The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
Lois A. Salamonsen - One of the best experts on this subject based on the ideXlab platform.
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idiopathic infertility in women is associated with distinct changes in Proliferative Phase uterine fluid proteins
Biology of Reproduction, 2018Co-Authors: Harriet Fitzgerald, Jemma Evans, Nicholas Johnson, Giuseppe Infusini, Andrew I. Webb, Luk Rombauts, Beverley Vollenhoven, Lois A. SalamonsenAbstract:The regenerative, Proliferative Phase of a woman's menstrual cycle is a critical period which lays the foundation for the subsequent, receptive secretory Phase. Although endometrial glands and their secretions are essential for embryo implantation and survival, the Proliferative Phase, when these glands form, has been rarely examined. We hypothesized that alterations in the secreted proteome of the endometrium of idiopathic infertile women would reflect a disturbance in Proliferative Phase endometrial regeneration. Our aim was to compare the proteomic profile of Proliferative Phase uterine fluid from fertile (n = 9) and idiopathic infertile (n = 10) women. Proteins with ≥2-fold change (P < 0.05) were considered significantly altered between fertile and infertile groups. Immunohistochemistry examined the endometrial localization of identified proteins. Western immunoblotting defined the forms of extracellular matrix protein 1 (ECM1) in uterine lavage fluid. Proteomic analysis identified four proteins significantly downregulated in infertile women compared to fertile women, including secreted frizzled-related protein 4 (SFRP4), CD44, and ECM1: two proteins were upregulated. Seven proteins were unique to the fertile group and six (including isoaspartyl peptidase/L-asparaginase [ASRGL1]) were unique to the infertile group. Identified proteins were classified into biological processes of tissue regeneration and regulatory processes. ASRGL1, SFRP4, and ECM1 localized to glandular epithelium and stroma, cluster of differentiation 44 (CD44) to stroma and immune cells. ECM1 was present in two main molecular weight forms in uterine fluid. Our results indicate a disturbance in endometrial development during the Proliferative Phase among infertile women, providing insights into human endometrial development and potential therapeutic targets for infertility.
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Idiopathic infertility in women is associated with distinct changes in Proliferative Phase uterine fluid proteins.
Biology of reproduction, 2018Co-Authors: Harriet Fitzgerald, Jemma Evans, Nicholas Johnson, Giuseppe Infusini, Andrew I. Webb, Luk Rombauts, Beverley Vollenhoven, Lois A. Salamonsen, Tracey A. EdgellAbstract:The regenerative, Proliferative Phase of a woman's menstrual cycle is a critical period which lays the foundation for the subsequent, receptive secretory Phase. Although endometrial glands and their secretions are essential for embryo implantation and survival, the Proliferative Phase, when these glands form, has been rarely examined. We hypothesized that alterations in the secreted proteome of the endometrium of idiopathic infertile women would reflect a disturbance in Proliferative Phase endometrial regeneration. Our aim was to compare the proteomic profile of Proliferative Phase uterine fluid from fertile (n = 9) and idiopathic infertile (n = 10) women. Proteins with ≥2-fold change (P
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the Proliferative Phase underpins endometrial development altered cytokine profiles in uterine lavage fluid of women with idiopathic infertility
Cytokine, 2016Co-Authors: Harriet Fitzgerald, Beverley Vollenhoven, Lois A. Salamonsen, Luk J.r. Rombauts, Tracey A. EdgellAbstract:Endometrial gland development occurs during the Proliferative Phase of a woman's menstrual cycle, laying the foundation for the subsequent receptive, secretory Phase when pregnancy is established. Idiopathic infertility has been rarely investigated with respect to the Proliferative Phase endometrium. We investigated whether gland development and/or altered secretion of cytokines during the Proliferative Phase is associated with infertility. Area of the glandular epithelium (GE) was measured in Proliferative Phase endometrial tissue collected from fertile (n=18) and infertile (n=14) women. Cytokines were measured in Proliferative Phase uterine lavage of fertile (n=15) and infertile (n=15) women. Immunohistochemistry determined cellular localisation of transforming growth factor alpha (TGFα) and interferon gamma (IFNγ) in Proliferative Phase endometrial tissue. For statistical analysis the cohort was divided into women <35years and ⩾35years. There were no significant differences in GE area of infertile and fertile women. C-C motif chemokine 11 (P=0.048), TGFα (P=0.049), IFNγ (P=0.033) and interleukin-1 alpha (P=0.047) were significantly elevated in uterine lavage from infertile women <35years compared to fertile but not in women ⩾35years. TGFα and IFNγ localised predominantly to GE in both the fertile and infertile endometrium. The potential impact of this altered Proliferative Phase environment on subsequent receptivity is discussed.
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The Proliferative Phase underpins endometrial development: Altered cytokine profiles in uterine lavage fluid of women with idiopathic infertility.
Cytokine, 2016Co-Authors: Harriet Fitzgerald, Beverley Vollenhoven, Lois A. Salamonsen, Luk J.r. Rombauts, Tracey A. EdgellAbstract:Endometrial gland development occurs during the Proliferative Phase of a woman's menstrual cycle, laying the foundation for the subsequent receptive, secretory Phase when pregnancy is established. Idiopathic infertility has been rarely investigated with respect to the Proliferative Phase endometrium. We investigated whether gland development and/or altered secretion of cytokines during the Proliferative Phase is associated with infertility. Area of the glandular epithelium (GE) was measured in Proliferative Phase endometrial tissue collected from fertile (n=18) and infertile (n=14) women. Cytokines were measured in Proliferative Phase uterine lavage of fertile (n=15) and infertile (n=15) women. Immunohistochemistry determined cellular localisation of transforming growth factor alpha (TGFα) and interferon gamma (IFNγ) in Proliferative Phase endometrial tissue. For statistical analysis the cohort was divided into women
Harriet Fitzgerald - One of the best experts on this subject based on the ideXlab platform.
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idiopathic infertility in women is associated with distinct changes in Proliferative Phase uterine fluid proteins
Biology of Reproduction, 2018Co-Authors: Harriet Fitzgerald, Jemma Evans, Nicholas Johnson, Giuseppe Infusini, Andrew I. Webb, Luk Rombauts, Beverley Vollenhoven, Lois A. SalamonsenAbstract:The regenerative, Proliferative Phase of a woman's menstrual cycle is a critical period which lays the foundation for the subsequent, receptive secretory Phase. Although endometrial glands and their secretions are essential for embryo implantation and survival, the Proliferative Phase, when these glands form, has been rarely examined. We hypothesized that alterations in the secreted proteome of the endometrium of idiopathic infertile women would reflect a disturbance in Proliferative Phase endometrial regeneration. Our aim was to compare the proteomic profile of Proliferative Phase uterine fluid from fertile (n = 9) and idiopathic infertile (n = 10) women. Proteins with ≥2-fold change (P < 0.05) were considered significantly altered between fertile and infertile groups. Immunohistochemistry examined the endometrial localization of identified proteins. Western immunoblotting defined the forms of extracellular matrix protein 1 (ECM1) in uterine lavage fluid. Proteomic analysis identified four proteins significantly downregulated in infertile women compared to fertile women, including secreted frizzled-related protein 4 (SFRP4), CD44, and ECM1: two proteins were upregulated. Seven proteins were unique to the fertile group and six (including isoaspartyl peptidase/L-asparaginase [ASRGL1]) were unique to the infertile group. Identified proteins were classified into biological processes of tissue regeneration and regulatory processes. ASRGL1, SFRP4, and ECM1 localized to glandular epithelium and stroma, cluster of differentiation 44 (CD44) to stroma and immune cells. ECM1 was present in two main molecular weight forms in uterine fluid. Our results indicate a disturbance in endometrial development during the Proliferative Phase among infertile women, providing insights into human endometrial development and potential therapeutic targets for infertility.
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Idiopathic infertility in women is associated with distinct changes in Proliferative Phase uterine fluid proteins.
Biology of reproduction, 2018Co-Authors: Harriet Fitzgerald, Jemma Evans, Nicholas Johnson, Giuseppe Infusini, Andrew I. Webb, Luk Rombauts, Beverley Vollenhoven, Lois A. Salamonsen, Tracey A. EdgellAbstract:The regenerative, Proliferative Phase of a woman's menstrual cycle is a critical period which lays the foundation for the subsequent, receptive secretory Phase. Although endometrial glands and their secretions are essential for embryo implantation and survival, the Proliferative Phase, when these glands form, has been rarely examined. We hypothesized that alterations in the secreted proteome of the endometrium of idiopathic infertile women would reflect a disturbance in Proliferative Phase endometrial regeneration. Our aim was to compare the proteomic profile of Proliferative Phase uterine fluid from fertile (n = 9) and idiopathic infertile (n = 10) women. Proteins with ≥2-fold change (P
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the Proliferative Phase underpins endometrial development altered cytokine profiles in uterine lavage fluid of women with idiopathic infertility
Cytokine, 2016Co-Authors: Harriet Fitzgerald, Beverley Vollenhoven, Lois A. Salamonsen, Luk J.r. Rombauts, Tracey A. EdgellAbstract:Endometrial gland development occurs during the Proliferative Phase of a woman's menstrual cycle, laying the foundation for the subsequent receptive, secretory Phase when pregnancy is established. Idiopathic infertility has been rarely investigated with respect to the Proliferative Phase endometrium. We investigated whether gland development and/or altered secretion of cytokines during the Proliferative Phase is associated with infertility. Area of the glandular epithelium (GE) was measured in Proliferative Phase endometrial tissue collected from fertile (n=18) and infertile (n=14) women. Cytokines were measured in Proliferative Phase uterine lavage of fertile (n=15) and infertile (n=15) women. Immunohistochemistry determined cellular localisation of transforming growth factor alpha (TGFα) and interferon gamma (IFNγ) in Proliferative Phase endometrial tissue. For statistical analysis the cohort was divided into women <35years and ⩾35years. There were no significant differences in GE area of infertile and fertile women. C-C motif chemokine 11 (P=0.048), TGFα (P=0.049), IFNγ (P=0.033) and interleukin-1 alpha (P=0.047) were significantly elevated in uterine lavage from infertile women <35years compared to fertile but not in women ⩾35years. TGFα and IFNγ localised predominantly to GE in both the fertile and infertile endometrium. The potential impact of this altered Proliferative Phase environment on subsequent receptivity is discussed.
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The Proliferative Phase underpins endometrial development: Altered cytokine profiles in uterine lavage fluid of women with idiopathic infertility.
Cytokine, 2016Co-Authors: Harriet Fitzgerald, Beverley Vollenhoven, Lois A. Salamonsen, Luk J.r. Rombauts, Tracey A. EdgellAbstract:Endometrial gland development occurs during the Proliferative Phase of a woman's menstrual cycle, laying the foundation for the subsequent receptive, secretory Phase when pregnancy is established. Idiopathic infertility has been rarely investigated with respect to the Proliferative Phase endometrium. We investigated whether gland development and/or altered secretion of cytokines during the Proliferative Phase is associated with infertility. Area of the glandular epithelium (GE) was measured in Proliferative Phase endometrial tissue collected from fertile (n=18) and infertile (n=14) women. Cytokines were measured in Proliferative Phase uterine lavage of fertile (n=15) and infertile (n=15) women. Immunohistochemistry determined cellular localisation of transforming growth factor alpha (TGFα) and interferon gamma (IFNγ) in Proliferative Phase endometrial tissue. For statistical analysis the cohort was divided into women
Patrick G. Groothuis - One of the best experts on this subject based on the ideXlab platform.
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Expression and regulation of vascular endothelial growth factor ligands and receptors during menstruation and post-menstrual repair of human endometrium
Molecular human reproduction, 2006Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, Rick Kamps, A.f.p.m. De Goeij, Victor L. Thijssen, S. Tchaikovski, Bert Delvoux, Arjan W. Griffioen, Patrick G. GroothuisAbstract:Regeneration and growth of the human endometrium after shedding of the functional layer during menstruation depends on an adequate angiogenic response. We analysed the mRNA expression levels of all known vascular endothelial growth factor (VEGF) ligands and receptors in human endometrium collected in the menstrual and Proliferative Phases of the menstrual cycle. In addition, we evaluated the expression of VEGF-A, VEGF-R2 and NRP-1 at the protein level. Two periods of elevated mRNA expression of ligands and receptors were observed, separated by a distinct drop at cycle days (CDs) 9 and 10. Immunohistochemical staining showed that VEGF and VEGF-R2 were expressed in epithelial, stromal and endothelial cells. NRP-1 was mainly confined to stroma and blood vessels; only in late-Proliferative endometrium, epithelial staining was also observed. Except for endothelial VEGF-R2 expression in CDs 6-8, there were no significant differences in the expression of VEGF, VEGF-R2 or NRP-1 in any of the cell compartments. In contrast, VEGF release by cultured human endometrium explants decreased during the Proliferative Phase. This output was significantly reduced in menstrual and early-Proliferative endometrium by estradiol (E2) treatment. Western blot analysis indicated that part of the VEGF-A was trapped in the extracellular matrix (ECM). Changes in VEGF ligands and receptors were associated with elevated expression of the hypoxia markers HIF1alpha and CA-IX in the menstrual and early Proliferative Phases. HIF1alpha was also detected in late-Proliferative Phase endometrium. Our findings indicate that VEGF-A exerts its actions mostly during the first half of the Proliferative Phase. Furthermore, VEGF-A production appears to be triggered by hypoxia in the menstrual Phase and subsequently suppressed by estrogen during the late Proliferative Phase.
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Expression and regulation of vascular endothelial growth factor ligands and receptors during menstruation and post-menstrual repair of human endometrium
2006Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, Rick Kamps, A.f.p.m. De Goeij, Victor L. Thijssen, S. Tchaikovski, Bert Delvoux, Arjan W. Griffioen, Patrick G. GroothuisAbstract:*This article is free to read on the publisher's website* Regeneration and growth of the human endometrium after shedding of the functional layer during menstruation depends on an adequate angiogenic response. We analysed the mRNA expression levels of all known vascular endothelial growth factor (VEGF) ligands and receptors in human endometrium collected in the menstrual and Proliferative Phases of the menstrual cycle. In addition, we evaluated the expression of VEGF-A, VEGF-R2 and NRP-1 at the protein level. Two periods of elevated mRNA expression of ligands and receptors were observed, separated by a distinct drop at cycle days (CDs) 9 and 10. Immunohistochemical staining showed that VEGF and VEGF-R2 were expressed in epithelial, stromal and endothelial cells. NRP-1 was mainly confined to stroma and blood vessels; only in late-Proliferative endometrium, epithelial staining was also observed. Except for endothelial VEGF-R2 expression in CDs 6-8, there were no significant differences in the expression of VEGF, VEGF-R2 or NRP-1 in any of the cell compartments. In contrast, VEGF release by cultured human endometrium explants decreased during the Proliferative Phase. This output was significantly reduced in menstrual and early-Proliferative endometrium by estradiol (E2) treatment. Western blot analysis indicated that part of the VEGF-A was trapped in the extracellular matrix (ECM). Changes in VEGF ligands and receptors were associated with elevated expression of the hypoxia markers HIF1 alpha and CA-IX in the menstrual and early Proliferative Phases. HIF1 alpha was also detected in late-Proliferative Phase endometrium. Our findings indicate that VEGF-A exerts its actions mostly during the first half of the Proliferative Phase. Furthermore, VEGF-A production appears to be triggered by hypoxia in the menstrual Phase and subsequently suppressed toy estrogen during the late Proliferative Phase.
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Triphasic pattern in the ex vivo response of human Proliferative Phase endometrium to oestrogens.
The Journal of steroid biochemistry and molecular biology, 2004Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, E. Marbaix, Rick Kamps, C. Galant, Annemiek W. Nap, A.f.p.m. De Goeij, A. Ederveen, Patrick G. GroothuisAbstract:The aim of this study was to evaluate the ex vivo oestrogen responsiveness of human Proliferative Phase endometrium using short-term explant cultures. The effects of oestrogen (17β-E2) on proliferation and the expression of oestrogen-responsive genes known to be involved in regulating endometrial function were evaluated. Three distinct response patterns could be distinguished: (1) the menstrual (M) Phase pattern (cycle days 2-5), which is characterised by a complete lack in the Proliferative response to 17β-E2, while an increased expression of AR (2.6-fold, P < 0.01), PR (2.7-fold, P < 0.01) and COX-2 (3.5-fold, P < 0.01) at the mRNA level was observed and a similar upregulation was also found for AR, PR and COX-2 at the protein level; (2) the early Proliferative (EP) Phase pattern (cycle days 6-10) with 17β-E2 enhanced proliferation in the stroma (1.7-fold, P < 0.05), whereas the expression of AR, PR and COX-2 were not affected at the mRNA and protein levels and ER-α mRNA and protein levels were significantly reduced by 17β-E2; (3) the late Proliferative (LP) Phase pattern (cycle days 11-14), which is characterised by a moderate stimulation of proliferation (1.4-fold, P < 0.05) and PR mRNA expression (1.7-fold, P < 0.01) by 17β-E2. In conclusion, three distinct response patterns to 17β-E2 could be identified with respect to proliferation and the expression of known oestrogen-responsive genes in human Proliferative Phase endometrium explant cultures.
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triphasic pattern in the ex vivo response of human Proliferative Phase endometrium to oestrogens
Faculty of Health, 2004Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, E. Marbaix, Rick Kamps, C. Galant, Annemiek W. Nap, A.f.p.m. De Goeij, A. Ederveen, Patrick G. GroothuisAbstract:The aim of this study was to evaluate the ex vivo oestrogen responsiveness of human Proliferative Phase endometrium using short-term explant cultures. The effects of oestrogen (17beta-E2) on proliferation and the expression of oestrogen-responsive genes known to be involved in regulating endometrial function were evaluated. Three distinct response patterns could be distinguished: (1) the menstrual (M) Phase pattern (cycle days 2-5), which is characterised by a complete lack in the Proliferative response to 17beta-E2, while an increased expression of AR (2.6-fold, P<0.01), PR (2.7-fold, P<0.01) and COX-2 (3.5-fold, P<0.01) at the mRNA level was observed and a similar upregulation was also found for AR, PR and COX-2 at the protein level; (2) the early Proliferative (EP) Phase pattern (cycle days 6-10) with 17beta-E2 enhanced proliferation in the stroma (1.7-fold, P<0.05), whereas the expression of AR, PR and COX-2 were not affected at the mRNA and protein levels and ER-a mRNA and protein levels were significantly reduced by 17beta-E2; (3) the late Proliferative (LP) Phase pattern (cycle days 11-14), which is characterised by a moderate stimulation of proliferation (1.4-fold, P<0.05) and PR mRNA expression (1.7-fold, P<0.01) by 17beta-E2. In conclusion, three distinct response patterns to 17beta-E2 could be identified with respect to proliferation and the expression of known oestrogen-responsive genes in human Proliferative Phase endometrium explant cultures.
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Triphasic pattern in the ex vivo response of human Proliferative Phase endometrium to oestrogens
2004Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, E. Marbaix, Rick Kamps, C. Galant, Annemiek W. Nap, A.f.p.m. De Goeij, A. Ederveen, Patrick G. GroothuisAbstract:The aim of this study was to evaluate the ex vivo oestrogen responsiveness of human Proliferative Phase endometrium using short-term explant cultures. The effects of oestrogen (17beta-E2) on proliferation and the expression of oestrogen-responsive genes known to be involved in regulating endometrial function were evaluated. Three distinct response patterns could be distinguished: (1) the menstrual (M) Phase pattern (cycle days 2-5), which is characterised by a complete lack in the Proliferative response to 17beta-E2, while an increased expression of AR (2.6-fold, P
Chamindie Punyadeera - One of the best experts on this subject based on the ideXlab platform.
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Expression and regulation of vascular endothelial growth factor ligands and receptors during menstruation and post-menstrual repair of human endometrium
Molecular human reproduction, 2006Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, Rick Kamps, A.f.p.m. De Goeij, Victor L. Thijssen, S. Tchaikovski, Bert Delvoux, Arjan W. Griffioen, Patrick G. GroothuisAbstract:Regeneration and growth of the human endometrium after shedding of the functional layer during menstruation depends on an adequate angiogenic response. We analysed the mRNA expression levels of all known vascular endothelial growth factor (VEGF) ligands and receptors in human endometrium collected in the menstrual and Proliferative Phases of the menstrual cycle. In addition, we evaluated the expression of VEGF-A, VEGF-R2 and NRP-1 at the protein level. Two periods of elevated mRNA expression of ligands and receptors were observed, separated by a distinct drop at cycle days (CDs) 9 and 10. Immunohistochemical staining showed that VEGF and VEGF-R2 were expressed in epithelial, stromal and endothelial cells. NRP-1 was mainly confined to stroma and blood vessels; only in late-Proliferative endometrium, epithelial staining was also observed. Except for endothelial VEGF-R2 expression in CDs 6-8, there were no significant differences in the expression of VEGF, VEGF-R2 or NRP-1 in any of the cell compartments. In contrast, VEGF release by cultured human endometrium explants decreased during the Proliferative Phase. This output was significantly reduced in menstrual and early-Proliferative endometrium by estradiol (E2) treatment. Western blot analysis indicated that part of the VEGF-A was trapped in the extracellular matrix (ECM). Changes in VEGF ligands and receptors were associated with elevated expression of the hypoxia markers HIF1alpha and CA-IX in the menstrual and early Proliferative Phases. HIF1alpha was also detected in late-Proliferative Phase endometrium. Our findings indicate that VEGF-A exerts its actions mostly during the first half of the Proliferative Phase. Furthermore, VEGF-A production appears to be triggered by hypoxia in the menstrual Phase and subsequently suppressed by estrogen during the late Proliferative Phase.
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Expression and regulation of vascular endothelial growth factor ligands and receptors during menstruation and post-menstrual repair of human endometrium
2006Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, Rick Kamps, A.f.p.m. De Goeij, Victor L. Thijssen, S. Tchaikovski, Bert Delvoux, Arjan W. Griffioen, Patrick G. GroothuisAbstract:*This article is free to read on the publisher's website* Regeneration and growth of the human endometrium after shedding of the functional layer during menstruation depends on an adequate angiogenic response. We analysed the mRNA expression levels of all known vascular endothelial growth factor (VEGF) ligands and receptors in human endometrium collected in the menstrual and Proliferative Phases of the menstrual cycle. In addition, we evaluated the expression of VEGF-A, VEGF-R2 and NRP-1 at the protein level. Two periods of elevated mRNA expression of ligands and receptors were observed, separated by a distinct drop at cycle days (CDs) 9 and 10. Immunohistochemical staining showed that VEGF and VEGF-R2 were expressed in epithelial, stromal and endothelial cells. NRP-1 was mainly confined to stroma and blood vessels; only in late-Proliferative endometrium, epithelial staining was also observed. Except for endothelial VEGF-R2 expression in CDs 6-8, there were no significant differences in the expression of VEGF, VEGF-R2 or NRP-1 in any of the cell compartments. In contrast, VEGF release by cultured human endometrium explants decreased during the Proliferative Phase. This output was significantly reduced in menstrual and early-Proliferative endometrium by estradiol (E2) treatment. Western blot analysis indicated that part of the VEGF-A was trapped in the extracellular matrix (ECM). Changes in VEGF ligands and receptors were associated with elevated expression of the hypoxia markers HIF1 alpha and CA-IX in the menstrual and early Proliferative Phases. HIF1 alpha was also detected in late-Proliferative Phase endometrium. Our findings indicate that VEGF-A exerts its actions mostly during the first half of the Proliferative Phase. Furthermore, VEGF-A production appears to be triggered by hypoxia in the menstrual Phase and subsequently suppressed toy estrogen during the late Proliferative Phase.
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Triphasic pattern in the ex vivo response of human Proliferative Phase endometrium to oestrogens.
The Journal of steroid biochemistry and molecular biology, 2004Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, E. Marbaix, Rick Kamps, C. Galant, Annemiek W. Nap, A.f.p.m. De Goeij, A. Ederveen, Patrick G. GroothuisAbstract:The aim of this study was to evaluate the ex vivo oestrogen responsiveness of human Proliferative Phase endometrium using short-term explant cultures. The effects of oestrogen (17β-E2) on proliferation and the expression of oestrogen-responsive genes known to be involved in regulating endometrial function were evaluated. Three distinct response patterns could be distinguished: (1) the menstrual (M) Phase pattern (cycle days 2-5), which is characterised by a complete lack in the Proliferative response to 17β-E2, while an increased expression of AR (2.6-fold, P < 0.01), PR (2.7-fold, P < 0.01) and COX-2 (3.5-fold, P < 0.01) at the mRNA level was observed and a similar upregulation was also found for AR, PR and COX-2 at the protein level; (2) the early Proliferative (EP) Phase pattern (cycle days 6-10) with 17β-E2 enhanced proliferation in the stroma (1.7-fold, P < 0.05), whereas the expression of AR, PR and COX-2 were not affected at the mRNA and protein levels and ER-α mRNA and protein levels were significantly reduced by 17β-E2; (3) the late Proliferative (LP) Phase pattern (cycle days 11-14), which is characterised by a moderate stimulation of proliferation (1.4-fold, P < 0.05) and PR mRNA expression (1.7-fold, P < 0.01) by 17β-E2. In conclusion, three distinct response patterns to 17β-E2 could be identified with respect to proliferation and the expression of known oestrogen-responsive genes in human Proliferative Phase endometrium explant cultures.
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triphasic pattern in the ex vivo response of human Proliferative Phase endometrium to oestrogens
Faculty of Health, 2004Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, E. Marbaix, Rick Kamps, C. Galant, Annemiek W. Nap, A.f.p.m. De Goeij, A. Ederveen, Patrick G. GroothuisAbstract:The aim of this study was to evaluate the ex vivo oestrogen responsiveness of human Proliferative Phase endometrium using short-term explant cultures. The effects of oestrogen (17beta-E2) on proliferation and the expression of oestrogen-responsive genes known to be involved in regulating endometrial function were evaluated. Three distinct response patterns could be distinguished: (1) the menstrual (M) Phase pattern (cycle days 2-5), which is characterised by a complete lack in the Proliferative response to 17beta-E2, while an increased expression of AR (2.6-fold, P<0.01), PR (2.7-fold, P<0.01) and COX-2 (3.5-fold, P<0.01) at the mRNA level was observed and a similar upregulation was also found for AR, PR and COX-2 at the protein level; (2) the early Proliferative (EP) Phase pattern (cycle days 6-10) with 17beta-E2 enhanced proliferation in the stroma (1.7-fold, P<0.05), whereas the expression of AR, PR and COX-2 were not affected at the mRNA and protein levels and ER-a mRNA and protein levels were significantly reduced by 17beta-E2; (3) the late Proliferative (LP) Phase pattern (cycle days 11-14), which is characterised by a moderate stimulation of proliferation (1.4-fold, P<0.05) and PR mRNA expression (1.7-fold, P<0.01) by 17beta-E2. In conclusion, three distinct response patterns to 17beta-E2 could be identified with respect to proliferation and the expression of known oestrogen-responsive genes in human Proliferative Phase endometrium explant cultures.
-
Triphasic pattern in the ex vivo response of human Proliferative Phase endometrium to oestrogens
2004Co-Authors: Chamindie Punyadeera, Gerard A.j. Dunselman, E. Marbaix, Rick Kamps, C. Galant, Annemiek W. Nap, A.f.p.m. De Goeij, A. Ederveen, Patrick G. GroothuisAbstract:The aim of this study was to evaluate the ex vivo oestrogen responsiveness of human Proliferative Phase endometrium using short-term explant cultures. The effects of oestrogen (17beta-E2) on proliferation and the expression of oestrogen-responsive genes known to be involved in regulating endometrial function were evaluated. Three distinct response patterns could be distinguished: (1) the menstrual (M) Phase pattern (cycle days 2-5), which is characterised by a complete lack in the Proliferative response to 17beta-E2, while an increased expression of AR (2.6-fold, P
Tracey A. Edgell - One of the best experts on this subject based on the ideXlab platform.
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Idiopathic infertility in women is associated with distinct changes in Proliferative Phase uterine fluid proteins.
Biology of reproduction, 2018Co-Authors: Harriet Fitzgerald, Jemma Evans, Nicholas Johnson, Giuseppe Infusini, Andrew I. Webb, Luk Rombauts, Beverley Vollenhoven, Lois A. Salamonsen, Tracey A. EdgellAbstract:The regenerative, Proliferative Phase of a woman's menstrual cycle is a critical period which lays the foundation for the subsequent, receptive secretory Phase. Although endometrial glands and their secretions are essential for embryo implantation and survival, the Proliferative Phase, when these glands form, has been rarely examined. We hypothesized that alterations in the secreted proteome of the endometrium of idiopathic infertile women would reflect a disturbance in Proliferative Phase endometrial regeneration. Our aim was to compare the proteomic profile of Proliferative Phase uterine fluid from fertile (n = 9) and idiopathic infertile (n = 10) women. Proteins with ≥2-fold change (P
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the Proliferative Phase underpins endometrial development altered cytokine profiles in uterine lavage fluid of women with idiopathic infertility
Cytokine, 2016Co-Authors: Harriet Fitzgerald, Beverley Vollenhoven, Lois A. Salamonsen, Luk J.r. Rombauts, Tracey A. EdgellAbstract:Endometrial gland development occurs during the Proliferative Phase of a woman's menstrual cycle, laying the foundation for the subsequent receptive, secretory Phase when pregnancy is established. Idiopathic infertility has been rarely investigated with respect to the Proliferative Phase endometrium. We investigated whether gland development and/or altered secretion of cytokines during the Proliferative Phase is associated with infertility. Area of the glandular epithelium (GE) was measured in Proliferative Phase endometrial tissue collected from fertile (n=18) and infertile (n=14) women. Cytokines were measured in Proliferative Phase uterine lavage of fertile (n=15) and infertile (n=15) women. Immunohistochemistry determined cellular localisation of transforming growth factor alpha (TGFα) and interferon gamma (IFNγ) in Proliferative Phase endometrial tissue. For statistical analysis the cohort was divided into women <35years and ⩾35years. There were no significant differences in GE area of infertile and fertile women. C-C motif chemokine 11 (P=0.048), TGFα (P=0.049), IFNγ (P=0.033) and interleukin-1 alpha (P=0.047) were significantly elevated in uterine lavage from infertile women <35years compared to fertile but not in women ⩾35years. TGFα and IFNγ localised predominantly to GE in both the fertile and infertile endometrium. The potential impact of this altered Proliferative Phase environment on subsequent receptivity is discussed.
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The Proliferative Phase underpins endometrial development: Altered cytokine profiles in uterine lavage fluid of women with idiopathic infertility.
Cytokine, 2016Co-Authors: Harriet Fitzgerald, Beverley Vollenhoven, Lois A. Salamonsen, Luk J.r. Rombauts, Tracey A. EdgellAbstract:Endometrial gland development occurs during the Proliferative Phase of a woman's menstrual cycle, laying the foundation for the subsequent receptive, secretory Phase when pregnancy is established. Idiopathic infertility has been rarely investigated with respect to the Proliferative Phase endometrium. We investigated whether gland development and/or altered secretion of cytokines during the Proliferative Phase is associated with infertility. Area of the glandular epithelium (GE) was measured in Proliferative Phase endometrial tissue collected from fertile (n=18) and infertile (n=14) women. Cytokines were measured in Proliferative Phase uterine lavage of fertile (n=15) and infertile (n=15) women. Immunohistochemistry determined cellular localisation of transforming growth factor alpha (TGFα) and interferon gamma (IFNγ) in Proliferative Phase endometrial tissue. For statistical analysis the cohort was divided into women
