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I Irizar - One of the best experts on this subject based on the ideXlab platform.
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differentiating hair samples of the european mink Mustela lutreola the american mink Mustela vison and the european polecat Mustela putorius using light microscopy
Journal of Zoology, 2006Co-Authors: J Gonzalezesteban, I Villate, I IrizarAbstract:The European mink Mustela lutreola is a threatened species that is being monitored throughout its range, and innocuous methods of detection are needed as an alternative to live trapping, which may result in fatalities. A method is described for the identification of European mink hair by light microscopy, and its differentiation, on the basis of cuticular and medullary patterns of guard hairs, from hairs of similar mustelids, the introduced American mink Mustela vison and the European polecat Mustela putorius. When used in conjunction with hair trapping of living animals, this could prove to be the ideal way of conducting monitoring programmes on the European mink.
Kathie Burkett - One of the best experts on this subject based on the ideXlab platform.
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in vitro characterisation of sars cov 2 and susceptibility of domestic ferrets Mustela putorius furo
Transboundary and Emerging Diseases, 2021Co-Authors: Glenn A Marsh, Alexander J Mcauley, Sheree Brown, Elizabeth A Pharo, Sandra Crameri, Michelle L Baker, Jennifer A Barr, Jemma Bergfeld, Matthew P Bruce, Kathie BurkettAbstract:Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an emerging virus that has caused significant human morbidity and mortality since its detection in late 2019. With the rapid emergence has come an unprecedented programme of vaccine development with at least 300 candidates under development. Ferrets have proven to be an appropriate animal model for testing safety and efficacy of SARS-CoV-2 vaccines due to quantifiable virus shedding in nasal washes and oral swabs. Here, we outline our efforts early in the SARS-CoV-2 outbreak to propagate and characterize an Australian isolate of the virus in vitro and in an ex vivo model of human airway epithelium, as well as to demonstrate the susceptibility of domestic ferrets (Mustela putorius furo) to SARS-CoV-2 infection following intranasal challenge.
T Lode - One of the best experts on this subject based on the ideXlab platform.
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Genetic divergence without spatial isolation in polecat Mustela putorius populations
Journal of Evolutionary Biology, 2001Co-Authors: T LodeAbstract:Understanding how genetic divergence could exist without spatial isolation is a fundamental issue in biology. Although carnivores have previously been considered as having a weak genetic variability, polecats Mustela putorius from eight distinct populations exhibited both a strong polymorphism (17.5-22.5%) and a substantial allele effective number reaching Ne = 1.12. Heterozygosity ranging from Ho = 0.031-0.063 significantly differed among populations, while the mean FIS averaging 0.388 stressed a real deficiency of heterozygotes. Observed heterozygosity levels among populations did not correlate with any habitat types but were clearly associated with habitat diversity index. The habitat structure in polecat home range corresponded to habitat mosaic structure in which discrete habitat types alternated causing multifactorial constraints that may favour heterozygosity. Allozymic frequencies within populations did not vary with dominant habitat. But in the Tyrosinase-1, the rare homozygote BB, resulting in a 'dark' phenotype, was found much more in deciduous woods than the homozygote AA showing the 'typical' pattern. Thus, the genetic basis for a character differentiation was here evidenced in a remarkable situation without spatial isolation. Further, the very low proportion of heterozygotes for this locus suggests a disruptive effect and supports the prediction of intermediate phenotypes being at a disadvantage. This heterozygote deficit may also result from an assortative mating intra phenotype (homogamy). The divergence in polecat phenotypes showed that genetic differentiation can be induced by subtle variations in environment, a situation that is likely to be frequent in most natural populations, and emphasized the adaptive nature of habitat preference.
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Genetic heterozygosity in polecat Mustela putorius populations from western France.
Hereditas, 1998Co-Authors: T LodeAbstract:Allozymic variations were investigated in 49 European polecats Mustela putorius from Western France by starch gel electrophoresis. Out of 31 surveyed loci, eight (25.8%) were shown polymorphic and observed heterozygosity averaged 0.057. Deviations from Hardy-Weinberg equilibrium and heterozygote deficiency suggest that populations were not in panmixia. Heterozygotes for two loci or more totalled 42.9% of individuals. Thus, although carnivores were previously considered as less variable, polecat populations from Western France showed a high genetic variability.
J Gonzalezesteban - One of the best experts on this subject based on the ideXlab platform.
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differentiating hair samples of the european mink Mustela lutreola the american mink Mustela vison and the european polecat Mustela putorius using light microscopy
Journal of Zoology, 2006Co-Authors: J Gonzalezesteban, I Villate, I IrizarAbstract:The European mink Mustela lutreola is a threatened species that is being monitored throughout its range, and innocuous methods of detection are needed as an alternative to live trapping, which may result in fatalities. A method is described for the identification of European mink hair by light microscopy, and its differentiation, on the basis of cuticular and medullary patterns of guard hairs, from hairs of similar mustelids, the introduced American mink Mustela vison and the European polecat Mustela putorius. When used in conjunction with hair trapping of living animals, this could prove to be the ideal way of conducting monitoring programmes on the European mink.
Huw I Griffiths - One of the best experts on this subject based on the ideXlab platform.
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mitochondrial phylogeography and population history of pine martens martes martes compared with polecats Mustela putorius
Molecular Ecology, 2002Co-Authors: Angus Davison, Johnny D S Birks, Rachael C Brookes, John Messenger, Huw I GriffithsAbstract:The flora and fauna of Europe are linked by a common biogeographic history, most recently the Pleistocene glaciations that restricted the range of most species to southern refugial populations. Changes in population size and migration, as well as selection, have all left a signature on the genetic differentiation. Thus, three paradigms of postglacial recolonization have been described, inferred from the patterns of DNA differentiation. Yet some species, especially wide-ranging carnivores, exhibit little population structuring between the proposed refugia, although relatively few have been studied due to the difficulty of obtaining samples. Therefore, we investigated mitochondrial variation in pine martens, Martes martes, in order to understand the extent to which they were affected by glacial cycles, and compared the results with an analysis of sequences from polecats, Mustela putorius. A general lack of ancient lineages, and a mismatch distribution that is consistent with an expanding population, is evidence that the present-day M. martes and Mu. putorius in central and northern Europe colonized from a single European refugium following a recent glaciation. There has also been interspecific mitochondrial introgression between M. martes and the sable M. zibellina in Fennoscandia.
