Starch Gel Electrophoresis

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L Pinteric - One of the best experts on this subject based on the ideXlab platform.

  • quantitation of Starch Gel Electrophoresis
    Annals of the New York Academy of Sciences, 2006
    Co-Authors: James H Pert, L Pinteric
    Abstract:

    Summary Quantitative high-voltage Starch Gel Electrophoresis has been found to be useful as an analytical tool in the study of purified proteins. Much new information can be obtained about complex solutions, such as human plasma, with this method. One of the objectives has been to develop a procedure for high-speed scanning of large numbers of samples so that the results could be programmed for a digital computer and statistical evaluations could be made. The difficulties of achieving this objective have been discussed, and a new approach to instrumentation is presented that is designed to minimize these problems.

James H Pert - One of the best experts on this subject based on the ideXlab platform.

  • quantitation of Starch Gel Electrophoresis
    Annals of the New York Academy of Sciences, 2006
    Co-Authors: James H Pert, L Pinteric
    Abstract:

    Summary Quantitative high-voltage Starch Gel Electrophoresis has been found to be useful as an analytical tool in the study of purified proteins. Much new information can be obtained about complex solutions, such as human plasma, with this method. One of the objectives has been to develop a procedure for high-speed scanning of large numbers of samples so that the results could be programmed for a digital computer and statistical evaluations could be made. The difficulties of achieving this objective have been discussed, and a new approach to instrumentation is presented that is designed to minimize these problems.

Kathleen M Cole - One of the best experts on this subject based on the ideXlab platform.

  • an evaluation of species relationships in the porphyra perforata complex bangiales rhodophyta using Starch Gel Electrophoresis
    Hydrobiologia, 1990
    Co-Authors: Sandra C Lindstrom, Kathleen M Cole
    Abstract:

    Traditional morphological features have formed the basis for distinguishing species of Porphyra. Among these features are number of cell layers, number of chloroplasts per cell, arrangement of reproductive structures on the thallus, and overall morphology. Chromosome number and chromosome morphology have helped corroborate some species identities. A survey of northeast Pacific species of Porphyra using Starch Gel Electrophoresis of 15 soluble proteins has shown that electrophoretic banding patterns provide a reliable diagnostic tool for species identification. Data from Starch Gel Electrophoresis are presented to confirm the identities of species formerly associated with the Porphyra perforata species-complex in British Columbia and northern Washington. Porphyra abbottae, P. fallax, P. kanakaensis, and P. torta are recognized as distinct species, and Porphyra sanjuanensis is synonymized with P. perforata.

Sandra C Lindstrom - One of the best experts on this subject based on the ideXlab platform.

  • an evaluation of species relationships in the porphyra perforata complex bangiales rhodophyta using Starch Gel Electrophoresis
    Hydrobiologia, 1990
    Co-Authors: Sandra C Lindstrom, Kathleen M Cole
    Abstract:

    Traditional morphological features have formed the basis for distinguishing species of Porphyra. Among these features are number of cell layers, number of chloroplasts per cell, arrangement of reproductive structures on the thallus, and overall morphology. Chromosome number and chromosome morphology have helped corroborate some species identities. A survey of northeast Pacific species of Porphyra using Starch Gel Electrophoresis of 15 soluble proteins has shown that electrophoretic banding patterns provide a reliable diagnostic tool for species identification. Data from Starch Gel Electrophoresis are presented to confirm the identities of species formerly associated with the Porphyra perforata species-complex in British Columbia and northern Washington. Porphyra abbottae, P. fallax, P. kanakaensis, and P. torta are recognized as distinct species, and Porphyra sanjuanensis is synonymized with P. perforata.

Henry Hong - One of the best experts on this subject based on the ideXlab platform.

  • allozyme comparison of three trypanosoma species kinetoplastida trypanosomatidae of toads and frogs by Starch Gel Electrophoresis
    Journal of Parasitology, 1992
    Co-Authors: Donald S Martin, Sherwin S Desser, Henry Hong
    Abstract:

    : Six metabolic enzymes, glucose-6-phosphate dehydrogenase, glucosephosphate isomerase, isocitrate dehydrogenase, malate dehydrogenase, phosphoglucomutase, and purine nucleoside phosphorylase, from clonal isolates of 3 presumptive species of Trypanosoma (T. fallisi, T. ranarum, and T. rotatorium) from 3 anuran hosts (Bufo americanus, Rana clamitans, and Rana catesbeiana) were compared using Starch-Gel Electrophoresis. Although bands were shared among the different zymodemes of isolates of the same host genus, low genetic polymorphism of the enzyme loci was observed with few apparent shared bands between samples isolated from frogs and toads. A distance value calculated between toad and frog trypanosome isolates suggests the likelihood of long-time separation of species. Cluster analysis based on overall similarity distinguished the trypanosomes of toads and frogs as separate taxa, suggesting that host specificity and observed morphological differences are consistent with heritable allozyme differences.