The Experts below are selected from a list of 198 Experts worldwide ranked by ideXlab platform
Robert Beabealashvilli - One of the best experts on this subject based on the ideXlab platform.
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Mycoplasma gallisepticum rpoA gene cluster
FEMS microbiology letters, 2002Co-Authors: Andrei Skamrov, Eugenia Feoktistova, Maria Goldman, Robert BeabealashvilliAbstract:Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (rrn23-5), but the genes infA-rpl36-rps13-rpoA-rpl17 are located immediately downstream of the isolated gene for 16S rRNA (rrn16). A new ribosomal protein cluster infA-rpl36-rps13-rpoA-rpl17-rps16-trmD-rpl19 was formed. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed that this ribosomal protein cluster is an operon.
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Mycoplasma gallisepticum 16S rRNA genes
FEMS microbiology letters, 1995Co-Authors: Andrei Skamrov, Marie Goldman, Jana Klasova, Robert BeabealashvilliAbstract:The genome of Mycoplasma gallisepticum A5969 contains a truncated pseudogene for 16S rRNA in addition to a single unsplit rRNA-operon and a second discontinuous set of rRNA genes. Other M. gallisepticum strains tested do not posses the truncated gene. This gene is almost identical to full-size isolated 16S rRNA gene starting from at least 500 nucleotides upstream of the coding sequence and ending at the 977th nucleotide within the structural part of 16S rRNA.
Ren Jia - One of the best experts on this subject based on the ideXlab platform.
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Detection of Mycoplasma gallisepticum by PCR and DNA Probe Hybridzation
Chinese journal of veterinary science, 2000Co-Authors: Ren JiaAbstract:A pair of 25 base primers were designed and synthesized according to the nucleotide fragment(fMG 2) sequence of Mycoplasma gallisepticum. The primers were applied to amplify Mycoplasma gallisepticum DNA by PCR and expected amplification of a 732 bp product was demonstrated by ethidium bromide staining of 1.5% agarose gel electrophoresis,its sensitivity to be 1 pg without the control strain DNA to be amplified.Thereafter the PCR product was used as a probe labelled with DIG 11 dUTP via random primer labelling to hybridize with the DNA above. The Dot blot assay suggested that the probe hybridized with DNA of Mycoplasma gallisepticum but not with that of negative control strains,its sensitivity to be 100 pg.The further detection to naturally infected chickens showed that the two methods developed in this study had a high sensitivity and specificity,which could be applied to detection of early and latent infection of Mycoplasma gallisepticum.
Vadim M. Govorun - One of the best experts on this subject based on the ideXlab platform.
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Transcription profiling data set of different states of Mycoplasma gallisepticum
Elsevier, 2017Co-Authors: Tatiana A. Semashko, Gleb Y. Fisunov, Alexander A. Arzamasov, Vadim M. GovorunAbstract:Mycoplasma gallisepticum belongs to class Mollicutes and causes chronic respiratory disease in birds. It has a reduced genome, lack of cell wall and many metabolic pathways, and also easy to culture and non-pathogenic to humans. Aforementioned made it is a convenient model for studying of systems biology of minimal cell. Studying the transcriptomic level of M. gallisepticum is interesting for both understanding of common principles of transcription regulation of minimal cell and response to definite influence for pathogen bacteria. For rapid investigation of gene expression we developed microarray design including 3366 probes for 678 genes. They included 665 protein coding sequences and 13 antisense RNAs from 816 genes and 17 ncRNAs present in Mycoplasma gallisepticum. The study was performed on Agilent one-color microarray with custom design and random-T7 polymerase primer for cDNA synthesis. Here we present the data for transcription profiling of M. gallisepticum under different types of exposures: genetic knock-out mutants, cell culture exposed to sublethal concentrations of antibiotics and well-characterized heat stress effect. Mutants have transposon insertion to hypothetical membrane protein, lactate dehydrogenase, helicase with unknown function, 1-deoxy-d-xylulose 5-phosphate reductoisomerase or potential sigma factor. For inhibition of important cell systems, treatment with carbonyl cyanide m-chlorophenylhydrazone (CCCP), novobiocin or tetracycline were chosen. Data are available via NCBI Gene Expression Omnibus (GEO) with the accession number GSE85777 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE85777
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Data on genome analysis of Mycoplasma gallisepticum during intracellular infection
Data in brief, 2016Co-Authors: Daria Matyushkina, Gleb Y. Fisunov, Olga Pobeguts, Irina Garanina, Vladislav V. Babenko, Maria T. Vakhitova, Vadim M. GovorunAbstract:Abstract The genus Mycoplasma relates to Gram-positive bacteria that lack a cell wall and are capable to cause chronic disease in humans and animals. Among the agents of infection and disease in domestic poultry and wild birds, Mycoplasma gallisepticum is the most important Mycoplasma species, causing considerable losses in the poultry industry. In the present paper, we provide data on adaptation of M. gallisepticum to the eukaryotic host cells on the genomic level. The major changes were predominantly localized in the VlhA-hemagglutinin genes which are important components of pathogenesis. The ability of Mycoplasmas to change dramatically the repertoire of surface antigens and to vary the immunogenicity of these components allows them to remain undetected by the immune system of the host. The data presented in this article are related to the article entitled “Phase Transition of the Bacterium upon Invasion of a Host Cell as a Mechanism of Adaptation: a Mycoplasma gallisepticum Model.” (Matyushkina et al., 2016) [1] . Data posted in repository https://www.ncbi.nlm.nih.gov/bioproject/315515 . Bioproject ID: PRJNA315515.
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Data on translatome analysis of Mycoplasma gallisepticum.
Data in brief, 2016Co-Authors: Gleb Y. Fisunov, D.v. Evsyutina, Vadim M. GovorunAbstract:Mycoplasma gallisepticum is a bacterium of class Mollicutes which encompasses wall-less bacteria with significantly reduced genomes. Due to their overall reduction and simplicity Mycoplasmas serve as a model of minimal cell and are used for systems biology studies. Here we present raw data on translatome (ribosome-bound mRNA) analysis of Mycoplasma gallisepticum under logarithm growth and heat stress. The data supports the publication of “Ribosomal profiling of Mycoplasma gallisepticum” (G. Y. Fisunov, D. V Evsyutina, A. A. Arzamasov, I. O. Butenko, V. M. Govorun, 2015) [1].
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Proteomic characterization of Mycoplasma gallisepticum nanoforming
Biochemistry. Biokhimiia, 2010Co-Authors: Irina A. Demina, M. A. Rogova, Marina V Serebryakova, Valentina G. Ladygina, Ilya G. Kondratov, A. N. Renteeva, Vadim M. GovorunAbstract:The goal of this work was to create a model for the long persistence of Mycoplasma gallisepticum in depleted medium and under low growth temperature followed by proteomic study of the model. Nanoforms and revertants for M. gallisepticum were obtained. Proteomic maps were produced for different stages of the formation of nanoforms and revertants. It is shown that proteins responsible for essential cellular processes of glycolysis, translation elongation, and DnaK chaperone involved in the stabilization of newly synthesized proteins are crucial for the reversion of M. gallisepticum to a vegetative form. Based on the current data, it is assumed that changes in the metabolism of M. gallisepticum during nanoforming are not post-mortal, thus M. gallisepticum does not transform to uncultivable form, but remains in a reversible dormant state during prolonged unfavorable conditions.
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Proteome of the bacterium Mycoplasma gallisepticum.
Biochemistry. Biokhimiia, 2009Co-Authors: I . A. Demina, M. A. Rogova, D. A. Korzhenevskyi, V.g. Ladygina, Victor G. Zgoda, Marina V Serebryakova, Vadim M. GovorunAbstract:Using modern proteomic assays, we have identified the products of gene expression and posttranslational modifications of proteins of the bacterium Mycoplasma gallisepticum S6. Combinations of different technologies of protein separation by electrophoresis and mass-spectrometric analysis gave us a total of 446 proteins, i.e. 61% of the annotated proteins of this microorganism. The Pro-Q Diamond and Pro-Q Emerald dye technology was used for fluorescent detection of ten phosphoproteins and two glycoproteins. The acylation of proteins was studied by electrophoresis after in vivo labeling with different 14C-labeled fatty acids, followed by autoradiography. Sixteen acylated proteins were identified, with a quarter of them involved in plasma membrane construction and another quarter involved in cell energy metabolism.
Xinyue Shen - One of the best experts on this subject based on the ideXlab platform.
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A SOE-PCR method of introducing multiple mutations into Mycoplasma gallisepticum neuraminidase
Journal of microbiological methods, 2013Co-Authors: Lei Tan, Hongjun Chen, Xusheng Qiu, Cuiping Song, Danqing Chen, Shilei Zhang, Fanqing Zhang, Xinyue ShenAbstract:A modified splicing with overlap extension PCR (SOE-PCR) was generated to introduce 21 TGA to TGG at Mycoplasma gallisepticum MGA_0329 gene. The recombinant protein was successfully expressed and retained neuraminidase activities, indicating that SOE-PCR is a rapid and highly efficient method of introducing multiple mutations into large M. gallisepticum genes.
Andrei Skamrov - One of the best experts on this subject based on the ideXlab platform.
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Mycoplasma gallisepticum rpoA gene cluster
FEMS microbiology letters, 2002Co-Authors: Andrei Skamrov, Eugenia Feoktistova, Maria Goldman, Robert BeabealashvilliAbstract:Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (rrn23-5), but the genes infA-rpl36-rps13-rpoA-rpl17 are located immediately downstream of the isolated gene for 16S rRNA (rrn16). A new ribosomal protein cluster infA-rpl36-rps13-rpoA-rpl17-rps16-trmD-rpl19 was formed. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed that this ribosomal protein cluster is an operon.
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Mycoplasma gallisepticum 16S rRNA genes
FEMS microbiology letters, 1995Co-Authors: Andrei Skamrov, Marie Goldman, Jana Klasova, Robert BeabealashvilliAbstract:The genome of Mycoplasma gallisepticum A5969 contains a truncated pseudogene for 16S rRNA in addition to a single unsplit rRNA-operon and a second discontinuous set of rRNA genes. Other M. gallisepticum strains tested do not posses the truncated gene. This gene is almost identical to full-size isolated 16S rRNA gene starting from at least 500 nucleotides upstream of the coding sequence and ending at the 977th nucleotide within the structural part of 16S rRNA.
