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Zhiliang Jiang - One of the best experts on this subject based on the ideXlab platform.
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An Aptamer-Nanogold Probe for Trace PDGF Using Resonance Rayleigh Scattering as Detection Technique
Advanced Materials Research, 2013Co-Authors: Jin Qiao Long, Aihui Liang, Zhiliang JiangAbstract:The aptamer of platelet-derived growth factor (PDGF) was used to modified Nanogold to obtain a stable aptamer-Nanogold(Apt-NG) probe for PDGF. In the condition of pH 7.2 Na2HPO4-NaH2PO4 buffer solution and 5.33 mmol/L NaCl, the Apt-NG specifically combined with PDGF to form Apt-NG-PDGFA cluster that resulted in the resonance Rayleigh scattering (RRS) intensity at 550 nm. When the PDGF concentration increased, the Nanogold aggregated (NGA) increased, and the RRS intensity at 550 nm increased. The increased RRS intensity ΔI550nm was linear to the PDGF concentration in the range of 3.33-40 ng/mL, with a regression equation of ΔI550nm=10.8 C-24.2, coefficient of 0.9932 and a detection limit of 0.53 ng/mL
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Resonance scattering spectral detection of trace K+ by aptamer-modified Nanogold probe
Spectroscopy and Spectral Analysis, 2010Co-Authors: Tingsheng Li, Aihui Liang, Zhiliang JiangAbstract:: In pH 7.0 Na2HPO4-NaH2PO4 buffer solution, Nanogold particles interacted with the aptamer to form a stable aptamer-Nanogold complex that was not aggregation by NaCl. At 80 degrees C, K+ and aptamer folded to form a stable G-quadruplex that released Nanogold particles, the uncombined Nanogold particles aggregated to large Nanogold clusters that caused the increase in resonance scattering (RS) intensity at 563 nm in high concentration of NaCl, and the laser scattering showed that the average diameter was 120 nm. In the present paper, the resonance scattering spectral characteristics of K+ -ssDNA1-Au, K+ -ssDNA2-Au and K+ -aptamer-Au systems were investigated, and the structural changes of aptamer were studied by circular dichroism spectral technology. Effects of pH value, NaCl concentration, Nanogold concentration, aptamer concentration, and the reactation temperature and time on the resonance scattering intensity were considered in detail. The influence of coexistent substances on the determination of K+ was investigated, result showed that the common heavy metal ions such as Cu2+, Mg2+, Pb2+, Ca2+, Al3+, Zn2+ and Fe3+ do not interfere with the determination, and the method has good selectivity. Under the conditions selected, a 0. 67-3 350 micromol x L(-1) K+ can be detected by the aptamer-Nanogold RS assay, with a detection limit of 0.3 micromol x L(-1) K+, regression equation deltaI = 0.167c-0.7, and a coefficient of 0.9932. The method was used for analysis of K+ in serum sample with the results consistent with the ion-selective electrode method.
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a simple and rapid resonance scattering spectral method for detection of trace hg2 using aptamer Nanogold as probe
Plasmonics, 2010Co-Authors: Aihui Liang, Zhiliang Jiang, Caina JiangAbstract:Single-strand deoxyribonucleic acid (ssDNA) were used to modified Nanogold particle to obtain a aptamer-Nanogold probe (NGssDNA) for Hg(II). The probe is not aggregated in high concentration of NaCl. In the pH 7.0 Na2HPO4-NaH2PO4 buffer solution and in the presence of high concentration of NaCl, NGssDNA interact with Hg(II) to form stable double-strand T-Hg(II)-T mismatches and to release Nanogold particles from the probe. The released Nanogold particles aggregated to form bigger clusters which leaded the resonance scattering (RS) intensity at 540 nm enhanced linearly with the concentration of Hg2+ in the range of 0.39–1666.7 nM, with detection of 0.1 nM. This simple, rapid, and sensitive aptamer-Nanogold RS assay was applied to determination of Hg2+ in wastewater, with satisfactory results.
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resonance scattering spectral detection of trace hg2 using aptamer modified Nanogold as probe and nanocatalyst
Analytical Chemistry, 2009Co-Authors: Zhiliang Jiang, Aihui Liang, Menglin Chen, Xianjiu Liao, Xingcan Shen, Xingcun He, Hesheng JiangAbstract:Single-strand DNA (ssDNA) was used to modify 10 nm Nanogold to obtain an aptamer-modified Nanogold resonance scattering (RS) probe (AussDNA) for detection of Hg(2+). In the presence of NaCl, Hg(2+) interacts with AussDNA to form very stable double-strand T-Hg(2+)-T mismatches and release Nanogold particles that aggregate to large Nanogold clusters causing the RS intensity at 540 nm to be enhanced linearly. On those grounds, 1.3-1667 nM Hg(2+) can be detected rapidly by the aptamer-modified Nanogold RS assay, with a detection limit of 0.7 nM Hg(2+). If the large Nanogold clusters were removed by membrane filtration, the excess AussDNA in the filtrate solution exhibits a catalytic effect on the new Cu(2)O particle reaction between NH(2)OH and Cu(2+)-EDTA complex at 60 degrees C. The excess AussDNA decreased with the addition of Hg(2+), which led the Cu(2)O particle RS intensity at 602 nm to decrease. The decreased RS intensity (DeltaI(602nm)) had a linear response to Hg(2+) concentration in the range of 0.1-400 nM, with a detection limit of 0.03 nM Hg(2+). This aptamer-modified Nanogold catalytic RS method was applied for the detection of Hg(2+) in water samples, with sensitivity, selectivity, and simplicity.
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catalytic effect of Nanogold on cu ii n2h4 reaction and its application to resonance scattering immunoassay
Analytical Chemistry, 2008Co-Authors: Zhiliang Jiang, Aihui Liang, Xianjiu Liao, Anping Deng, Jishun Li, Jianfu Li, Sumei Wang, Yujuan HuangAbstract:In the medium of EDTA−NaOH, Nanogold strongly catalyzed the slow reaction between hydrazine (N2H4) and Cu(II) to form Cu particles, which exhibited a strong resonance scattering (RS) peak at 602 nm. The increased RS intensity at 602 nm (ΔIRS) was linear to the Nanogold concentration in the range of 0.008−2.64 nM, with a detection limit of 1.0 pM Au. The rate equation obtained by the initial rate procedure was VCu = KCu[CCu(II)]2COH1CAu1CN2H41, with an apparent activation energy of 38 kJ.mol−1, and the catalytic reaction mechanism was also discussed. An immunoNanogold-catalytic resonance scattering spectral (RSS) assay was established for detection of microalbumin (Malb), using 10 nm Nanogold to label goat antihuman Malb to obtain an immunoNanogold probe (AuMalb) for Malb. In pH 5.0 citric acid−Na2HPO4 buffer solution, the AuMalb aggregated nonspecifically. Upon addition of Malb, it reacted with the probe to form dispersive AuMalb−Malb immunocomplex in the solution. After centrifugation, the supernatant co...
Aihui Liang - One of the best experts on this subject based on the ideXlab platform.
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An Aptamer-Nanogold Probe for Trace PDGF Using Resonance Rayleigh Scattering as Detection Technique
Advanced Materials Research, 2013Co-Authors: Jin Qiao Long, Aihui Liang, Zhiliang JiangAbstract:The aptamer of platelet-derived growth factor (PDGF) was used to modified Nanogold to obtain a stable aptamer-Nanogold(Apt-NG) probe for PDGF. In the condition of pH 7.2 Na2HPO4-NaH2PO4 buffer solution and 5.33 mmol/L NaCl, the Apt-NG specifically combined with PDGF to form Apt-NG-PDGFA cluster that resulted in the resonance Rayleigh scattering (RRS) intensity at 550 nm. When the PDGF concentration increased, the Nanogold aggregated (NGA) increased, and the RRS intensity at 550 nm increased. The increased RRS intensity ΔI550nm was linear to the PDGF concentration in the range of 3.33-40 ng/mL, with a regression equation of ΔI550nm=10.8 C-24.2, coefficient of 0.9932 and a detection limit of 0.53 ng/mL
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Resonance scattering spectral detection of trace K+ by aptamer-modified Nanogold probe
Spectroscopy and Spectral Analysis, 2010Co-Authors: Tingsheng Li, Aihui Liang, Zhiliang JiangAbstract:: In pH 7.0 Na2HPO4-NaH2PO4 buffer solution, Nanogold particles interacted with the aptamer to form a stable aptamer-Nanogold complex that was not aggregation by NaCl. At 80 degrees C, K+ and aptamer folded to form a stable G-quadruplex that released Nanogold particles, the uncombined Nanogold particles aggregated to large Nanogold clusters that caused the increase in resonance scattering (RS) intensity at 563 nm in high concentration of NaCl, and the laser scattering showed that the average diameter was 120 nm. In the present paper, the resonance scattering spectral characteristics of K+ -ssDNA1-Au, K+ -ssDNA2-Au and K+ -aptamer-Au systems were investigated, and the structural changes of aptamer were studied by circular dichroism spectral technology. Effects of pH value, NaCl concentration, Nanogold concentration, aptamer concentration, and the reactation temperature and time on the resonance scattering intensity were considered in detail. The influence of coexistent substances on the determination of K+ was investigated, result showed that the common heavy metal ions such as Cu2+, Mg2+, Pb2+, Ca2+, Al3+, Zn2+ and Fe3+ do not interfere with the determination, and the method has good selectivity. Under the conditions selected, a 0. 67-3 350 micromol x L(-1) K+ can be detected by the aptamer-Nanogold RS assay, with a detection limit of 0.3 micromol x L(-1) K+, regression equation deltaI = 0.167c-0.7, and a coefficient of 0.9932. The method was used for analysis of K+ in serum sample with the results consistent with the ion-selective electrode method.
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a simple and rapid resonance scattering spectral method for detection of trace hg2 using aptamer Nanogold as probe
Plasmonics, 2010Co-Authors: Aihui Liang, Zhiliang Jiang, Caina JiangAbstract:Single-strand deoxyribonucleic acid (ssDNA) were used to modified Nanogold particle to obtain a aptamer-Nanogold probe (NGssDNA) for Hg(II). The probe is not aggregated in high concentration of NaCl. In the pH 7.0 Na2HPO4-NaH2PO4 buffer solution and in the presence of high concentration of NaCl, NGssDNA interact with Hg(II) to form stable double-strand T-Hg(II)-T mismatches and to release Nanogold particles from the probe. The released Nanogold particles aggregated to form bigger clusters which leaded the resonance scattering (RS) intensity at 540 nm enhanced linearly with the concentration of Hg2+ in the range of 0.39–1666.7 nM, with detection of 0.1 nM. This simple, rapid, and sensitive aptamer-Nanogold RS assay was applied to determination of Hg2+ in wastewater, with satisfactory results.
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resonance scattering spectral detection of trace hg2 using aptamer modified Nanogold as probe and nanocatalyst
Analytical Chemistry, 2009Co-Authors: Zhiliang Jiang, Aihui Liang, Menglin Chen, Xianjiu Liao, Xingcan Shen, Xingcun He, Hesheng JiangAbstract:Single-strand DNA (ssDNA) was used to modify 10 nm Nanogold to obtain an aptamer-modified Nanogold resonance scattering (RS) probe (AussDNA) for detection of Hg(2+). In the presence of NaCl, Hg(2+) interacts with AussDNA to form very stable double-strand T-Hg(2+)-T mismatches and release Nanogold particles that aggregate to large Nanogold clusters causing the RS intensity at 540 nm to be enhanced linearly. On those grounds, 1.3-1667 nM Hg(2+) can be detected rapidly by the aptamer-modified Nanogold RS assay, with a detection limit of 0.7 nM Hg(2+). If the large Nanogold clusters were removed by membrane filtration, the excess AussDNA in the filtrate solution exhibits a catalytic effect on the new Cu(2)O particle reaction between NH(2)OH and Cu(2+)-EDTA complex at 60 degrees C. The excess AussDNA decreased with the addition of Hg(2+), which led the Cu(2)O particle RS intensity at 602 nm to decrease. The decreased RS intensity (DeltaI(602nm)) had a linear response to Hg(2+) concentration in the range of 0.1-400 nM, with a detection limit of 0.03 nM Hg(2+). This aptamer-modified Nanogold catalytic RS method was applied for the detection of Hg(2+) in water samples, with sensitivity, selectivity, and simplicity.
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catalytic effect of Nanogold on cu ii n2h4 reaction and its application to resonance scattering immunoassay
Analytical Chemistry, 2008Co-Authors: Zhiliang Jiang, Aihui Liang, Xianjiu Liao, Anping Deng, Jishun Li, Jianfu Li, Sumei Wang, Yujuan HuangAbstract:In the medium of EDTA−NaOH, Nanogold strongly catalyzed the slow reaction between hydrazine (N2H4) and Cu(II) to form Cu particles, which exhibited a strong resonance scattering (RS) peak at 602 nm. The increased RS intensity at 602 nm (ΔIRS) was linear to the Nanogold concentration in the range of 0.008−2.64 nM, with a detection limit of 1.0 pM Au. The rate equation obtained by the initial rate procedure was VCu = KCu[CCu(II)]2COH1CAu1CN2H41, with an apparent activation energy of 38 kJ.mol−1, and the catalytic reaction mechanism was also discussed. An immunoNanogold-catalytic resonance scattering spectral (RSS) assay was established for detection of microalbumin (Malb), using 10 nm Nanogold to label goat antihuman Malb to obtain an immunoNanogold probe (AuMalb) for Malb. In pH 5.0 citric acid−Na2HPO4 buffer solution, the AuMalb aggregated nonspecifically. Upon addition of Malb, it reacted with the probe to form dispersive AuMalb−Malb immunocomplex in the solution. After centrifugation, the supernatant co...
Alessandro Moscatelli - One of the best experts on this subject based on the ideXlab platform.
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Inhibition of actin polymerisation by low concentration Latrunculin B affects endocytosis and alters exocytosis in shank and tip of tobacco pollen tubes
Plant Biology, 2012Co-Authors: Alessandro Moscatelli, A. I. Idilli, Simona Rodighiero, Marco CaccianigaAbstract:Pollen tube growth depends on the integrity of the actin cytoskeleton that regulates cytoplasmic streaming and secretion. To clarify whether actin also plays a role in pollen tube endocytosis, Latrunculin B (LatB) was employed in internalisation experiments with tobacco pollen tubes, using the lipophilic dye FM4-64 and charged Nanogold. Time-lapse analysis and dissection of endocytosis allowed us to identify internalisation pathways with different sensitivity to LatB. Co-localisation experiments and ultrastructural observations using positively charged Nanogold revealed that LatB significantly inhibited endocytosis in the pollen tube shank, affecting internalisation of the plasma membrane (PM) recycled for secretion, as well as that conveyed to vacuoles. In contrast, endocytosis of negatively charged Nanogold in the tip, which is also conveyed to vacuoles, was not influenced. Experiments of fluorescence recovery after photobleaching (FRAP) of the apical and subapical PM revealed domains with different rates of fluorescence recovery and showed that these differences depend on the actin cytoskeleton integrity. These results show the presence of distinct degradation pathways by demonstrating that actin-dependent and actin-indepedent endocytosis both operate in pollen tubes, internalising tracts of PM to be recycled and broken down. Intriguingly, although most studies concentrate on exocytosis and distension in the apex, the present paper shows that uncharacterised, actin-dependent secretory activity occurs in the shank of pollen tubes.
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distinct endocytic pathways identified in tobacco pollen tubes using charged Nanogold
Journal of Cell Science, 2007Co-Authors: Alessandro Moscatelli, Simona Rodighiero, F Ciampolini, Elisabetta Onelli, M Cresti, Nadia Santo, Aurora Irene IdilliAbstract:In an attempt to dissect endocytosis in Nicotiana tabacum L. pollen tubes, two different probes – positively or negatively charged Nanogold – were employed. The destiny of internalized plasma membrane domains, carrying negatively or positively charged residues, was followed at the ultrastructural level and revealed distinct endocytic pathways. Time-course experiments and electron microscopy showed internalization of subapical plasma-membrane domains that were mainly recycled to the secretory pathway through the Golgi apparatus and a second mainly degradative pathway involving plasma membrane retrieval at the tip. In vivo time-lapse experiments using FM4-64 combined with quantitative analysis confirmed the existence of distinct internalization regions. Ikarugamycin, an inhibitor of clathrin-dependent endocytosis, allowed us to further dissect the endocytic process: electron microscopy and time-lapse studies suggested that clathrin-dependent endocytosis occurs in the tip and subapical regions, because recycling of positively charged Nanogold to the Golgi bodies and the consignment of negatively charged Nanogold to vacuoles were affected. However, intact positively charged-Nanogold transport to vacuoles supports the idea that an endocytic pathway that does not require clathrin is also present in pollen tubes.
Hesheng Jiang - One of the best experts on this subject based on the ideXlab platform.
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resonance scattering spectral detection of trace hg2 using aptamer modified Nanogold as probe and nanocatalyst
Analytical Chemistry, 2009Co-Authors: Zhiliang Jiang, Aihui Liang, Menglin Chen, Xianjiu Liao, Xingcan Shen, Xingcun He, Hesheng JiangAbstract:Single-strand DNA (ssDNA) was used to modify 10 nm Nanogold to obtain an aptamer-modified Nanogold resonance scattering (RS) probe (AussDNA) for detection of Hg(2+). In the presence of NaCl, Hg(2+) interacts with AussDNA to form very stable double-strand T-Hg(2+)-T mismatches and release Nanogold particles that aggregate to large Nanogold clusters causing the RS intensity at 540 nm to be enhanced linearly. On those grounds, 1.3-1667 nM Hg(2+) can be detected rapidly by the aptamer-modified Nanogold RS assay, with a detection limit of 0.7 nM Hg(2+). If the large Nanogold clusters were removed by membrane filtration, the excess AussDNA in the filtrate solution exhibits a catalytic effect on the new Cu(2)O particle reaction between NH(2)OH and Cu(2+)-EDTA complex at 60 degrees C. The excess AussDNA decreased with the addition of Hg(2+), which led the Cu(2)O particle RS intensity at 602 nm to decrease. The decreased RS intensity (DeltaI(602nm)) had a linear response to Hg(2+) concentration in the range of 0.1-400 nM, with a detection limit of 0.03 nM Hg(2+). This aptamer-modified Nanogold catalytic RS method was applied for the detection of Hg(2+) in water samples, with sensitivity, selectivity, and simplicity.
Marco Caccianiga - One of the best experts on this subject based on the ideXlab platform.
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Inhibition of actin polymerisation by low concentration Latrunculin B affects endocytosis and alters exocytosis in shank and tip of tobacco pollen tubes
Plant Biology, 2012Co-Authors: Alessandro Moscatelli, A. I. Idilli, Simona Rodighiero, Marco CaccianigaAbstract:Pollen tube growth depends on the integrity of the actin cytoskeleton that regulates cytoplasmic streaming and secretion. To clarify whether actin also plays a role in pollen tube endocytosis, Latrunculin B (LatB) was employed in internalisation experiments with tobacco pollen tubes, using the lipophilic dye FM4-64 and charged Nanogold. Time-lapse analysis and dissection of endocytosis allowed us to identify internalisation pathways with different sensitivity to LatB. Co-localisation experiments and ultrastructural observations using positively charged Nanogold revealed that LatB significantly inhibited endocytosis in the pollen tube shank, affecting internalisation of the plasma membrane (PM) recycled for secretion, as well as that conveyed to vacuoles. In contrast, endocytosis of negatively charged Nanogold in the tip, which is also conveyed to vacuoles, was not influenced. Experiments of fluorescence recovery after photobleaching (FRAP) of the apical and subapical PM revealed domains with different rates of fluorescence recovery and showed that these differences depend on the actin cytoskeleton integrity. These results show the presence of distinct degradation pathways by demonstrating that actin-dependent and actin-indepedent endocytosis both operate in pollen tubes, internalising tracts of PM to be recycled and broken down. Intriguingly, although most studies concentrate on exocytosis and distension in the apex, the present paper shows that uncharacterised, actin-dependent secretory activity occurs in the shank of pollen tubes.
