The Experts below are selected from a list of 29214 Experts worldwide ranked by ideXlab platform
Caihong Dong - One of the best experts on this subject based on the ideXlab platform.
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nutritional requirements for Mycelial Growth of milk white toothed mushroom irpex lacteus agaricomycetes in submerged culture
International Journal of Medicinal Mushrooms, 2017Co-Authors: Xiaoming Dong, Xinhua Song, Caihong DongAbstract:Irpex lacteus, a medicinal fungus, is used in traditional Chinese medicine to treat chronic glomerulonephritis. In this work, a strain of I. lacteus was isolated from the fruiting body of a wild specimen and identified by ITS-5.8S ribosomal DNA sequencing analysis. Then the nutritional requirements and culture conditions for Mycelial Growth of I. lacteus in semisynthetic liquid media were investigated using the one-factor-at-a-time and orthogonal matrix methods. Optimum Growth occurred at 30°C and 35°C. I. lacteus mycelia grew well at pH values between 3 and 9, suggesting that this strain is not sensitive to pH. The nutritional components, including 9 carbohydrates, 9 nitrogen compounds, 11 vitamins, and 10 mineral elements, were studied for their effects on Mycelial Growth in submerged cultures of I. lacteus. Among these variables, soluble starch, peptone, yeast extract, and calcium chloride were identified as required for optimum Mycelial Growth. The concentrations of each component were optimized using an orthogonal design, and the effects of medium composition on Mycelial Growth were found in the order soluble starch > yeast extract > peptone > calcium chloride. The optimal concentrations of these components for Mycelial Growth were determined to be 60 g/L soluble starch, 35 g/L peptone, 15 g/L yeast extract, and 0.6 g/L calcium chloride. Under the optimum medium and culture conditions, the maximum biomass reached 13.73 g/L after 3 days in submerged culture, a value over twice that reached using the basal medium. These results provide a basis for further physiological study and industrial fermentation of I. lacteus.
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nutritional requirements of Mycelial Growth of cordyceps sinensis in submerged culture
Journal of Applied Microbiology, 2005Co-Authors: Caihong DongAbstract:C.-H. DONG AND Y.-J. YAO. 2005. Aims: The nutritional requirements for Mycelial Growth of Cordyceps sinensis in semi-synthetic liquid media were investigated. The results provide a basis for further physiological study and industrial fermentation of the fungus. Methods and Results: Nutritional requirements, including 17 carbohydrates, 16 nitrogen compounds, nine vitamins, four macro-elements, four trace-elements and eight ratios of carbon to nitrogen, were studied for their effects on the Mycelial Growth in submerged cultures of C. sinensis by using one-factor-at-a-time and orthogonal matrix methods. Among these variables, sucrose, peptone, folic acid, calcium, zinc and a carbon to nitrogen ratio 12 : 1 were identified as the requirements for the optimum Mycelial Growth. The concentrations of sucrose, peptone and yeast extract were optimized and the effects of medium composition on Mycelial Growth were found to be in the order sucrose > yeast extract > peptone. The optimal concentration for Mycelial Growth was determined as 50 g l )1 sucrose, 10 g l )1 peptone and 3 g l )1 yeast extract. Conclusions: Under optimal culture conditions, over 22 g l )1 of Mycelial biomass could be obtained after 40 days in submerged cultures. Significance and Impact of the Study: Cordyceps sinensis, one of the most valued medicinal fungi, is shown to grow in axenic culture. This is the first report on nutritional requirements and design of a simplified semisynthetic medium for Mycelial Growth of this psychrophilic species, which grows slowly below 20� C. The results of this study will facilitate research on mass production of the fungus under defined culture conditions.
Ynfuu Ke - One of the best experts on this subject based on the ideXlab platform.
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effect of fatty acids on the Mycelial Growth and polysaccharide formation by ganoderma lucidum in shake flask cultures
Enzyme and Microbial Technology, 2000Co-Authors: Fanchiang Yang, Ynfuu KeAbstract:Fatty acids were added into the media to investigate their effects on the Mycelial Growth and polysaccharide formation by Ganoderma lucidum. The experiments were carried out in freely suspended cultures or immobilized cultures using shake flasks. The results indicate that the extent of stimulation or inhibition were associated with the types and levels of fatty acids. Oleic acid at the level of 0.15 g/100 ml led to a significant increase in cell concentration from 0.20 to 0.46 g/100 ml in a suspended culture and palmitic acid was of great advantage to polysaccharide production. In contrast, linoleic acid (0.1 g/100 ml) drastically suppressed both Mycelial Growth and polysaccharide formation. In immobilized cultures with fatty acids, the stimulation of Mycelial Growth remained the same level, but the enhancement of polysaccharide production became less. In addition, the Growth of G. lucidum in the pattern of immobilization might be beneficial to the production of mycelia and polysaccharide.
Fanchiang Yang - One of the best experts on this subject based on the ideXlab platform.
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effect of fatty acids on the Mycelial Growth and polysaccharide formation by ganoderma lucidum in shake flask cultures
Enzyme and Microbial Technology, 2000Co-Authors: Fanchiang Yang, Ynfuu KeAbstract:Fatty acids were added into the media to investigate their effects on the Mycelial Growth and polysaccharide formation by Ganoderma lucidum. The experiments were carried out in freely suspended cultures or immobilized cultures using shake flasks. The results indicate that the extent of stimulation or inhibition were associated with the types and levels of fatty acids. Oleic acid at the level of 0.15 g/100 ml led to a significant increase in cell concentration from 0.20 to 0.46 g/100 ml in a suspended culture and palmitic acid was of great advantage to polysaccharide production. In contrast, linoleic acid (0.1 g/100 ml) drastically suppressed both Mycelial Growth and polysaccharide formation. In immobilized cultures with fatty acids, the stimulation of Mycelial Growth remained the same level, but the enhancement of polysaccharide production became less. In addition, the Growth of G. lucidum in the pattern of immobilization might be beneficial to the production of mycelia and polysaccharide.
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effects of cultivating conditions on the Mycelial Growth of ganoderma lucidum in submerged flask cultures
Bioprocess Engineering, 1998Co-Authors: Fanchiang Yang, C B LiauAbstract:In this paper the effects of environmental conditions on the Mycelial Growth of Ganoderma lucidum were investigated in shake flask cultures. The optimal temperature and pH were found to be around 30–35 °C and 4, respectively, in a glucose-ammonium chloride medium. The maximum Mycelial concentration reached to around 350 mg/100 ml. The formation of Mycelial pellets and their ultra structure was demonstrated to be greatly affected by cultivating conditions. Increasing surface aeration would be beneficial for Mycelial Growth. However, too high rotating speed in shake cultures had a detrimental effect on the formation of Mycelial pellets and the optimum was found to be 100 rpm.
Z Q Liang - One of the best experts on this subject based on the ideXlab platform.
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optimization of submerged culture requirements for the production of Mycelial Growth and exopolysaccharide by cordyceps jiangxiensis jxpj 0109
Journal of Applied Microbiology, 2004Co-Authors: Jianhui Xiao, Daixiong Chen, Ning Fang, Yu Xiao, Y Qi, Z Q LiangAbstract:Aims: The objective of the present study was to investigate the optimal culture requirements for Mycelial Growth and exopolysaccharide production by Cordyceps jiangxiensis JXPJ 0109 in submerged culture. Methods and Results: The effects of medium ingredients (i.e. carbon and nitrogen sources, and Growth factor) and other culture requirements (i.e. initial pH, temperature, etc.) on the production of mycelia and exopolysaccharide were observed using a one-factor-at-a-time method. More suitable culture requirements for Mycelial Growth and exopolysaccharide production were proved to be maltose, glycerol, tryptone, soya bean steep powder, yeast extract, medium capacity 200 ml in a 500-ml flask, agitation rate 180 rev min−1, seed age 4–8 days, inoculum size 2·5–7·5% (v/v), etc. The optimal temperatures and initial pHs for Mycelial Growth and exopolysaccharide production were at 26°C and pH 5 and at 28°C and pH 7, respectively, and corresponding optimal culture age were observed to be 8 and 10 days respectively. According to the primary results of the one-factor-at-a-time experiments, the optimal medium for the Mycelial Growth and exopolysaccharide production were obtained using an orthogonal layout method to optimize further. Herein the effects of medium ingredients on the Mycelial Growth of C. jiangxiensis JXPJ 0109 were in the order of yeast extract > tryptone > maltose > CaCl2 > glycerol > MgSO4 > KH2PO4 and the optimal concentration of each composition was 15 g maltose (food-grade), 10 g glycerol, 10 g tryptone, 10 g yeast extract, 1 g KH2PO4, 0·2 g MgSO4, and 0·5 g CaCl2 in 1 l of distilled water, while the order of effects of those components on exopolysaccharide production was yeast extract > maltose > tryptone > glycerol > KH2PO4 > CaCl2 > MgSO4, corresponding to the optimal concentration of medium was as follows: 20 g maltose (food-grade), 8 g glycerol, 5 g tryptone, 10 g yeast extract, 1 g KH2PO4, and 0·5 g CaCl2 in 1 l of distilled water. Conclusions: Under the optimal culture requirements, the maximum exopolysaccharide production reached 3·5 g l−1 after 10 days of fermentation, while the maximum production of Mycelial Growth achieved 14·5 g l−1 after 8 days of fermentation. Significance and Impact of the Study: This is the first report on the submerged culture requirements for Mycelial Growth and exopolysaccharide in C. jiangxiensis, and this two-step optimization strategy in this study can be widely applied to other microbial fermentation processes.
Wang Jian-hua - One of the best experts on this subject based on the ideXlab platform.
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Effect of five Kinds of Fungicides on Mycelial Growth of Trichoderma Viride
Journal of Henan Institute of Science and Technology, 2020Co-Authors: Wang Jian-huaAbstract:The effect of three kinds of fungicides on Mycelial Growth of Trichoderma viride were studied with steel method.The results suffested that the investigated fungicides with different concentration showed different controlling on Mycelial Growth of Trichoedrma viride.The fungicide Jiajing gave completely controlling effect on Mycelial Growth of Trichoderma viride.
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The Influences of Subculture of Cordceps militaris to Mycelial Growth Rate and Esterase Isozyme
Northern Horticulture, 2020Co-Authors: Wang Jian-huaAbstract:Mycelial Growth rate and esterase isozyme of Cordceps militaris were tested by vertical slab polyacrylamide gel electrophoresis and Mycelial Growth rate determination,the aim was to search for the degradation mechanism of Cordceps militaris.The results showed that different esterase isozymes were observed among the different subculture strains and no difference in Mycelial Growth rates.EST isoenzyme showed that 3 EST bands of migration rates 0.63,0.65 and 0.67 were found in all tested strains.F1,F3,F4 and F5 strains showed 2 EST bands,the second and the third band,and the EST bands of F2,F6,F7,F8,F9 varied,the band of migration rate 0.67 disappeared and a new band of 0.63 appeared.
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Effect of chemical inputs on the Mycelial Growth of Stropharia rugoso-annulata
Fujian Agricultural Science and Technology, 2020Co-Authors: Wang Jian-huaAbstract:The effects of pesticides such as deltamethrin,cypermethrin,chlorpyrifos,tricyclazole and fertilizers,including urea,potassium chloride,calcium superphosphate,MAP on the Mycelial Growth of Stropharia rugoso-annulata were studied.The results showed that the Mycelial Growth was significant effected by these pesticides and fertilizers.The Growth of Stropharia rugoso-annulata was inhibited when it cultured on the PSA medium supplemented with 50 mg/kg cypermethrin,50 mg/kg chlorpyrifos,50 mg/kg 20% tricyclazole or 20 mg/kg 75% tricyclazole.In the green food production of Stropharia rugoso-annulata,it suggested that the pesticide residuals in culturing materials needs to be detected and the measures decreasing chemical residuals should be carried out.
