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Jun-jie Zhu - One of the best experts on this subject based on the ideXlab platform.
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aptamer conjugated au nanocage sio2 core shell bifunctional Nanoprobes with high stability and biocompatibility for cellular sers imaging and near infrared photothermal therapy
ACS Sensors, 2019Co-Authors: Shengping Wen, Xuran Miao, Gao-chao Fan, Li-ping Jiang, Chenxin Cai, Jun-jie ZhuAbstract:The combination of surface-enhanced Raman scattering (SERS) imaging technology with near-infrared (NIR) light-triggered photothermal therapy is of utmost importance to develop novel theranostic platforms. Herein, an aptamer-conjugated Au nanocage/SiO2 (AuNC/SiO2/Apt) core-shell Raman nanoprobe has been rationally designed as the bifunctional theranostic platform to fulfill this task. In this theranostic system, the Raman-labeled Au nanocage (AuNC) was encapsulated into a bioinert shell of SiO2, followed by conjugating aptamer AS1411 as the target-recognition moiety. AuNC served as the SERS-active and photothermal substrate due to its large free volume, built-in plasmon effect, and NIR photothermal capacity, while the SiO2 coating endowed the Nanoprobes with good stability and biocompatibility, as well as abundant anchoring sites for surface functionalization. Considering their prominent SERS and photothermal properties, the application potential of the AuNC/SiO2/Apt Nanoprobes was investigated. The proposed Nanoprobes could be applied to targeted detection and SERS imaging of nucleolin-overexpressing cancer cells (MCF-7 cells as the model) from normal cells and also exhibited acceptable photothermal efficacy without systematic toxicity. This theranostic nanoplatform provided a possible opportunity for in situ diagnosis and noninvasive treatment of cancer cells by SERS imaging-guided photothermal therapy.
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Aptamer-Conjugated Au Nanocage/SiO2 Core–Shell Bifunctional Nanoprobes with High Stability and Biocompatibility for Cellular SERS Imaging and Near-Infrared Photothermal Therapy
2019Co-Authors: Shengping Wen, Xuran Miao, Gao-chao Fan, Li-ping Jiang, Chenxin Cai, Jun-jie ZhuAbstract:The combination of surface-enhanced Raman scattering (SERS) imaging technology with near-infrared (NIR) light-triggered photothermal therapy is of utmost importance to develop novel theranostic platforms. Herein, an aptamer-conjugated Au nanocage/SiO2 (AuNC/SiO2/Apt) core–shell Raman nanoprobe has been rationally designed as the bifunctional theranostic platform to fulfill this task. In this theranostic system, the Raman-labeled Au nanocage (AuNC) was encapsulated into a bioinert shell of SiO2, followed by conjugating aptamer AS1411 as the target-recognition moiety. AuNC served as the SERS-active and photothermal substrate due to its large free volume, built-in plasmon effect, and NIR photothermal capacity, while the SiO2 coating endowed the Nanoprobes with good stability and biocompatibility, as well as abundant anchoring sites for surface functionalization. Considering their prominent SERS and photothermal properties, the application potential of the AuNC/SiO2/Apt Nanoprobes was investigated. The proposed Nanoprobes could be applied to targeted detection and SERS imaging of nucleolin-overexpressing cancer cells (MCF-7 cells as the model) from normal cells and also exhibited acceptable photothermal efficacy without systematic toxicity. This theranostic nanoplatform provided a possible opportunity for in situ diagnosis and noninvasive treatment of cancer cells by SERS imaging-guided photothermal therapy
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single gold silver Nanoprobes for real time tracing the entire autophagy process at single cell level
Journal of the American Chemical Society, 2015Co-Authors: Zixuan Chen, Xueqin Chen, Juntao Cao, Jianrong Zhang, Qianhao Min, Jun-jie ZhuAbstract:This article describes a multimodified core–shell gold@silver nanoprobe for real-time monitoring the entire autophagy process at single-cell level. Autophagy is vital for understanding the mechanisms of human pathologies, developing novel drugs, and exploring approaches for autophagy controlling. A major challenge for autophagy study lies in real-time monitoring. One solution might come from real-time detection of in situ superoxide radicals (O2•–), because it is the main regulator of autophagy. In this work, our proposed Nanoprobes were etched by O2•– and gave a notable wavelength change in the plasmon resonance scattering spectra. Both the experimental and simulated results suggested the wavelength change rate correlated well with O2•– level. This response enabled its application in real-time in situ quantification of O2•– during autophagy course. More importantly, with the introduction of “relay probe” operation, two types of O2•–-regulating autophagy processes were successfully traced from the beginni...
Wei Huang - One of the best experts on this subject based on the ideXlab platform.
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Lysosome-Assisted Mitochondrial Targeting Nanoprobe Based on Dye-Modified Upconversion Nanophosphors for Ratiometric Imaging of Mitochondrial Hydrogen Sulfide
2018Co-Authors: Hui Zhao, Chao Yin, Quli Fan, Yufu Tang, Zhen Yang, Qichun Zhang, Wei HuangAbstract:Hydrogen sulfide (H2S) is a versatile modulator in mitochondria and involved in numerous diseases caused by mitochondrial dysfunction. Therefore, many efforts have been made to develop fluorescent probes for mitochondrial H2S detection. However, these cationic small molecule probes are inapplicable for in vivo imaging because of the shallow tissue penetration and poor biostability. Herein, a ratiometric upconversion luminescence nanoprobe with an acid-activated targeting strategy is developed for detecting and bioimaging of mitochondrial H2S. The merocyanine triphenylamine-merocyanine (TPAMC)-modified upconversion nanophosphors, acting as the targeting and response component, are encapsulated into a pH-sensitive husk, composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-(poly(ethylene glycol))-2000] (DSPE-PEG) and poly(l-histidine)-b-PEG, which improved the nanoprobe’s stability during transport in vivo. Under lysosomal pH, the PEG shell is interrupted and the targeting sites are exposed to further attach to mitochondria. Taking advantage of the luminescence resonance energy transfer process between TPAMC and upconversion nanophosphors, the ratiometric detection of mitochondrial H2S can be achieved with high selectivity and sensitivity. Cellular testing reveals the precise targeting to mitochondria via a lysosome delivery process. Importantly, the nanoprobe can be used for monitoring mitochondrial H2S levels in living cells and colon cancer mouse models
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organic nanoprobe cocktails for multilocal and multicolor fluorescence imaging of reactive oxygen species
Advanced Functional Materials, 2017Co-Authors: Chao Yin, Houjuan Zhu, Chen Xie, Lei Zhang, Peng Chen, Quli Fan, Wei HuangAbstract:Hypochlorite (ClO−) as a highly reactive oxygen species not only acts as a powerful “guarder” in innate host defense but also regulates inflammation-related pathological conditions. Despite the availability of fluorescence probes for detection of ClO− in cells, most of them can only detect ClO− in single cellular organelle, limiting the capability to fully elucidate the synergistic effect of different organelles on the generation of ClO−. This study proposes a nanoprobe cocktail approach for multicolor and multiorganelle imaging of ClO− in cells. Two semiconducting oligomers with different π-conjugation length are synthesized, both of which contain phenothiazine to specifically react with ClO− but show different fluorescent color responses. These sensing components are self-assembled into the Nanoprobes with the ability to target cellular lysosome and mitochondria, respectively. The mixture of these Nanoprobes forms a nano-cocktail that allows for simultaneous imaging of elevated level of ClO− in lysosome and mitochondria according to fluorescence color variations under selective excitation of each nanoprobe. Thus, this study provides a general concept to design probe cocktails for multilocal and multicolor imaging.
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degradable semiconducting oligomer amphiphile for ratiometric photoacoustic imaging of hypochlorite
ACS Nano, 2017Co-Authors: Xu Zhen, Wei Huang, Kanyi PuAbstract:Upregulation of highly reactive oxygen species (ROS) such as hypochlorite (ClO–) is associated with many pathological conditions including cardiovascular diseases, neuron degeneration, lung injury, and cancer. However, real-time imaging of ClO– is limited to the probes generally relying on fluorescence with shallow tissue-penetration depth. We here propose a self-assembly approach to develop activatable and degradable photoacoustic (PA) Nanoprobes for in vivo imaging of ClO–. A near-infrared absorbing amphiphilic oligomer is synthesized to undergo degradation in the presence of a specific ROS (ClO–), which integrates a π-conjugated but ClO– oxidizable backbone with hydrophilic PEG side chains. This molecular architecture allows the oligomer to serve as a degradable nanocarrier to encapsulate the ROS-inert dye and self-assemble into structurally stable nanoparticles through both π–π stacking and hydrophobic interactions. The self-assembled nanoprobe exhibits sensitive and specific ratiometric PA signals to...
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simultaneous realization of hg 2 sensing magnetic resonance imaging and upconversion luminescence in vitro and in vivo bioimaging based on hollow mesoporous silica coated ucnps and ruthenium complex
Nanoscale, 2015Co-Authors: Lining Sun, Di Jin, Liang Dong, Liyi Shi, Haige Chen, Wei HuangAbstract:We have constructed a multifunctional nanoprobe with sensing and imaging properties by using hollow mesoporous silica coated upconversion nanoparticles (UCNPs) and Hg2+ responsive ruthenium (Ru) complex. The Ru complex was loaded into the hollow mesoporous silica and the UCNPs acted as an energy donor, transferring luminescence energy to the Ru complex. Furthermore, polyethylenimine (PEI) was assembled on the surface of mesoporous silica to achieve better hydrophilic and bio-compatibility. Upon addition of Hg2+, a blue shift of the absorption peak of the Ru complex is observed and the energy transfer process between the UCNPs and the Ru complex was blocked, resulting in an increase of the green emission intensity of the UCNPs. The un-changed 801 nm emission of the nanoprobe was used as an internal standard reference and the detection limit of Hg2+ was determined to be 0.16 μM for this nanoprobe in aqueous solution. In addition, based on the low cytotoxicity as studied by CCK-8 assay, the nanoprobe was successfully applied for cell imaging and small animal imaging. Furthermore, when doped with Gd3+ ions, the nanoprobe was successfully applied to in vivo magnetic resonance imaging (MRI) of Kunming mice, which demonstrates its potential as a MRI positive-contrast agent. Therefore, the method and results may provide more exciting opportunities to afford Nanoprobes with multimodal bioimaging and multifunctional applications.
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highly selective phosphorescent Nanoprobes for sensing and bioimaging of homocysteine and cysteine
Journal of Materials Chemistry, 2012Co-Authors: Xiangmei Liu, Shujuan Liu, Huiran Yang, Qiang Zhao, Wei HuangAbstract:Most of reported fluorescent probes for mercapto amino acids are organic dyes. They often exhibit poor water-solubility and require the use of biologically toxic organic solvents in sensing and bioimaging. In the present study, a biocompatible phosphorescent nanoprobe by using mesoporous silica nanoparticles as carriers and an iridium(III) complex as signaling units was demonstrated. The nanoprobe exhibits a naked-eye double-signal response for the detection of homocysteine (Hcy) and cysteine (Cys) in pure phosphate buffer saline (PBS), which provides the advantage in effectively avoiding the interference from background signal of biological samples and environmental effects. In addition, the response mechanism, cytotoxicity and bioimaging were studied in detail. These results demonstrated that such a design strategy of phosphorescent Nanoprobes is an effective way to develop excellent phosphorescent cellular probes for live cell applications.
Tuan Vodinh - One of the best experts on this subject based on the ideXlab platform.
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sers based inverse molecular sentinel ims Nanoprobes for multiplexed detection of microrna cancer biomarkers in biological samples
Proceedings of SPIE, 2017Co-Authors: Bridget M. Crawford, Hsin-neng Wang, Andrew M Fales, Michelle L Bowie, Victoria L Seewaldt, Tuan VodinhAbstract:The development of sensitive and selective biosensing techniques is of great interest for clinical diagnostics. Here, we describe the development and application of a surface enhanced Raman scattering (SERS) sensing technology, referred to as "inverse Molecular Sentinel (iMS)" Nanoprobes, for the detection of nucleic acid biomarkers in biological samples. This iMS nanoprobe involves the use of plasmonic-active nanostars as the sensing platform for a homogenous assay for multiplexed detection of nucleic acid biomarkers, including DNA, RNA and microRNA (miRNA). The "OFF-to-ON" signal switch is based on a non-enzymatic strand-displacement process and the conformational change of stem-loop (hairpin) oligonucleotide probes upon target binding. Here, we demonstrate the development of iMS Nanoprobes for the detection of DNA sequences as well as a modified design of the nanoprobe for the detection of short (22-nt) microRNA sequences. The application of iMS Nanoprobes to detect miRNAs in real biological samples was performed with total small RNA extracted from breast cancer cell lines. The multiplex capability of the iMS technique was demonstrated using a mixture of the two differently labeled Nanoprobes to detect miR-21 and miR-34a miRNA biomarkers for breast cancer. The results of this study demonstrate the feasibility of applying the iMS technique for multiplexed detection of nucleic acid biomarkers, including short miRNAs molecules.
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multiplexed detection of microrna biomarkers using sers based inverse molecular sentinel ims Nanoprobes
Journal of Physical Chemistry C, 2016Co-Authors: Hsin-neng Wang, Bridget M. Crawford, Andrew M Fales, Michelle L Bowie, Victoria L Seewaldt, Tuan VodinhAbstract:MicroRNAs (miRNAs) have demonstrated great promise as a novel class of biomarkers for early detection of various cancers, including breast cancer. However, due to technical difficulties in detecting these small molecules, miRNAs have not been adopted into routine clinical practice for early diagnostics. Thus, it is important to develop alternative detection strategies that could offer more advantages over conventional methods. Here, we demonstrate the application of a "turn-on" SERS sensing technology, referred to as "inverse Molecular Sentinel (iMS)" Nanoprobes, as a homogeneous assay for multiplexed detection of miRNAs. This SERS nanoprobe involves the use of plasmonic-active nanostars as the sensing platform. The "OFF-to-ON" signal switch is based on a nonenzymatic strand-displacement process and the conformational change of stem-loop (hairpin) oligonucleotide probes upon target binding. This technique was previously used to detect a synthetic DNA sequence of interest. In this study, we modified the design of the nanoprobe to be used for the detection of short (22-nt) miRNA sequences. The demonstration of using iMS Nanoprobes to detect miRNAs in real biological samples was performed with total small RNA extracted from breast cancer cell lines. The multiplex capability of the iMS technique was demonstrated using a mixture of the two differently labeled Nanoprobes to detect miR-21 and miR-34a miRNA biomarkers for breast cancer. The results of this study demonstrate the feasibility of applying the iMS technique for multiplexed detection of short miRNAs molecules.
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multiplexed detection of microrna biomarkers using sers based inverse molecular sentinel ims Nanoprobes
Journal of Physical Chemistry C, 2016Co-Authors: Hsin-neng Wang, Bridget M. Crawford, Andrew M Fales, Michelle L Bowie, Victoria L Seewaldt, Tuan VodinhAbstract:MicroRNAs (miRNAs) have demonstrated great promise as a novel class of biomarkers for early detection of various cancers, including breast cancer. However, due to technical difficulties in detecting these small molecules, miRNAs have not been adopted into routine clinical practice for early diagnostics. Thus, it is important to develop alternative detection strategies that could offer more advantages over conventional methods. Here, we demonstrate the application of a “turn-on” SERS sensing technology, referred to as “inverse Molecular Sentinel (iMS)” Nanoprobes, as a homogeneous assay for multiplexed detection of miRNAs. This SERS nanoprobe involves the use of plasmonic-active nanostars as the sensing platform. The “OFF-to-ON” signal switch is based on a nonenzymatic strand-displacement process and the conformational change of stem-loop (hairpin) oligonucleotide probes upon target binding. This technique was previously used to detect a synthetic DNA sequence of interest. In this study, we modified the de...
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detection of human immunodeficiency virus type 1 dna sequence using plasmonics Nanoprobes
Analytical Chemistry, 2005Co-Authors: Musundi B Wabuyele, Tuan VodinhAbstract:This paper describes the use of plasmonics-based Nanoprobes that act as molecular sentinels for DNA diagnostics. The plasmonics nanoprobe comprises a metal nanoparticle and a stem-loop DNA molecule tagged with a Raman label. The nanoprobe utilizes the specificity and selectivity of the DNA hairpin probe sequence to detect a specific target DNA sequence of interest. In the absence of target DNA, the stem-loop configuration maintains the Raman label in proximity to the metal nanoparticle, inducing an intense surface-enhanced Raman scattering (SERS) effect that produces a strong Raman signal upon laser excitation. Upon hybridization of a complementary target DNA sequence to the nanoprobe, the stem-loop configuration is disrupted, causing the Raman label to physically separate from the metal nanoparticle, thus quenching the SERS signal. The usefulness and potential application of the plasmonics nanoprobe for diagnosis is demonstrated using the gag gene sequence of the human immunodeficiency virus type 1 (HIV-1). We successfully demonstrated the specificity and selectivity of the plasmonics Nanoprobes to detect PCR amplicons of the HIV gene. The potential for combining the spectral selectivity and high sensitivity of the SERS process with inherent molecular specificity of DNA hairpins to diagnose molecular target sequences in homogeneous solutions is discussed.
Chao Yin - One of the best experts on this subject based on the ideXlab platform.
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Lysosome-Assisted Mitochondrial Targeting Nanoprobe Based on Dye-Modified Upconversion Nanophosphors for Ratiometric Imaging of Mitochondrial Hydrogen Sulfide
2018Co-Authors: Hui Zhao, Chao Yin, Quli Fan, Yufu Tang, Zhen Yang, Qichun Zhang, Wei HuangAbstract:Hydrogen sulfide (H2S) is a versatile modulator in mitochondria and involved in numerous diseases caused by mitochondrial dysfunction. Therefore, many efforts have been made to develop fluorescent probes for mitochondrial H2S detection. However, these cationic small molecule probes are inapplicable for in vivo imaging because of the shallow tissue penetration and poor biostability. Herein, a ratiometric upconversion luminescence nanoprobe with an acid-activated targeting strategy is developed for detecting and bioimaging of mitochondrial H2S. The merocyanine triphenylamine-merocyanine (TPAMC)-modified upconversion nanophosphors, acting as the targeting and response component, are encapsulated into a pH-sensitive husk, composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-(poly(ethylene glycol))-2000] (DSPE-PEG) and poly(l-histidine)-b-PEG, which improved the nanoprobe’s stability during transport in vivo. Under lysosomal pH, the PEG shell is interrupted and the targeting sites are exposed to further attach to mitochondria. Taking advantage of the luminescence resonance energy transfer process between TPAMC and upconversion nanophosphors, the ratiometric detection of mitochondrial H2S can be achieved with high selectivity and sensitivity. Cellular testing reveals the precise targeting to mitochondria via a lysosome delivery process. Importantly, the nanoprobe can be used for monitoring mitochondrial H2S levels in living cells and colon cancer mouse models
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organic nanoprobe cocktails for multilocal and multicolor fluorescence imaging of reactive oxygen species
Advanced Functional Materials, 2017Co-Authors: Chao Yin, Houjuan Zhu, Chen Xie, Lei Zhang, Peng Chen, Quli Fan, Wei HuangAbstract:Hypochlorite (ClO−) as a highly reactive oxygen species not only acts as a powerful “guarder” in innate host defense but also regulates inflammation-related pathological conditions. Despite the availability of fluorescence probes for detection of ClO− in cells, most of them can only detect ClO− in single cellular organelle, limiting the capability to fully elucidate the synergistic effect of different organelles on the generation of ClO−. This study proposes a nanoprobe cocktail approach for multicolor and multiorganelle imaging of ClO− in cells. Two semiconducting oligomers with different π-conjugation length are synthesized, both of which contain phenothiazine to specifically react with ClO− but show different fluorescent color responses. These sensing components are self-assembled into the Nanoprobes with the ability to target cellular lysosome and mitochondria, respectively. The mixture of these Nanoprobes forms a nano-cocktail that allows for simultaneous imaging of elevated level of ClO− in lysosome and mitochondria according to fluorescence color variations under selective excitation of each nanoprobe. Thus, this study provides a general concept to design probe cocktails for multilocal and multicolor imaging.
Min-ho Lee - One of the best experts on this subject based on the ideXlab platform.
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robust bioengineered apoferritin Nanoprobes for ultrasensitive detection of infectious pancreatic necrosis virus
Analytical Chemistry, 2019Co-Authors: Sachin Ganpat Chavan, Ajay Kumar Yagati, Mohsen Mohammadniaei, Junhong Min, Min-ho LeeAbstract:Infectious pancreatic necrosis virus (IPNV) has been identified as a viral pathogen for many fish diseases that have become a huge hurdle for the growing fishing industry. Thus, in this work, we report a label-free impedance biosensor to quantify IPNV in real fish samples at point-of-care (POC) level. High specificity IPNV sensor with a detection limit of 2.69 TCID50/mL was achieved by conjugating IPNV antibodies to portable Au disk electrode chips using human heavy chain apoferritin (H-AFN) Nanoprobes as a binding agent. H-AFN probes were bioengineered through PCR by incorporating pET-28b(+) resulting in 24 subunits of 6 × his-tag and protein-G units on its outer surface to increase the sensitivity of the IPNV detection. The biosensor surface modifications were characterized by differential pulse voltammetry (DPV) and EIS methods for each modification step. The proposed nanoprobe based sensor showed three-fold enhancement in charge transfer resistance toward IPNV detection in comparison with the traditional linker approach when measured in a group of similar virus molecules. The portable sensor exhibited a linear range of 100-10000 TCID50/mL and sensitivity of 5.40 × 10-4 TCID50/mL in real-fish samples. The performance of the proposed IPNV sensor was fully validated using an enzyme-linked immunosorbent assay (ELISA) technique with a sensitivity of 3.02 × 10-4 TCID50/mL. Results from H-AFN nanoprobe based IPNV sensor indicated high selectivity, sensitivity, and stability could be a promising platform for the detection of similar fish viruses and other biological molecules of interest.
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Robust Bioengineered Apoferritin Nanoprobes for Ultrasensitive Detection of Infectious Pancreatic Necrosis Virus
2019Co-Authors: Sachin Ganpat Chavan, Ajay Kumar Yagati, Mohsen Mohammadniaei, Junhong Min, Min-ho LeeAbstract:Infectious pancreatic necrosis virus (IPNV) has been identified as a viral pathogen for many fish diseases that have become a huge hurdle for the growing fishing industry. Thus, in this work, we report a label-free impedance biosensor to quantify IPNV in real fish samples at point-of-care (POC) level. High specificity IPNV sensor with a detection limit of 2.69 TCID50/mL was achieved by conjugating IPNV antibodies to portable Au disk electrode chips using human heavy chain apoferritin (H-AFN) Nanoprobes as a binding agent. H-AFN probes were bioengineered through PCR by incorporating pET-28b(+) resulting in 24 subunits of 6 × his-tag and protein-G units on its outer surface to increase the sensitivity of the IPNV detection. The biosensor surface modifications were characterized by differential pulse voltammetry (DPV) and EIS methods for each modification step. The proposed nanoprobe based sensor showed three-fold enhancement in charge transfer resistance toward IPNV detection in comparison with the traditional linker approach when measured in a group of similar virus molecules. The portable sensor exhibited a linear range of 100–10000 TCID50/mL and sensitivity of 5.40 × 10–4 TCID50/mL in real-fish samples. The performance of the proposed IPNV sensor was fully validated using an enzyme-linked immunosorbent assay (ELISA) technique with a sensitivity of 3.02 × 10–4 TCID50/mL. Results from H-AFN nanoprobe based IPNV sensor indicated high selectivity, sensitivity, and stability could be a promising platform for the detection of similar fish viruses and other biological molecules of interest
