The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Maria J Garcia - One of the best experts on this subject based on the ideXlab platform.
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whole genome sequence analysis of the Mycobacterium avium complex and proposal of the transfer of Mycobacterium yongonense to Mycobacterium Intracellulare subsp yongonense subsp nov
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Maria Castejon, Maria Carmen Menendez, Inaki Comas, Ana Vicente, Maria J GarciaAbstract:Bacterial whole-genome sequences contain informative features of their evolutionary pathways. Comparison of whole-genome sequences have become the method of choice for classification of prokaryotes, thus allowing the identification of bacteria from an evolutionary perspective, and providing data to resolve some current controversies. Currently, controversy exists about the assignment of members of the Mycobacterium avium complex, as is for the cases of Mycobacterium yongonense and ‘Mycobacterium indicus pranii’. These two mycobacteria, closely related to Mycobacterium Intracellulare on the basis of standard phenotypic and single gene-sequences comparisons, were not considered a member of such species on the basis on some particular differences displayed by a single strain. Whole-genome sequence comparison procedures, namely the average nucleotide identity and the genome distance, showed that those two mycobacteria should be considered members of the species M. Intracellulare . The results were confirmed with other whole-genome comparison supplementary methods. According to the data provided, Mycobacterium yongonense and ‘Mycobacterium indicus pranii’ should be considered and renamed and included as members of M. Intracellulare . This study highlights the problems caused when a novel species is accepted on the basis of a single strain, as was the case for M. yongonense . Based mainly on whole-genome sequence analysis, we conclude that M. yongonense should be reclassified as a subspecies of Mycobacterium Intracellulare as Mycobacterium Intracellulare subsp. yongonense and ‘Mycobacterium indicus pranii’ classified in the same subspecies as the type strain of Mycobacterium Intracellulare and classified as Mycobacterium Intracellulare subsp. Intracellulare.
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Characterization of a Mycobacterium Intracellulare variant strain by molecular techniques.
Journal of Clinical Microbiology, 2001Co-Authors: Maria Carmen Menendez, Elia Palenque, M. C. Navarro, María Núñez, María José Rebollo, Maria J GarciaAbstract:This paper describes a Mycobacterium Intracellulare variant strain causing an unusual infection. Several isolates obtained from an immunocompromised patient were identified as members of the Mycobacterium avium complex (MAC) by the commercial AccuProbe system and biochemical standard identification. Further molecular approaches were undertaken for a more accurate characterization of the bacteria. Up to seven different genomic sequences were analyzed, ranging from conserved mycobacterial genes such as 16S ribosomal DNA to MAC-specific genes such as mig (macrophage-induced gene). The results obtained identify the isolates as a variant of M. Intracellulare, an example of the internal variability described for members of the MAC, particularly within that species. The application of other molecular approaches is recommended for more accurate identification of bacteria described as MAC members.
Yoonhoh Kook - One of the best experts on this subject based on the ideXlab platform.
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Mycobacterium paraIntracellulare sp nov for the genotype int 1 of Mycobacterium Intracellulare
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Che Ok Jeon, Joseph Jeong, Yoonhoh KookAbstract:Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium Intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium Intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. Intracellulare ATCC 13950T. DNA–DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. Intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraIntracellulare . The type strain is MOTT64T (=KCTC 29084T=JCM 30622T).
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Whole-Genome Sequence of Mycobacterium Intracellulare Clinical Strain MOTT-H4Y, Belonging to INT5 Genotype.
Genome Announcements, 2013Co-Authors: Seok Hyun Hong, Yoonhoh KookAbstract:ABSTRACT Here, we report the draft genome sequence of the Mycobacterium Intracellulare clinical strain MOTT-H4Y, grouped previously into the INT5 genotype of the 5 genotypes of M. Intracellulare.
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complete genome sequence of Mycobacterium Intracellulare clinical strain mott 36y belonging to the int5 genotype
Journal of Bacteriology, 2012Co-Authors: Beom Soon Choi, Ik Young Choi, Jongsik Chun, Seok Hyun Hong, Yoonhoh KookAbstract:Here we report the complete genome sequence of the Mycobacterium Intracellulare clinical strain MOTT-36Y, previously grouped into the INT5 genotype among the 5 genotypes of M. Intracellulare. This genome sequence will serve as a valuable reference for understanding the disparity in virulence and epidemiologic traits between M. Intracellulare-related strains.
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complete genome sequence of Mycobacterium Intracellulare strain atcc 13950t
Journal of Bacteriology, 2012Co-Authors: Beom Soon Choi, Ik Young Choi, Jongsik Chun, Yoonhoh KookAbstract:Here we report the first complete genome sequence of Mycobacterium Intracellulare ATCC 13950T, a Mycobacterium avium complex (MAC) strain. This genome sequence will serve as a valuable reference for understanding the epidemiologic, biological, and pathogenic aspects of the disparity between MAC members.
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complete genome sequence of Mycobacterium Intracellulare clinical strain mott 02
Journal of Bacteriology, 2012Co-Authors: Beom Soon Choi, Ik Young Choi, Jongsik Chun, Yoonhoh KookAbstract:Here, we report the first complete genome sequence of the Mycobacterium Intracellulare clinical strain MOTT-02, which was previously grouped in the INT2 genotype of M. Intracellulare. This genome sequence will serve as a valuable reference for improving the understanding of the disparity in the virulence and epidemiologic traits between M. Intracellulare genotypes.
Maria Castejon - One of the best experts on this subject based on the ideXlab platform.
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whole genome sequence analysis of the Mycobacterium avium complex and proposal of the transfer of Mycobacterium yongonense to Mycobacterium Intracellulare subsp yongonense subsp nov
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Maria Castejon, Maria Carmen Menendez, Inaki Comas, Ana Vicente, Maria J GarciaAbstract:Bacterial whole-genome sequences contain informative features of their evolutionary pathways. Comparison of whole-genome sequences have become the method of choice for classification of prokaryotes, thus allowing the identification of bacteria from an evolutionary perspective, and providing data to resolve some current controversies. Currently, controversy exists about the assignment of members of the Mycobacterium avium complex, as is for the cases of Mycobacterium yongonense and ‘Mycobacterium indicus pranii’. These two mycobacteria, closely related to Mycobacterium Intracellulare on the basis of standard phenotypic and single gene-sequences comparisons, were not considered a member of such species on the basis on some particular differences displayed by a single strain. Whole-genome sequence comparison procedures, namely the average nucleotide identity and the genome distance, showed that those two mycobacteria should be considered members of the species M. Intracellulare . The results were confirmed with other whole-genome comparison supplementary methods. According to the data provided, Mycobacterium yongonense and ‘Mycobacterium indicus pranii’ should be considered and renamed and included as members of M. Intracellulare . This study highlights the problems caused when a novel species is accepted on the basis of a single strain, as was the case for M. yongonense . Based mainly on whole-genome sequence analysis, we conclude that M. yongonense should be reclassified as a subspecies of Mycobacterium Intracellulare as Mycobacterium Intracellulare subsp. yongonense and ‘Mycobacterium indicus pranii’ classified in the same subspecies as the type strain of Mycobacterium Intracellulare and classified as Mycobacterium Intracellulare subsp. Intracellulare.
Maria Carmen Menendez - One of the best experts on this subject based on the ideXlab platform.
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whole genome sequence analysis of the Mycobacterium avium complex and proposal of the transfer of Mycobacterium yongonense to Mycobacterium Intracellulare subsp yongonense subsp nov
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Maria Castejon, Maria Carmen Menendez, Inaki Comas, Ana Vicente, Maria J GarciaAbstract:Bacterial whole-genome sequences contain informative features of their evolutionary pathways. Comparison of whole-genome sequences have become the method of choice for classification of prokaryotes, thus allowing the identification of bacteria from an evolutionary perspective, and providing data to resolve some current controversies. Currently, controversy exists about the assignment of members of the Mycobacterium avium complex, as is for the cases of Mycobacterium yongonense and ‘Mycobacterium indicus pranii’. These two mycobacteria, closely related to Mycobacterium Intracellulare on the basis of standard phenotypic and single gene-sequences comparisons, were not considered a member of such species on the basis on some particular differences displayed by a single strain. Whole-genome sequence comparison procedures, namely the average nucleotide identity and the genome distance, showed that those two mycobacteria should be considered members of the species M. Intracellulare . The results were confirmed with other whole-genome comparison supplementary methods. According to the data provided, Mycobacterium yongonense and ‘Mycobacterium indicus pranii’ should be considered and renamed and included as members of M. Intracellulare . This study highlights the problems caused when a novel species is accepted on the basis of a single strain, as was the case for M. yongonense . Based mainly on whole-genome sequence analysis, we conclude that M. yongonense should be reclassified as a subspecies of Mycobacterium Intracellulare as Mycobacterium Intracellulare subsp. yongonense and ‘Mycobacterium indicus pranii’ classified in the same subspecies as the type strain of Mycobacterium Intracellulare and classified as Mycobacterium Intracellulare subsp. Intracellulare.
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Characterization of a Mycobacterium Intracellulare variant strain by molecular techniques.
Journal of Clinical Microbiology, 2001Co-Authors: Maria Carmen Menendez, Elia Palenque, M. C. Navarro, María Núñez, María José Rebollo, Maria J GarciaAbstract:This paper describes a Mycobacterium Intracellulare variant strain causing an unusual infection. Several isolates obtained from an immunocompromised patient were identified as members of the Mycobacterium avium complex (MAC) by the commercial AccuProbe system and biochemical standard identification. Further molecular approaches were undertaken for a more accurate characterization of the bacteria. Up to seven different genomic sequences were analyzed, ranging from conserved mycobacterial genes such as 16S ribosomal DNA to MAC-specific genes such as mig (macrophage-induced gene). The results obtained identify the isolates as a variant of M. Intracellulare, an example of the internal variability described for members of the MAC, particularly within that species. The application of other molecular approaches is recommended for more accurate identification of bacteria described as MAC members.
Che Ok Jeon - One of the best experts on this subject based on the ideXlab platform.
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Mycobacterium paraIntracellulare sp nov for the genotype int 1 of Mycobacterium Intracellulare
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Che Ok Jeon, Joseph Jeong, Yoonhoh KookAbstract:Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium Intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium Intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. Intracellulare ATCC 13950T. DNA–DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of <70 % between one of them and the type strains of M. Intracellulare and Mycobacterium chimaera confirmed their belonging to a distinct species. In addition, phenotypic characteristics such as positive growth on MacConkey agar and in acidic broth culture, unique matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS profiles of lipids, and unique mycolic acids profiles further supported the taxonomic status of these strains as representatives of a novel species of the Mycobacterium avium complex named Mycobacterium paraIntracellulare . The type strain is MOTT64T (=KCTC 29084T=JCM 30622T).
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Mycobacterium paraIntracellulare sp. nov., for the genotype INT-1 of Mycobacterium Intracellulare.
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Che Ok Jeon, Joseph JeongAbstract:Three mycobacterial strains, isolated from independent Korean patients with pulmonary infections, belonging to the Mycobacterium Intracellulare genotype 1 (INT-1) were characterized using a polyphasic approach. The sequences of the 16S rRNA gene and internal transcribed spacer 1 (ITS1) of the INT-1 strains were identical to those of Mycobacterium Intracellulare ATCC 13950T. However, multilocus sequence typing (MLST) analysis targeting five housekeeping genes (hsp65, rpoB, argG, gnd and pgm) revealed the phylogenetic separation of these strains from M. Intracellulare ATCC 13950T. DNA–DNA hybridization values of >70 % confirmed that the three isolates belong to the same species, while the values of
