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Norbert Mencke - One of the best experts on this subject based on the ideXlab platform.
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establishment of the cat flea ctenocephalides felis felis on the ferret Mustela Putorius Furo and its control with imidacloprid
Medical and Veterinary Entomology, 2001Co-Authors: M J Hutchinson, D E Jacobs, Norbert MenckeAbstract:As the ferret, Mustela Putorius Furo L. (Carnivora: Mustelidae), is becoming increasingly popular as a pet animal and as it is susceptible to the cat-flea, Ctenocephalides felis felis Bouche (Siphonaptera: Pulicidae), an experimental model was established for evaluating insecticidal treatments on this host. A high establishment rate (76.7-91.8%) was recorded when 60 unfed adult C. felis were placed on ferrets. This provided an adequate infestation for chemotherapeutic evaluation without causing undue discomfort to the host. Twelve ferrets were allocated to two groups matched for sex and individual ability to sustain a flea population. One group was treated topically with an imidacloprid spot-on formulation at a dose rate of 10 mg/kg body-weight on Day 0. All ferrets were infested with C. felis on Days -1, 7, 14, 21 and 28, and flea counts were performed 8 and 24 h post-treatment and one day after each subsequent infestation. Fleas were removed at all but the 8 h count (when they were returned to their host). Flea burdens were reduced by 95.3% (P < 0.001) within 8 h of treatment and 100% efficacy was recorded at 24 h. At 1, 2, 3 and 4 weeks post-treatment, protection against re-infestation was 92.9% (P < 0.001), 55.7% (P < 0.02), 18.3% (NS) and 7.4% (NS), respectively. Thus, at this dose rate, imidacloprid gave excellent efficacy against a resident C. felis population and provided a high level of residual activity for at least one week after treatment.
Kathie Burkett - One of the best experts on this subject based on the ideXlab platform.
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in vitro characterisation of sars cov 2 and susceptibility of domestic ferrets Mustela Putorius Furo
Transboundary and Emerging Diseases, 2021Co-Authors: Glenn A Marsh, Alexander J Mcauley, Sheree Brown, Elizabeth A Pharo, Sandra Crameri, Michelle L Baker, Jennifer A Barr, Jemma Bergfeld, Matthew P Bruce, Kathie BurkettAbstract:Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an emerging virus that has caused significant human morbidity and mortality since its detection in late 2019. With the rapid emergence has come an unprecedented programme of vaccine development with at least 300 candidates under development. Ferrets have proven to be an appropriate animal model for testing safety and efficacy of SARS-CoV-2 vaccines due to quantifiable virus shedding in nasal washes and oral swabs. Here, we outline our efforts early in the SARS-CoV-2 outbreak to propagate and characterize an Australian isolate of the virus in vitro and in an ex vivo model of human airway epithelium, as well as to demonstrate the susceptibility of domestic ferrets (Mustela Putorius Furo) to SARS-CoV-2 infection following intranasal challenge.
Jacobo Giner - One of the best experts on this subject based on the ideXlab platform.
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antibodies to leishmania in naturally exposed domestic ferrets Mustela Putorius Furo in spain
Veterinary Parasitology, 2021Co-Authors: Sergio Villanuevasaz, Jacobo Giner, M M Alcover, Cristina Riera, Roser Fisa, M T Verde, Andres Yzuel, Hector Ruiz, D Lacasta, A FernandezAbstract:Abstract Zoonotic leishmaniosis due to Leishmania infantum is a vector-borne disease endemic in southern Europe and dogs are the main reservoir for this infection. Seasonal variations in antibody titers in this species in areas where phlebotomine vectors have seasonal patterns of activity are important for epidemiological, preventive and clinical studies related with canine leishmaniosis. It has been suggested that cats, rabbits and ferrets may act as peridomestic reservoirs and not only as accidental hosts. The aim of this study was to determine if seropositive ferrets (Mustela Putorius Furo) to Leishmania could be affected by seasonal variations of anti-Leishmania antibodies. A group of seropositive clinically healthy ferrets (n = 21) were included in this study. A significant reduction in anti-Leishmania infantum antibodies was detected during non-transmission period (December 2020-February 2021) in comparison to transmission period (April-October 2020). This study describes for the first time a seasonal variation in the anti-Leishmania antibodies detected in domestic ferrets following natural exposure during sand fly transmission period and the following non-sand fly transmission period in a Mediterranean area considered as an area where L. infantum is endemic.
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clinical leishmaniosis in a domestic ferret Mustela Putorius Furo treated with miltefosine plus allopurinol serological and clinical follow up
Veterinary Parasitology: Regional Studies and Reports, 2021Co-Authors: Jacobo Giner, M M Alcover, Cristina Riera, Roser Fisa, M T Verde, Sergio Villanuevasaz, A Fernandez, Andres YzuelAbstract:Abstract The published information on the treatment of mustelid leishmaniosis is extremely scarce because there are only two case reports available. In one case, a domestic ferret (Mustela Putorius Furo) was treated with a combination of meglumine antimoniate plus allopurinol and, in the other case, a therapeutic regimen with allopurinol was administrated to a Eurasian otter (Lutra lutra). This article describes for the first time a combined therapeutic protocol with miltefosine (2 mg/kg once a day during 28 days per os), and allopurinol (10 mg/kg twice a day PO sine die) in a domestic ferret with splenomegaly, lymphadenomegaly and a facial pyogranulomatous dermatitis, with a moderate level of antibodies to Leishmania infantum.
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sars cov 2 seroprevalence in household domestic ferrets Mustela Putorius Furo
Animal, 2021Co-Authors: Jacobo Giner, M T Verde, Andres Yzuel, Sergio Villanuevasaz, Ana Pilar Tobajas, M Perez, Ana Gonzalez, Ana Garciagarcia, Victor Taleb, Erandi LiranavarreteAbstract:Animal infections with SARS-CoV-2 have been reported in different countries and several animal species have been proven to be susceptible to infection with SARS-CoV-2 both naturally and by experimental infection. Moreover, infections under natural conditions in more than 20 mink farms have been reported where humans could have been the source of infection for minks. However, little information is available about the susceptibility of pet animals under natural conditions and currently there is no SARS-CoV-2 epidemiological assessment occurrence in household ferrets. In this study, the presence of SARS-CoV-2 antibodies was evaluated in serum samples obtained from 127 household ferrets (Mustela Putorius Furo) in the Province of Valencia (Spain). Two ferrets tested positive to SARS-CoV-2 (1.57%) by in-house enzyme-linked immunosorbent assay based on receptor binding domain (RBD) of Spike antigen. Furthermore, anti-RBD SARS-CoV-2 antibodies persisted at detectable levels in a seropositive SARS-CoV-2 domestic ferret beyond 129 days since the first time antibodies were detected. This study reports for the first time the evidence of household pet ferrets exposure to SARS-CoV-2 in Spain to date.
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first report on natural infection with leishmania infantum in a domestic ferret Mustela Putorius Furo in spain
Veterinary Parasitology: Regional Studies and Reports, 2020Co-Authors: Jacobo Giner, Asier Basurco, M M Alcover, Cristina Riera, Roser Fisa, R A Lopez, C Juansalles, M T Verde, Antonio Fernandez, Andres YzuelAbstract:A pet domestic ferret (Mustela Putorius Furo) with a papular lesion involving the right pinna was diagnosed with chronic pyogranulomatous dermatitis by histopathologic examination. Intralesional, intracytoplasmic oval microorganisms compatible with Leishmania spp. or Histoplasma spp. were observed in macrophages and multinucleate giant cells. Leishmania infantum (L. infantum) infection was diagnosed by PCR, culture in Novy-MacNeal-Nicolle medium, and immunohistochemistry. Abnormal clinicopathological results included increased alanine transferase, alkaline phosphatase, serum gamma glutamyl transferase and polyclonal gammpathy. Anti-Leishmania antibodies were detected by enzyme-linked immunosorbent assay, immunofluorescence antibody test and western blot using L. infantum antigen. Immunoreactivity against the 16 kDa specific L. infantum antigen fraction was observed by western blot. PCR performed in blood samples obtained from this patient after positive parasite isolation detected L. infantum DNA. To the authors' knowledge, this is the first diagnosis and isolation of L. infantum in a domestic ferret naturally infected in an endemic region (Spain) where canine and feline leishmaniosis is frequently detected. According to these findings, ferrets should be included as potential reservoir hosts of L. infantum. Future investigations should analyze the epidemiological role of ferrets in L. infantum infection including the prevalence of infection.
Andres Yzuel - One of the best experts on this subject based on the ideXlab platform.
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antibodies to leishmania in naturally exposed domestic ferrets Mustela Putorius Furo in spain
Veterinary Parasitology, 2021Co-Authors: Sergio Villanuevasaz, Jacobo Giner, M M Alcover, Cristina Riera, Roser Fisa, M T Verde, Andres Yzuel, Hector Ruiz, D Lacasta, A FernandezAbstract:Abstract Zoonotic leishmaniosis due to Leishmania infantum is a vector-borne disease endemic in southern Europe and dogs are the main reservoir for this infection. Seasonal variations in antibody titers in this species in areas where phlebotomine vectors have seasonal patterns of activity are important for epidemiological, preventive and clinical studies related with canine leishmaniosis. It has been suggested that cats, rabbits and ferrets may act as peridomestic reservoirs and not only as accidental hosts. The aim of this study was to determine if seropositive ferrets (Mustela Putorius Furo) to Leishmania could be affected by seasonal variations of anti-Leishmania antibodies. A group of seropositive clinically healthy ferrets (n = 21) were included in this study. A significant reduction in anti-Leishmania infantum antibodies was detected during non-transmission period (December 2020-February 2021) in comparison to transmission period (April-October 2020). This study describes for the first time a seasonal variation in the anti-Leishmania antibodies detected in domestic ferrets following natural exposure during sand fly transmission period and the following non-sand fly transmission period in a Mediterranean area considered as an area where L. infantum is endemic.
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clinical leishmaniosis in a domestic ferret Mustela Putorius Furo treated with miltefosine plus allopurinol serological and clinical follow up
Veterinary Parasitology: Regional Studies and Reports, 2021Co-Authors: Jacobo Giner, M M Alcover, Cristina Riera, Roser Fisa, M T Verde, Sergio Villanuevasaz, A Fernandez, Andres YzuelAbstract:Abstract The published information on the treatment of mustelid leishmaniosis is extremely scarce because there are only two case reports available. In one case, a domestic ferret (Mustela Putorius Furo) was treated with a combination of meglumine antimoniate plus allopurinol and, in the other case, a therapeutic regimen with allopurinol was administrated to a Eurasian otter (Lutra lutra). This article describes for the first time a combined therapeutic protocol with miltefosine (2 mg/kg once a day during 28 days per os), and allopurinol (10 mg/kg twice a day PO sine die) in a domestic ferret with splenomegaly, lymphadenomegaly and a facial pyogranulomatous dermatitis, with a moderate level of antibodies to Leishmania infantum.
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sars cov 2 seroprevalence in household domestic ferrets Mustela Putorius Furo
Animal, 2021Co-Authors: Jacobo Giner, M T Verde, Andres Yzuel, Sergio Villanuevasaz, Ana Pilar Tobajas, M Perez, Ana Gonzalez, Ana Garciagarcia, Victor Taleb, Erandi LiranavarreteAbstract:Animal infections with SARS-CoV-2 have been reported in different countries and several animal species have been proven to be susceptible to infection with SARS-CoV-2 both naturally and by experimental infection. Moreover, infections under natural conditions in more than 20 mink farms have been reported where humans could have been the source of infection for minks. However, little information is available about the susceptibility of pet animals under natural conditions and currently there is no SARS-CoV-2 epidemiological assessment occurrence in household ferrets. In this study, the presence of SARS-CoV-2 antibodies was evaluated in serum samples obtained from 127 household ferrets (Mustela Putorius Furo) in the Province of Valencia (Spain). Two ferrets tested positive to SARS-CoV-2 (1.57%) by in-house enzyme-linked immunosorbent assay based on receptor binding domain (RBD) of Spike antigen. Furthermore, anti-RBD SARS-CoV-2 antibodies persisted at detectable levels in a seropositive SARS-CoV-2 domestic ferret beyond 129 days since the first time antibodies were detected. This study reports for the first time the evidence of household pet ferrets exposure to SARS-CoV-2 in Spain to date.
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first report on natural infection with leishmania infantum in a domestic ferret Mustela Putorius Furo in spain
Veterinary Parasitology: Regional Studies and Reports, 2020Co-Authors: Jacobo Giner, Asier Basurco, M M Alcover, Cristina Riera, Roser Fisa, R A Lopez, C Juansalles, M T Verde, Antonio Fernandez, Andres YzuelAbstract:A pet domestic ferret (Mustela Putorius Furo) with a papular lesion involving the right pinna was diagnosed with chronic pyogranulomatous dermatitis by histopathologic examination. Intralesional, intracytoplasmic oval microorganisms compatible with Leishmania spp. or Histoplasma spp. were observed in macrophages and multinucleate giant cells. Leishmania infantum (L. infantum) infection was diagnosed by PCR, culture in Novy-MacNeal-Nicolle medium, and immunohistochemistry. Abnormal clinicopathological results included increased alanine transferase, alkaline phosphatase, serum gamma glutamyl transferase and polyclonal gammpathy. Anti-Leishmania antibodies were detected by enzyme-linked immunosorbent assay, immunofluorescence antibody test and western blot using L. infantum antigen. Immunoreactivity against the 16 kDa specific L. infantum antigen fraction was observed by western blot. PCR performed in blood samples obtained from this patient after positive parasite isolation detected L. infantum DNA. To the authors' knowledge, this is the first diagnosis and isolation of L. infantum in a domestic ferret naturally infected in an endemic region (Spain) where canine and feline leishmaniosis is frequently detected. According to these findings, ferrets should be included as potential reservoir hosts of L. infantum. Future investigations should analyze the epidemiological role of ferrets in L. infantum infection including the prevalence of infection.
Sergio Villanuevasaz - One of the best experts on this subject based on the ideXlab platform.
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antibodies to leishmania in naturally exposed domestic ferrets Mustela Putorius Furo in spain
Veterinary Parasitology, 2021Co-Authors: Sergio Villanuevasaz, Jacobo Giner, M M Alcover, Cristina Riera, Roser Fisa, M T Verde, Andres Yzuel, Hector Ruiz, D Lacasta, A FernandezAbstract:Abstract Zoonotic leishmaniosis due to Leishmania infantum is a vector-borne disease endemic in southern Europe and dogs are the main reservoir for this infection. Seasonal variations in antibody titers in this species in areas where phlebotomine vectors have seasonal patterns of activity are important for epidemiological, preventive and clinical studies related with canine leishmaniosis. It has been suggested that cats, rabbits and ferrets may act as peridomestic reservoirs and not only as accidental hosts. The aim of this study was to determine if seropositive ferrets (Mustela Putorius Furo) to Leishmania could be affected by seasonal variations of anti-Leishmania antibodies. A group of seropositive clinically healthy ferrets (n = 21) were included in this study. A significant reduction in anti-Leishmania infantum antibodies was detected during non-transmission period (December 2020-February 2021) in comparison to transmission period (April-October 2020). This study describes for the first time a seasonal variation in the anti-Leishmania antibodies detected in domestic ferrets following natural exposure during sand fly transmission period and the following non-sand fly transmission period in a Mediterranean area considered as an area where L. infantum is endemic.
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clinical leishmaniosis in a domestic ferret Mustela Putorius Furo treated with miltefosine plus allopurinol serological and clinical follow up
Veterinary Parasitology: Regional Studies and Reports, 2021Co-Authors: Jacobo Giner, M M Alcover, Cristina Riera, Roser Fisa, M T Verde, Sergio Villanuevasaz, A Fernandez, Andres YzuelAbstract:Abstract The published information on the treatment of mustelid leishmaniosis is extremely scarce because there are only two case reports available. In one case, a domestic ferret (Mustela Putorius Furo) was treated with a combination of meglumine antimoniate plus allopurinol and, in the other case, a therapeutic regimen with allopurinol was administrated to a Eurasian otter (Lutra lutra). This article describes for the first time a combined therapeutic protocol with miltefosine (2 mg/kg once a day during 28 days per os), and allopurinol (10 mg/kg twice a day PO sine die) in a domestic ferret with splenomegaly, lymphadenomegaly and a facial pyogranulomatous dermatitis, with a moderate level of antibodies to Leishmania infantum.
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sars cov 2 seroprevalence in household domestic ferrets Mustela Putorius Furo
Animal, 2021Co-Authors: Jacobo Giner, M T Verde, Andres Yzuel, Sergio Villanuevasaz, Ana Pilar Tobajas, M Perez, Ana Gonzalez, Ana Garciagarcia, Victor Taleb, Erandi LiranavarreteAbstract:Animal infections with SARS-CoV-2 have been reported in different countries and several animal species have been proven to be susceptible to infection with SARS-CoV-2 both naturally and by experimental infection. Moreover, infections under natural conditions in more than 20 mink farms have been reported where humans could have been the source of infection for minks. However, little information is available about the susceptibility of pet animals under natural conditions and currently there is no SARS-CoV-2 epidemiological assessment occurrence in household ferrets. In this study, the presence of SARS-CoV-2 antibodies was evaluated in serum samples obtained from 127 household ferrets (Mustela Putorius Furo) in the Province of Valencia (Spain). Two ferrets tested positive to SARS-CoV-2 (1.57%) by in-house enzyme-linked immunosorbent assay based on receptor binding domain (RBD) of Spike antigen. Furthermore, anti-RBD SARS-CoV-2 antibodies persisted at detectable levels in a seropositive SARS-CoV-2 domestic ferret beyond 129 days since the first time antibodies were detected. This study reports for the first time the evidence of household pet ferrets exposure to SARS-CoV-2 in Spain to date.
