The Experts below are selected from a list of 1245 Experts worldwide ranked by ideXlab platform
Nazare Souza Bissoli - One of the best experts on this subject based on the ideXlab platform.
-
long term treatment with Nandrolone Decanoate impairs mesenteric vascular relaxation in both sedentary and exercised female rats
Steroids, 2017Co-Authors: Izabela Facco Caliman, Tadeu Uggere De Andrade, Cristian Bernabe, Antonio Ferreira De Melo, Girlândia Alexandre Brasil, Andrews Marques Do Nascimento, Ewelyne Miranda De Lima, Suely G Figueiredo, Nazare Souza BissoliAbstract:Abstract Nandrolone Decanoate (ND) is an Anabolic Androgenic Steroid (AAS) that under abusive regimen can lead to multiple physiological adverse effects. Studies of AAS-mediated cardiovascular (CV) alterations were mostly taken from male subjects, even though women are also susceptible to the effects of AAS and gender-specific differences in susceptibility to vascular diseases exist. Here we investigate ND-induced vascular reactivity alterations in both sedentary and exercised female rats and whether these alterations depend on endothelium-derived factors. We show that chronic exposure of female Wistar rats to ND (20 mg/Kg/week for 4 weeks) impaired the vascular mesenteric bed (MVB) reactivity to vasodilator (acetylcholine) agonist. The endothelium-dependent Nitric Oxide (NO) component was reduced in ND-treated rats, whereas neither the endothelium-derived hyperpolarizing factor (EDHF) component nor prostanoids were altered in the MVBs. Endothelial dysfunction observed in ND-treated rats was associated with decreased eNOS (Ser 1177 ) and Akt (Ser 473 ) phosphorylation sites and upregulation of iNOS and NADPH oxidase expression. Exercise training by weight lifting in water did not improve the vascular alterations induced by ND treatment. ND treatment also significantly reduced the serum levels of estradiol in females, overriding its CV protective effect. These results help uncover the role of ND modulating endothelial function in the setting of CV disease caused by the abuse of AAS in females. If this translates to humans, young women abusing AAS can potentially lose the cardio protective effect rendered by estrogen and be more susceptible to CV alterations.
-
Nandrolone Decanoate induces cardiac and renal remodeling in female rats without modification in physiological parameters the role of anp system
Life Sciences, 2015Co-Authors: Girlândia Alexandre Brasil, Tadeu Uggere De Andrade, Ana Raquel Santos Medeiros, G R Abreu, Izabela Facco Caliman, Andrews Marques Do Nascimento, Ewelyne Miranda De Lima, Mauro Sergio Batista Silva, Adelina M Reis, Nazare Souza BissoliAbstract:ABSTRACT Anabolic-androgenic steroids are misused, including women, but little is known about the cardiovascular effects of these drugs on females. Aim Evaluated the effects of Nandrolone Decanoate (ND), physical exercise and estrogen deficiency on female rats. Main methods Female Wistar rats were divided into 8 groups: S and OVX: (SHAM: sham surgery; OVX: ovariectomy, vehicle), SE and OVXE (resistance exercise 5 times a week, vehicle), SD and OVXD (treated with ND, 20 mg/kg/week for 4 weeks); SDE and OVXDE. Treatments were initiated 21 days after surgery. The Bezold–Jarisch reflex was assessed by Phenylbiguanide administration. The right atrium, kidney, and serum were collected for molecular analyses by RT-PCR of atrial natriuretic peptide (ANP), A-type natriuretic peptide receptor (NPR-A) and NPR-C. ELISA assay to estradiol and testosterone concentrations. The gastrocnemius muscle, heart and kidney weights/tibia length were measured. Morphometric analysis of heart was made (H/E) and collagen content of heart and kidney were evaluated using Pirossirius Red. Key findings ND treatment increased ANP expression on atrium and decreased NPR-A expression in kidney. Physical exercise and ovariectomy did not alter this parameter. NPR-C level was reduced in the SDE and OVXDE. Renal and cardiac hypertrophy was observed after ND treatment, with collagen deposition. Plasma estrogen concentrations were reduced and serum testosterone concentrations were increased after ND treatment. Significance ANP has an important role in modulating the cardiovascular effects of ND in females. This modulating may have occurred by the increasing ANP expression, reducing NPR-A and NPR-C expression levels, and changing sex hormone levels.
-
long term treatment with supraphysiological doses of Nandrolone Decanoate reduces the sensitivity of bezold jarisch reflex control of heart rate and blood pressure
Pharmacological Research, 2009Co-Authors: Nazare Souza Bissoli, Maria Carmen Lopes Ferreira Silva Santos, Vera Cristina W Busato, Ana Raquel Santos Medeiros, G R Abreu, M R Moyses, Robson Dettman Jarske, Tadeu Uggere De AndradeAbstract:We investigated the influence of long-term treatment with supraphysiological doses of an anabolic-androgenic steroid on the Bezold–Jarisch reflex (BJR) control of heart rate (HR) and diastolic arterial pressure (DAP), and whether this treatment induced cardiac hypertrophy. Male rats were treated with Nandrolone Decanoate (ND) (10 mg kg−1 body weight for 8 weeks; DECA) or vehicle (control animals; CON). After 8 weeks of treatment, the BJR was evaluated by bradycardia and hypotension responses that were elicited by serotonin administration (2–32 μg kg−1). Mean arterial pressure (MAP) was assessed and cardiac hypertrophy was determined by the ratio of the left and right ventricle weight/body weight (LVW/BW and RVW/BW, respectively) and by histological analysis. Total body protein (TBP) content was also evaluated. Nandrolone Decanoate treatment increased MAP (CON = 99 ± 1 mmHg; DECA = 109 ± 2 mmHg; p < 0.01) but did not change the mean basal HR (CON = 356 ± 13 bpm; DECA = 367 ± 11 bpm). The treatment also induced LV and RV hypertrophy (LVW/BW: CON = 1.86 ± 0.04 mg g−1, DECA = 2.17 ± 0.04 mg g−1, p < 0.01; RVW/BW: CON = 0.42 ± 0.02 mg g−1, DECA = 0.53 ± 0.03 mg g−1, p < 0.05) and reduced the number of myocyte nuclei/high-power field (CON = 23.0 ± 2; DECA = 9.4 ± 1.0; p < 0.01). ND treatment blunted the HR and DAP decreases induced by serotonin. ND determines an increase in the TBP content in DECA group (35 ± 3%; p < 0.01) compared with control animals (18 ± 1%). We conclude that 8 weeks of ND treatment induces anabolic effect, cardiac hypertrophy and an elevation of MAP. This treatment also reduces the sensitivity of the BJR control of bradycardia and blood pressure, possibly due to cardiac hypertrophy. The blunted BJR response could contribute to the MAP elevation in DECA animals.
-
higher physiological doses of Nandrolone Decanoate do not influence the bezold jarish reflex control of bradycardia
Archives of Medical Research, 2008Co-Authors: Tadeu Uggere De Andrade, Maria Carmen Lopes Ferreira Silva Santos, Vera Cristina W Busato, Ana Raquel Santos Medeiros, G R Abreu, M R Moyses, Nazare Souza BissoliAbstract:Background We investigated the influence of short-term treatment with supraphysiological doses of an anabolic-androgenic steroid on the Bezold-Jarisch reflex (BJR) control of heart rate (HR) and whether this treatment induced cardiac hypertrophy and anabolic effects in rats. Methods Male rats were treated with Nandrolone Decanoate (10 mg/kg −1 body weight/4 weeks; DECA) or vehicle control (CON). After 4 weeks of treatment, BJR was evaluated by bradycardia responses that were elicited by serotonin administration (2–32 μg/kg −1 ). Mean arterial pressure (MAP) was assessed and cardiac hypertrophy was determined by the left ventricle weight/body weight (LVW/BW) ratio. Histological analyses of LV and the measurement of the total body protein content of the animals were performed. Results Nandrolone Decanoate (ND) treatment had no effect on the MAP (CON = 105 ± 5; DECA = 110 ± 3 mmHg). However, the mean basal HR of DECA animals was significantly lower than that of control animals (CON = 381 ± 14; DECA = 324 ± 12 bpm; p p p Conclusions We concluded that 4 weeks ND treatment induced an anabolic effect and the beginnings of LV remodeling, mainly due to excessive collagen deposition in the cardiac extracellular matrix. However, the treatment did not influence BJR control of bradycardia, an effect that could be explained by an enhanced efferent vagal tonus in DECA animals.
Tadeu Uggere De Andrade - One of the best experts on this subject based on the ideXlab platform.
-
long term treatment with Nandrolone Decanoate impairs mesenteric vascular relaxation in both sedentary and exercised female rats
Steroids, 2017Co-Authors: Izabela Facco Caliman, Tadeu Uggere De Andrade, Cristian Bernabe, Antonio Ferreira De Melo, Girlândia Alexandre Brasil, Andrews Marques Do Nascimento, Ewelyne Miranda De Lima, Suely G Figueiredo, Nazare Souza BissoliAbstract:Abstract Nandrolone Decanoate (ND) is an Anabolic Androgenic Steroid (AAS) that under abusive regimen can lead to multiple physiological adverse effects. Studies of AAS-mediated cardiovascular (CV) alterations were mostly taken from male subjects, even though women are also susceptible to the effects of AAS and gender-specific differences in susceptibility to vascular diseases exist. Here we investigate ND-induced vascular reactivity alterations in both sedentary and exercised female rats and whether these alterations depend on endothelium-derived factors. We show that chronic exposure of female Wistar rats to ND (20 mg/Kg/week for 4 weeks) impaired the vascular mesenteric bed (MVB) reactivity to vasodilator (acetylcholine) agonist. The endothelium-dependent Nitric Oxide (NO) component was reduced in ND-treated rats, whereas neither the endothelium-derived hyperpolarizing factor (EDHF) component nor prostanoids were altered in the MVBs. Endothelial dysfunction observed in ND-treated rats was associated with decreased eNOS (Ser 1177 ) and Akt (Ser 473 ) phosphorylation sites and upregulation of iNOS and NADPH oxidase expression. Exercise training by weight lifting in water did not improve the vascular alterations induced by ND treatment. ND treatment also significantly reduced the serum levels of estradiol in females, overriding its CV protective effect. These results help uncover the role of ND modulating endothelial function in the setting of CV disease caused by the abuse of AAS in females. If this translates to humans, young women abusing AAS can potentially lose the cardio protective effect rendered by estrogen and be more susceptible to CV alterations.
-
Nandrolone Decanoate induces cardiac and renal remodeling in female rats without modification in physiological parameters the role of anp system
Life Sciences, 2015Co-Authors: Girlândia Alexandre Brasil, Tadeu Uggere De Andrade, Ana Raquel Santos Medeiros, G R Abreu, Izabela Facco Caliman, Andrews Marques Do Nascimento, Ewelyne Miranda De Lima, Mauro Sergio Batista Silva, Adelina M Reis, Nazare Souza BissoliAbstract:ABSTRACT Anabolic-androgenic steroids are misused, including women, but little is known about the cardiovascular effects of these drugs on females. Aim Evaluated the effects of Nandrolone Decanoate (ND), physical exercise and estrogen deficiency on female rats. Main methods Female Wistar rats were divided into 8 groups: S and OVX: (SHAM: sham surgery; OVX: ovariectomy, vehicle), SE and OVXE (resistance exercise 5 times a week, vehicle), SD and OVXD (treated with ND, 20 mg/kg/week for 4 weeks); SDE and OVXDE. Treatments were initiated 21 days after surgery. The Bezold–Jarisch reflex was assessed by Phenylbiguanide administration. The right atrium, kidney, and serum were collected for molecular analyses by RT-PCR of atrial natriuretic peptide (ANP), A-type natriuretic peptide receptor (NPR-A) and NPR-C. ELISA assay to estradiol and testosterone concentrations. The gastrocnemius muscle, heart and kidney weights/tibia length were measured. Morphometric analysis of heart was made (H/E) and collagen content of heart and kidney were evaluated using Pirossirius Red. Key findings ND treatment increased ANP expression on atrium and decreased NPR-A expression in kidney. Physical exercise and ovariectomy did not alter this parameter. NPR-C level was reduced in the SDE and OVXDE. Renal and cardiac hypertrophy was observed after ND treatment, with collagen deposition. Plasma estrogen concentrations were reduced and serum testosterone concentrations were increased after ND treatment. Significance ANP has an important role in modulating the cardiovascular effects of ND in females. This modulating may have occurred by the increasing ANP expression, reducing NPR-A and NPR-C expression levels, and changing sex hormone levels.
-
long term treatment with supraphysiological doses of Nandrolone Decanoate reduces the sensitivity of bezold jarisch reflex control of heart rate and blood pressure
Pharmacological Research, 2009Co-Authors: Nazare Souza Bissoli, Maria Carmen Lopes Ferreira Silva Santos, Vera Cristina W Busato, Ana Raquel Santos Medeiros, G R Abreu, M R Moyses, Robson Dettman Jarske, Tadeu Uggere De AndradeAbstract:We investigated the influence of long-term treatment with supraphysiological doses of an anabolic-androgenic steroid on the Bezold–Jarisch reflex (BJR) control of heart rate (HR) and diastolic arterial pressure (DAP), and whether this treatment induced cardiac hypertrophy. Male rats were treated with Nandrolone Decanoate (ND) (10 mg kg−1 body weight for 8 weeks; DECA) or vehicle (control animals; CON). After 8 weeks of treatment, the BJR was evaluated by bradycardia and hypotension responses that were elicited by serotonin administration (2–32 μg kg−1). Mean arterial pressure (MAP) was assessed and cardiac hypertrophy was determined by the ratio of the left and right ventricle weight/body weight (LVW/BW and RVW/BW, respectively) and by histological analysis. Total body protein (TBP) content was also evaluated. Nandrolone Decanoate treatment increased MAP (CON = 99 ± 1 mmHg; DECA = 109 ± 2 mmHg; p < 0.01) but did not change the mean basal HR (CON = 356 ± 13 bpm; DECA = 367 ± 11 bpm). The treatment also induced LV and RV hypertrophy (LVW/BW: CON = 1.86 ± 0.04 mg g−1, DECA = 2.17 ± 0.04 mg g−1, p < 0.01; RVW/BW: CON = 0.42 ± 0.02 mg g−1, DECA = 0.53 ± 0.03 mg g−1, p < 0.05) and reduced the number of myocyte nuclei/high-power field (CON = 23.0 ± 2; DECA = 9.4 ± 1.0; p < 0.01). ND treatment blunted the HR and DAP decreases induced by serotonin. ND determines an increase in the TBP content in DECA group (35 ± 3%; p < 0.01) compared with control animals (18 ± 1%). We conclude that 8 weeks of ND treatment induces anabolic effect, cardiac hypertrophy and an elevation of MAP. This treatment also reduces the sensitivity of the BJR control of bradycardia and blood pressure, possibly due to cardiac hypertrophy. The blunted BJR response could contribute to the MAP elevation in DECA animals.
-
higher physiological doses of Nandrolone Decanoate do not influence the bezold jarish reflex control of bradycardia
Archives of Medical Research, 2008Co-Authors: Tadeu Uggere De Andrade, Maria Carmen Lopes Ferreira Silva Santos, Vera Cristina W Busato, Ana Raquel Santos Medeiros, G R Abreu, M R Moyses, Nazare Souza BissoliAbstract:Background We investigated the influence of short-term treatment with supraphysiological doses of an anabolic-androgenic steroid on the Bezold-Jarisch reflex (BJR) control of heart rate (HR) and whether this treatment induced cardiac hypertrophy and anabolic effects in rats. Methods Male rats were treated with Nandrolone Decanoate (10 mg/kg −1 body weight/4 weeks; DECA) or vehicle control (CON). After 4 weeks of treatment, BJR was evaluated by bradycardia responses that were elicited by serotonin administration (2–32 μg/kg −1 ). Mean arterial pressure (MAP) was assessed and cardiac hypertrophy was determined by the left ventricle weight/body weight (LVW/BW) ratio. Histological analyses of LV and the measurement of the total body protein content of the animals were performed. Results Nandrolone Decanoate (ND) treatment had no effect on the MAP (CON = 105 ± 5; DECA = 110 ± 3 mmHg). However, the mean basal HR of DECA animals was significantly lower than that of control animals (CON = 381 ± 14; DECA = 324 ± 12 bpm; p p p Conclusions We concluded that 4 weeks ND treatment induced an anabolic effect and the beginnings of LV remodeling, mainly due to excessive collagen deposition in the cardiac extracellular matrix. However, the treatment did not influence BJR control of bradycardia, an effect that could be explained by an enhanced efferent vagal tonus in DECA animals.
Fred Nyberg - One of the best experts on this subject based on the ideXlab platform.
-
increased dopamine transporter density in the male rat brain following chronic Nandrolone Decanoate administration
Neuroscience Letters, 2004Co-Authors: Anna M S Kindlundh, Sadia Rahman, Jonas Lindblom, Fred NybergAbstract:Adolescent males currently employ anabolic-androgenic steroids (AAS) to become intoxicated, besides the traditional desires of an improved physical appearance and enhanced sports performance. Several studies indicate that AAS affect the brain reward system. Recently chronic administration with Nandrolone Decanoate to male rats has been shown to increase the dopamine transporter (DAT) density in the striatum visualised in vivo by positron emission tomography. The present study aimed to investigate if the increased DAT density could be confirmed using in vitro autoradiography following a comparable regimen of Nandrolone treatment. Specific binding of 50 pM [125I] RTI-55 in the presence of 1 microM citalopram was used to label DAT. Two weeks of Nandrolone Decanoate administration at the supra-therapeutic doses 1, 5 and 15 mg/kg per day increased DAT density in the caudate putamen at all three doses. In conclusion, this study confirms that chronic Nandrolone administration increases dopamine transporter density in the CPU and therefore supports the theory that AAS affects the dopamine system in the male rat brain.
-
the anabolic androgenic steroid Nandrolone Decanoate affects the density of dopamine receptors in the male rat brain
European Journal of Neuroscience, 2001Co-Authors: Anna M S Kindlundh, Jonas Lindblom, Lena Bergstrom, Jarl E S Wikberg, Fred NybergAbstract:In recent years a male group of anabolic-androgenic steroid misusers has been identified to share socio-demographic and personality related background factors with misusers of psychotropic substances, as well as being involved in habits of multiple drug use. The present study aimed to assess whether anabolic-androgenic steroids (AAS) would affect the density of the dopamine receptors in areas implicated in reward and behaviour in the male rat brain. The effects of 2 weeks of treatment with i.m. injections of Nandrolone Decanoate (15 mg/kg/day) on the expression of the D(1)-like and D(2)-like receptors were evaluated by autoradiography. Specific binding of D(1)-like receptors was significantly down regulated in the caudate putamen, the nucleus accumbens core and shell. D(2)-like receptor densities were down regulated in the nucleus accumbens shell, but up regulated in the caudate putamen, the nucleus accumbens core and the ventral tegmental area. These results are compatible with Nandrolone induced neuroadaptive alterations in dopamine circuits associated with motor functions and behavioural paradigms known to be affected following AAS misuse.
Fakhrodin Mesbah Ardekani - One of the best experts on this subject based on the ideXlab platform.
-
the reversibility of sperm quality after discontinuing Nandrolone Decanoate in adult male rats
Asian Journal of Andrology, 2007Co-Authors: Saied Karbalaydoust, Ali Noorafshan, Fakhrodin Mesbah Ardekani, Hossien MirkhaniAbstract:Aim: To investigate the reversibility of the effect of Nandrolone Decanoate (ND) on sperm parameters after discontinuing the drug. Methods: Three groups of rats received peanut oil (control), low and high doses of ND for 14 weeks, respectively. Each group was divided into subgroups A and B, in which rats were killed 14 and 28 weeks after initiating the injection, respectively. Results: Sperm count and motile sperm fraction were decreased in the subgroups A and B that received low and high doses of ND in comparison with the controls A and B, respectively. The sperm count and motile sperm fraction increased in the subgroups B that received low and high doses of ND in comparison with their corresponding subgroups A. The number of normal morphology sperm was decreased significantly in subgroups A receiving low and high doses of ND in comparison with the control subgroup A. However, this parameter was not decreased in subgroups B receiving low and high doses in comparison with the control subgroup B. The normal sperm morphology did not show any significant differences in the subgroups B in comparison with their corresponding subgroups A. Conclusion: The 14-week injection of low and high doses of ND decreases sperm quality and quantity in rats. These parameters were improved after discontinuing ND, but not recovered completely even when they are left untreated for 14 weeks.
-
high doses of Nandrolone Decanoate reduce volume of testis and length of seminiferous tubules in rats
Apmis, 2005Co-Authors: Ali Noorafshan, Saied Karbalaydoust, Fakhrodin Mesbah ArdekaniAbstract:Anabolic-androgenic steroid (AAS) compounds rank among the drugs most widely abused with the goal of improving athletic ability, appearance, or muscle mass. It has been shown that these compounds have adverse effects on human and animal physiology and sperm quality, but quantitative structural changes of the testis have received less attention. The present study was conducted to evaluate the effects of Nandrolone Decanoate, which is one of the AAS compounds, on testis weight and volume, diameter and length of seminiferous tubules in rats by unbiased stereological methods. Adult rats were divided into three groups. The first comprised control rats; the second and third groups received low and high doses of Nandrolone Decanoate for 14 weeks. The rats were then left untreated for 14 weeks. After removal of the testis, stereological study of these tissues showed that the mean volume of testis and length of the seminiferous tubules in the animals that received high doses of Nandrolone Decanoate were reduced approximately 32% (p<0.01) and approximately 31% (p<0.04), respectively, in comparison with the control group. It can be concluded that the high doses of Nandrolone Decanoate produce structural changes in the rat testis that remain 14 weeks after stopping injection of the drug.
Ali Noorafshan - One of the best experts on this subject based on the ideXlab platform.
-
stereological estimation of ovarian oocyte volume surface area and number application on mice treated with Nandrolone Decanoate
Folia Histochemica Et Cytobiologica, 2012Co-Authors: Saied Karbalaydoust, Ali NoorafshanAbstract:Changes in the number and size of oocytes can lead to fertilization problems. The present study aimed to evaluate the number, volume, and surface area of oocytes in healthy as well as Nandrolone Decanoate-treated (ND) mice using stereological methods. Five control mice received vehicle, and five ND-treated mice received ND. Using the 'isotropic Cavalieri' design', the ovary was sectioned. The volume of the ovary (cortex and medulla) was estimated. The oocytes' volume and surface area were estimated using the invariator. The number of the oocytes was estimated using an optical disector. The volumes of the ovary, cortex, and medulla decreased ~50% in the ND-treated mice. The mean number (coefficient of variation) of preantral, antral, and atretic oocytes in the control ovary were 1,690 (0.29), 2,100 (0.52), and 3,900 (0.2), respectively, which decreased ~54%, ~87%, and ~91%, respectively in the ND-treated animals. The mean volume (coefficient of variation) of the preantral, antral, and atretic oocytes were 86,000 (0.27), 110,000 (0.48), and 27,000 (0.33) μm³, respectively. The mean surface area (coefficient of variation) of the three types of oocytes were 9,000 (0.24), 9,900 (0.28), and 4,700 (0.21) μm², respectively. These parameters remained unchanged in the ND-treated mice. ND induces reduction in the number of oocytes, but not in the volume or the surface area.
-
Nandrolone Decanoate increases the volume but not the length of the proximal and distal convoluted tubules of the mouse kidney.
Micron (Oxford England : 1993), 2008Co-Authors: Leila Hoseini, Jamshid Roozbeh, Mehdi Sagheb, Saied Karbalay-doust, Ali NoorafshanAbstract:Abstract Anabolic androgenic steroids are widely used by athletes for increasing their muscle mass. These drugs are also used by some patients with chronic renal disease. But the effect of these drugs on the renal structure has received less attention. To investigate which parts of the kidney are affected by these drugs, mice kidneys were studied stereologically after injection of Nandrolone Decanoate (ND), an anabolic androgenic steroid. The treated group received Nandrolone Decanoate intraperitoneally (solved in olive oil) in doses of 3 mg/kg of body weight and administered in one, two and three doses, respectively, in the first, second and third week of treatment. The mice in the control group received an olive oil solution. One week after the last injection, the mice were anaesthetized and their kidney removed. The analysis of data revealed that the weight of kidney was increased ∼30% (p ≤ 0.006) and its volume increased ∼25% (p ≤ 0.02) in ND treated mice in comparison with the control group. The volume of the cortex increased in ND treated animals ∼44% (p ≤ 0.006). Proximal convoluted tubules (PCT) and distal convoluted tubules (DCT) volume increased ∼25% (p ≤ 0.02) and ∼68% (p ≤ 0.02) in ND treated mice. The volume of glomeruli, other ducts, connective tissues, vessels and the length of PCT, DCT, collecting and Henle’s ducts and vessels did not show significant differences. Conclusion: ND can increase the volume of the renal cortex and its two main parts, i.e. PCT and DCT in mice.
-
the reversibility of sperm quality after discontinuing Nandrolone Decanoate in adult male rats
Asian Journal of Andrology, 2007Co-Authors: Saied Karbalaydoust, Ali Noorafshan, Fakhrodin Mesbah Ardekani, Hossien MirkhaniAbstract:Aim: To investigate the reversibility of the effect of Nandrolone Decanoate (ND) on sperm parameters after discontinuing the drug. Methods: Three groups of rats received peanut oil (control), low and high doses of ND for 14 weeks, respectively. Each group was divided into subgroups A and B, in which rats were killed 14 and 28 weeks after initiating the injection, respectively. Results: Sperm count and motile sperm fraction were decreased in the subgroups A and B that received low and high doses of ND in comparison with the controls A and B, respectively. The sperm count and motile sperm fraction increased in the subgroups B that received low and high doses of ND in comparison with their corresponding subgroups A. The number of normal morphology sperm was decreased significantly in subgroups A receiving low and high doses of ND in comparison with the control subgroup A. However, this parameter was not decreased in subgroups B receiving low and high doses in comparison with the control subgroup B. The normal sperm morphology did not show any significant differences in the subgroups B in comparison with their corresponding subgroups A. Conclusion: The 14-week injection of low and high doses of ND decreases sperm quality and quantity in rats. These parameters were improved after discontinuing ND, but not recovered completely even when they are left untreated for 14 weeks.
-
high doses of Nandrolone Decanoate reduce volume of testis and length of seminiferous tubules in rats
Apmis, 2005Co-Authors: Ali Noorafshan, Saied Karbalaydoust, Fakhrodin Mesbah ArdekaniAbstract:Anabolic-androgenic steroid (AAS) compounds rank among the drugs most widely abused with the goal of improving athletic ability, appearance, or muscle mass. It has been shown that these compounds have adverse effects on human and animal physiology and sperm quality, but quantitative structural changes of the testis have received less attention. The present study was conducted to evaluate the effects of Nandrolone Decanoate, which is one of the AAS compounds, on testis weight and volume, diameter and length of seminiferous tubules in rats by unbiased stereological methods. Adult rats were divided into three groups. The first comprised control rats; the second and third groups received low and high doses of Nandrolone Decanoate for 14 weeks. The rats were then left untreated for 14 weeks. After removal of the testis, stereological study of these tissues showed that the mean volume of testis and length of the seminiferous tubules in the animals that received high doses of Nandrolone Decanoate were reduced approximately 32% (p<0.01) and approximately 31% (p<0.04), respectively, in comparison with the control group. It can be concluded that the high doses of Nandrolone Decanoate produce structural changes in the rat testis that remain 14 weeks after stopping injection of the drug.
