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Patrik Zanolari - One of the best experts on this subject based on the ideXlab platform.
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diagnostic value of animal side antibody assays for rapid detection of Mycobacterium bovis or Mycobacterium microti infection in south american camelids
Clinical and Vaccine Immunology, 2011Co-Authors: Konstantin P Lyashchenko, Mireille Meylan, Rena Greenwald, Javan Esfandiari, Shelley G Rhodes, G S Dean, Ricardo De La Ruadomenech, Hmartin Vordermeier, Patrik ZanolariAbstract:Tuberculosis (TB) in South American camelids (SAC) is caused by Mycobacterium bovis or Mycobacterium microti. Two serological methods, rapid testing (RT) and the dual-path platform (DPP) assay, were evaluated using naturally infected SAC. The study population included 156 alpacas and 175 llamas in Great Britain, Switzerland, and the United States. TB due to M. bovis (n = 44) or M. microti (n = 8) in 35 alpacas and 17 llamas was diagnosed by gross pathology examination and culture. Control animals were from herds with no TB history. The RT and the DPP assay showed sensitivities of 71% and 74%, respectively, for alpacas, while the sensitivity for llamas was 77% for both assays. The specificity of the DPP assay (98%) was higher than that of RT (94%) for llamas; the specificities of the two assays were identical (98%) for alpacas. When the two antibody tests were combined, the parallel-testing interpretation (applied when either assay produced a positive result) enhanced the sensitivities of antibody detection to 89% for alpacas and 88% for llamas but at the cost of lower specificities (97% and 93%, respectively), whereas the serial-testing interpretation (applied when both assays produced a positive result) maximized the specificity to 100% for both SAC species, although the sensitivities were 57% for alpacas and 65% for llamas. Over 95% of the animals with evidence of TB failed to produce skin test reactions, thus confirming concerns about the validity of this method for testing SAC. The findings suggest that serological assays may offer a more accurate and practical alternative for antemortem detection of camelid TB.
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tuberculosis caused by Mycobacterium microti in south american camelids
Journal of Veterinary Internal Medicine, 2009Co-Authors: Patrik Zanolari, Gaby E Pfyffer, Nadia Robert, Konstantin P Lyashchenko, Rena Greenwald, Javan Esfandiari, Mireille MeylanAbstract:Background: Infection with Mycobacterium microti can cause chronic disease in animals and threaten human health through its zoonotic potential. Objective: To describe clinical findings, diagnostic investigations, necropsy, and epidemiology results in South American camelids (SAC) infected with M. microti, member of the Mycobacterium tuberculosis complex. Animals: Eleven SAC with tuberculous lesions. Methods: Description of 10 llamas and 1 alpaca, aged 4–18 years, from 6 herds with a history of wasting and weakness admitted to the Vetsuisse-Faculty of Berne over 8 years. Results: Clinical signs included weight loss, recumbency, and anorexia in late stages of the disease. Respiratory problems were seen in 6 animals of 11. No consistent hematologic abnormalities were identified. Suspect animals were examined in detail by abdominal ultrasonography and thoracic radiology. Abnormal findings such as enlarged mediastinal, mesenteric, or hepatic lymph nodes were seen only in animals with advanced disease. Single comparative intradermal tuberculin test with bovine protein purified derivate (PPD) and avian PPD was negative in all animals. At necropsy, typical tuberculous lesions were found, and confirmed by bacteriological smear and culture, molecular methods, or both. Conclusions and Clinical Importance: Infection caused by M. microti should be considered a differential diagnosis in chronic debilitating disease with or without respiratory signs in SAC. Antemortem confirmation of the diagnosis remains challenging at any stage of infection. Because cases of M. microti infection have been reported in immunocompromized human patients, the zoonotic potential of the organism should be kept in mind when dealing with this disease in SAC.
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antibody responses in new world camelids with tuberculosis caused by Mycobacterium microti
Veterinary Microbiology, 2007Co-Authors: Konstantin P Lyashchenko, Mireille Meylan, Rena Greenwald, Javan Esfandiari, Hengrave I Burri, Patrik ZanolariAbstract:Antibody responses in New World camelids (NWC) infected with Mycobacterium microti were studied by two serological methods, multiantigen print immunoassay (MAPIA) and lateral-flow-based rapid test (RT). Serum samples were collected during 2004-2006 from 87 animals including 1 alpaca and 7 llamas with confirmed or suspected M. microti infection, 33 potentially exposed but clinically healthy animals from known infected herds, and 46 control NWC from herds where infection had not been previously diagnosed. The serological assays correctly identified infection status in 97% (MAPIA) or 87% (RT) cases. In three llamas with confirmed M. microti infection and one llama with gross pathology suggestive of disease, for which multiple serum samples collected over time were available, the antibody-based tests showed positive results 1-2 years prior to the onset of clinical signs or being found dead. In MAPIA, MPB83 protein was identified to be an immunodominant serological target antigen recognized in NWC infected with M. microti. With the limited number of animals tested in this study, the serological assays demonstrated the potential for convenient, rapid, and accurate diagnosis of M. microti infection in live llamas and alpacas.
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generalized tuberculosis in llamas lama glama due to Mycobacterium microti
Journal of Clinical Microbiology, 2004Co-Authors: Anna Oevermann, Gaby E Pfyffer, Patrik Zanolari, Mireille Meylan, Nadia RobertAbstract:Necropsy of two llamas revealed numerous caseous nodules containing abundant acid-fast bacilli (AFB) in various organs. The AFB were identified by spoligotyping as Mycobacterium microti, vole type. Infection caused by M. microti should be considered in the differential diagnosis of debilitating diseases in New World camelids.
Mireille Meylan - One of the best experts on this subject based on the ideXlab platform.
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diagnostic value of animal side antibody assays for rapid detection of Mycobacterium bovis or Mycobacterium microti infection in south american camelids
Clinical and Vaccine Immunology, 2011Co-Authors: Konstantin P Lyashchenko, Mireille Meylan, Rena Greenwald, Javan Esfandiari, Shelley G Rhodes, G S Dean, Ricardo De La Ruadomenech, Hmartin Vordermeier, Patrik ZanolariAbstract:Tuberculosis (TB) in South American camelids (SAC) is caused by Mycobacterium bovis or Mycobacterium microti. Two serological methods, rapid testing (RT) and the dual-path platform (DPP) assay, were evaluated using naturally infected SAC. The study population included 156 alpacas and 175 llamas in Great Britain, Switzerland, and the United States. TB due to M. bovis (n = 44) or M. microti (n = 8) in 35 alpacas and 17 llamas was diagnosed by gross pathology examination and culture. Control animals were from herds with no TB history. The RT and the DPP assay showed sensitivities of 71% and 74%, respectively, for alpacas, while the sensitivity for llamas was 77% for both assays. The specificity of the DPP assay (98%) was higher than that of RT (94%) for llamas; the specificities of the two assays were identical (98%) for alpacas. When the two antibody tests were combined, the parallel-testing interpretation (applied when either assay produced a positive result) enhanced the sensitivities of antibody detection to 89% for alpacas and 88% for llamas but at the cost of lower specificities (97% and 93%, respectively), whereas the serial-testing interpretation (applied when both assays produced a positive result) maximized the specificity to 100% for both SAC species, although the sensitivities were 57% for alpacas and 65% for llamas. Over 95% of the animals with evidence of TB failed to produce skin test reactions, thus confirming concerns about the validity of this method for testing SAC. The findings suggest that serological assays may offer a more accurate and practical alternative for antemortem detection of camelid TB.
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tuberculosis caused by Mycobacterium microti in south american camelids
Journal of Veterinary Internal Medicine, 2009Co-Authors: Patrik Zanolari, Gaby E Pfyffer, Nadia Robert, Konstantin P Lyashchenko, Rena Greenwald, Javan Esfandiari, Mireille MeylanAbstract:Background: Infection with Mycobacterium microti can cause chronic disease in animals and threaten human health through its zoonotic potential. Objective: To describe clinical findings, diagnostic investigations, necropsy, and epidemiology results in South American camelids (SAC) infected with M. microti, member of the Mycobacterium tuberculosis complex. Animals: Eleven SAC with tuberculous lesions. Methods: Description of 10 llamas and 1 alpaca, aged 4–18 years, from 6 herds with a history of wasting and weakness admitted to the Vetsuisse-Faculty of Berne over 8 years. Results: Clinical signs included weight loss, recumbency, and anorexia in late stages of the disease. Respiratory problems were seen in 6 animals of 11. No consistent hematologic abnormalities were identified. Suspect animals were examined in detail by abdominal ultrasonography and thoracic radiology. Abnormal findings such as enlarged mediastinal, mesenteric, or hepatic lymph nodes were seen only in animals with advanced disease. Single comparative intradermal tuberculin test with bovine protein purified derivate (PPD) and avian PPD was negative in all animals. At necropsy, typical tuberculous lesions were found, and confirmed by bacteriological smear and culture, molecular methods, or both. Conclusions and Clinical Importance: Infection caused by M. microti should be considered a differential diagnosis in chronic debilitating disease with or without respiratory signs in SAC. Antemortem confirmation of the diagnosis remains challenging at any stage of infection. Because cases of M. microti infection have been reported in immunocompromized human patients, the zoonotic potential of the organism should be kept in mind when dealing with this disease in SAC.
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antibody responses in new world camelids with tuberculosis caused by Mycobacterium microti
Veterinary Microbiology, 2007Co-Authors: Konstantin P Lyashchenko, Mireille Meylan, Rena Greenwald, Javan Esfandiari, Hengrave I Burri, Patrik ZanolariAbstract:Antibody responses in New World camelids (NWC) infected with Mycobacterium microti were studied by two serological methods, multiantigen print immunoassay (MAPIA) and lateral-flow-based rapid test (RT). Serum samples were collected during 2004-2006 from 87 animals including 1 alpaca and 7 llamas with confirmed or suspected M. microti infection, 33 potentially exposed but clinically healthy animals from known infected herds, and 46 control NWC from herds where infection had not been previously diagnosed. The serological assays correctly identified infection status in 97% (MAPIA) or 87% (RT) cases. In three llamas with confirmed M. microti infection and one llama with gross pathology suggestive of disease, for which multiple serum samples collected over time were available, the antibody-based tests showed positive results 1-2 years prior to the onset of clinical signs or being found dead. In MAPIA, MPB83 protein was identified to be an immunodominant serological target antigen recognized in NWC infected with M. microti. With the limited number of animals tested in this study, the serological assays demonstrated the potential for convenient, rapid, and accurate diagnosis of M. microti infection in live llamas and alpacas.
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generalized tuberculosis in llamas lama glama due to Mycobacterium microti
Journal of Clinical Microbiology, 2004Co-Authors: Anna Oevermann, Gaby E Pfyffer, Patrik Zanolari, Mireille Meylan, Nadia RobertAbstract:Necropsy of two llamas revealed numerous caseous nodules containing abundant acid-fast bacilli (AFB) in various organs. The AFB were identified by spoligotyping as Mycobacterium microti, vole type. Infection caused by M. microti should be considered in the differential diagnosis of debilitating diseases in New World camelids.
Maria Laura Boschiroli - One of the best experts on this subject based on the ideXlab platform.
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Mycobacterium microti interferes with bovine tuberculosis surveillance
Microorganisms, 2020Co-Authors: Lorraine Michelet, Krystel De Cruz, Sylvie Henault, Jennifer Tambosco, Maria Laura BoschiroliAbstract:Mycobacterium microti, a member of the Mycobacterium tuberculosis complex, was originally described as the cause of tuberculosis in wild rodents. However, in the last few years, an increasing number of cases have been reported in wildlife (wild boars and badgers) and livestock (goat and cattle) in the frame of bovine tuberculosis (bTB) surveillance program, demonstrating the risk of interference with bTB diagnosis in France. In 2019, we detected four cattle infected with M.microti, from three different herds in three different distant regions. For all these cases, ante-mortem diagnosis by the skin test (single intradermal comparative cervical tuberculin (SICCT)) was positive. Confirmation of M.microti infection was based on molecular tests, i.e., specific real-time PCR and spoligotyping. These results highlight a non-negligible risk of interference in the bTB diagnosis system and raise concern about the reliability of diagnostic tests used for bTB surveillance. The use of highly specific tests, like the interferon gamma test (IFN-γ) employed in France or new synthetic specific tuberculins for skin testing could alternatively be used to accurately identify M.bovis (or Mycobacterium caprae) infection at ante-mortem examination. At post-mortem diagnosis, the use of specific molecular tools should be considered to accurately distinguish pathogens within the MTBC and to avoid misleading bTB diagnosis.
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Mycobacterium microti infection in free ranging wild boar spain 2017 2019
Emerging Infectious Diseases, 2019Co-Authors: Bernat Perez De Val, Lorraine Michelet, Maria Laura Boschiroli, Albert Sanz, Merce Soler, Alberto Allepuz, Enric VidalAbstract:Mycobacterium microti is a member of the Mycobacterium tuberculosis complex that causes pathology in many mammals. M. microti infections have been found in some countries in Europe. We report an outbreak of tuberculosis caused by M. microti in wild boars in Spain.
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Mycobacterium microti infection in dairy goats france
Emerging Infectious Diseases, 2016Co-Authors: Lorraine Michelet, Krystel De Cruz, Claudine Karoui, Sylvie Henault, Yohann Phalente, Marina Beral, Maria Laura BoschiroliAbstract:To the Editor: Mycobacterium microti is a member of the Mycobacterium tuberculosis complex (MTBC). This complex also includes M. tuberculosis, which causes human tuberculosis, and M. bovis and M. caprae, which cause bovine tuberculosis. M. microti was initially described as a pathogen of small rodents and also frequently affects domestic animals, especially cats, and has also been described in wildlife, especially wild boars and badgers (1). This Mycobacterium has also been involved in human pulmonary tuberculosis cases, which highlights its potential zoonotic risk (2). We report a case of M. microti infection in a dairy goat herd, which underlines the risk for confounding bovine tuberculosis diagnosis and potential consequences for livestock management. France has been considered officially free of bovine tuberculosis by the European Union since 2001. Surveillance of this disease is based on antemortem testing with tuberculin skin tests and on systematic postmortem inspection at abattoirs through sanitary inspection of carcasses to detect bovine tuberculosis–like lesions. However, because bovine tuberculosis evolves in an insidious manner and antemortem or postmortem diagnostic tests are not efficient for detecting latently infected animals, an infected herd may remain unidentified for long periods and can be responsible for contamination of other herds by animal movement or contact with animals of neighboring herds. For this reason, to detect other potentially associated cases, investigations in herds epidemiologically linked to the index outbreak are also performed, either through skin testing or through diagnostic culling of those animals introduced from the infected herd or any other animal with a skin test–positive result. This case of bovine tuberculosis in a goat was reported in a region of the Alps Mountains in France. The herd was composed of 140 dairy goats, which were used for raw milk cheese production. Goats were semi-extensively bred and kept in pastures >6 months per year. Investigations conducted after identification of a case of bovine tuberculosis in a neighboring cattle herd infected with M. bovis, in which the index case was identified at an abattoir, highlighted the epidemiologic link with the goat herd because animals in both populations shared the same pastures. Thus, the goat herd was subjected to single intradermal tuberculin tests. Three adult goats (goats A, B, and C) showed positive results and were culled for direct diagnosis. Goats A and B showed no lesion at abattoir inspections, but goat C had bovine tuberculosis–like lesions on the retropharyngeal and mediastinal lymph nodes. Retropharyngeal, tracheobronchial, and mediastinal lymph nodes were sampled from the 3 goats. Retromammary lymph nodes were also sampled from goats B and C (both females). Lesions from goat C were examined by histopathologic analysis and showed a profile suggestive of bovine tuberculosis with necrosis, Langhans giant cells, and few acid–alcohol-resistant bacilli by Ziehl-Neelsen staining (Figure). All samples were subjected to bacterial culture and molecular diagnosis (3). Figure Goat lymph node granuloma with numerous Langhans-type multinucleated giant cells from a goat in France infected with Mycobacterium microti (hematoxylin and eosin stain). Scale bar indicates 50 μm. Although after 3 months culture results were negative for all samples, DNA extracted from the retropharyngeal lymph node of goat C showed a positive PCR result for MTBC DNA by the LSI VetMAX Mycobacterium tuberculosis Complex Real-Time PCR Kit (Thermo Fisher Scientific, Villebon sur Yvette, France). Further characterization of this DNA was performed by using molecular analysis specific for the regions of difference, which enables differentiation of MTBC members (4), and spoligotyping (5). The infectious agent from goat C was identified as M. microti spoligotype SB0118. Moreover, a bovine tuberculosis investigation in wildlife (6) identified M. microti spoligotype SB0118 infection case in a dead badger found 8 km from the goat farm. Thus, the long time during which goats remain in pastures might have favored environmental contamination by interaction with wildlife. Furthermore, an additional case of M. microti infection in a cat reported in 2011 in the same region also had the SB0118 spoligotype (7), which demonstrated that this bacillus is actively circulating in animals from this area. M. microti was previously isolated on the basis of a skin test–positive result for cattle in the United Kingdom (8), which demonstrated the risk for infection in livestock. These findings raise concern on reliability of diagnostic tests used for bovine tuberculosis surveillance. M. microti, which is phylogenetically similar to M. bovis or M. caprae and widely disseminated in the environment, could be responsible for misleading diagnostic results, as demonstrated in this study. Highly specific tests are needed to accurately identify M. bovis (or M. caprae) infection at antemortem examination through use of specific antigens, such as ESAT 6 and CFP10, which are absent in M. microti and are currently used in the interferon-γ test in France (9). In addition, at postmortem diagnosis, use of specific molecular tools capable of rapidly distinguishing members of the MTBC should be considered. Histopathologic analysis lacks specificity, and obtaining results for bacterial culture takes too much time for these particularly slow-growing and fastidious mycobacteria. M. microti has already been reported to cause tuberculosis in immunocompromised and immunocompetent patients in France (10). Thus, potential risk for infection of humans by consumption of raw goat milk cheese cannot be ruled out.
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Mycobacterium microti detection in french wildlife
Veterinary Record, 2015Co-Authors: Lorraine Michelet, Krystel De Cruz, Claudine Karoui, Sylvie Henault, Yohann Phalente, Maria Laura BoschiroliAbstract:Mycobacterium microti is a member of the Mycobacterium tuberculosis complex (MTBC), a group of major human and animal pathogens such as Mycobacterium tuberculosis and Mycobacterium bovis . M microti was originally described as the cause of tuberculosis (TB) in wild rodents, but it can also infect other animal species and has even been described as a potential zoonotic agent (Cavanagh and others 2002, Panteix and others 2010). A recent retrospective study reported 35 animal cases in France between 2002 and 2014 (Michelet …
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infection with Mycobacterium microti in animals in france
Journal of Clinical Microbiology, 2015Co-Authors: Lorraine Michelet, Krystel De Cruz, Gina Zanella, Rachid Aaziz, Tabatha Bulach, Claudine Karoui, Sylvie Henault, Guy Joncour, Maria Laura BoschiroliAbstract:We describe here 35 animal cases of tuberculosis due to Mycobacterium microti in France (2002–2014). Recently, molecular tools that overcome the difficulty of confirming infection by this potentially zoonotic agent have revealed an increasing number of cases, suggesting that its prevalence may have been underestimated.
Enric Vidal - One of the best experts on this subject based on the ideXlab platform.
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Mycobacterium microti infection in free ranging wild boar spain 2017 2019
Emerging Infectious Diseases, 2019Co-Authors: Bernat Perez De Val, Lorraine Michelet, Maria Laura Boschiroli, Albert Sanz, Merce Soler, Alberto Allepuz, Enric VidalAbstract:Mycobacterium microti is a member of the Mycobacterium tuberculosis complex that causes pathology in many mammals. M. microti infections have been found in some countries in Europe. We report an outbreak of tuberculosis caused by M. microti in wild boars in Spain.
Stefan Niemann - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic polymorphisms in antibiotic resistance genes of the Mycobacterium tuberculosis complex
The Journal of antimicrobial chemotherapy, 2014Co-Authors: Silke Feuerriegel, Claudio U. Köser, Stefan NiemannAbstract:OBJECTIVES Sequence analysis of known antibiotic resistance genes of the Mycobacterium tuberculosis complex (MTBC) is increasingly being used to infer phenotypic resistance to a variety of antibiotics. However, a clear understanding of the genotype-phenotype relationship is required to interpret genotypic susceptibility results accurately. In this context, it is particularly important to distinguish phylogenetically informative neutral polymorphisms from true resistance-conferring mutations. METHODS Using a collection of 71 strains that encompasses all major MTBC genotypes, we mapped the genetic diversity in 18 genes that are known to be involved or were previously implicated in antibiotic resistance to eight current as well as two novel antibiotics. This included bedaquiline, capreomycin, ethambutol, fluoroquinolones, isoniazid, PA-824, para-aminosalicylic acid, prothionamide, rifampicin and streptomycin. Moreover, we included data from one of our prior studies that focused on two of the three known pyrazinamide resistance genes. RESULTS We found 58 phylogenetic polymorphisms that were markers for the genotypes M. tuberculosis Beijing, Haarlem, Latin American-Mediterranean (LAM), East African Indian (EAI), Delhi/Central Asian (CAS), Ghana, Turkey (Tur), Uganda I and II, Ural and X-type, as well as for Mycobacterium africanum genotypes West African I (WA I) and II (WA II), Mycobacterium bovis, Mycobacterium caprae, Mycobacterium pinnipedii, Mycobacterium microti and Mycobacterium canettii. CONCLUSIONS This study represents one of the most extensive overviews of phylogenetically informative polymorphisms in known resistance genes to date, and will serve as a resource for the design and interpretation of genotypic susceptibility assays.
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landouzy septicemia sepsis tuberculosa acutissima due to Mycobacterium microti in an immunocompetent man
Infection, 2005Co-Authors: H K Geiss, Stefan Niemann, Rita Feldhues, Oliver Nolte, R RiekerAbstract:Even in developed countries, tuberculosis still contributes significantly to morbidity and mortality. The most frequent causative agent is Mycobacterium tuberculosis, while infections due to other mycobacterial species are usually associated with immunocompromised patients. In the following, we describe the case of a previously healthy man who underwent laparotomy for suspected adrenal carcinoma. Peritoneal "cancerous nodules" turned out to be tuberculous granulomas. After surgery the patient developed a protracted septic shock and died 6 days after surgery. Isolation and identification of the causative agent yielded Mycobacterium microti, an uncommon species of the M. tuberculosis complex. No other pathogen could be isolated during the clinical course, which finally led to the diagnosis of Landouzy septicemia (sepsis tuberculosa acutissima).
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bacterial artificial chromosome based comparative genomic analysis identifies Mycobacterium microti as a natural esat 6 deletion mutant
Infection and Immunity, 2002Co-Authors: Priscille Brodin, M Marmiesse, Karin Eiglmeier, Thierry Garnier, Stefan Niemann, Stewart T Cole, Alain Billault, Roland BroschAbstract:Mycobacterium microti is a member of the Mycobacterium tuberculosis complex that causes tuberculosis in voles. Most strains of M. microti are harmless for humans, and some have been successfully used as live tuberculosis vaccines. In an attempt to identify putative virulence factors of the tubercle bacilli, genes that are absent from the avirulent M. microti but present in human pathogen M. tuberculosis or Mycobacterium bovis were searched for. A minimal set of 50 bacterial artificial chromosome (BAC) clones that covers almost all of the genome of M. microti OV254 was constructed, and individual BACs were compared to the corresponding BACs from M. bovis AF2122/97 and M. tuberculosis H37Rv. Comparison of pulsed-field gel-separated DNA digests of BAC clones led to the identification of 10 regions of difference (RD) between M. microti OV254 and M. tuberculosis. A 14-kb chromosomal region (RD1mic) that partly overlaps the RD1 deletion in the BCG vaccine strain was missing from the genomes of all nine tested M. microti strains. This region covers 13 genes, Rv3864 to Rv3876, in M. tuberculosis, including those encoding the potent ESAT-6 and CFP-10 antigens. In contrast, RD5mic, a region that contains three phospholipase C genes (plcA to -C), was missing from only the vole isolates and was present in M. microti strains isolated from humans. Apart from RD1mic and RD5mic other M. microti-specific deleted regions have been identified (MiD1 to MiD3). Deletion of MiD1 has removed parts of the direct repeat region in M. microti and thus contributes to the characteristic spoligotype of M. microti strains.
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Mycobacterium microti llama type infection presenting as pulmonary tuberculosis in a human immunodeficiency virus positive patient
Journal of Clinical Microbiology, 2001Co-Authors: Matthias A Horstkotte, Ingo Sobottka, Carl K Schewe, Peter H Schafer, Rainer Laufs, Sabine Ruschgerdes, Stefan NiemannAbstract:A rare case of Mycobacterium microti infection in a human immunodeficiency virus-positive patient is described. Because of unusual morphological and cultural features, the pathogen was analyzed by spoligotyping and identified as the Mycobacterium microti llama type. Although culture of M. microti is difficult, drug susceptibility testing could be performed, which correlated with the clinical outcome.
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two cases of Mycobacterium microti derived tuberculosis in hiv negative immunocompetent patients
Emerging Infectious Diseases, 2000Co-Authors: Stefan Niemann, Elvira Richter, H Daluggetamm, H Schlesinger, D Graupner, B Konigstein, G Gurath, U Greinert, Sabine RuschgerdesAbstract:We describe two cases of Mycobacterium microti infection causing pulmonary tuberculosis (TB) in HIV-seronegative immunocompetent patients in Germany. The isolates were identified as M. microti of the llama and vole types, according to spoligotype patterns. Our data demonstrate that M. microti can cause severe pulmonary TB in immunocompetent patients.
