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Massimo Lazzeri - One of the best experts on this subject based on the ideXlab platform.
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MP19-15 COMPARATIVE ASSESSMENT OF ORAL AND URETHRAL Mucosa Cell CULTURES AND IN VITRO ANALYSIS OF THEIR REGENERATIVE AND PROLIFERATIVE PROPERTIES FOR TISSUE ENGINEERING URETHRAL RECONSTRUCTION
The Journal of Urology, 2015Co-Authors: Graziella Pellegrini, Francesca Corradini, Guido Barbagli, Michela Zattoni, Michael Giovanardi, Vincenzo Genna, Gianpaolo Bianchi, Massimo LazzeriAbstract:INTRODUCTION AND OBJECTIVES: The success of tissue engineering for urethral reconstruction depends on the quality of the cultures used to prepare the grafts. We investigated the quality and the safety of cultured oral and urethra Mucosa Cells by comparing them, in order to determine the long-term in vitro regenerative properties, the capability to maintain their differentiation program, the heterogeneity of proliferative Cell pool and the differentiation potential clonogenicity. METHODS: Urethral and oral specimens were obtained in accordance with the tenets of the Declaration of Helsinki from 18 patients who underwent urethroplasty with oral Mucosal graft. The primary objectives of the analysis were to determine the Cell yield and migratory capacity of oral and urethral Mucosa Cells, to compare the protein expression, to report the clonogenicity and the long-term proliferative potential. Furthermore we characterised stem Cells of urethral and oral epithelia by holoclones analysis. Finally we investigated the safety of in vitro models regard Cell growth. RESULTS: A statistically significant higher Cell yield was obtained from oral Mucosa than from urethra (t-test p1⁄40,02419), but no significant differences in the number of epithelial clones. Proximity of replicative senescence boosts Cell cycle (faster woundhealing capacity) in oral Mucosal more than in urethra. Keratinocytes from urethral and oral Mucosa underwent a mean of 79.3 and 84 Cell divisions, respectively, before senescence. A similar number of holoclones, meroclones and paraclones were found in oral Mucosal and urethral proliferative compartments. It is shown that a single cultured stem Cell maintains 100% clonogenicity, can be identified by selected markers and can provide differentiation. Analysis of protein expression suggests maintenance of a sitespecific differentiation programme, even under culture conditions. No tumorigenicity or other abnormal Cell growth developed in cultured tissues. CONCLUSIONS: The comparison of all parameters highlighted a wide similarity of the oral and urethral Mucosal Cells, with better wound healing via oral epithelium. The oral Mucosa Cells seem to have a faster wound-healing capacity and a longer replicative survival. This data might have a clinical impact regard the current longer life expectancy of our patients.
Ettore Cardi - One of the best experts on this subject based on the ideXlab platform.
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Cyclic vomiting syndrome: in vitro nitric oxide and interleukin-6 release by esophageal and gastric Mucosa.
Digestive Diseases and Sciences, 2001Co-Authors: Alessandra Zicari, Giovanni Corrado, C. Pacchiarotti, Sandra Lucarelli, Tullio Frediani, Mary Cavaliere, Mariassunta Porcelli, Ettore CardiAbstract:Cyclic vomiting syndrome is a disorder characterized by recurrent episodes of nausea and vomiting with complete resolution of symptoms between attacks. Nitric oxide plays a critical role in regulating several components of gastrointestinal Mucosal defense and injury. Interleukin-6 has a wide variety of actions in the gastrointestinal apparatus. The purpose of this study was to evaluate the synthesis and release of nitric oxide and interleukin-6 by the esophageal and gastric Mucosa in 10 children with cyclic vomiting syndrome, during symptom-free periods, and in 10 controls. The nitric oxide and interleukin-6 release by esophageal Mucosa Cells obtained from cyclic vomiting patients was quite similar to that in controls, but the release of nitric oxide from gastric Mucosa Cells of patients was significantly higher than that of controls. Conversely, no interleukin-6 was detectable in gastric Mucosa Cell supernatants in any of the patients. Further studies are needed to evaluate the relationship between factors triggering cyclic vomiting syndrome and the release of nitric oxide and interleukin-6 by gastric Mucosa.
James L. Bennett - One of the best experts on this subject based on the ideXlab platform.
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Oltipraz Concentrations in Plasma, Buccal Mucosa Cells,and Lipids
Cancer Epidemiology and Prevention Biomarkers, 2001Co-Authors: Nikolay V. Dimitrov, Cheryl Leece, Emily R. Tompkins, Elizabeth Seymour, Maurice R. Bennink, Joseph C. Gardiner, James A. Crowell, Ernest T. Hawk, Mohammed Nashawaty, James L. BennettAbstract:Oltipraz is considered one of the most potent cancer chemoprevention agents, as shown in preclinical studies. Its pharmacological effects in humans have been associated with unusual toxicity affecting the fingers and toes. This study was designed to test intermittent dosing schedules using two dosage levels: 500 mg as a single weekly dose and 200 mg as a biweekly dose, each for 30 days. Fifteen men and women were studied in each dosing group. All were heavy smokers considered to be at high risk for developing lung cancer. Plasma, buccal Mucosa Cell, and lipoprotein concentrations were measured at different intervals corresponding to the time period when most of the adverse effects occur. No serious toxicities were observed using these doses and schedules. The plasma and buccal Mucosa Cell concentrations of Oltipraz showed substantial interindividual variations at each sampling. Some subjects had no detectable plasma or buccal Mucosal Cell Oltipraz concentrations. The distribution of Oltipraz incorporation into the lipid fractions and albumin was changed by the administration of different schedules of Oltipraz. The results of this study suggest that the intermittent dosing is well tolerated and does not result in steady state in plasma or buccal Mucosa Cells. The variation and lack of detectable Oltipraz concentration in plasma, buccal Mucosa Cells, and lipids may affect both the toxicity and the pharmacological effects when these doses and schedules are used.
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Oltipraz concentrations in plasma, buccal Mucosa Cells, and lipids: pharmacological studies.
Cancer epidemiology biomarkers & prevention : a publication of the American Association for Cancer Research cosponsored by the American Society of Pre, 2001Co-Authors: Nikolay V. Dimitrov, Cheryl Leece, Emily R. Tompkins, Elizabeth Seymour, Maurice R. Bennink, Joseph C. Gardiner, James A. Crowell, Ernest T. Hawk, Mohammed Nashawaty, James L. BennettAbstract:Oltipraz is considered one of the most potent cancer chemoprevention agents, as shown in preclinical studies. Its pharmacological effects in humans have been associated with unusual toxicity affecting the fingers and toes. This study was designed to test intermittent dosing schedules using two dosage levels: 500 mg as a single weekly dose and 200 mg as a biweekly dose, each for 30 days. Fifteen men and women were studied in each dosing group. All were heavy smokers considered to be at high risk for developing lung cancer. Plasma, buccal Mucosa Cell, and lipoprotein concentrations were measured at different intervals corresponding to the time period when most of the adverse effects occur. No serious toxicities were observed using these doses and schedules. The plasma and buccal Mucosa Cell concentrations of Oltipraz showed substantial interindividual variations at each sampling. Some subjects had no detectable plasma or buccal Mucosal Cell Oltipraz concentrations. The distribution of Oltipraz incorporation into the lipid fractions and albumin was changed by the administration of different schedules of Oltipraz. The results of this study suggest that the intermittent dosing is well tolerated and does not result in steady state in plasma or buccal Mucosa Cells. The variation and lack of detectable Oltipraz concentration in plasma, buccal Mucosa Cells, and lipids may affect both the toxicity and the pharmacological effects when these doses and schedules are used.
Robin J.m. Franklin - One of the best experts on this subject based on the ideXlab platform.
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Contrasting effects of basic fibroblast growth factor and epidermal growth factor on mouse neonatal olfactory Mucosa Cells.
The European journal of neuroscience, 2007Co-Authors: Perrine Barraud, Chao Zhao, Chrystelle Ibanez, R. Raha-chowdhury, Maeve A. Caldwell, Robin J.m. FranklinAbstract:Basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) affect proliferation and survival of many Cell types, but their role in the maintenance of olfactory Mucosa Cells remains unclear. In the neonatal mouse olfactory Mucosa, Cell proliferation mainly occurs in the neuroepithelium and, to a lesser extent, in the lamina propria. To establish whether bFGF and EGF affect proliferation and/or survival of these Cells, we isolated olfactory Mucosa Cells from the neonatal mouse and cultured them as free-floating spheres under bFGF or EGF stimulation. Our data demonstrate that bFGF is a mitogen for the rapidly dividing Cells (olfactory neuronal precursors and olfactory ensheathing Cells), and also a survival factor for both slowly and rapidly dividing Cells of the olfactory Mucosa. In contrast, EGF appears to be primarily a survival factor for both the olfactory stem and precursor Cells.
Graziella Pellegrini - One of the best experts on this subject based on the ideXlab platform.
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MP19-15 COMPARATIVE ASSESSMENT OF ORAL AND URETHRAL Mucosa Cell CULTURES AND IN VITRO ANALYSIS OF THEIR REGENERATIVE AND PROLIFERATIVE PROPERTIES FOR TISSUE ENGINEERING URETHRAL RECONSTRUCTION
The Journal of Urology, 2015Co-Authors: Graziella Pellegrini, Francesca Corradini, Guido Barbagli, Michela Zattoni, Michael Giovanardi, Vincenzo Genna, Gianpaolo Bianchi, Massimo LazzeriAbstract:INTRODUCTION AND OBJECTIVES: The success of tissue engineering for urethral reconstruction depends on the quality of the cultures used to prepare the grafts. We investigated the quality and the safety of cultured oral and urethra Mucosa Cells by comparing them, in order to determine the long-term in vitro regenerative properties, the capability to maintain their differentiation program, the heterogeneity of proliferative Cell pool and the differentiation potential clonogenicity. METHODS: Urethral and oral specimens were obtained in accordance with the tenets of the Declaration of Helsinki from 18 patients who underwent urethroplasty with oral Mucosal graft. The primary objectives of the analysis were to determine the Cell yield and migratory capacity of oral and urethral Mucosa Cells, to compare the protein expression, to report the clonogenicity and the long-term proliferative potential. Furthermore we characterised stem Cells of urethral and oral epithelia by holoclones analysis. Finally we investigated the safety of in vitro models regard Cell growth. RESULTS: A statistically significant higher Cell yield was obtained from oral Mucosa than from urethra (t-test p1⁄40,02419), but no significant differences in the number of epithelial clones. Proximity of replicative senescence boosts Cell cycle (faster woundhealing capacity) in oral Mucosal more than in urethra. Keratinocytes from urethral and oral Mucosa underwent a mean of 79.3 and 84 Cell divisions, respectively, before senescence. A similar number of holoclones, meroclones and paraclones were found in oral Mucosal and urethral proliferative compartments. It is shown that a single cultured stem Cell maintains 100% clonogenicity, can be identified by selected markers and can provide differentiation. Analysis of protein expression suggests maintenance of a sitespecific differentiation programme, even under culture conditions. No tumorigenicity or other abnormal Cell growth developed in cultured tissues. CONCLUSIONS: The comparison of all parameters highlighted a wide similarity of the oral and urethral Mucosal Cells, with better wound healing via oral epithelium. The oral Mucosa Cells seem to have a faster wound-healing capacity and a longer replicative survival. This data might have a clinical impact regard the current longer life expectancy of our patients.