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Inas Nuha Ghaliyah - One of the best experts on this subject based on the ideXlab platform.
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pengaruh polisakarida krestin dari ekstrak coriolus versicolor terhadap jumlah fagosit dan kadar nitrit oksida mus musculus yang diinfeksi Mycobacterium fortuitum
2017Co-Authors: Inas Nuha GhaliyahAbstract:Penelitian ini bertujuan untuk mengetahui pengaruh pemberian polisakarida krestin (PSK) dari ekstrak Coriolus versicolor terhadap jumlah fagosit dan kadar nitrit oksida pada mencit (Mus musculus) yang diinfeksi Mycobacterium fortuitum. Penelitian ini menggunakan 30 mencit (Mus musculus) betina dewasa berumur 8 - 10 minggu dan berat 30 - 40 g. Mencit dibagi atas enam kelompok perlakuan yaitu K (kontrol, tanpa pemberian PSK dan tanpa infeksi bakteri), K+ (kontrol positif, pemberian PSK tanpa diinfeksi M. fortuitum), K- (kontrol negatif, diinfeksi M. fortuitum tanpa pemberian PSK), P1 (pemberian PSK sebelum diinfeksi M. fortuitum), P2 (pemberian PSK sesudah diinfeksi M. fortuitum), dan P3 (pemberian PSK sebelum dan sesudah diinfeksi M. fortuitum). Polisakarida krestin dengan dosis 50 mg/kg BB diberikan secara gavage selama satu minggu. Infeksi mencit oleh Mycobacterium fortuitum (106 bakteri/mL) dilakukan dua kali secara intraperitonial dengan jarak antar infeksi dua minggu. Jumlah fagosit dihitung dengan bilik hitung haemocytometer sedangkan kadar nitrit oksida diukur dengan menggunakan uji nitrit. Data jumlah fagosit dan kadar nitrit dianalisis dengan uji Kolmogorov-Smirnov dan homogenitas variansi dengan α>0,05. Kemudian dilanjutkan dengan uji ANOVA α<0,05 dan uji Duncan α=0,05. Hasil penelitian menunjukkan jumlah fagosit kelompok K 99,4±25,1 sel/mm3, K+ 90,6±29,4 sel/mm3, K- 100,0±35,3 sel/mm3, P1 87,6±43,9 sel/mm3, P2 86,4±17,9 sel/mm3, P3 179,6±36,7 sel/mm3. Nilai kadar nitrit kelompok K 0,496±0,006 M, K+ 0,499±0,006 M, K- 0,475±0,028 M, P1 0,469±0,006 M, P2 0,123±0,026 M, P3 0,384±0,015 M. Peningkatan jumlah fagosit paling tinggi terdapat pada kelompok perlakuan P3 dimana pemberian PSK dilakukan pada saat sebelum dan sesudah infeksi M. fortuitum dan penurunan kadar nitrit oksida paling rendah terdapat pada kelompok perlakuan P2 dimana pemberian PSK dilakukan pada saat sesudah infeksi Mycobacterium fortuitum.
Inas Nuha Ghaliyah 081311433055 - One of the best experts on this subject based on the ideXlab platform.
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PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK Coriolus versicolor TERHADAP JUMLAH FAGOSIT DAN KADAR NITRIT OKSIDA Mus musculus YANG DIINFEKSI Mycobacterium fortuitum
2017Co-Authors: Inas Nuha Ghaliyah 081311433055Abstract:Penelitian ini bertujuan untuk mengetahui pengaruh pemberian polisakarida krestin (PSK) dari ekstrak Coriolus versicolor terhadap jumlah fagosit dan kadar nitrit oksida pada mencit (Mus musculus) yang diinfeksi Mycobacterium fortuitum. Penelitian ini menggunakan 30 mencit (Mus musculus) betina dewasa berumur 8 - 10 minggu dan berat 30 - 40 g. Mencit dibagi atas enam kelompok perlakuan yaitu K (kontrol, tanpa pemberian PSK dan tanpa infeksi bakteri), K+ (kontrol positif, pemberian PSK tanpa diinfeksi M. fortuitum), K- (kontrol negatif, diinfeksi M. fortuitum tanpa pemberian PSK), P1 (pemberian PSK sebelum diinfeksi M. fortuitum), P2 (pemberian PSK sesudah diinfeksi M. fortuitum), dan P3 (pemberian PSK sebelum dan sesudah diinfeksi M. fortuitum). Polisakarida krestin dengan dosis 50 mg/kg BB diberikan secara gavage selama satu minggu. Infeksi mencit oleh Mycobacterium fortuitum (106 bakteri/mL) dilakukan dua kali secara intraperitonial dengan jarak antar infeksi dua minggu. Jumlah fagosit dihitung dengan bilik hitung haemocytometer sedangkan kadar nitrit oksida diukur dengan menggunakan uji nitrit. Data jumlah fagosit dan kadar nitrit dianalisis dengan uji Kolmogorov-Smirnov dan homogenitas variansi dengan α>0,05. Kemudian dilanjutkan dengan uji ANOVA α
Rob J Rentenaar - One of the best experts on this subject based on the ideXlab platform.
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inno lipa dna line probe assay misidentification of m smegmatis as Mycobacterium fortuitum complex
Diagnostic Microbiology and Infectious Disease, 2019Co-Authors: Theo Van Den Broek, Nard Janssen, David J Hetem, Wouter Bekers, Miranda Kamst, Ad C Fluit, Jakko Van Ingen, Johannes G Kusters, Rob J RentenaarAbstract:Abstract Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.
Jay W Kislak - One of the best experts on this subject based on the ideXlab platform.
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pseudo outbreak of Mycobacterium fortuitum due to contaminated ice machines
American Journal of Infection Control, 2002Co-Authors: Vincent J Labombardi, Anne Marie Obrien, Jay W KislakAbstract:Abstract Background: An almost 4-fold increase from normal baseline levels in the isolation of Mycobacterium fortuitum from respiratory tract specimens was observed. The majority of these isolates were obtained from patients residing on 1 of 2 wards, prompting an epidemiologic investigation. Methods: In addition to patient specimens, environmental cultures were collected from various water sources on the 2 affected wards. Samples were also collected from uninvolved areas of the hospital. All specimens were cultured with use of a continuously monitored broth system for the isolation of mycobacteria. Results: The respiratory tracts of 19 patients were colonized by M fortuitum . Surveillance cultures obtained from uninvolved areas of the hospital were either negative for mycobacteria or were colonized by M avium complex or M gordonae . Two ice machines, servicing the affected areas each, were colonized by M fortuitum in multiple cultures. Conclusions: The M fortuitum pseudo-outbreak was due to contaminated ice machines located on each of the affected units. After removal and replacement of the ice machines, the pseudo-outbreak resolved. (Am J Infect Control 2002;30:184-6.)
Laurent Kremer - One of the best experts on this subject based on the ideXlab platform.
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CFTR Depletion Confers Hypersusceptibility to Mycobacterium fortuitum in a Zebrafish Model
Frontiers in Cellular and Infection Microbiology, 2020Co-Authors: Matt Johansen, Laurent KremerAbstract:The Mycobacterium fortuitum complex comprises several closely related species, causing pulmonary and extra-pulmonary infections. However, there is very limited knowledge about the disease pathogenesis involved in M. fortuitum infections, particularly due to the lack of suitable animal models. Using the zebrafish model, we show that embryos are susceptible to M. fortuitum infection in a dose-dependent manner. Furthermore, zebrafish embryos form granulomas from as early as 2 days post-infection, recapitulating critical aspects of mycobacterial pathogenesis observed in other pathogenic species. The formation of extracellular cords in infected embryos highlights a previously unknown pathogenic feature of M. fortuitum. The formation of large corded structures occurs also during in vitro growth, suggesting that this is not a host-adapted stress mechanism deployed during infection. Moreover, transient macrophage depletion led to rapid embryo death with increased extracellular cords, indicating that macrophages are essential determinants of M. fortuitum infection control. Importantly, morpholino depletion of the cystic fibrosis transmembrane conductance regulator (cftr) significantly increased embryo death, bacterial burden, bacterial cords and abscesses. There was a noticeable decrease in the number of cftr-deficient infected embryos with granulomas as compared to infected controls, suggesting that loss of CFTR leads to impaired host immune responses and confers hypersusceptiblity to M. fortuitum infection. Overall, these findings highlight the application of the zebrafish embryo to study M. fortuitum and emphasizes previously unexplored aspects of disease pathogenesis of this significant mycobacterial species.
