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Anna Menini - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Development of the Olfactory Epithelium and Nasal Glands in TMEM16A-/- and TMEM16A+/ + Mice
2016Co-Authors: Devendra Kumar Maurya, Tiago Henriques, Monica Marini, Nicoletta Pedemonte, Jason R. Rock, Brian D. Harfe, J. V. Galietta, Anna MeniniAbstract:TMEM16A/ANO1 is a calcium-activated chloride channel expressed in several types of epi-thelia and involved in various physiological processes, including proliferation and develop-ment. During mouse embryonic development, the expression of TMEM16A in the olfactory epithelium is dynamic. TMEM16A is expressed at the apical surface of the entire olfactory epithelium at embryonic day E12.5 while from E16.5 its expression is restricted to a region near the transition zone with the respiratory epithelium. To investigate whether TMEM16A plays a role in the development of the mouse olfactory epithelium, we obtained the first immunohistochemistry study comparing the morphological properties of the olfactory epi-thelium and Nasal Glands in TMEM16A-/- and TMEM16A+/+ littermate mice. A comparison between the expression of the olfactory marker protein and adenylyl cyclase III shows that genetic ablation of TMEM16A did not seem to affect the maturation of olfactory sensory neurons and their ciliary layer. As TMEM16A is expressed at the apical part of supporting cells and in their microvilli, we used ezrin and cytokeratin 8 as markers of microvilli and cell body of supporting cells, respectively, and found that morphology and development of sup
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development of the olfactory epithelium and Nasal Glands in tmem16a and tmem16a mice
2015Co-Authors: Devendra Kumar Maurya, Tiago Henriques, Monica Marini, Nicoletta Pedemonte, Luis J. V. Galietta, Jason R. Rock, Brian D. Harfe, Anna MeniniAbstract:TMEM16A/ANO1 is a calcium-activated chloride channel expressed in several types of epithelia and involved in various physiological processes, including proliferation and development. During mouse embryonic development, the expression of TMEM16A in the olfactory epithelium is dynamic. TMEM16A is expressed at the apical surface of the entire olfactory epithelium at embryonic day E12.5 while from E16.5 its expression is restricted to a region near the transition zone with the respiratory epithelium. To investigate whether TMEM16A plays a role in the development of the mouse olfactory epithelium, we obtained the first immunohistochemistry study comparing the morphological properties of the olfactory epithelium and Nasal Glands in TMEM16A-/- and TMEM16A+/+ littermate mice. A comparison between the expression of the olfactory marker protein and adenylyl cyclase III shows that genetic ablation of TMEM16A did not seem to affect the maturation of olfactory sensory neurons and their ciliary layer. As TMEM16A is expressed at the apical part of supporting cells and in their microvilli, we used ezrin and cytokeratin 8 as markers of microvilli and cell body of supporting cells, respectively, and found that morphology and development of supporting cells were similar in TMEM16A-/- and TMEM16A+/+ littermate mice. The average number of supporting cells, olfactory sensory neurons, horizontal and globose basal cells were not significantly different in the two types of mice. Moreover, we also observed that the morphology of Bowman’s Glands, Nasal septal Glands and lateral Nasal Glands did not change in the absence of TMEM16A. Our results indicate that the development of mouse olfactory epithelium and Nasal Glands does not seem to be affected by the genetic ablation of TMEM16A.
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Schematic representations and confocal images of nose coronal sections.
2015Co-Authors: Devendra Kumar Maurya, Tiago Henriques, Monica Marini, Nicoletta Pedemonte, Luis J. V. Galietta, Jason R. Rock, Brian D. Harfe, Anna MeniniAbstract:A: Schematic diagram of a nose coronal section showing the olfactory epithelium, respiratory epithelium, Bowman’s Glands, Nasal septal Glands, and lateral Nasal Glands. B: The pseudostratified olfactory epithelium is mainly composed of supporting cells, olfactory sensory neurons and basal cells. Supporting cells have columnar cell bodies, microvilli at the apical side, and reside in the most apical region of the epithelium. Mature and immature olfactory sensory neurons are bipolar neurons with a single dendritic process projecting toward the apical surface of the epithelium. In mature olfactory sensory neurons, several cilia protrude from the dendritic knob. Two types of basal cells reside near the basal lamina: horizontal basal cells (HBCs) that are attached to the basal lamina, and globose basal cells (GBCs) that are located above the HBC layer. C-D: confocal images of coronal sections of the olfactory epithelium at E12.5 and P4. C: At E12.5, TMEM16A was expressed at the surface of the entire olfactory epithelium. D: At P4, TMEM16A immunoreactivity was present only in the olfactory regions toward the respiratory epithelium. Arrows in (D) indicate the transition between olfactory and respiratory epithelium. The apical surface of the olfactory epithelium was well stained by adenylyl cyclase III (ACIII). Higher magnification images taken from the boxed areas are shown in D1 and D2. Cell nuclei were stained by DAPI. Scale bars: C = 50 μm; D = 100 μm; D1-D2 = 20 μm.
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Expression of TMEM16A in various Nasal Glands of TMEM16A-/- and TMEM16A+/+ littermate mice.
2015Co-Authors: Devendra Kumar Maurya, Tiago Henriques, Monica Marini, Nicoletta Pedemonte, Luis J. V. Galietta, Jason R. Rock, Brian D. Harfe, Anna MeniniAbstract:Expression of TMEM16A and aquaporin 5 in the Bowman’s gland (BG), Nasal septal gland (NSG) and lateral Nasal gland (LNG) of TMEM16A-/- and TMEM16A+/+ littermate mice. A, D: aquaporin 5 immunopositive signals were seen in Bowman’s Glands in P4 mice. Aquaporin 5 expression in Glands and ducts is clearly visible. TMEM16A immunoreactivity (goat anti-TMEM16A) was not present in Bowman’s Glands of TMEM16A-/- nor of TMEM16A+/+ littermate mice (A, D). However, aquaporin 5 and TMEM16A were co-expressed in Nasal septal Glands (B) and lateral Nasal Glands (C) of TMEM16A+/+ mice. No immunoreactivity to TMEM16A was detectable in TMEM16A-/- mice (E, F). Glands marked by aquaporin 5 were similar in both types of mice. Images are averages of z-stacks of thickness of ~2.0 μm for A, D, or ~1 μm for B, C, E, F. Cell nuclei were stained by DAPI. Scale bars = 10 μm.
Masaya Tohyama - One of the best experts on this subject based on the ideXlab platform.
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expression of major urinary protein genes in the Nasal Glands associated with general olfaction
1999Co-Authors: Makio Utsumi, Koji Ohno, Takeshi Kubo, Yoshiaki Kawasaki, Manabu Tamura, Masaya TohyamaAbstract:Gene expression of major urinary protein (MUP) mRNAs was examined in the mouse Nasal tissue. By polymerase chain reaction, we identified two cDNA segments encoding MUP 4 and MUP 5 genes in the nose. The expression level of both MUP 4 and 5 mRNAs in the Nasal tissue was very high and exceeded that of the liver. Liver MUPs are excreted into the urine and are known to play an important role in pheromonal communication. We showed that nose and liver MUPs were composed of different subtypes of MUPs and that nose MUP mRNAs was detected in prepubescent periods when liver MUP mRNAs had not yet been transcripted. In situ hybridization revealed that nose MUP mRNAs are localized in the lateral wall and Nasal septum and their expression pattern is identical to that of rat odorant-binding protein (OBP)-I. We also identified cDNA of mouse OBP-II gene from the Nasal tissue and showed that the expression pattern of MUP gene was identical to that of OBP-II gene in the nose. These histological data indicate that nose MUPs are favorable for catching odorant molecules rather than pheromones, and may share their function with OBPs.
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Differential expression of odorant-binding protein genes in rat Nasal Glands: Implications for odorant-binding proteinII as a possible pheromone transporter
1996Co-Authors: Koji Ohno, Y. Kawasaki, Takeshi Kubo, Masaya TohyamaAbstract:Abstract We examined the distribution and ontogeny of two odorant-binding proteins in the rat at various stages of development from newborn to adult using northern blot and in situ hybridization methods. Our results demonstrated spatial segregation between odorant-binding protein and odorant-binding proteinII in Nasal glandular tissues. Odorant-binding protein messenger RNA was expressed in the glandular system opening into the Nasal vestibule, whereas odorant-binding proteinII messenger RNA was seen in the posterior Glands of the Nasal septum and in the vomeroNasal Glands. In addition, odorant-binding protein and odorant-binding proteinII messenger RNA levels increased during early postnatal stages with time courses that paralleled the anatomical development of the main olfactory system and the vomeroNasal system, respectively. Our results suggest that odorant-binding protein, functions as a pheromone transporter in the vomeroNasal system.
Devendra Kumar Maurya - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Development of the Olfactory Epithelium and Nasal Glands in TMEM16A-/- and TMEM16A+/ + Mice
2016Co-Authors: Devendra Kumar Maurya, Tiago Henriques, Monica Marini, Nicoletta Pedemonte, Jason R. Rock, Brian D. Harfe, J. V. Galietta, Anna MeniniAbstract:TMEM16A/ANO1 is a calcium-activated chloride channel expressed in several types of epi-thelia and involved in various physiological processes, including proliferation and develop-ment. During mouse embryonic development, the expression of TMEM16A in the olfactory epithelium is dynamic. TMEM16A is expressed at the apical surface of the entire olfactory epithelium at embryonic day E12.5 while from E16.5 its expression is restricted to a region near the transition zone with the respiratory epithelium. To investigate whether TMEM16A plays a role in the development of the mouse olfactory epithelium, we obtained the first immunohistochemistry study comparing the morphological properties of the olfactory epi-thelium and Nasal Glands in TMEM16A-/- and TMEM16A+/+ littermate mice. A comparison between the expression of the olfactory marker protein and adenylyl cyclase III shows that genetic ablation of TMEM16A did not seem to affect the maturation of olfactory sensory neurons and their ciliary layer. As TMEM16A is expressed at the apical part of supporting cells and in their microvilli, we used ezrin and cytokeratin 8 as markers of microvilli and cell body of supporting cells, respectively, and found that morphology and development of sup
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development of the olfactory epithelium and Nasal Glands in tmem16a and tmem16a mice
2015Co-Authors: Devendra Kumar Maurya, Tiago Henriques, Monica Marini, Nicoletta Pedemonte, Luis J. V. Galietta, Jason R. Rock, Brian D. Harfe, Anna MeniniAbstract:TMEM16A/ANO1 is a calcium-activated chloride channel expressed in several types of epithelia and involved in various physiological processes, including proliferation and development. During mouse embryonic development, the expression of TMEM16A in the olfactory epithelium is dynamic. TMEM16A is expressed at the apical surface of the entire olfactory epithelium at embryonic day E12.5 while from E16.5 its expression is restricted to a region near the transition zone with the respiratory epithelium. To investigate whether TMEM16A plays a role in the development of the mouse olfactory epithelium, we obtained the first immunohistochemistry study comparing the morphological properties of the olfactory epithelium and Nasal Glands in TMEM16A-/- and TMEM16A+/+ littermate mice. A comparison between the expression of the olfactory marker protein and adenylyl cyclase III shows that genetic ablation of TMEM16A did not seem to affect the maturation of olfactory sensory neurons and their ciliary layer. As TMEM16A is expressed at the apical part of supporting cells and in their microvilli, we used ezrin and cytokeratin 8 as markers of microvilli and cell body of supporting cells, respectively, and found that morphology and development of supporting cells were similar in TMEM16A-/- and TMEM16A+/+ littermate mice. The average number of supporting cells, olfactory sensory neurons, horizontal and globose basal cells were not significantly different in the two types of mice. Moreover, we also observed that the morphology of Bowman’s Glands, Nasal septal Glands and lateral Nasal Glands did not change in the absence of TMEM16A. Our results indicate that the development of mouse olfactory epithelium and Nasal Glands does not seem to be affected by the genetic ablation of TMEM16A.
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Schematic representations and confocal images of nose coronal sections.
2015Co-Authors: Devendra Kumar Maurya, Tiago Henriques, Monica Marini, Nicoletta Pedemonte, Luis J. V. Galietta, Jason R. Rock, Brian D. Harfe, Anna MeniniAbstract:A: Schematic diagram of a nose coronal section showing the olfactory epithelium, respiratory epithelium, Bowman’s Glands, Nasal septal Glands, and lateral Nasal Glands. B: The pseudostratified olfactory epithelium is mainly composed of supporting cells, olfactory sensory neurons and basal cells. Supporting cells have columnar cell bodies, microvilli at the apical side, and reside in the most apical region of the epithelium. Mature and immature olfactory sensory neurons are bipolar neurons with a single dendritic process projecting toward the apical surface of the epithelium. In mature olfactory sensory neurons, several cilia protrude from the dendritic knob. Two types of basal cells reside near the basal lamina: horizontal basal cells (HBCs) that are attached to the basal lamina, and globose basal cells (GBCs) that are located above the HBC layer. C-D: confocal images of coronal sections of the olfactory epithelium at E12.5 and P4. C: At E12.5, TMEM16A was expressed at the surface of the entire olfactory epithelium. D: At P4, TMEM16A immunoreactivity was present only in the olfactory regions toward the respiratory epithelium. Arrows in (D) indicate the transition between olfactory and respiratory epithelium. The apical surface of the olfactory epithelium was well stained by adenylyl cyclase III (ACIII). Higher magnification images taken from the boxed areas are shown in D1 and D2. Cell nuclei were stained by DAPI. Scale bars: C = 50 μm; D = 100 μm; D1-D2 = 20 μm.
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Expression of TMEM16A in various Nasal Glands of TMEM16A-/- and TMEM16A+/+ littermate mice.
2015Co-Authors: Devendra Kumar Maurya, Tiago Henriques, Monica Marini, Nicoletta Pedemonte, Luis J. V. Galietta, Jason R. Rock, Brian D. Harfe, Anna MeniniAbstract:Expression of TMEM16A and aquaporin 5 in the Bowman’s gland (BG), Nasal septal gland (NSG) and lateral Nasal gland (LNG) of TMEM16A-/- and TMEM16A+/+ littermate mice. A, D: aquaporin 5 immunopositive signals were seen in Bowman’s Glands in P4 mice. Aquaporin 5 expression in Glands and ducts is clearly visible. TMEM16A immunoreactivity (goat anti-TMEM16A) was not present in Bowman’s Glands of TMEM16A-/- nor of TMEM16A+/+ littermate mice (A, D). However, aquaporin 5 and TMEM16A were co-expressed in Nasal septal Glands (B) and lateral Nasal Glands (C) of TMEM16A+/+ mice. No immunoreactivity to TMEM16A was detectable in TMEM16A-/- mice (E, F). Glands marked by aquaporin 5 were similar in both types of mice. Images are averages of z-stacks of thickness of ~2.0 μm for A, D, or ~1 μm for B, C, E, F. Cell nuclei were stained by DAPI. Scale bars = 10 μm.
Tomomi Nemoto - One of the best experts on this subject based on the ideXlab platform.
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two photon microscopic analysis of acetylcholine induced mucus secretion in guinea pig Nasal Glands
2005Co-Authors: Akihiro Oshima, Tatsuya Kojima, Kenji Dejima, Yasuo Hisa, Haruo Kasai, Tomomi NemotoAbstract:Abstract The spatiotemporal changes in intracellular free Ca2+ concentration ([Ca2+]i) as well as fluid secretion and exocytosis induced by acetylcholine (ACh) in intact acini of guinea pig Nasal Glands were investigated by two-photon excitation imaging. Cross-sectional images of acini loaded with the fluorescent Ca2+ indicator fura-2 revealed that the ACh-evoked increase in [Ca2+]i was immediate and spread from the apical region (the secretory pole) of acinar cells to the basal region. Immersion of acini in a solution containing a fluorescent polar tracer, sulforhodamine B (SRB), revealed that fluid secretion, detected as a rapid disappearance of SRB fluorescence from the extracellular space, occurred exclusively in the luminal region and was accompanied by a reduction in acinar cell volume. Individual exocytic events were also visualized with SRB as the formation of Ω-shaped profiles at the apical membrane. In contrast to the rapidity of fluid secretion, exocytosis of secretory granules occurred with a delay of ∼70 s relative to the increase in [Ca2+]i. Exocytic events also occurred deep within the cytoplasm in a sequential manner with the latency of secondary exocytosis being greatly reduced compared with that of primary exocytosis. The delay in sequential compound exocytosis relative to fluid secretion may be important for release of the viscous contents of secretory granules into the Nasal cavity.
Thomas V. Getchell - One of the best experts on this subject based on the ideXlab platform.
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Differential expression of vomeromodulin and odorant-binding protein, putative pheromone and odorant transporters, in the developing rat Nasal chemosensory mucosae
1995Co-Authors: N. S. Rama Krishna, Marilyn L. Getchell, F. L. Margolis, Thomas V. GetchellAbstract:Expression of the putative pheromone and odorant transporter, vomeromodulin, was characterized in developing rat Nasal mucosae using in situ hybridization and immunocytochemistry. Initial expression of vomeromodulin mRNA and protein was detected at embryonic day (E)16 in the maxillary sinus component of the lateral Nasal Glands. The abundance of mRNA and protein in the lateral Nasal Glands increased with age and reached a peak at postnatal day (P)27. Also at P27, vomeromodulin mRNA and protein expression was initiated in vomeroNasal Glands and posterior Glands of the Nasal septum. Comparison of the developmental expression of odorant-binding protein, another carrier protein synthesized in the lateral Nasal Glands, with that of vomeromodulin demonstrated major differences. In contrast to vomeromodulin, odorant-binding protein was not detected until postnatal day 2 in the ventral component of the lateral Nasal Glands and anterior Glands of the Nasal septum. These results suggest that the expression of vomeromodulin and odorant-binding protein is developmentally and differentially regulated and confirms the suggestion that vomeromodulin may function in olfactory and vomeroNasal perireceptor processes as a transporter for pheromones and odorants. In addition, the embryonic expression of vomeromodulin suggests its involvement in olfactory perireceptor processes in utero. © 1995 Wiley-Liss, Inc.
