The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform
Laurentiu M Popescu - One of the best experts on this subject based on the ideXlab platform.
-
isolated human uterine telocytes immunocytochemistry and electrophysiology of t type calcium channels
Histochemistry and Cell Biology, 2015Co-Authors: Sanda Maria Cretoiu, Beatrice Mihaela Radu, Adela Banciu, Daniel Dumitru Banciu, Dragos Cretoiu, Laura Cristina Ceafalan, Laurentiu M PopescuAbstract:Recently, telocytes (TCs) were described as a new cell type in the interstitial space of many organs, including Myometrium. TCs are cells with very long, distinctive extensions named telopodes (Tps). It is suggested that TCs play a major role in intercellular signaling, as well as in morphogenesis, especially in morphogenetic bioelectrical signaling. However, TC plasma membrane is yet unexplored regarding the presence and activity of ion channels and pumps. Here, we used a combination of in vitro immunofluorescence and patch-clamp technique to characterize T-type calcium channels in TCs. Myometrial TCs were identified in cell culture (non-pregnant and pregnant Myometrium) as cells having very long Tps and which were positive for CD34 and platelet-derived growth factor receptor-α. Immunofluorescence analysis of the subfamily of T-type (transient) calcium channels CaV3.1 and CaV3.2 presence revealed the expression of these ion channels on the cell body and Tps of non-pregnant and pregnant Myometrium TCs. The expression in TCs from the non-pregnant Myometrium is less intense, being confined to the cell body for CaV3.2, while CaV3.1 was expressed both on the cell body and in Tps. Moreover, the presence of T-type calcium channels in TCs from non-pregnant Myometrium is also confirmed by applying brief ramp depolarization protocols. In conclusion, our results show that T-type calcium channels are present in TCs from human Myometrium and could participate in the generation of endogenous bioelectric signals responsible for the regulation of the surrounding cell behavior, during pregnancy and labor.
-
near infrared low level laser stimulation of telocytes from human Myometrium
Lasers in Medical Science, 2014Co-Authors: Sanda Maria Cretoiu, Beatrice Mihaela Radu, Adela Banciu, Daniel Dumitru Banciu, Dragos Cretoiu, Razvanalexandru Campeanu, Laurentiu M PopescuAbstract:Telocytes (TCs) are a brand-new cell type frequently observed in the interstitial space of many organs (see www.telocytes.com). TCs are defined by very long (tens of micrometers) and slender prolongations named telopodes. At their level, dilations—called podoms (~300 nm), alternate with podomers (80–100 nm). TCs were identified in a myometrial interstitial cell culture based on morphological criteria and by CD34 and PDGF receptor alpha (PDGFRα) immunopositivity. However, the mechanism(s) of telopodes formation and/or elongation and ramification is not known. We report here the low-level laser stimulation (LLLS) using a 1,064-nm neodymium-doped yttrium aluminum garnet (Nd:YAG) laser (with an output power of 60 mW) of the telopodal lateral extension (TLE) growth in cell culture. LLLS of TCs determines a higher growth rate of TLE in pregnant Myometrium primary cultures (10.3 ± 1.0 μm/min) compared to nonpregnant ones (6.6 ± 0.9 μm/min). Acute exposure (30 min) of TCs from pregnant Myometrium to 1 μM mibefradil, a selective inhibitor of T-type calcium channels, determines a significant reduction in the LLLS TLE growth rate (5.7 ± 0.8 μm/min) compared to LLLS per se in same type of samples. Meanwhile, chronic exposure (24 h) completely abolishes the LLLS TLE growth in both nonpregnant and pregnant myometria. The initial direction of TLE growth was modified by LLLS, the angle of deviation being more accentuated in TCs from human pregnant Myometrium than in TCs from nonpregnant Myometrium. In conclusion, TCs from pregnant Myometrium are more susceptible of reacting to LLLS than those from nonpregnant Myometrium. Therefore, some implications are emerging for low-level laser therapy (LLLT) in uterine regenerative medicine.
Ian S Fraser - One of the best experts on this subject based on the ideXlab platform.
-
effects of hormonal treatment on nerve fibers in endometrium and Myometrium in women with endometriosis
Fertility and Sterility, 2008Co-Authors: Natsuko Tokushige, Robert Markham, Peter Russell, Ian S FraserAbstract:Objective To investigate how hormonal treatment can change nerve fiber density and to identify types of nerve fibers in endometrium and Myometrium in women with endometriosis. Design Laboratory study using human tissue. Setting University-based laboratory. Patient(s) Hormonally treated and untreated women with endometriosis undergoing hysterectomy or curettage. Intervention(s) Endometrial and myometrial tissues were prepared from women with hormonally treated endometriosis and women with untreated endometriosis. Main Outcome Measure(s) Types and density of nerve fibers in endometrium and Myometrium in women with hormonally treated and untreated endometriosis were determined immunohistochemically. Result(s) The nerve fiber density (mean density ± SD per square millimeter) in the functional and the basal layers of endometrium (0.2 ± 0.7/mm2 and 0.9 ± 1.3/mm2, respectively) and Myometrium (1.5 ± 0.8/mm2) from women with hormonally treated endometriosis was much lower than that of endometrium (functional layer: 11 ± 5/mm2, basal layer: 18 ± 8/mm2, respectively) and Myometrium (3 ± 1/mm2) from women with untreated endometriosis. Nerve growth factor and nerve growth factor receptor p75 expression was also significantly reduced in women with hormonally treated endometriosis compared with women with untreated endometriosis. Conclusion(s) Hormonal treatment significantly reduced nerve fiber density in endometrium and Myometrium in women with endometriosis.
-
different types of small nerve fibers in eutopic endometrium and Myometrium in women with endometriosis
Fertility and Sterility, 2007Co-Authors: Natsuko Tokushige, Robert Markham, Peter Russell, Ian S FraserAbstract:OBJECTIVE: To investigate types of nerve fibers in endometrium and Myometrium in women with endometriosis. DESIGN: Laboratory study using human tissue. SETTING: University-based laboratory. PATIENT(S): Women with and without endometriosis undergoing hysterectomy. INTERVENTION(S): Histologic sections of contiguous endometrial and myometrial tissues were prepared from hysterectomies performed on women with and without endometriosis. MAIN OUTCOME MEASURE(S): Types and density of nerve fibers in endometrium and Myometrium in women with and without endometriosis were determined using a series of specific markers for neuronal structure and function: PGP9.5, NF, SP, CGRP, TH, VAChT, VIP, and NPY. RESULT(S): Nerve fibers stained with PGP9.5 and NF in endometrium and Myometrium were significantly increased in women with endometriosis compared with women without endometriosis. Nerve fibers in the functional layer of endometrium in women with endometriosis were likely to be sensory C, a mixture of sensory A delta, sensory C, and adrenergic fibers in the basal layer of the endometrium, a mixture of sensory A delta, sensory C, adrenergic and cholinergic fibers in the Myometrium. CONCLUSION(S): Increased nerve fiber density in endometrium and Myometrium, and sensory C fibers and adrenergic nerve fibers in the endometrium in women with endometriosis may play an important role in the mechanisms of pain generation in this condition.
Beatrice Mihaela Radu - One of the best experts on this subject based on the ideXlab platform.
-
isolated human uterine telocytes immunocytochemistry and electrophysiology of t type calcium channels
Histochemistry and Cell Biology, 2015Co-Authors: Sanda Maria Cretoiu, Beatrice Mihaela Radu, Adela Banciu, Daniel Dumitru Banciu, Dragos Cretoiu, Laura Cristina Ceafalan, Laurentiu M PopescuAbstract:Recently, telocytes (TCs) were described as a new cell type in the interstitial space of many organs, including Myometrium. TCs are cells with very long, distinctive extensions named telopodes (Tps). It is suggested that TCs play a major role in intercellular signaling, as well as in morphogenesis, especially in morphogenetic bioelectrical signaling. However, TC plasma membrane is yet unexplored regarding the presence and activity of ion channels and pumps. Here, we used a combination of in vitro immunofluorescence and patch-clamp technique to characterize T-type calcium channels in TCs. Myometrial TCs were identified in cell culture (non-pregnant and pregnant Myometrium) as cells having very long Tps and which were positive for CD34 and platelet-derived growth factor receptor-α. Immunofluorescence analysis of the subfamily of T-type (transient) calcium channels CaV3.1 and CaV3.2 presence revealed the expression of these ion channels on the cell body and Tps of non-pregnant and pregnant Myometrium TCs. The expression in TCs from the non-pregnant Myometrium is less intense, being confined to the cell body for CaV3.2, while CaV3.1 was expressed both on the cell body and in Tps. Moreover, the presence of T-type calcium channels in TCs from non-pregnant Myometrium is also confirmed by applying brief ramp depolarization protocols. In conclusion, our results show that T-type calcium channels are present in TCs from human Myometrium and could participate in the generation of endogenous bioelectric signals responsible for the regulation of the surrounding cell behavior, during pregnancy and labor.
-
near infrared low level laser stimulation of telocytes from human Myometrium
Lasers in Medical Science, 2014Co-Authors: Sanda Maria Cretoiu, Beatrice Mihaela Radu, Adela Banciu, Daniel Dumitru Banciu, Dragos Cretoiu, Razvanalexandru Campeanu, Laurentiu M PopescuAbstract:Telocytes (TCs) are a brand-new cell type frequently observed in the interstitial space of many organs (see www.telocytes.com). TCs are defined by very long (tens of micrometers) and slender prolongations named telopodes. At their level, dilations—called podoms (~300 nm), alternate with podomers (80–100 nm). TCs were identified in a myometrial interstitial cell culture based on morphological criteria and by CD34 and PDGF receptor alpha (PDGFRα) immunopositivity. However, the mechanism(s) of telopodes formation and/or elongation and ramification is not known. We report here the low-level laser stimulation (LLLS) using a 1,064-nm neodymium-doped yttrium aluminum garnet (Nd:YAG) laser (with an output power of 60 mW) of the telopodal lateral extension (TLE) growth in cell culture. LLLS of TCs determines a higher growth rate of TLE in pregnant Myometrium primary cultures (10.3 ± 1.0 μm/min) compared to nonpregnant ones (6.6 ± 0.9 μm/min). Acute exposure (30 min) of TCs from pregnant Myometrium to 1 μM mibefradil, a selective inhibitor of T-type calcium channels, determines a significant reduction in the LLLS TLE growth rate (5.7 ± 0.8 μm/min) compared to LLLS per se in same type of samples. Meanwhile, chronic exposure (24 h) completely abolishes the LLLS TLE growth in both nonpregnant and pregnant myometria. The initial direction of TLE growth was modified by LLLS, the angle of deviation being more accentuated in TCs from human pregnant Myometrium than in TCs from nonpregnant Myometrium. In conclusion, TCs from pregnant Myometrium are more susceptible of reacting to LLLS than those from nonpregnant Myometrium. Therefore, some implications are emerging for low-level laser therapy (LLLT) in uterine regenerative medicine.
Bo Lindblom - One of the best experts on this subject based on the ideXlab platform.
-
sex steroid receptors in human Myometrium and fibroids changes during the menstrual cycle and gonadotropin releasing hormone treatment
The Journal of Clinical Endocrinology and Metabolism, 1998Co-Authors: Katarina Englund, Agneta Blanck, Inger Gustavsson, Ulrika Lundkvist, Peter Sjoblom, Allan Norgren, Bo LindblomAbstract:The content of estrogen and progesterone receptors (ER, PR) is higher in fibroid tissue than in homologous Myometrium, and both receptors seem to be regulated by the levels of circulating sex steroids. Myometrial and fibroid tissues were recovered from women undergoing gynecological operations during different phases of the menstrual cycle and during treatment with an analogue of GnRH (GnRHa). Contents of ER and PR in the tissue cytosol were determined by enzyme immunoassay. The ER levels were significantly higher in fibroid tissue than in homologous Myometrium in all the endocrine conditions. During the secretory phase, when luteal progesterone production is prominent, the ER levels in the Myometrium and fibroids were lower than during the proliferative phase. During GnRHa treatment, the ER levels in both tissues were similar to those in the proliferative phase but significantly higher than in the secretory phase. The PR levels were also significantly higher in fibroids than in Myometrium in all the diff...
Xuesen Dong - One of the best experts on this subject based on the ideXlab platform.
-
hoxa10 and hoxa11 regulate the expression of contraction associated proteins and contribute to regionalized Myometrium phenotypes in women
Reproductive Sciences, 2018Co-Authors: Yinan Li, Haolong Li, Ruiqi Chen, Donna M Slater, Xuesen DongAbstract:A relaxed fundus (FUN) and a contracted lower uterine segment (LUS) of human Myometrium are required for maintaining pregnancy. How this regional Myometrium function is regulated remains unclear. W...
