boy with a cerebellar medulloblastoma positive for sonic hedgehog (Shh) that contained intraaxial mature ectopic salivary gland rests. The patient underwent clinical and radiological monitoring postoperatively, until he died of disseminated disease. An autopsy showed no evidence of salivary glands within disseminated lesions. The intraaxial presence of salivary gland rests and concomitant Shh positivity of the described tumor point to a disorder in differentiation as opposed to ectopic developmental foci, which are uniformly dural based in the described literature. The authors demonstrate the characteristic "papilionaceous" appearance of the salivary glands with Mucicarmine Stain and highlight the role of Shh signaling in explaining the intraaxial presence of seromucous gland analogs. This article reports the first intraaxial posterior fossa tumor with heterotopic salivary gland rests, and it provides molecular and embryopathological insights into the development of these lesions.
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Mature salivary gland rests within sonic hedgehog–positive medulloblastoma: case report and insights into the molecular genetics and embryopathology of ectopic intracranial salivary gland analogs
Journal of Neurosurgery: Pediatrics, 2016Co-Authors: Berje Shammassian, Sunil Manjila, Efrem M. Cox, Kaine Onwuzulike, Dehua Wang, Mark Rodgers, Duncan Stearns, Warren R. SelmanAbstract:Intracranial ectopic salivary gland rests within dural-based lesions are reported very infrequently in the literature. The authors report the unique case of a 12-year-old boy with a cerebellar medulloblastoma positive for sonic hedgehog (Shh) that contained intraaxial mature ectopic salivary gland rests. The patient underwent clinical and radiological monitoring postoperatively, until he died of disseminated disease. An autopsy showed no evidence of salivary glands within disseminated lesions. The intraaxial presence of salivary gland rests and concomitant Shh positivity of the described tumor point to a disorder in differentiation as opposed to ectopic developmental foci, which are uniformly dural based in the described literature. The authors demonstrate the characteristic "papilionaceous" appearance of the salivary glands with Mucicarmine Stain and highlight the role of Shh signaling in explaining the intraaxial presence of seromucous gland analogs. This article reports the first intraaxial posterior fossa tumor with heterotopic salivary gland rests, and it provides molecular and embryopathological insights into the development of these lesions.
Lorenzo M. Galindo - One of the best experts on this subject based on the ideXlab platform.
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Mucin Stain on Frozen Sections
Archives of Pathology & Laboratory Medicine, 1999Co-Authors: Suchetha Soans, Lorenzo M. Galindo, Fernando U. GarciaAbstract:Abstract Context.—The importance of frozen-section diagnoses in the practice of pathology cannot be overemphasized. In some cases, the use of a mucin Stain can greatly aid in the diagnosis. Since few methods for mucin Staining have been described that can be used in the frozen-section setting, we developed one such Staining procedure for mucin. Objective.—To develop a rapid Mucicarmine Staining technique to be used on frozen sections that does not significantly delay overall turnaround time. Design.—A standard Mucicarmine Staining technique was modified by using a concentrated Mucicarmine Stain and a microwave oven, to reduce the total Staining time to 3 minutes or less. Frozen tissue from normal colonic mucosa was used as a control, and skin from extramammary Paget disease for evaluation of margins was used as a case. Results.—The rapid Mucicarmine Stain successfully demonstrated the presence of mucin on frozen-tissue sections. Mucin Stained deep rose, and the connective tissue Stained green. Conclusion....
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Mucin Stain on frozen sections: a rapid 3-minute method.
Archives of pathology & laboratory medicine, 1999Co-Authors: Suchetha Soans, Lorenzo M. Galindo, Fernando U. GarciaAbstract:Context.-The importance of frozen-section diagnoses in the practice of pathology cannot be overemphasized. In some cases, the use of a mucin Stain can greatly aid in the diagnosis. Since few methods for mucin Staining have been described that can be used in the frozen-section setting, we developed one such Staining procedure for mucin. Objective.-To develop a rapid Mucicarmine Staining technique to be used on frozen sections that does not significantly delay overall turnaround time. Design.-A standard Mucicarmine Staining technique was modified by using a concentrated Mucicarmine Stain and a microwave oven, to reduce the total Staining time to 3 minutes or less. Frozen tissue from normal colonic mucosa was used as a control, and skin from extramammary Paget disease for evaluation of margins was used as a case. Results.-The rapid Mucicarmine Stain successfully demonstrated the presence of mucin on frozen-tissue sections. Mucin Stained deep rose, and the connective tissue Stained green. Conclusion.-This rapid and simple mucin Staining technique can be used on frozen sections with no significant effect on the overall turnaround time, thereby aiding rapid diagnosis on frozen sections.
Ronaldo Zamuco - One of the best experts on this subject based on the ideXlab platform.
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Microcystic/Reticular Schwannoma Arising in the Submandibular Gland: A Rare Benign Entity that Mimics More Common Salivary Gland Carcinomas
Head and neck pathology, 2015Co-Authors: Ryan P. Lau, Jonathan Melamed, Melissa Yee-chang, Sonya Marcus, Babak Givi, Ronaldo ZamucoAbstract:Microcystic/reticular schwannoma is a recently described variant of schwannoma with a predilection for the gastrointestinal tract, rarely involving the head/neck region. This is the first reported case involving the submandibular gland. We present a case in a 34 year old man with 4.5 cm submandibular mass. Fine needle aspiration suggested a spindle cell lesion. Frozen section evaluation raised the possibility of mucoepidermoid carcinoma. Resection showed a well circumscribed mass with a mucoid appearance. Histologic findings include a lobular architecture with fibrous septa, a lympho-plasmacytic infiltrate, and scattered lymphoid aggregates at the periphery. There are two distinct histologic patterns with solid areas of spindle cells and areas of spindle/ovoid cells with a microcystic pattern in a myxoid background. The tumor has a pushing border, with extension into adipose and adjacent parenchyma, without cytologic atypia or necrosis. Immunohistochemical Stains are positive for S-100 and CD34, and negative for calponin, mammoglobin, ALK1, p63, ER, GFAP, SMA, desmin, cytokeratin 7, cytokeratin AE1/AE3, and C-Kit. Mucicarmine Stain is negative. Recognition of this benign unusual variant of schwannoma is paramount for appropriate conservative treatment due to the morphologic and immunohistochemical overlap with primary salivary gland carcinomas.