The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform
Rashmi Sinha - One of the best experts on this subject based on the ideXlab platform.
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meat related Mutagens and pancreatic cancer null results from a clinic based case control study
Cancer Epidemiology Biomarkers & Prevention, 2013Co-Authors: Rick J Janse, Dennis P Robinso, Rya D Frank, Rachael Z Stolzenbergsolomo, William R Amle, A L Oberg, Kari G Rabe, Jane E Olso, Gloria M Peterse, Rashmi SinhaAbstract:Background: Pancreatic cancer is a devastating disease for which the role of dietary factors remains inconclusive. The study objective was to evaluate risk of pancreatic cancer associated with meat preparation methods and meat-related mutagen consumption using a clinic-based case–control design. Methods: There were 384 cases and 983 controls; subjects provided demographic information and completed a 144-item food frequency questionnaire, which was used to estimate meat mutagen intake using the National Cancer Institute's CHARRED database (Bethesda, MD). Logistic regression was used to calculate ORs and 95% confidence intervals (CI), adjusted for factors including age, sex, cigarette smoking, body mass index, and diabetes mellitus. Results: Overall, the findings were null with respect to meat mutagen intake and pancreatic cancer. Conclusions: The results do not support an association between well-done meat or meat-related mutagen intake and pancreatic cancer and contrast with generally increased risks reported in previous studies. Impact: These data contribute to evidence about pancreatic cancer and potentially carcinogenic compounds in meat. Cancer Epidemiol Biomarkers Prev; 00(00); 1–. ©2013 AACR .
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no effect of meat meat cooking preferences meat Mutagens or heme iron on lung cancer risk in the prostate lung colorectal and ovarian cancer screening trial
International Journal of Cancer, 2011Co-Authors: Natasa Tasevska, Amanda J Cross, Neil E Caporaso, Regina G Ziegle, Kevi W Dodd, Rashmi SinhaAbstract:Recent epidemiological studies have suggested that red and processed meat may increase the risk of lung cancer. Possible underlying mechanisms include Mutagens produced during high-temperature cooking or preservation, or formed endogenously from heme iron in meat. We used data from 99,579 participants of both screened and nonscreened arms of the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial, aged 55–74 years, to investigate whether meat type, cooking method, doneness level, intake of specific meat Mutagens 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline] (DiMeIQx), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and benzo(a)pyrene (B(a)P)] and heme iron are associated with lung cancer. Participants' diet was assessed prospectively using a 124-item food frequency questionnaire and an additional meat-cooking module. Dietary data were used in conjunction with a database to estimate intake of MeIQx, DiMeIQx, PhIP, B(a)P and heme iron. After up to 8 years of follow-up, 782 incident lung cancer cases were ascertained. Lung cancer risk was not associated with the consumption of either red (men: HRQ5 vs. Q1 = 1.11, 95% CI = 0.79–1.56, Ptrend = 0.42; women: HRQ5 vs. Q1 = 1.30, 95% CI = 0.87–1.95, Ptrend = 0.65) or processed meat (men: HRQ5 vs. Q1 = 1.12, 95% CI = 0.83–1.53, Ptrend = 0.22; women: HRQ5 vs. Q1 = 0.98, 95% CI = 0.68–1.41, Ptrend = 0.32) in multivariable models. High-temperature cooking methods, level of meat doneness, meat Mutagens and heme iron had no effect on lung cancer risk. In this population, we found no association between meat type, cooking method, doneness level or intake of specific meat Mutagens or heme iron and lung cancer risk.
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intake of meat meat Mutagens and iron and the risk of breast cancer in the prostate lung colorectal and ovarian cancer screening trial
British Journal of Cancer, 2009Co-Authors: Amanda J Cross, L Ferrucci, Arry I Graubard, Louise A Into, Catherine A Mccarty, Regina G Ziegle, Susa T Mayne, Rashmi SinhaAbstract:Intake of meat, meat Mutagens, and iron and the risk of breast cancer in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial
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intakes of red meat processed meat and meat Mutagens increase lung cancer risk
Cancer Research, 2009Co-Authors: Tram Kim Lam, Amanda J Cross, Dario Consonni, G Randi, Vincenzo Agnardi, Pier Alberto Ertazzi, Neil E Caporaso, Rashmi Sinha, Amy F Suba, Maria Teresa LandiAbstract:Red and processed meat intake may increase lung cancer risk. However, the epidemiologic evidence is inconsistent and few studies have evaluated the role of meat Mutagens formed during high cooking temperatures. We investigated the association of red meat, processed meat, and meat mutagen intake with lung cancer risk in Environment And Genetics in Lung cancer Etiology, a population-based case-control study. Primary lung cancer cases ( n = 2,101) were recruited from 13 hospitals within the Lombardy region of Italy examining ∼80% of the cases from the area. Noncancer population controls ( n = 2,120), matched to cases on gender, residence, and age, were randomly selected from the same catchment area. Diet was assessed in 1,903 cases and 2,073 controls and used in conjunction with a meat mutagen database to estimate intake of heterocyclic amines (HCA) and benzo( a )pyrene (BaP). Multivariable odds ratios (OR) and 95% confidence intervals (95% CI) for sex-specific tertiles of intake were calculated using unconditional logistic regression. Red and processed meat were positively associated with lung cancer risk (highest-versus-lowest tertile: OR, 1.8; 95% CI, 1.5–2.2; P trend P trend P trend = 0.001 and OR, 2.5; 95% CI, 1.5–4.2; P trend = 0.001, respectively). HCAs and BaP were significantly associated with increased risk of lung cancer. When separated by histology, significant positive associations for both meat groups were restricted to adenocarcinoma and squamous cell carcinoma but not small cell carcinoma of the lung. In summary, red meat, processed meat, and meat Mutagens were independently associated with increased risk of lung cancer. [Cancer Res 2009;69(3):932–9]
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meat intake preparation methods Mutagens and colorectal adenoma recurrence
Carcinogenesis, 2007Co-Authors: Maria Elena Martinez, Rashmi Sinha, Elizabeth T Jacobs, Eri L Ashbeck, Pete Lance, David S Alberts, Patricia A ThompsoAbstract:Red meat intake has been shown to be associated with higher risk of colorectal cancer. Though the exact mechanisms responsible for this association remain unknown, several tumorigenic properties of meat have been proposed. One well-supported biologic mechanism is elevated exposure to the genotoxic formation of heterocyclic amines (HCAs), which occur when meat is cooked at high temperatures for a long period of time. We prospectively assessed the relation between type of meat, meat preparation method, doneness, a metric of HCAs and other Mutagens and colorectal adenoma recurrence among 869 participants in a chemoprevention trial of ursodeoxycholic acid. Unconditional logistic regression analyses were used to estimate odds ratios (ORs) and associated 95% confidence intervals (CIs). Most meat variables assessed were positively but weakly associated with recurrence of any adenoma. In contrast, recurrence of advanced or multiple adenomas was more strongly associated with a number of the meat exposure variables evaluated. For recurrence of advanced lesions, significant associations were detected among individuals in the highest when compared with the lowest tertile of intake for pan-fried red meat (OR = 1.85; 95% CI = 1.10-3.13) and well/very well done red meat (OR = 1.71; 95% CI = 1.02-2.86). Significant positive associations were shown for recurrence of multiple adenomas and the following variables: processed meat (OR = 1.83; 95% CI = 1.10-3.04), pan-fried red meat (OR = 1.63; 95% CI = 1.01-2.61), well/very well done red meat (OR = 1.68; 95% CI = 1.03-2.74), 2-amino-3,4,8-trimethylimidazo[4,5,-f]quinoxaline (OR = 1.74; 95% CI = 1.07-2.82) and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (OR = 1.68; 95% CI = 1.03-2.75). Our results support a meat mutagen exposure hypothesis as a potential mechanism for recurrence of clinically significant adenomatous polyps.
Yu F Sasaki - One of the best experts on this subject based on the ideXlab platform.
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detection of dna lesions induced by chemical Mutagens using the single cell gel electrophoresis comet assay 2 relationship between dna migration and alkaline condition
Mutation Research, 1997Co-Authors: Youichi Miyamae, Shuji Tsuda, Kouichi Iwasaki, Naohide Kinae, Michiko Murakami, Makiko Tanaka, Yu F SasakiAbstract:The alkaline condition is an important factor for the alkaline single-cell gel electrophoresis (SCG) assay to detect the genotoxic effects of chemicals. In order to understand the relationship between DNA migration and alkaline condition, the effect of 13 model chemical Mutagens with different modes of action was evaluated with the alkaline SCG assay under two different alkaline conditions (pH 12.1 and 12.6). CHO cells were sampled just after treatment for 1 h. The X-ray mimetic mutagen BLM increased DNA migration at pH 12.1 and 12.6 and the results were the same at both pH values. Six alkylating Mutagens MNU, ENU, MNNG, ENNG, MMS, and EMS and one base adduct inducer 4-NQO induced a dose-dependent response only at pH 12.6. Two DNA crosslinking agents, MMC and DDP, and AMD had negative results. MMC and DDP, however, reduced the positive response of BLM, suggesting that DNA crosslinks could be detected. These results demonstrated that the alkaline condition was important factor for the alkaline SCG assay to detect the genotoxic effects of chemicals.
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simple detection of chemical Mutagens by the alkaline single cell gel electrophoresis comet assay in multiple mouse organs liver lung spleen kidney and bone marrow
Mutation Research-genetic Toxicology and Environmental Mutagenesis, 1997Co-Authors: Yu F Sasaki, Fusako Izumiyama, Emi Nishidate, Naonori Matsusaka, Shuji TsudaAbstract:Recently, we designed a fast and simple method to obtain nuclei for the alkaline SCG assay and we tested it with mouse liver, lung, kidney, spleen, and bone marrow. Instead of isolating organ cells by trypsinization, we homogenized tissue and isolated the nuclei. Each organ was minced, and the mince was suspended in chilled homogenizing buffer containing NaCl and Na2EDTA, homogenized gently using a Potter-type homogenizer set in ice, and then centrifuged. The nuclei from the precipitate were used for the assay. To evaluate the validity of this method, we tested the genotoxicity in mouse organs of 11 chemical Mutagens with different modes of action. Mice were sacrificed 3 and 24 h after administration of each mutagen. Treatment with three alkylating agents (MMS, EMS, and MNNG), a DNA crosslinking agent (MMC), two aromatic amines (2-AAF and phenacetin), a polycyclic aromatic hydrocarbon (B[a]P), and two inorganic chemicals (KBrO3 and K2CrO4) increased migration of the DNA from mouse organs. 5-FU (a base analog) and colchicine (a spindle poison) treatment produced negative results in all organ studied. Considering that the alkaline SCG assay detects genotoxicity as DNA fragments derived from DNA single-strand breaks and alkali-labile damage, our results showed that the SCG assay using our homogenization technique detected chemical Mutagens as a function of their modes of action.
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detection of chemically induced dna lesions in multiple mouse organs liver lung spleen kidney and bone marrow using the alkaline single cell gel electrophoresis comet assay
Mutation Research-genetic Toxicology and Environmental Mutagenesis, 1997Co-Authors: Yu F Sasaki, Shuji Tsuda, Fusako Izumiyama, Emi NishidateAbstract:Abstract The effect of 2 model chemical Mutagens on DNA was evaluated with the alkaline single cell gel electrophoresis (SCG) (Comet) assay in 5 mouse organs – liver, lung, kidney, spleen and bone marrow. Mice were sacrificed 3 and 24 h after the administration of the direct mutagen ethyl nitrosourea (ENU) or the liver-targeting promutagen p -dimethylaminoazobenzene (DAB). Each organ was minced, suspended at a concentration of 1 g/ml in chilled homogenizing buffer (pH 7.5) containing 0.075 M NaCl and 0.024 M Na 2 EDTA, homogenized gently using a Potter-type homogenizer at 500–800 rpm set in ice, and then centrifuged nuclei were used for the alkaline SCG assay. ENU induced DNA damage in cells all of the organs studied. DAB, on the other hand, produced a positive response in the liver only. We suggest that it may be possible to use the alkaline SCG assay using a homogenization technique to detect the genotoxicity of chemicals in vivo in their target organs.
Amanda J Cross - One of the best experts on this subject based on the ideXlab platform.
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no effect of meat meat cooking preferences meat Mutagens or heme iron on lung cancer risk in the prostate lung colorectal and ovarian cancer screening trial
International Journal of Cancer, 2011Co-Authors: Natasa Tasevska, Amanda J Cross, Neil E Caporaso, Regina G Ziegle, Kevi W Dodd, Rashmi SinhaAbstract:Recent epidemiological studies have suggested that red and processed meat may increase the risk of lung cancer. Possible underlying mechanisms include Mutagens produced during high-temperature cooking or preservation, or formed endogenously from heme iron in meat. We used data from 99,579 participants of both screened and nonscreened arms of the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial, aged 55–74 years, to investigate whether meat type, cooking method, doneness level, intake of specific meat Mutagens 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline] (DiMeIQx), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and benzo(a)pyrene (B(a)P)] and heme iron are associated with lung cancer. Participants' diet was assessed prospectively using a 124-item food frequency questionnaire and an additional meat-cooking module. Dietary data were used in conjunction with a database to estimate intake of MeIQx, DiMeIQx, PhIP, B(a)P and heme iron. After up to 8 years of follow-up, 782 incident lung cancer cases were ascertained. Lung cancer risk was not associated with the consumption of either red (men: HRQ5 vs. Q1 = 1.11, 95% CI = 0.79–1.56, Ptrend = 0.42; women: HRQ5 vs. Q1 = 1.30, 95% CI = 0.87–1.95, Ptrend = 0.65) or processed meat (men: HRQ5 vs. Q1 = 1.12, 95% CI = 0.83–1.53, Ptrend = 0.22; women: HRQ5 vs. Q1 = 0.98, 95% CI = 0.68–1.41, Ptrend = 0.32) in multivariable models. High-temperature cooking methods, level of meat doneness, meat Mutagens and heme iron had no effect on lung cancer risk. In this population, we found no association between meat type, cooking method, doneness level or intake of specific meat Mutagens or heme iron and lung cancer risk.
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intake of meat meat Mutagens and iron and the risk of breast cancer in the prostate lung colorectal and ovarian cancer screening trial
British Journal of Cancer, 2009Co-Authors: Amanda J Cross, L Ferrucci, Arry I Graubard, Louise A Into, Catherine A Mccarty, Regina G Ziegle, Susa T Mayne, Rashmi SinhaAbstract:Intake of meat, meat Mutagens, and iron and the risk of breast cancer in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial
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intakes of red meat processed meat and meat Mutagens increase lung cancer risk
Cancer Research, 2009Co-Authors: Tram Kim Lam, Amanda J Cross, Dario Consonni, G Randi, Vincenzo Agnardi, Pier Alberto Ertazzi, Neil E Caporaso, Rashmi Sinha, Amy F Suba, Maria Teresa LandiAbstract:Red and processed meat intake may increase lung cancer risk. However, the epidemiologic evidence is inconsistent and few studies have evaluated the role of meat Mutagens formed during high cooking temperatures. We investigated the association of red meat, processed meat, and meat mutagen intake with lung cancer risk in Environment And Genetics in Lung cancer Etiology, a population-based case-control study. Primary lung cancer cases ( n = 2,101) were recruited from 13 hospitals within the Lombardy region of Italy examining ∼80% of the cases from the area. Noncancer population controls ( n = 2,120), matched to cases on gender, residence, and age, were randomly selected from the same catchment area. Diet was assessed in 1,903 cases and 2,073 controls and used in conjunction with a meat mutagen database to estimate intake of heterocyclic amines (HCA) and benzo( a )pyrene (BaP). Multivariable odds ratios (OR) and 95% confidence intervals (95% CI) for sex-specific tertiles of intake were calculated using unconditional logistic regression. Red and processed meat were positively associated with lung cancer risk (highest-versus-lowest tertile: OR, 1.8; 95% CI, 1.5–2.2; P trend P trend P trend = 0.001 and OR, 2.5; 95% CI, 1.5–4.2; P trend = 0.001, respectively). HCAs and BaP were significantly associated with increased risk of lung cancer. When separated by histology, significant positive associations for both meat groups were restricted to adenocarcinoma and squamous cell carcinoma but not small cell carcinoma of the lung. In summary, red meat, processed meat, and meat Mutagens were independently associated with increased risk of lung cancer. [Cancer Res 2009;69(3):932–9]
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meat related Mutagens carcinogens in the etiology of colorectal cancer
Environmental and Molecular Mutagenesis, 2004Co-Authors: Amanda J Cross, Rashmi SinhaAbstract:Diets containing substantial amounts of red or preserved meats may increase the risk of various cancers, including colorectal cancer. This association may be due to a combination of factors such as the content of fat, protein, iron, and/or meat preparation (e.g., cooking or preserving methods). Red meat may be associated with colorectal cancer by contributing to N-nitroso compound (NOC) exposure. Humans can be exposed to NOCs by exogenous routes (from processed meats in particular) and by endogenous routes. Endogenous exposure to NOCs is dose-dependently related to the amount of red meat in the diet. Laboratory results have shown that meats cooked at high temperatures contain other potential Mutagens in the form of heterocyclic amines (HCAs) and polycyclic aromatic hydrocarbons (PAHs). To investigate the role of these compounds, we have created separate databases for HCAs and PAHs, which we have used in conjunction with a validated meat-cooking food frequency questionnaire. The role of meat type, cooking methods, doneness levels, and meat-cooking Mutagens has been examined in both case-control studies and prospective cohort studies, with mixed results. Here, we review the current epidemiologic knowledge of meat-related Mutagens, and evaluate the types of studies that may be required in the future to clarify the association between meat consumption and colorectal cancer.
Shuji Tsuda - One of the best experts on this subject based on the ideXlab platform.
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detection of dna lesions induced by chemical Mutagens using the single cell gel electrophoresis comet assay 2 relationship between dna migration and alkaline condition
Mutation Research, 1997Co-Authors: Youichi Miyamae, Shuji Tsuda, Kouichi Iwasaki, Naohide Kinae, Michiko Murakami, Makiko Tanaka, Yu F SasakiAbstract:The alkaline condition is an important factor for the alkaline single-cell gel electrophoresis (SCG) assay to detect the genotoxic effects of chemicals. In order to understand the relationship between DNA migration and alkaline condition, the effect of 13 model chemical Mutagens with different modes of action was evaluated with the alkaline SCG assay under two different alkaline conditions (pH 12.1 and 12.6). CHO cells were sampled just after treatment for 1 h. The X-ray mimetic mutagen BLM increased DNA migration at pH 12.1 and 12.6 and the results were the same at both pH values. Six alkylating Mutagens MNU, ENU, MNNG, ENNG, MMS, and EMS and one base adduct inducer 4-NQO induced a dose-dependent response only at pH 12.6. Two DNA crosslinking agents, MMC and DDP, and AMD had negative results. MMC and DDP, however, reduced the positive response of BLM, suggesting that DNA crosslinks could be detected. These results demonstrated that the alkaline condition was important factor for the alkaline SCG assay to detect the genotoxic effects of chemicals.
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simple detection of chemical Mutagens by the alkaline single cell gel electrophoresis comet assay in multiple mouse organs liver lung spleen kidney and bone marrow
Mutation Research-genetic Toxicology and Environmental Mutagenesis, 1997Co-Authors: Yu F Sasaki, Fusako Izumiyama, Emi Nishidate, Naonori Matsusaka, Shuji TsudaAbstract:Recently, we designed a fast and simple method to obtain nuclei for the alkaline SCG assay and we tested it with mouse liver, lung, kidney, spleen, and bone marrow. Instead of isolating organ cells by trypsinization, we homogenized tissue and isolated the nuclei. Each organ was minced, and the mince was suspended in chilled homogenizing buffer containing NaCl and Na2EDTA, homogenized gently using a Potter-type homogenizer set in ice, and then centrifuged. The nuclei from the precipitate were used for the assay. To evaluate the validity of this method, we tested the genotoxicity in mouse organs of 11 chemical Mutagens with different modes of action. Mice were sacrificed 3 and 24 h after administration of each mutagen. Treatment with three alkylating agents (MMS, EMS, and MNNG), a DNA crosslinking agent (MMC), two aromatic amines (2-AAF and phenacetin), a polycyclic aromatic hydrocarbon (B[a]P), and two inorganic chemicals (KBrO3 and K2CrO4) increased migration of the DNA from mouse organs. 5-FU (a base analog) and colchicine (a spindle poison) treatment produced negative results in all organ studied. Considering that the alkaline SCG assay detects genotoxicity as DNA fragments derived from DNA single-strand breaks and alkali-labile damage, our results showed that the SCG assay using our homogenization technique detected chemical Mutagens as a function of their modes of action.
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detection of chemically induced dna lesions in multiple mouse organs liver lung spleen kidney and bone marrow using the alkaline single cell gel electrophoresis comet assay
Mutation Research-genetic Toxicology and Environmental Mutagenesis, 1997Co-Authors: Yu F Sasaki, Shuji Tsuda, Fusako Izumiyama, Emi NishidateAbstract:Abstract The effect of 2 model chemical Mutagens on DNA was evaluated with the alkaline single cell gel electrophoresis (SCG) (Comet) assay in 5 mouse organs – liver, lung, kidney, spleen and bone marrow. Mice were sacrificed 3 and 24 h after the administration of the direct mutagen ethyl nitrosourea (ENU) or the liver-targeting promutagen p -dimethylaminoazobenzene (DAB). Each organ was minced, suspended at a concentration of 1 g/ml in chilled homogenizing buffer (pH 7.5) containing 0.075 M NaCl and 0.024 M Na 2 EDTA, homogenized gently using a Potter-type homogenizer at 500–800 rpm set in ice, and then centrifuged nuclei were used for the alkaline SCG assay. ENU induced DNA damage in cells all of the organs studied. DAB, on the other hand, produced a positive response in the liver only. We suggest that it may be possible to use the alkaline SCG assay using a homogenization technique to detect the genotoxicity of chemicals in vivo in their target organs.
L Ferrucci - One of the best experts on this subject based on the ideXlab platform.
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intake of meat meat Mutagens and iron and the risk of breast cancer in the prostate lung colorectal and ovarian cancer screening trial
British Journal of Cancer, 2009Co-Authors: Amanda J Cross, L Ferrucci, Arry I Graubard, Louise A Into, Catherine A Mccarty, Regina G Ziegle, Susa T Mayne, Rashmi SinhaAbstract:Intake of meat, meat Mutagens, and iron and the risk of breast cancer in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial
