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Thomas Preat - One of the best experts on this subject based on the ideXlab platform.
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Upregulated energy metabolism in the Drosophila Mushroom Body is the trigger for long-term memory.
Nature communications, 2017Co-Authors: Pierreyves Placais, Eloise De Tredern, Lisa Scheunemann, Severine Trannoy, Valerie Goguel, Kyung An Han, Guillaume Isabel, Thomas PreatAbstract:Efficient energy use has constrained the evolution of nervous systems. However, it is unresolved whether energy metabolism may resultantly regulate major brain functions. Our observation that Drosophila flies double their sucrose intake at an early stage of long-term memory formation initiated the investigation of how energy metabolism intervenes in this process. Cellular-resolution imaging of energy metabolism reveals a concurrent elevation of energy consumption in neurons of the Mushroom Body, the fly's major memory centre. Strikingly, upregulation of Mushroom Body energy flux is both necessary and sufficient to drive long-term memory formation. This effect is triggered by a specific pair of dopaminergic neurons afferent to the Mushroom bodies, via the D5-like DAMB dopamine receptor. Hence, dopamine signalling mediates an energy switch in the Mushroom Body that controls long-term memory encoding. Our data thus point to an instructional role for energy flux in the execution of demanding higher brain functions.
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upregulated energy metabolism in the drosophila Mushroom Body is the trigger for long term memory
Nature Communications, 2017Co-Authors: Pierreyves Placais, Eloise De Tredern, Lisa Scheunemann, Severine Trannoy, Valerie Goguel, Kyung An Han, Guillaume Isabel, Thomas PreatAbstract:Efficient energy use has constrained the evolution of nervous systems. However, it is unresolved whether energy metabolism may resultantly regulate major brain functions. Our observation that Drosophila flies double their sucrose intake at an early stage of long-term memory formation initiated the investigation of how energy metabolism intervenes in this process. Cellular-resolution imaging of energy metabolism reveals a concurrent elevation of energy consumption in neurons of the Mushroom Body, the fly’s major memory centre. Strikingly, upregulation of Mushroom Body energy flux is both necessary and sufficient to drive long-term memory formation. This effect is triggered by a specific pair of dopaminergic neurons afferent to the Mushroom bodies, via the D5-like DAMB dopamine receptor. Hence, dopamine signalling mediates an energy switch in the Mushroom Body that controls long-term memory encoding. Our data thus point to an instructional role for energy flux in the execution of demanding higher brain functions. Energy consumption in the brain is thought to respond to changes in neuronal activity, without informational role. Here the authors show that increased energy flux in the Mushroom Body, driven by a pair of input dopaminergic neurons, is a command for the formation of long-term memory in Drosophila.
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two independent Mushroom Body output circuits retrieve the six discrete components of drosophila aversive memory
Cell Reports, 2015Co-Authors: Emna Bouzaiane, Lisa Scheunemann, Pierreyves Placais, Severine Trannoy, Thomas PreatAbstract:Understanding how the various memory components are encoded and how they interact to guide behavior requires knowledge of the underlying neural circuits. Currently, aversive olfactory memory in Drosophila is behaviorally subdivided into four discrete phases. Among these, short- and long-term memories rely, respectively, on the γ and α/β Kenyon cells (KCs), two distinct subsets of the ∼2,000 neurons in the Mushroom Body (MB). Whereas V2 efferent neurons retrieve memory from α/β KCs, the neurons that retrieve short-term memory are unknown. We identified a specific pair of MB efferent neurons, named M6, that retrieve memory from γ KCs. Moreover, our network analysis revealed that six discrete memory phases actually exist, three of which have been conflated in the past. At each time point, two distinct memory components separately recruit either V2 or M6 output pathways. Memory retrieval thus features a dramatic convergence from KCs to MB efferent neurons.
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Localization of Long-Term Memory Within the Drosophila Mushroom Body
Science (New York N.Y.), 2001Co-Authors: Alberto Pascual, Thomas PreatAbstract:The Mushroom bodies, substructures of the Drosophila brain, are involved in olfactory learning and short-term memory, but their role in long-term memory is unknown. Here we show that the alpha-lobes-absent (ala) mutant lacks either the two vertical lobes of the Mushroom Body or two of the three median lobes which contain branches of vertical lobe neurons. This unique phenotype allows analysis of Mushroom Body function. Long-term memory required the presence of the vertical lobes but not the median lobes. Short-term memory was normal in flies without either vertical lobes or the two median lobes studied.
Pierreyves Placais - One of the best experts on this subject based on the ideXlab platform.
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upregulated energy metabolism in the drosophila Mushroom Body is the trigger for long term memory
Nature Communications, 2017Co-Authors: Pierreyves Placais, Eloise De Tredern, Lisa Scheunemann, Severine Trannoy, Valerie Goguel, Kyung An Han, Guillaume Isabel, Thomas PreatAbstract:Efficient energy use has constrained the evolution of nervous systems. However, it is unresolved whether energy metabolism may resultantly regulate major brain functions. Our observation that Drosophila flies double their sucrose intake at an early stage of long-term memory formation initiated the investigation of how energy metabolism intervenes in this process. Cellular-resolution imaging of energy metabolism reveals a concurrent elevation of energy consumption in neurons of the Mushroom Body, the fly’s major memory centre. Strikingly, upregulation of Mushroom Body energy flux is both necessary and sufficient to drive long-term memory formation. This effect is triggered by a specific pair of dopaminergic neurons afferent to the Mushroom bodies, via the D5-like DAMB dopamine receptor. Hence, dopamine signalling mediates an energy switch in the Mushroom Body that controls long-term memory encoding. Our data thus point to an instructional role for energy flux in the execution of demanding higher brain functions. Energy consumption in the brain is thought to respond to changes in neuronal activity, without informational role. Here the authors show that increased energy flux in the Mushroom Body, driven by a pair of input dopaminergic neurons, is a command for the formation of long-term memory in Drosophila.
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Upregulated energy metabolism in the Drosophila Mushroom Body is the trigger for long-term memory.
Nature communications, 2017Co-Authors: Pierreyves Placais, Eloise De Tredern, Lisa Scheunemann, Severine Trannoy, Valerie Goguel, Kyung An Han, Guillaume Isabel, Thomas PreatAbstract:Efficient energy use has constrained the evolution of nervous systems. However, it is unresolved whether energy metabolism may resultantly regulate major brain functions. Our observation that Drosophila flies double their sucrose intake at an early stage of long-term memory formation initiated the investigation of how energy metabolism intervenes in this process. Cellular-resolution imaging of energy metabolism reveals a concurrent elevation of energy consumption in neurons of the Mushroom Body, the fly's major memory centre. Strikingly, upregulation of Mushroom Body energy flux is both necessary and sufficient to drive long-term memory formation. This effect is triggered by a specific pair of dopaminergic neurons afferent to the Mushroom bodies, via the D5-like DAMB dopamine receptor. Hence, dopamine signalling mediates an energy switch in the Mushroom Body that controls long-term memory encoding. Our data thus point to an instructional role for energy flux in the execution of demanding higher brain functions.
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two independent Mushroom Body output circuits retrieve the six discrete components of drosophila aversive memory
Cell Reports, 2015Co-Authors: Emna Bouzaiane, Lisa Scheunemann, Pierreyves Placais, Severine Trannoy, Thomas PreatAbstract:Understanding how the various memory components are encoded and how they interact to guide behavior requires knowledge of the underlying neural circuits. Currently, aversive olfactory memory in Drosophila is behaviorally subdivided into four discrete phases. Among these, short- and long-term memories rely, respectively, on the γ and α/β Kenyon cells (KCs), two distinct subsets of the ∼2,000 neurons in the Mushroom Body (MB). Whereas V2 efferent neurons retrieve memory from α/β KCs, the neurons that retrieve short-term memory are unknown. We identified a specific pair of MB efferent neurons, named M6, that retrieve memory from γ KCs. Moreover, our network analysis revealed that six discrete memory phases actually exist, three of which have been conflated in the past. At each time point, two distinct memory components separately recruit either V2 or M6 output pathways. Memory retrieval thus features a dramatic convergence from KCs to MB efferent neurons.
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Mushroom Body output neurons encode valence and guide memory based action selection in drosophila
eLife, 2014Co-Authors: Yoshinori Aso, Nobuhiro Yamagata, Karla R. Kaun, Pierreyves Placais, Divya Sitaraman, Katrin Vogt, Toshiharu Ichinose, Ghislain Belliartguerin, Alice A Robie, Christopher SchnaitmannAbstract:Animals discriminate stimuli, learn their predictive value and use this knowledge to modify their behavior. In Drosophila, the Mushroom Body (MB) plays a key role in these processes. Sensory stimuli are sparsely represented by ∼2000 Kenyon cells, which converge onto 34 output neurons (MBONs) of 21 types. We studied the role of MBONs in several associative learning tasks and in sleep regulation, revealing the extent to which information flow is segregated into distinct channels and suggesting possible roles for the multi-layered MBON network. We also show that optogenetic activation of MBONs can, depending on cell type, induce repulsion or attraction in flies. The behavioral effects of MBON perturbation are combinatorial, suggesting that the MBON ensemble collectively represents valence. We propose that local, stimulus-specific dopaminergic modulation selectively alters the balance within the MBON network for those stimuli. Our results suggest that valence encoded by the MBON ensemble biases memory-based action selection.
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Mushroom Body efferent neurons responsible for aversive olfactory memory retrieval in drosophila
Nature Neuroscience, 2011Co-Authors: Julien Sejourne, Yoshinori Aso, Igor Siwanowicz, Pierreyves Placais, Severine Trannoy, Vladimiros Thoma, Stevanus Rio Tedjakumala, Gerald M Rubin, P Tchenio, Kei ItoAbstract:This study reports an anatomical and functional screen of Mushroom Body–extrinsic neurons in Drosophila and finds that MB-V2 cholinergic efferent neurons are essential for retrieval of aversive short- and long-term memory, but not for memory formation or consolidation. During memory retrieval, MB-V2 neurons reinforce the olfactory pathway involved in innate odor avoidance.
Severine Trannoy - One of the best experts on this subject based on the ideXlab platform.
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upregulated energy metabolism in the drosophila Mushroom Body is the trigger for long term memory
Nature Communications, 2017Co-Authors: Pierreyves Placais, Eloise De Tredern, Lisa Scheunemann, Severine Trannoy, Valerie Goguel, Kyung An Han, Guillaume Isabel, Thomas PreatAbstract:Efficient energy use has constrained the evolution of nervous systems. However, it is unresolved whether energy metabolism may resultantly regulate major brain functions. Our observation that Drosophila flies double their sucrose intake at an early stage of long-term memory formation initiated the investigation of how energy metabolism intervenes in this process. Cellular-resolution imaging of energy metabolism reveals a concurrent elevation of energy consumption in neurons of the Mushroom Body, the fly’s major memory centre. Strikingly, upregulation of Mushroom Body energy flux is both necessary and sufficient to drive long-term memory formation. This effect is triggered by a specific pair of dopaminergic neurons afferent to the Mushroom bodies, via the D5-like DAMB dopamine receptor. Hence, dopamine signalling mediates an energy switch in the Mushroom Body that controls long-term memory encoding. Our data thus point to an instructional role for energy flux in the execution of demanding higher brain functions. Energy consumption in the brain is thought to respond to changes in neuronal activity, without informational role. Here the authors show that increased energy flux in the Mushroom Body, driven by a pair of input dopaminergic neurons, is a command for the formation of long-term memory in Drosophila.
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Upregulated energy metabolism in the Drosophila Mushroom Body is the trigger for long-term memory.
Nature communications, 2017Co-Authors: Pierreyves Placais, Eloise De Tredern, Lisa Scheunemann, Severine Trannoy, Valerie Goguel, Kyung An Han, Guillaume Isabel, Thomas PreatAbstract:Efficient energy use has constrained the evolution of nervous systems. However, it is unresolved whether energy metabolism may resultantly regulate major brain functions. Our observation that Drosophila flies double their sucrose intake at an early stage of long-term memory formation initiated the investigation of how energy metabolism intervenes in this process. Cellular-resolution imaging of energy metabolism reveals a concurrent elevation of energy consumption in neurons of the Mushroom Body, the fly's major memory centre. Strikingly, upregulation of Mushroom Body energy flux is both necessary and sufficient to drive long-term memory formation. This effect is triggered by a specific pair of dopaminergic neurons afferent to the Mushroom bodies, via the D5-like DAMB dopamine receptor. Hence, dopamine signalling mediates an energy switch in the Mushroom Body that controls long-term memory encoding. Our data thus point to an instructional role for energy flux in the execution of demanding higher brain functions.
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two independent Mushroom Body output circuits retrieve the six discrete components of drosophila aversive memory
Cell Reports, 2015Co-Authors: Emna Bouzaiane, Lisa Scheunemann, Pierreyves Placais, Severine Trannoy, Thomas PreatAbstract:Understanding how the various memory components are encoded and how they interact to guide behavior requires knowledge of the underlying neural circuits. Currently, aversive olfactory memory in Drosophila is behaviorally subdivided into four discrete phases. Among these, short- and long-term memories rely, respectively, on the γ and α/β Kenyon cells (KCs), two distinct subsets of the ∼2,000 neurons in the Mushroom Body (MB). Whereas V2 efferent neurons retrieve memory from α/β KCs, the neurons that retrieve short-term memory are unknown. We identified a specific pair of MB efferent neurons, named M6, that retrieve memory from γ KCs. Moreover, our network analysis revealed that six discrete memory phases actually exist, three of which have been conflated in the past. At each time point, two distinct memory components separately recruit either V2 or M6 output pathways. Memory retrieval thus features a dramatic convergence from KCs to MB efferent neurons.
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Mushroom Body efferent neurons responsible for aversive olfactory memory retrieval in drosophila
Nature Neuroscience, 2011Co-Authors: Julien Sejourne, Yoshinori Aso, Igor Siwanowicz, Pierreyves Placais, Severine Trannoy, Vladimiros Thoma, Stevanus Rio Tedjakumala, Gerald M Rubin, P Tchenio, Kei ItoAbstract:This study reports an anatomical and functional screen of Mushroom Body–extrinsic neurons in Drosophila and finds that MB-V2 cholinergic efferent neurons are essential for retrieval of aversive short- and long-term memory, but not for memory formation or consolidation. During memory retrieval, MB-V2 neurons reinforce the olfactory pathway involved in innate odor avoidance.
Yoshinori Aso - One of the best experts on this subject based on the ideXlab platform.
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transsynaptic mapping of drosophila Mushroom Body output neurons
eLife, 2021Co-Authors: Kristin M. Scaplen, Mustafa Talay, John D. Fisher, Raphael Cohn, Altar Sorkaç, Yoshinori Aso, Gilad Barnea, Karla R. KaunAbstract:The Mushroom Body (MB) is a well-characterized associative memory structure within the Drosophila brain. Analyzing MB connectivity using multiple approaches is critical for understanding the functional implications of this structure. Using the genetic anterograde transsynaptic tracing tool, trans-Tango, we identified divergent projections across the brain and convergent downstream targets of the MB output neurons (MBONs). Our analysis revealed at least three separate targets that receive convergent input from MBONs: other MBONs, the fan-shaped Body (FSB), and the lateral accessory lobe (LAL). We describe, both anatomically and functionally, a multilayer circuit in which inhibitory and excitatory MBONs converge on the same genetic subset of FSB and LAL neurons. This circuit architecture enables the brain to update and integrate information with previous experience before executing appropriate behavioral responses. Our use of trans-Tango provides a genetically accessible anatomical framework for investigating the functional relevance of components within these complex and interconnected circuits.
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Transsynaptic mapping of Drosophila Mushroom Body output neurons
2020Co-Authors: Kristin M. Scaplen, Mustafa Talay, John D. Fisher, Raphael Cohn, Altar Sorkaç, Yoshinori Aso, Gilad Barnea, Karla R. KaunAbstract:AbstractThe Mushroom Body (MB) is a well-characterized associative memory structure within the Drosophila brain. Although previous studies have analyzed MB connectivity and provided a map of inputs and outputs, a detailed map of the downstream targets is missing. Using the genetic anterograde transsynaptic tracing tool, trans-Tango, we identified divergent projections across the brain and convergent downstream targets of the MB output neurons (MBONs). Our analysis revealed at least three separate targets that receive convergent input from MBONs: other MBONs, the fan shaped Body (FSB), and the lateral accessory lobe (LAL). We describe, both anatomically and functionally, a multilayer circuit in which inhibitory and excitatory MBONs converge on the same genetic subset of FSB and LAL neurons. This circuit architecture provides an opportunity for the brain to update information and integrate it with previous experience before executing appropriate behavioral responses.Highlights-The postsynaptic connections of the output neurons of the Mushroom Body, a structure that integrates environmental cues with associated valence, are mapped using trans-Tango.-Mushroom Body circuits are highly interconnected with several points of convergence among Mushroom Body output neurons (MBONs).-The postsynaptic partners of MBONs have divergent projections across the brain and convergent projections to select target neuropils outside the Mushroom Body important for multimodal integration.-Functional connectivity suggests the presence of multisynaptic pathways that have several layers of integration prior to initiation of an output response.
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transsynaptic mapping of drosophila Mushroom Body output neurons
bioRxiv, 2020Co-Authors: Kristin M. Scaplen, Mustafa Talay, John D. Fisher, Raphael Cohn, Altar Sorkaç, Yoshinori Aso, Gilad Barnea, Karla R. KaunAbstract:The Mushroom Body (MB) is a well-characterized associative memory structure within the Drosophila brain. Although previous studies have analyzed MB connectivity and provided a map of inputs and outputs, a detailed map of the downstream targets is missing. Using the genetic anterograde transsynaptic tracing tool, trans-Tango, we identified divergent projections across the brain and convergent downstream targets of the MB output neurons (MBONs). Our analysis revealed at least three separate targets that receive convergent input from MBONs: other MBONs, the fan shaped Body (FSB), and the lateral accessory lobe (LAL). We describe, both anatomically and functionally, a multilayer circuit in which inhibitory and excitatory MBONs converge on the same genetic subset of FSB and LAL neurons. This circuit architecture provides an opportunity for the brain to update information and integrate it with previous experience before executing appropriate behavioral responses.
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Functional architecture of reward learning in Mushroom Body extrinsic neurons of larval Drosophila.
Nature communications, 2018Co-Authors: Timo Saumweber, Yoshinori Aso, Astrid Rohwedder, Michael Schleyer, Katharina Eichler, Yi-chun Chen, Albert Cardona, Claire Eschbach, Oliver Kobler, Anne VoigtAbstract:The brain adaptively integrates present sensory input, past experience, and options for future action. The insect Mushroom Body exemplifies how a central brain structure brings about such integration. Here we use a combination of systematic single-cell labeling, connectomics, transgenic silencing, and activation experiments to study the Mushroom Body at single-cell resolution, focusing on the behavioral architecture of its input and output neurons (MBINs and MBONs), and of the Mushroom Body intrinsic APL neuron. Our results reveal the identity and morphology of almost all of these 44 neurons in stage 3 Drosophila larvae. Upon an initial screen, functional analyses focusing on the Mushroom Body medial lobe uncover sparse and specific functions of its dopaminergic MBINs, its MBONs, and of the GABAergic APL neuron across three behavioral tasks, namely odor preference, taste preference, and associative learning between odor and taste. Our results thus provide a cellular-resolution study case of how brains organize behavior.
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representations of novelty and familiarity in a Mushroom Body compartment
Cell, 2017Co-Authors: Daisuke Hattori, Yoshinori Aso, L. F. Abbott, Gerald M Rubin, Kurtis J Swartz, Richard AxelAbstract:Animals exhibit a behavioral response to novel sensory stimuli about which they have no prior knowledge. We have examined the neural and behavioral correlates of novelty and familiarity in the olfactory system of Drosophila. Novel odors elicit strong activity in output neurons (MBONs) of the α′3 compartment of the Mushroom Body that is rapidly suppressed upon repeated exposure to the same odor. This transition in neural activity upon familiarization requires odor-evoked activity in the dopaminergic neuron innervating this compartment. Moreover, exposure of a fly to novel odors evokes an alerting response that can also be elicited by optogenetic activation of α′3 MBONs. Silencing these MBONs eliminates the alerting behavior. These data suggest that the α′3 compartment plays a causal role in the behavioral response to novel and familiar stimuli as a consequence of dopamine-mediated plasticity at the Kenyon cell-MBONα′3 synapse.
Guillaume Isabel - One of the best experts on this subject based on the ideXlab platform.
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upregulated energy metabolism in the drosophila Mushroom Body is the trigger for long term memory
Nature Communications, 2017Co-Authors: Pierreyves Placais, Eloise De Tredern, Lisa Scheunemann, Severine Trannoy, Valerie Goguel, Kyung An Han, Guillaume Isabel, Thomas PreatAbstract:Efficient energy use has constrained the evolution of nervous systems. However, it is unresolved whether energy metabolism may resultantly regulate major brain functions. Our observation that Drosophila flies double their sucrose intake at an early stage of long-term memory formation initiated the investigation of how energy metabolism intervenes in this process. Cellular-resolution imaging of energy metabolism reveals a concurrent elevation of energy consumption in neurons of the Mushroom Body, the fly’s major memory centre. Strikingly, upregulation of Mushroom Body energy flux is both necessary and sufficient to drive long-term memory formation. This effect is triggered by a specific pair of dopaminergic neurons afferent to the Mushroom bodies, via the D5-like DAMB dopamine receptor. Hence, dopamine signalling mediates an energy switch in the Mushroom Body that controls long-term memory encoding. Our data thus point to an instructional role for energy flux in the execution of demanding higher brain functions. Energy consumption in the brain is thought to respond to changes in neuronal activity, without informational role. Here the authors show that increased energy flux in the Mushroom Body, driven by a pair of input dopaminergic neurons, is a command for the formation of long-term memory in Drosophila.
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Upregulated energy metabolism in the Drosophila Mushroom Body is the trigger for long-term memory.
Nature communications, 2017Co-Authors: Pierreyves Placais, Eloise De Tredern, Lisa Scheunemann, Severine Trannoy, Valerie Goguel, Kyung An Han, Guillaume Isabel, Thomas PreatAbstract:Efficient energy use has constrained the evolution of nervous systems. However, it is unresolved whether energy metabolism may resultantly regulate major brain functions. Our observation that Drosophila flies double their sucrose intake at an early stage of long-term memory formation initiated the investigation of how energy metabolism intervenes in this process. Cellular-resolution imaging of energy metabolism reveals a concurrent elevation of energy consumption in neurons of the Mushroom Body, the fly's major memory centre. Strikingly, upregulation of Mushroom Body energy flux is both necessary and sufficient to drive long-term memory formation. This effect is triggered by a specific pair of dopaminergic neurons afferent to the Mushroom bodies, via the D5-like DAMB dopamine receptor. Hence, dopamine signalling mediates an energy switch in the Mushroom Body that controls long-term memory encoding. Our data thus point to an instructional role for energy flux in the execution of demanding higher brain functions.
