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Leam Sreng - One of the best experts on this subject based on the ideXlab platform.
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sensory asymmetries in the olfactory system underlie sexual pheromone communication in the cockroach Nauphoeta cinerea
Neuroscience Letters, 2003Co-Authors: Leam SrengAbstract:In most insect species, males process female sex pheromone information in specific macroglomeruli in the olfactory lobes of their brains. The purpose of this report is to present a novel system of pheromone perception. In Nauphoeta cinerea cockroaches, males produce sex pheromones that induce mating behavior in females but neither sex has macroglomeruli. In this study, we show that female N. cinerea show asymmetry in the number of glomeruli whereas males do not. Females have more glomeruli on the right side and amputation of the right antenna reduced female ability to find a male and mate whereas amputation of the left antenna did not. We show that a key factor in female mating behavior is perception of the male sex pheromone seducin by the right antenna and the asymmetrically organized deutocerebral glomeruli associated with it. In the symmetrical olfactory system of males the detection of seducin may be involved in establishing dominance hierarchies.
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cuticular hydrocarbon profiles of dominant versus subordinate male Nauphoeta cinerea cockroaches
Journal of Chemical Ecology, 2002Co-Authors: Estelle Roux, Leam Sreng, Eric Provost, Maurice Roux, Jeanluc ClementAbstract:Interactions between male Nauphoeta cinerea cockroaches are characterized by an elaborate ritual that leads to a stable dominant–subordinate hierarchy between two individuals. Chemical signals involving volatile sex pheromones and cuticular hydrocarbons play an important role in establishing and maintaining dominance status. The present study was performed to identify cuticular hydrocarbons in two- and three-times dominant or subordinate individuals obtained by forcing dyadic encounters. Two methods, i.e., solid-phase microextraction (SPME) and organic solvent extraction, were used to isolate cuticular hydrocarbons. A total of 23 peaks of cuticular hydrocarbons were identified. Analysis showed quantitative differences in hydrocarbon profiles of three-times dominant and subordinate individuals according to extraction method and dominance status. Dominant individuals were characterized by higher proportions of the monomethylalkanes 11- and 13-MeC36, 13- and 15-MeC38, and 11-, 13-, and 15-MeC35, while subordinate individuals had higher proportions of the following monomethylalkanes: 7-, 9-, and 11-MeC31, 5-MeC31, 3- and 8-MeC32, and 9-, 10- , 11-, and 12-MeC32. Discussion focuses on the reliability of hydrocarbons as indicators of dominance status and on their correlation with physiological processes.
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seducin male sex pheromone of the cockroach Nauphoeta cinerea isolation identification and bioassay
Journal of Chemical Ecology, 1990Co-Authors: Leam SrengAbstract:The male sex pheromone of the cockroachNauphoeta cinerea was isolated from adult sternal glands and identified by gas chromatography-mass spectrometry. This pheromone, which attracts females from a distance, is a multicomponent blend. It is composed principally of three compounds: 3-hydroxy-2-butanone (acetoin), 2-methylthiazolidine, and 4-ethyl-2-meth-oxyphenol. A mixture of synthetic compounds in a ratio of 4∶4∶1 (2700 ng) elicits maximal response and short latency of response by receptive females, similar to that induced by male sternal gland extracts. Tested separately, each compound induces a positive response, but the moving times spent by females are generally longer than with the mixture or extracts. These three volatile compounds also are found in small amounts (about 1/40 of sternal glands) in tergal gland secretions licked by females. Acetoin also is found in the sternal and tergal glands of two closely related speciesLeucophaea maderae andHenschoutedenia flexivitta. The biological relationship of each compound and pheromone blend to behavioral response is discussed.
Craig R White - One of the best experts on this subject based on the ideXlab platform.
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Oxygen-induced plasticity in tracheal morphology and discontinuous gas exchange cycles in cockroaches Nauphoeta cinerea
Journal of Comparative Physiology B, 2014Co-Authors: Hamish Bartrim, Philip G D Matthews, Sussan Lemon, Craig R WhiteAbstract:The function and mechanism underlying discontinuous gas exchange in terrestrial arthropods continues to be debated. Three adaptive hypotheses have been proposed to explain the evolutionary origin or maintenance of discontinuous gas exchange cycles (DGCs), which may have evolved to reduce respiratory water loss, facilitate gas exchange in high CO_2 and low O_2 micro-environments, or to ameliorate potential damage as a result of oversupply of O_2. None of these hypotheses have unequivocal support, and several non-adaptive hypotheses have also been proposed. In the present study, we reared cockroaches Nauphoeta cinerea in selected levels of O_2 throughout development, and examined how this affected growth rate, tracheal morphology and patterns of gas exchange. O_2 level in the rearing environment caused significant changes in tracheal morphology and the exhibition of DGCs, but the direction of these effects was inconsistent with all three adaptive hypotheses: water loss was not associated with DGC length, cockroaches grew fastest in hyperoxia, and DGCs exhibited by cockroaches reared in normoxia were shorter than those exhibited by cockroaches reared in hypoxia or hyperoxia.
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discontinuous gas exchange exhibition is a heritable trait in speckled cockroaches Nauphoeta cinerea
Journal of Evolutionary Biology, 2013Co-Authors: Natalie G Schimpf, Philip G D Matthews, Craig R WhiteAbstract:The function and mechanism underlying discontinuous gas exchange in terrestrial arthropods continues to be debated. Three adaptive hypotheses have been proposed to explain the evolutionary origin or maintenance of discontinuous gas exchange cycles (DGCs), which may have evolved to reduce respiratory water loss, facilitate gas exchange in high CO2 and low O2 micro-environments, or to ameliorate potential damage as a result of oversupply of O2. None of these hypotheses have unequivocal support, and several non-adaptive hypotheses have also been proposed. In the present study, we reared cockroaches Nauphoeta cinerea in selected levels of O2 throughout development, and examined how this affected growth rate, tracheal morphology and patterns of gas exchange. O2 level in the rearing environment caused significant changes in tracheal morphology and the exhibition of DGCs, but the direction of these effects was inconsistent with all three adaptive hypotheses: water loss was not associated with DGC length, cockroaches grew fastest in hyperoxia, and DGCs exhibited by cockroaches reared in normoxia were shorter than those exhibited by cockroaches reared in hypoxia or hyperoxia.
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standard metabolic rate is associated with gestation duration but not clutch size in speckled cockroaches Nauphoeta cinerea
Biology Open, 2012Co-Authors: Natalie G Schimpf, Philip G D Matthews, Craig R WhiteAbstract:Metabolic rate varies significantly between individuals, and these differences persist even when the wide range of biotic and abiotic factors that influence metabolism are accounted for. It is important to understand the life history implications of variation in metabolic rate, but they remain poorly characterised despite a growing body of work examining relationships between metabolism and a range of traits. In the present study we used laboratory-bred families (one sire to three dams) of Nauphoeta cinerea (Olivier) (speckled cockroaches) to examine the relationship between standard metabolic rate (SMR) and reproductive performance (number of offspring and gestation duration). We show that SMR is negatively associated with female gestation duration. Age at mating is negatively associated with gestation duration for females, and mass is negatively associated with the average gestation duration of the females a male was mated with. In addition to the results in the current literature, the results from the present study suggest that the association between metabolism and life history is more complex than simple relationships between metabolism and various fitness traits. Future work should consider longitudinal, ontogenetic as well as selective and quantitative genetic breeding approaches to fully examine the associations between metabolism and fitness.
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regulation of gas exchange and haemolymph ph in the cockroach Nauphoeta cinerea
The Journal of Experimental Biology, 2011Co-Authors: Philip G D Matthews, Craig R WhiteAbstract:Ventilatory control of internal CO2 plays an important role in regulating extracellular acid–base balance in terrestrial animals. While this phenomenon is well understood among vertebrates, the role that respiration plays in the acid–base balance of insects is in need of much further study. To measure changes in insect haemolymph pH, we implanted micro pH optodes into the haemocoel of cockroaches ( Nauphoeta cinerea ). They were then exposed to normoxic, hypoxic, hyperoxic and hypercapnic atmospheres while their haemolymph pH, ![Graphic][1] and abdominal ventilation frequency were measured simultaneously. Intratracheal O2 levels were also measured in separate experiments. It was found that cockroaches breathing continuously control their ventilation to defend a haemolymph pH of 7.3, except under conditions where hypoxia ( 1% CO2). In contrast, intratracheal O2 levels fluctuated widely, but on average remained above 15% in normoxic (21% O2) atmospheres. Decapitation caused the cockroaches to display discontinuous gas exchange cycles (DGCs). The alternating periods of ventilation and apnoea during DGCs caused haemolymph pH to fluctuate by 0.11 units. Exposure to hypoxia caused haemolymph pH to increase and initiated brief bouts of spiracular opening prior to the active ventilation phase. The spontaneous occurrence of DGCs in decapitated cockroaches indicates that central pattern generators in the thoracic and abdominal ganglia generate the periodic gas exchange pattern in the absence of control from the cephalic ganglion. This pattern continues to maintain gas exchange, but with less precision. [1]: /embed/inline-graphic-1.gif
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Regulation of gas exchange and haemolymph pH in the cockroach Nauphoeta cinerea.
The Journal of experimental biology, 2011Co-Authors: Philip G D Matthews, Craig R WhiteAbstract:Ventilatory control of internal CO(2) plays an important role in regulating extracellular acid-base balance in terrestrial animals. While this phenomenon is well understood among vertebrates, the role that respiration plays in the acid-base balance of insects is in need of much further study. To measure changes in insect haemolymph pH, we implanted micro pH optodes into the haemocoel of cockroaches (Nauphoeta cinerea). They were then exposed to normoxic, hypoxic, hyperoxic and hypercapnic atmospheres while their haemolymph pH, VCO(2) and abdominal ventilation frequency were measured simultaneously. Intratracheal O(2) levels were also measured in separate experiments. It was found that cockroaches breathing continuously control their ventilation to defend a haemolymph pH of 7.3, except under conditions where hypoxia (1% CO(2)). In contrast, intratracheal O(2) levels fluctuated widely, but on average remained above 15% in normoxic (21% O(2)) atmospheres. Decapitation caused the cockroaches to display discontinuous gas exchange cycles (DGCs). The alternating periods of ventilation and apnoea during DGCs caused haemolymph pH to fluctuate by 0.11 units. Exposure to hypoxia caused haemolymph pH to increase and initiated brief bouts of spiracular opening prior to the active ventilation phase. The spontaneous occurrence of DGCs in decapitated cockroaches indicates that central pattern generators in the thoracic and abdominal ganglia generate the periodic gas exchange pattern in the absence of control from the cephalic ganglion. This pattern continues to maintain gas exchange, but with less precision.
Chariston Andre Dal Belo - One of the best experts on this subject based on the ideXlab platform.
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entomotoxic activity of rhinella icterica spix 1824 toad skin secretion in Nauphoeta cinerea cockroaches an octopamine like modulation
Pesticide Biochemistry and Physiology, 2018Co-Authors: Allan Pinto Leal, Raquel Soares Oliveira, Patricia De Brum Vieira, Chariston Andre Dal Belo, Ana Paula Perin, Bruna Trindade Borges, Tiago Gomes Dos Santos, Lucia Vinade, Chiara ValsecchiAbstract:Abstract Rhinella icterica is a poisonous toad whose toxic secretion has never been studied against entomotoxic potential. Sublethal doses of Rhinella icterica toxic secretion (RITS) were assayed in Nauphoeta cinerea cockroaches, in order to understand the physiological and behavioral parameters, over the insect central and peripheral nervous system. RITS (10 μg/g) injections, induced behavioral impairment as evidenced by a significant decrease (38 ± 14%) in the distance traveled (p
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neurotoxicidade induzida por extrato e fracoes de manilkara rufula em baratas da especie Nauphoeta cinerea
Anais do Salão Internacional de Ensino Pesquisa e Extensão, 2018Co-Authors: Bruna Trindade Borges, Maria Eduarda Rosa, Raquel Soares Oliveira, Chariston Andre Dal Belo, Lucia Vinade, Yuri Correia Barreto, Patricia De Brum VieiraAbstract:Plantas adaptadas a regioes de clima extremo como o bioma da Caatinga possuem uma variedade de metabolitos secundarios. A Manilkara rufula e uma planta nativa dessa regiao, havendo poucos relatos sobre sua acao biologica. Buscando estudar a especie M. rufula, analises entomotoxicas foram realizadas com o objetivo de avaliar a atividade do extrato bruto e fracoes enriquecidas desta planta sobre o sistema nervoso de insetos. Para isso, baratas da especie Nauphoeta cinerea foram utilizadas como modelo experimental. O extrato bruto de M. rufula (EBMR) e suas fracoes metanolicas (FMMR) e aquosas (FAMR) foram preparadas em Milli-Q e DMSO 0,5% nas seguintes concentracoes: 25, 50 e 100 (EBMR) e 10, 20 e 40 µg/g de animal (fracoes). Tambem foram ensaiados os neuromoduladores octopamina (15 µg/g de animal) e fentolamina (0,01 µg/g de animal), um antagonista de receptores octopaminergicos. Como testes comportamentais foram realizados: ensaio de grooming e ensaio dos padroes de locomocao. Para teste eletrofisiologico foi utilizado o ensaio com registros dos potenciais de acao espontâneos sensoriais (PAES) da pata dos insetos. Houve uma diminuicao da atividade locomotora, perfil exploratorio e comportamento de grooming para todas as doses administradas do EBMR. Sendo o grooming de pernas modulado pela via octopaminergica, a octopamina induziu o aumento desse parâmetro, que foi novamente diminuido pela administracao previa do EBMR. Todas as doses do EBMR induziram um aumento da amplitude maxima do pico dos (PAES). Esse parâmetro teve uma diminuicao quando a octopamina foi administrada, porem, sofreu um aumento com a administracao previa de EBMR; semelhante a acao da fentolamina, que teve seu efeito complementado pelo EBMR. O numero de eventos (spikes) nao sofreu alteracoes pela acao isolada do EBMR, porem, quando este foi administrado previamente, reverteu o efeito da octopamina e complementou a acao da fentolamina para esse parâmetro. Ambas FAMR e FMMR (10 e 40 µg/g de animal) induziram um aumento na amplitude dos PAES. Porem, apenas a FAMR aumentou a duracao destes eventos. Considerando que a FMMR nao obteve modulacao sobre esse parâmetro, sugere-se que a FAMR seja necessaria para os efeitos do EBMR. Os dados obtidos demonstram o potencial biotecnologico da planta Manilkara rufula, bem como seu efeito entomotoxico em baratas da especie Nauphoeta cinerea. Sugere-se que o EBMR e suas fracoes possuam uma acao equivalente a antagonistas de receptores octopaminergicos, tendo em vista que os resultados obtidos com a administracao da octopamina indicam a atividade dos compostos principalmente nesta via.
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mechanism of the entomotoxic activity induced by araucaria angustifolia methanolic extract in Nauphoeta cinerea lobster cockroaches
Journal of Botany Research, 2017Co-Authors: Thiago Carrazoni, Patricia De Brum Vieira, Patricia Gomes Da Silva, Marines De Avila Heberle, Graziele Daiane Sturmer, Michele Stach Correa, Antonio Batista Pereira, Sidnei Moura, Andreas Sebastian Mendez, Chariston Andre Dal BeloAbstract:Araucaria angustifolia (Bert.) O. Kuntze (Araucariaceae), known as “Parana pine”, is a conifer found in Southern of Brazil and it needles are used traditionally as natural insecticide and acaricide. The aim of this study was to investigate the insecticide activity of A. angustifolia needles Nauphoeta cinerea as behavior model and neuromuscular preparations.
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entomotoxicidade do veneno de sapo rhinella icterica em baratas Nauphoeta cinerea
Anais do Salão Internacional de Ensino Pesquisa e Extensão, 2017Co-Authors: Yuri Correia Barreto, Raquel Soares Oliveira, Chariston Andre Dal Belo, Lucia Vinade, Allan Pinto LealAbstract:INTRODUCAO: Os sapos sao animais venenosos de interesse biotecnologico devido aos compostos bioativos presentes no seu veneno. Rhinella icterica e uma especie de anuro venenoso comum no Brasil, no qual a atividade biologica de seu veneno nunca foi descrita anteriormente. Este trabalho investigou os mecanismos da atividade entomotoxica do veneno do sapo R. icterica (VSRI). METODOLOGIA: O veneno foi extraido de sapos coletados no municipio de Derrubadas (RS), com autorizacao do Sistema de Autorizacao e Informacao em Biodiversidade (SISBIO) licenca de coletor n°: 24041-2. Baratas adultas Nauphoeta cinerea foram utilizadas como modelo experimental. A atividade neuromuscular do VSRI foi avaliada usando a preparacao musculo coxal adutor metatoracico (MCAM) segundo descrito por Martinelli et al. (2014). A Cardiotoxicidade foi avaliada utilizando a preparacao coracao semi-isolada (CSI) de barata in vivo de acordo com Rodrigues et al., (2012), frequencia cardiaca foi contabilizada visualmente durante 30 min. O comportamento de higienizacao (grooming) foi medido conforme descrito em (Carrazoni et al., 2016). Para avaliar a atividade locomotora in vivo, quatro insetos foram filmados durante 10 min e os filmes analisados utilizando softwares conforme Adedara et al. (2015). odos os dados foram expressos como media ± erro (E.P.M), sendo os mesmos analisados pelo teste two-way ANOVA, seguido dos testes Tukey ou Dunnett, sendo considerado significativos quando P
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avaliacao da influencia de neuromoduladores sobre a frequencia cardiaca de baratas da especie Nauphoeta cinerea
Anais do Salão Internacional de Ensino Pesquisa e Extensão, 2016Co-Authors: Fabiola Duarte, Lucia Vinade, Chariston Andre Dal Belo, Polyana Veloso Rodrigues, Douglas Silva Dos SantosAbstract:Os estudos com baratas tem sido motivados pelo interesse no desenvolvimento de novos agentes com atividade inseticida, bem como em ensaios de farmacologia para o desenvolvimento de novos compostos terapeuticos. O uso desse modelo tem sido estimulado pela restricao quanto ao uso de vertebrados em pesquisas cientificas bem como pelo baixo custo de criacao e manutencao desses animais em pequenos ambientes. Nesse sentido, varios neurotransmissores semelhantes aos de mamiferos ja foram identificados em insetos os quais modulam de forma direta ou indireta o sistema cardiovascular desses animais. A proposito, o sistema cardiovascular de insetos corresponde a um tubo vascular dividido em doze segmentos possuindo tanto influencia neurogenica como miogenica, pela autoritmicidade cardiaca. Recentemente, foi evidenciada a participacao do oxido nitrico sobre as respostas do sistema nervoso de insetos. O objetivo desse trabalho foi investigar a influencia de diferentes compostos (dexametasona, octopamina e atropina), sobre a modulacao da frequencia cardiaca de baratas da especie Nauphoeta cinerea usando-se a preparacao in vivo coracao semi-isolado. Nesse trabalho foram usadas baratas de ambos os sexos, adultos da especie Nauphoeta cinerea que foram alojadas em insetario com temperatura controlada (22-24C), e agua e racao a vontade. Para a montagem da preparacao as baratas foram anestesiadas por resfriamento (4C por 10min) e afixadas com alfinetes entomologicos em uma placa recoberta com isopor com a regiao ventral voltada para cima. A cuticula que recobre o abdome foi retirada com o auxilio de uma tesoura oftalmica e os orgaos viscerais removidos cuidadosamente com o auxilio de uma pinca, para a visualizacao do sistema vascular. A seguir o animal foi colocado sob uma lupa com aumento de 60X (Olympus, EUA) e os batimentos cardiacos contabilizados visualmente durante 30min. Os resultados foram expressos como a media ± EPM. Foi usado o teste t de Student como analise estatistica, onde p 0.05). Os dados sugerem que a frequencia cardiaca de baratas da especie Nauphoeta cinerea e modulada positivamente pela acetilcolina que interage com receptores muscarinicos, devido a inibicao da resposta colinergica pela atropina. A inibicao da fosfolipase A2, pelo tratamento com dexametasona, um antiinflamatorio esteroidal, e o consequente aumento da frequencia cardiaca basal na barata, sugere que as ciclooxigenases podem estar envolvidas na modulacao das respostas cronotropicas em insetos, semelhantemente ao que ocorre em mamiferos.
Joao Rocha - One of the best experts on this subject based on the ideXlab platform.
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high level of methylmercury exposure causes persisted toxicity in Nauphoeta cinerea
Environmental Science and Pollution Research, 2020Co-Authors: Bruna Candia Piccoli, Olawande C Olagoke, Michael Aschner, Jessica C Alvim, Fernanda Davila Da Silva, Pablo Andrei Nogara, Claudia S Oliveira, Joao RochaAbstract:: Methylmercury (MeHg+) is a neurotoxicant abundantly present in the environment. The long-term effects of MeHg+ have been investigated in rodents, yet data on the long-term or persisted toxicity of MeHg+ in invertebrates is scanty. Here, we examined the acute, intermediate, and chronic effects upon dietary administration of MeHg+ in nymphs of Nauphoeta cinerea. Besides, the potential reversibility of the toxic effects of MeHg+ after a detoxification period was evaluated. Nymphs were exposed to diets containing 0 (control), 2.5, 25, and 100 μg MeHg+/g of diet for 10, 30, and 90 days. Additional groups of nymphs were fed with the same dose of MeHg+ for 30 days and then were subjected to a detoxification period for 60 days. The nymphs exposed to 100 μg MeHg+/g succumbed to a high mortality rate, along with multiple biochemical (increase of reactive oxygen species production and glutathione S-transferase activity, as well as decrease in the acetylcholinesterase activity) and behavioral alterations. We observed delayed mortality rate and behavioral alterations in nymphs exposed to 100 μg MeHg+/g for 30 days and subsequently subjected to 60 days of detoxification. However, the biochemical alterations did not persist throughout the detoxification period. In conclusion, our results established the persistent toxic effect of MeHg+ even after a prolonged detoxification period and evidenced the use of N. cinerea as an alternative model to study the toxicity of MeHg+.
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staphylococcus aureus induced sepsis in the lobster cockroach Nauphoeta cinerea
Comparative Immunology Microbiology and Infectious Diseases, 2019Co-Authors: Juliana Marzari Rossato, Terimar Ruoso Moresco, Juliano Uczay, Joao RochaAbstract:Abstract Invertebrates have been instrumental in understanding the mechanisms involved in infectious diseases, considering the idea to replace, reduce and refine the use of mammals as well as to understand the basic principles of immune response in insect. We evaluated the consequences of Staphylococcus aureus-induced sepsis in the last instar nymphs of Nauphoeta cinerea injected with different concentrations of bacteria preserved in two culture media. Infected groups had a decrease in hemolymph metabolites (glucose, amino acids, total proteins, and cholesterol), in contrast to the proteins in the fat body. Higher concentrations of S. aureus caused permanent morphological alterations in adults, decrease in food consumption, increase in isolation, and increase in CFU until death of the cockroaches. Survival and protection of nymphs against a repeated and stronger challenge with the same bacteria varied according to the medium they were conserved. N. cinerea proves to be a suitable and promising model for studies related to bacterial infections.
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dietary co exposure to methylmercury and monosodium glutamate disrupts cellular and behavioral responses in the lobster cockroach Nauphoeta cinerea model
Environmental Toxicology and Pharmacology, 2018Co-Authors: Blessing A Afolabi, Isaac A Adedara, Diogo O Souza, Joao RochaAbstract:Abstract The present study aims to investigate the effect of monosodium glutamate (MSG) both separately and combined with a low dose of methylmercury (MeHg) on behavioral and biochemical parameters in Nauphoeta cinerea (lobster cockroach). Cockroaches were fed with the basal diet alone, basal diet + 2% NaCl, basal diet + 2% MSG; basal diet + 0.125 mg/g MeHg, basal diet + 0.125 mg/g MeHg + 2% NaCl; and basal diet + 0.125 mg/g MeHg + 2% MSG for 21 days. Behavioral parameters such as distance traveled, immobility and turn angle were automatically measured using ANY-maze video tracking software (Stoelting, CO, USA). Biochemical end-points such as acetylcholinesterase (AChE), glutathione-S-transferase (GST), total thiol and TBARS were also evaluated. Results show that MeHg + NaCl, increased distance traveled while MeHg + MSG increased time immobile. AChE activity was significantly reduced in cockroaches across all the groups when compared to the control. There was no significant alteration in GST activity and total thiol levels. It could be that both NaCl and MSG potentiates the neurotoxic effect of MeHg in cockroaches.
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de novo transcriptome assembly of the lobster cockroach Nauphoeta cinerea blaberidae
Genetics and Molecular Biology, 2018Co-Authors: Ana Lucia Anversa Segatto, Jose Francisco Diesel, Elgion Lucio Da Silva Loreto, Joao RochaAbstract:: The use of Drosophila as a scientific model is well established, but the use of cockroaches as experimental organisms has been increasing, mainly in toxicology research. Nauphoeta cinerea is one of the species that has been studied, and among its advantages is its easy laboratory maintenance. However, a limited amount of genetic data about N. cinerea is available, impeding gene identification and expression analyses, genetic manipulation, and a deeper understanding of its functional biology. Here we describe the N. cinerea fat body and head transcriptome, in order to provide a database of genetic sequences to better understand the metabolic role of these tissues, and describe detoxification and stress response genes. After removing low-quality sequences, we obtained 62,121 transcripts, of which more than 50% had a length of 604 pb. The assembled sequences were annotated according to their genes ontology (GO). We identified 367 genes related to stress and detoxification; among these, the more frequent were p450 genes. The results presented here are the first large-scale sequencing of N. cinerea and will facilitate the genetic understanding of the species' biochemistry processes in future works.
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neurobehavioral and biochemical changes in Nauphoeta cinerea following dietary exposure to chlorpyrifos
Pesticide Biochemistry and Physiology, 2016Co-Authors: Isaac A Adedara, Denis B Rosemberg, Diogo O Souza, Ebenezer O Farombi, Michael Aschner, Diego De Souza, Joao RochaAbstract:Abstract The present study aimed to increase our understanding about the mode of toxic action of organophosphate pesticides in insects by evaluating the biochemical and neurobehavioral characteristics in Nauphoeta cinerea exposed to chlorpyrifos (CPF)-contaminated diet. The insects were exposed for 35 consecutive days to CPF at 0.078, 0.15625, 0.3125 and 0.625 μg/g feed. Locomotor behavior was assessed for a 10-min trial in a novel arena and subsequently, biochemical analyses were carried out using the cockroaches’ heads. In comparison to control, CPF-exposed cockroaches showed significant decreases in the total distance traveled, body rotation, turn angle and meandering, along with significant increase in the number of falls, time and episodes of immobility. The marked decrease in the exploratory profiles of CPF-exposed cockroaches was confirmed by track plots, whereas occupancy plot analyses showed a progressive dispersion at 0.15625 μg/g feed group. Moreover, the heads of CPF-exposed cockroaches showed marked decrease in acetylcholinesterase activity and antioxidant status with concomitant significant elevation in dichlorofluorescein oxidation and lipid peroxidation levels in CPF-treated cockroaches. Gas Chromatography–Mass Spectrometry analyses revealed bioaccumulation of CPF in cockroaches exposed to concentrations above 0.078 μg/g feed. The findings from this investigation showed N. cinerea as a value model organism for the risk assessment of environmental organophosphate contamination in insects.
Allan Pinto Leal - One of the best experts on this subject based on the ideXlab platform.
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neurotoxic effects of sublethal concentrations of cyanobacterial extract containing anatoxin a s on Nauphoeta cinerea cockroaches
Ecotoxicology and Environmental Safety, 2019Co-Authors: Douglas Silva Dos Santos, Maria Eduarda Rosa, Ana Paula Zanatta, Raquel Soares Oliveira, Carlos Gabriel Moreira De Almeida, Allan Pinto Leal, Miriam SanzAbstract:Abstract The detection of cyanotoxins, such as the anatoxin-a(s), is essential to ensure the biological safety of water environments. Here, we propose the use of Nauphoeta cinerea cockroaches as an alternative biological model for the biomonitoring of the activity of anatoxin-a(s) in aquatic systems. In order to validate our proposed model, we compared the effects of a cyanobacterial extract containing anatoxin-a(s) (CECA) with those of the organophosphate trichlorfon (Tn) on biochemical and physiological parameters of the nervous system of Nauphoeta cinerea cockroaches. In brain homogenates from cockroaches, CECA (5 and 50 μg/g) inhibited acetylcholinesterase (AChE) activity by 53 ± 2% and 51 ± 7%, respectively, while Tn (5 and 50 μg/g) inhibited AChE activity by 35 ± 4% and 80 ± 9%, respectively (p 0.05). Finally, CECA (50 µg/g), Tn (0.3 µg/g) and neostigmine (50 µg/g) caused significant neuromuscular failure, as indicated by the monitoring of the in vivo neuromuscular function of the cockroaches, during 100 min (n = 6, p
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entomotoxic activity of rhinella icterica spix 1824 toad skin secretion in Nauphoeta cinerea cockroaches an octopamine like modulation
Pesticide Biochemistry and Physiology, 2018Co-Authors: Allan Pinto Leal, Raquel Soares Oliveira, Patricia De Brum Vieira, Chariston Andre Dal Belo, Ana Paula Perin, Bruna Trindade Borges, Tiago Gomes Dos Santos, Lucia Vinade, Chiara ValsecchiAbstract:Abstract Rhinella icterica is a poisonous toad whose toxic secretion has never been studied against entomotoxic potential. Sublethal doses of Rhinella icterica toxic secretion (RITS) were assayed in Nauphoeta cinerea cockroaches, in order to understand the physiological and behavioral parameters, over the insect central and peripheral nervous system. RITS (10 μg/g) injections, induced behavioral impairment as evidenced by a significant decrease (38 ± 14%) in the distance traveled (p
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entomotoxicidade do veneno de sapo rhinella icterica em baratas Nauphoeta cinerea
Anais do Salão Internacional de Ensino Pesquisa e Extensão, 2017Co-Authors: Yuri Correia Barreto, Raquel Soares Oliveira, Chariston Andre Dal Belo, Lucia Vinade, Allan Pinto LealAbstract:INTRODUCAO: Os sapos sao animais venenosos de interesse biotecnologico devido aos compostos bioativos presentes no seu veneno. Rhinella icterica e uma especie de anuro venenoso comum no Brasil, no qual a atividade biologica de seu veneno nunca foi descrita anteriormente. Este trabalho investigou os mecanismos da atividade entomotoxica do veneno do sapo R. icterica (VSRI). METODOLOGIA: O veneno foi extraido de sapos coletados no municipio de Derrubadas (RS), com autorizacao do Sistema de Autorizacao e Informacao em Biodiversidade (SISBIO) licenca de coletor n°: 24041-2. Baratas adultas Nauphoeta cinerea foram utilizadas como modelo experimental. A atividade neuromuscular do VSRI foi avaliada usando a preparacao musculo coxal adutor metatoracico (MCAM) segundo descrito por Martinelli et al. (2014). A Cardiotoxicidade foi avaliada utilizando a preparacao coracao semi-isolada (CSI) de barata in vivo de acordo com Rodrigues et al., (2012), frequencia cardiaca foi contabilizada visualmente durante 30 min. O comportamento de higienizacao (grooming) foi medido conforme descrito em (Carrazoni et al., 2016). Para avaliar a atividade locomotora in vivo, quatro insetos foram filmados durante 10 min e os filmes analisados utilizando softwares conforme Adedara et al. (2015). odos os dados foram expressos como media ± erro (E.P.M), sendo os mesmos analisados pelo teste two-way ANOVA, seguido dos testes Tukey ou Dunnett, sendo considerado significativos quando P
