The Experts below are selected from a list of 291168 Experts worldwide ranked by ideXlab platform
Michel Drancourt - One of the best experts on this subject based on the ideXlab platform.
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Complete genome sequence of Mycobacterium sp. Strain 3519A
Genome Announcements, 2018Co-Authors: Amar Bouam, Michel Drancourt, Anthony Levasseur, Maryline Bonnet, Laurence Borand, Charles Van Goethem, Sylvain GodreuilAbstract:Mycobacterium sp. strain 3519A is a nontuberculous Mycobacterium isolated from sputum from a Cambodian patient with a pulmonary infection. We report here the first complete 7.3-Mbp-long genome sequence of Mycobacterium sp. 3519A with 66.35% GC content, encoding 7,029 protein-coding genes, 50 tRNAs, and 5 rRNA genes.
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Genome-wide analysis of horizontally acquired genes in the genus Mycobacterium
Scientific Reports, 2018Co-Authors: Arup Panda, Michel Drancourt, Tamir Tuller, Pierre PontarottiAbstract:Horizontal gene transfer (HGT) was attributed as a major driving force for the innovation and evolution of prokaryotic genomes. Previously, multiple research endeavors were undertaken to decipher HGT in different bacterial lineages. The genus Mycobacterium houses some of the most deadly human pathogens; however, the impact of HGT in Mycobacterium has never been addressed in a systematic way. Previous initiatives to explore the genomic imprints of HGTs in Mycobacterium were focused on few selected species, specifically among the members of Mycobacterium tuberculosis complex. Considering the recent availability of a large number of genomes, the current study was initiated to decipher the probable events of HGTs among 109 completely sequenced Mycobacterium species. Our comprehensive phylogenetic analysis with more than 9,000 families of Mycobacterium proteins allowed us to list several instances of gene transfers spread across the Mycobacterium phylogeny. Moreover, by examining the topology of gene phylogenies here, we identified the species most likely to donate and receive these genes and provided a detailed overview of the putative functions these genes may be involved in. Our study suggested that horizontally acquired foreign genes had played an enduring role in the evolution of Mycobacterium genomes and have contributed to their metabolic versatility and pathogenicity.
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Complete Genome Sequence of Mycobacterium sp. Strain 4858
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2018Co-Authors: Amar Bouam, Michel Drancourt, Anthony Levasseur, Maryline Bonnet, Laurence Borand, Charles Van Goethem, Sylvain GodreuilAbstract:Mycobacterium sp. strain 4858 is a nontuberculous Mycobacterium isolated from sputum in a Cambodian patient with a pulmonary infection. We report the first complete 5.6-Mbp-long genome sequence of Mycobacterium strain 4858, with 68.24% GC content, carrying 5,255 protein-coding genes, 47 tRNAs, and 3 rRNA genes.
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Draft Genome Sequence of Mycobacterium porcinum CSURP1564
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2018Co-Authors: Amar Bouam, Anthony Levasseur, Michel DrancourtAbstract:Mycobacterium porcinum is a rapidly growing environmental Mycobacterium responsible for opportunistic infections. The 7,025,616-bp draft genome of M. porcinum strain CSURP1564 exhibits a 66.71% G+C content, 6,687 protein-coding genes, and 65 predicted RNA genes. In silico DNA-DNA hybridization confirms its assignment to the Mycobacterium fortuitum complex.
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Inverse correlation between salt tolerance and host-adaptation in mycobacteria
BMC Research Notes, 2016Co-Authors: Shady Asmar, Mohamed Sassi, Michael Phelippeau, Michel DrancourtAbstract:BACKGROUND: The genus Mycobacterium includes host-adapted organisms regarded as obligate and opportunistic pathogens and environmental organisms. Factors contributing to this wide range of adaptations are poorly known. RESULTS: We studied the salt tolerance of 46 Mycobacterium species of medical interest. Representative strains of the Mycobacterium tuberculosis complex, Mycobacterium avium complex, Mycobacterium chelonae-abscessus complex, Mycobacterium ulcerans, Mycobacterium marinum, Mycobacterium lentiflavum, Mycobacterium fortuitum and Mycobacterium conceptionense were inoculated on Middlebrook 7H10 medium supplemented with 0-10 % sodium chloride. Colonies were counted after 2-4 week incubation at the appropriate 30-37 °C temperature depending on the tested strain. Further comparative genomics was done on 15 Mycobacterium strains representing the spectrum of salt-tolerance of mycobacteria. Based on the results the different species were grouped according to their salt tolerance into a "salt-sensitive" group (growth up to ≤3 % salt) containing the M. tuberculosis complex, Mycobacterium chelonae, Mycobacterium lentiflavum, Mycobacterium ulcerans and Mycobacterium marinum; a "salt-intermediate" group (growth between 4 and 6 % salt) comprising Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium chimaera and a "salt-resistant" group (growth up to \textgreater6 %) comprising Mycobacterium homonissuis, Mycobacterium bolettii, Mycobacterium fortuitum and Mycobacterium conceptionense. Genomic analysis revealed that 290 genes were unique to species belonging to the salt-sensitive group; and that 15 % were annotated as being functionally associated with the ESX secretion systems Pro-Glu and Pro-Pro-Glu family proteins. CONCLUSIONS: In this work we found an inverse correlation between salt tolerance and host adaptation. We thus propose that salinity is one of the multiple factors determining the ecological niches of mycobacteria
Mamadou Daffé - One of the best experts on this subject based on the ideXlab platform.
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a single step sequencing method for the identification of Mycobacterium tuberculosis complex species
PLOS Neglected Tropical Diseases, 2008Co-Authors: Mamadou DafféAbstract:Background The Mycobacterium tuberculosis complex (MTC) comprises closely related species responsible for strictly human and zoonotic tuberculosis. Accurate species determination is useful for the identification of outbreaks and epidemiological links. Mycobacterium africanum and Mycobacterium canettii are typically restricted to Africa and M. bovis is a re-emerging pathogen. Identification of these species is difficult and expensive.
Maria J Garcia - One of the best experts on this subject based on the ideXlab platform.
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whole genome sequence analysis of the Mycobacterium avium complex and proposal of the transfer of Mycobacterium yongonense to Mycobacterium intracellulare subsp yongonense subsp nov
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Maria Castejon, Maria Carmen Menendez, Inaki Comas, Ana Vicente, Maria J GarciaAbstract:Bacterial whole-genome sequences contain informative features of their evolutionary pathways. Comparison of whole-genome sequences have become the method of choice for classification of prokaryotes, thus allowing the identification of bacteria from an evolutionary perspective, and providing data to resolve some current controversies. Currently, controversy exists about the assignment of members of the Mycobacterium avium complex, as is for the cases of Mycobacterium yongonense and ‘Mycobacterium indicus pranii’. These two mycobacteria, closely related to Mycobacterium intracellulare on the basis of standard phenotypic and single gene-sequences comparisons, were not considered a member of such species on the basis on some particular differences displayed by a single strain. Whole-genome sequence comparison procedures, namely the average nucleotide identity and the genome distance, showed that those two mycobacteria should be considered members of the species M. intracellulare . The results were confirmed with other whole-genome comparison supplementary methods. According to the data provided, Mycobacterium yongonense and ‘Mycobacterium indicus pranii’ should be considered and renamed and included as members of M. intracellulare . This study highlights the problems caused when a novel species is accepted on the basis of a single strain, as was the case for M. yongonense . Based mainly on whole-genome sequence analysis, we conclude that M. yongonense should be reclassified as a subspecies of Mycobacterium intracellulare as Mycobacterium intracellulare subsp. yongonense and ‘Mycobacterium indicus pranii’ classified in the same subspecies as the type strain of Mycobacterium intracellulare and classified as Mycobacterium intracellulare subsp. intracellulare.
Maria Castejon - One of the best experts on this subject based on the ideXlab platform.
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whole genome sequence analysis of the Mycobacterium avium complex and proposal of the transfer of Mycobacterium yongonense to Mycobacterium intracellulare subsp yongonense subsp nov
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Maria Castejon, Maria Carmen Menendez, Inaki Comas, Ana Vicente, Maria J GarciaAbstract:Bacterial whole-genome sequences contain informative features of their evolutionary pathways. Comparison of whole-genome sequences have become the method of choice for classification of prokaryotes, thus allowing the identification of bacteria from an evolutionary perspective, and providing data to resolve some current controversies. Currently, controversy exists about the assignment of members of the Mycobacterium avium complex, as is for the cases of Mycobacterium yongonense and ‘Mycobacterium indicus pranii’. These two mycobacteria, closely related to Mycobacterium intracellulare on the basis of standard phenotypic and single gene-sequences comparisons, were not considered a member of such species on the basis on some particular differences displayed by a single strain. Whole-genome sequence comparison procedures, namely the average nucleotide identity and the genome distance, showed that those two mycobacteria should be considered members of the species M. intracellulare . The results were confirmed with other whole-genome comparison supplementary methods. According to the data provided, Mycobacterium yongonense and ‘Mycobacterium indicus pranii’ should be considered and renamed and included as members of M. intracellulare . This study highlights the problems caused when a novel species is accepted on the basis of a single strain, as was the case for M. yongonense . Based mainly on whole-genome sequence analysis, we conclude that M. yongonense should be reclassified as a subspecies of Mycobacterium intracellulare as Mycobacterium intracellulare subsp. yongonense and ‘Mycobacterium indicus pranii’ classified in the same subspecies as the type strain of Mycobacterium intracellulare and classified as Mycobacterium intracellulare subsp. intracellulare.
John A Fuerst - One of the best experts on this subject based on the ideXlab platform.
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diversity of Mycobacterium species from marine sponges and their sensitivity to antagonism by sponge derived rifamycin synthesizing actinobacterium in the genus salinispora
Fems Microbiology Letters, 2010Co-Authors: Hiroshi Izumi, Amitha K Hewavitharana, Nicholas P Shaw, Marie Gauthier, Bernard M Degnan, John A FuerstAbstract:Eleven isolates of Mycobacterium species as well as an antimycobacterial Salinispora arenicola strain were cultured from the sponge Amphimedon queenslandica. The 16S rRNA, rpoB, and hsp65 genes from these Mycobacterium isolates were sequenced, and phylogenetic analysis of a concatenated alignment showed the formation of a large clade with Mycobacterium poriferae isolated previously from another sponge species. The separation of these Mycobacterium isolates into three species-level groups was evident from sequence similarity and phylogenetic analyses. In addition, an isolate that is phylogenetically related to Mycobacterium tuberculosis was recovered from the sponge Fascaplysinopsis sp. Several different mycobacteria thus appear to co-occur in the same sponge. An actinobacterium closely related to S. arenicola, a known producer of the antimycobacterial rifamycins, was coisolated from the same A. queenslandica specimen from which mycobacteria had been isolated. This Salinispora isolate was confirmed to synthesize rifamycin and displayed inhibitory effects against representatives from two of three Mycobacterium phylotype groups. Evidence for antagonism of sponge-derived Salinispora against sponge-derived Mycobacterium strains from the same sponge specimen and the production of antimycobacterial antibiotics by this Salinispora strain suggest that the synthesis of such antibiotics may have functions in competition between sponge microbial community members.
