The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
P B Mckenna - One of the best experts on this subject based on the ideXlab platform.
-
the effect of previous cold storage on the subsequent recovery of infective third stage Nematode Larvae from sheep faeces
Veterinary Parasitology, 1998Co-Authors: P B MckennaAbstract:Abstract An investigation undertaken to determine the effect of previous cold storage on the recovery of third stage Larvae of gastrointestinal Nematodes of sheep, showed that increasing periods of exposure of faeces to 4°C resulted in decreasing numbers of Larvae subsequently recovered from them. Differences in the abilities of the eggs of the various genera, to survive such treatment, were found to lead to significant changes in the percentage generic compositions of their third stage Larvae – in some cases following the prior refrigeration of faecal samples for as little as 24 h. These results suggest that where larval cultures are intended to provide estimates of the proportions of the various worm eggs in the faeces of sheep harboring mixed gastrointestinal Nematode burdens, they should be performed only on freshly collected samples.
-
the effect of previous cold storage on the subsequent recovery of infective third stage Nematode Larvae from sheep faeces
Veterinary Parasitology, 1998Co-Authors: P B MckennaAbstract:An investigation undertaken to determine the effect of previous cold storage on the recovery of third stage Larvae of gastrointestinal Nematodes of sheep, showed that increasing periods of exposure of faeces to 4 degrees C resulted in decreasing numbers of Larvae subsequently recovered from them. Differences in the abilities of the eggs of the various genera, to survive such treatment, were found to lead to significant changes in the percentage generic compositions of their third stage Larvae--in some cases following the prior refrigeration of faecal samples for as little as 24 h. These results suggest that where larval cultures are intended to provide estimates of the proportions of the various worm eggs in the faeces of sheep harboring mixed gastrointestinal Nematode burdens, they should be performed only on freshly collected samples.
Juliana Novo Borges - One of the best experts on this subject based on the ideXlab platform.
-
morphological and molecular diagnosis of anisakid Nematode Larvae from cutlassfish trichiurus lepturus off the coast of rio de janeiro brazil
PLOS ONE, 2012Co-Authors: Juliana Novo Borges, Luiz Felipe Gullo Cunha, Helena Santos, Cassiano Monteironeto, Claudia Portes SantosAbstract:Anisakid Nematode Larvae from Trichiurus lepturus off coast of Rio de Janeiro were studied using light, laser confocal and scanning electron microscopy, in addition to a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), partial 28S (LSU) and internal transcribed spacers (ITS-1, 5.8S, ITS-2) of ribosomal DNA were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships between the Nematode taxa. The morphological and genetic profiles confirmed that, of the 1,030 Larvae collected from the 64 fish examined, 398 were analysed, of which 361 were Hysterothylacium sp. and 37 were Anisakis typica. Larvae of Hysterothylacium sp. were not identified to the species level due to the absence of similar sequences for adult parasites; however, the ITS sequence clustered in the phylogenetic tree with sequences of H. deardorffoverstreetorum, whereas an mtDNA cox-2 and LSU concatenated phylogenetic analysis demonstrated the presence of two clades, both of them under the same name as the larval H. deardorffoverstreetorum. Data on the occurrence of parasites during the winter and summer months were compared using the t-test. The greatest prevalence and intensity of infection were recorded for larval Hysterothylacium, with a prevalence of 51.56% and an intensity of up to 55 parasites per fish. The larval Anisakis exhibit a higher abundance and intensity of infection in the winter months, and those of Hysterothylacium during the summer. However, the t-test indicated no significant differences between the abundance and intensity of infection recorded during the months of collection for either of these larval Nematodes. All sequences generated in this study were deposited in GenBank.
Shokoofeh Shamsi - One of the best experts on this subject based on the ideXlab platform.
-
occurrence of contracaecum bancrofti Larvae in fish in the murray darling basin
Journal of Helminthology, 2019Co-Authors: Shokoofeh Shamsi, A. Stoddart, Lesley R. Smales, Skye WassensAbstract:There is a paucity of information on the diversity and occurrence of freshwater fish parasites in Australia. This study investigates the distribution and occurrence of a parasitic Nematode of the genus Contracaecum in freshwater fish from south-eastern Australia. Fish ( n = 508) belonging to nine species and eight families were collected from eight wetlands associated with the Murrumbidgee River floodplain in the southern Murray–Darling Basin and subjected to laboratory examination. Third-stage Nematode Larvae were found in eight of the nine fish species. The exception was the fly-specked hardeyhead ( Craterocephalus stercusmuscarum ), although only one specimen of this species was examined. Nematode Larvae were identified as Contracaecum bancrofti using a combined morphological and molecular approach. The parasite was most prevalent in weather loach ( Misgurnus anguillicaudatus ; 34.8%) and Australian smelt ( Retropinna semoni ; 21.4%), followed by carp ( Cyprinus carpio ; 9.5%), of which the former and latter are non-native species. Contracaecum prevalence differed between locations, with Two Bridges having the highest number of infected fish. This may be due to the higher number of suitable host species collected at these localities. Contracaecum spp. are parasites with low host specificity that have also been reported in Australian marine fish, humans and piscivorous birds. The high parasite prevalence in the two abundant non-native fish species in the region suggests that they act as a suitable host for this endemic parasite, resulting in the increase in the parasite population. It would be interesting to study host–parasite interactions in this area, especially if introduced fish populations declined dramatically in the attempt to eradicate them or control their population.
-
new and known zoonotic Nematode Larvae within selected fish species from queensland waters in australia
International Journal of Food Microbiology, 2018Co-Authors: Shokoofeh Shamsi, Eleanor Steller, Yuchi ChenAbstract:Abstract Zoonotic fish-borne Nematodes are a significant and growing global health concern. However, in Australia their occurrence and geographical distribution are not well known. Moreton Bay in Queensland, Australia, is a popular tourist destination for domestic and international visitors where seafood is a popular food item. The aim of this study was to determine the occurrence of zoonotic Nematodes within four commercially significant teleost fish species from Moreton Bay; Surf Bream, Sea Mullet, Yellow-Fin Tripod fish and Gold-Lined Sea Bream. Overall, 34.03% of fish examined (n = 144) were found to be a host to at least one parasitic Nematode. The seasonal dynamics and prevalence of Nematode infection was tested by statistical means. Results indicated a significant difference in seasonal infection for Surf Bream (p
-
a revised method of examining fish for infection with zoonotic Nematode Larvae
International Journal of Food Microbiology, 2016Co-Authors: Shokoofeh Shamsi, Jaydipbhai SutharAbstract:The infection of fish with zoonotic Nematodes, particularly anisakid Nematodes is of great interest to many researchers who study food safety, human or animal health or who use them as biological tags for stock assessment studies. Accurate examination of fish for infection with anisakid Larvae is crucial in making accurate estimates of their occurrence, abundance and prevalence in their fish hosts. Here we describe a new method of examining fish for infection with these parasites. In 2015, a total of 261 fish were purchased from a fish market in New South Wales, Australia. All fish were first examined by routine visual examination for infection with zoonotic Nematode Larvae and all data were recorded. Subsequently all internal organs were placed in a container and filled with water and incubated in the room temperature overnight. The prevalence, mean intensity and mean abundance of anisakids were significantly higher (p<0.05) when the revised method of examination, i.e., combining visual examination and overnight incubation in room temperature, was employed (63.98, 8.23 and 5.27, respectively) compared to routine visual examination with or without the aid of a microscope (8.81, 3.78 and 0.33, respectively). The proposed method is effective and has several advantages, such as: not using UV or HCl for fish examination, allowing the examination of a larger number of fish in shorter time; larval specimens collected being suitable for both morphological and DNA sequencing; and being simple and inexpensive. The disadvantages would be the odour of the specimens after overnight incubation as well as not being suitable for use with frozen fish. We suggest that results, conclusions or recommendations made in studies that claim no anisakid/ascaridoid Larvae were found in a fish should be approached carefully if it is only based on visual examination of the fish.
-
occurrence and abundance of anisakid Nematode Larvae in five species of fish from southern australian waters
Parasitology Research, 2011Co-Authors: Shokoofeh Shamsi, Robin B Gasser, Ian Beveridge, Albert Eisenbarth, Shruti R Saptarshi, Andreas L LopataAbstract:The aim of the present study was to conduct, in southern Australian waters, a preliminary epidemiological survey of five commercially significant species of fish (yellow-eye mullet, tiger flathead, sand flathead, pilchard and king fish) for infections with anisakid Nematodes Larvae using a combined morphological–molecular approach. With the exception of king fish, which was farmed and fed commercial pellets, all other species were infected with at least one species of anisakid Nematode, with each individual tiger flathead examined being infected. Five morphotypes, including Anisakis, Contracaecum type I and II and Hysterothylacium type IV and VIII, were defined genetically using mutation scanning and targeted sequencing of the second internal transcribed spacer of nuclear ribosomal DNA. The findings of the present study provide a basis for future investigations of the genetic composition of anisakid populations in a wide range of fish hosts in Australia and for assessing their public health significance.
Andreas L Lopata - One of the best experts on this subject based on the ideXlab platform.
-
molecular characterization of anisakid Nematode Larvae from 13 species of fish from western australia
International Journal of Food Microbiology, 2013Co-Authors: Abdul Jabbar, Rachel W J Fong, Kia X Kok, Andreas L Lopata, Robin B Gasser, Ian BeveridgeAbstract:article i nfo This study characterized anisakid Nematodes in estuarine and near-shore species of fish in southern Western Australia. A total of 108 fish representing 13 species were examined for anisakid Larvae. For the molecular char- acterization of anisakid Larvae (n=218), we used PCR-coupled mutation scanning-sequencing-phylogenetic analyses of sequence variation in the internal transcribed spacers of nuclear ribosomal DNA. With the exception of Sillaginoides punctatus and Sillago schomburgkii, all the fish species examined (Aldrichetta forsteri, Arripis georgianus, Hyporhamphus regularis, Mugil cephalus, Platycephalus speculator, Pomatomus saltatrix, Pseudocaranx dentex, Pseudocaranx wrighti, Thysanophrys cirronatus, Trachurus novaezeelandiae and Upeneichthys lineatus) harboured at least one species of anisakid. Mutation scanning analysis identified 11 different genotypes of anisakid Larvae. Phylogenetic analyses of the sequence data, employing reference sequence data for a wide range of anisakids (31 species) from public databases, revealed the presence of Anisakis pegreffii (n=3), Contracaecum multipapillatum (49), Contracaecum ogmorhini (1), Hysterothylacium larval type IV (82), Hysterothylacium larval type Vb (14), Hysterothylacium larval type VIII (3), Hysterothylacium larval type X (65), and Terranova type I (1) in the fish examined. The present study provides valuable information on the diversity of anisakids in southern Western Australia and also a basis for future investigations to assess the public health significance of these parasites.
-
occurrence and abundance of anisakid Nematode Larvae in five species of fish from southern australian waters
Parasitology Research, 2011Co-Authors: Shokoofeh Shamsi, Robin B Gasser, Ian Beveridge, Albert Eisenbarth, Shruti R Saptarshi, Andreas L LopataAbstract:The aim of the present study was to conduct, in southern Australian waters, a preliminary epidemiological survey of five commercially significant species of fish (yellow-eye mullet, tiger flathead, sand flathead, pilchard and king fish) for infections with anisakid Nematodes Larvae using a combined morphological–molecular approach. With the exception of king fish, which was farmed and fed commercial pellets, all other species were infected with at least one species of anisakid Nematode, with each individual tiger flathead examined being infected. Five morphotypes, including Anisakis, Contracaecum type I and II and Hysterothylacium type IV and VIII, were defined genetically using mutation scanning and targeted sequencing of the second internal transcribed spacer of nuclear ribosomal DNA. The findings of the present study provide a basis for future investigations of the genetic composition of anisakid populations in a wide range of fish hosts in Australia and for assessing their public health significance.
Claudia Portes Santos - One of the best experts on this subject based on the ideXlab platform.
-
morphological and molecular diagnosis of anisakid Nematode Larvae from cutlassfish trichiurus lepturus off the coast of rio de janeiro brazil
PLOS ONE, 2012Co-Authors: Juliana Novo Borges, Luiz Felipe Gullo Cunha, Helena Santos, Cassiano Monteironeto, Claudia Portes SantosAbstract:Anisakid Nematode Larvae from Trichiurus lepturus off coast of Rio de Janeiro were studied using light, laser confocal and scanning electron microscopy, in addition to a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), partial 28S (LSU) and internal transcribed spacers (ITS-1, 5.8S, ITS-2) of ribosomal DNA were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships between the Nematode taxa. The morphological and genetic profiles confirmed that, of the 1,030 Larvae collected from the 64 fish examined, 398 were analysed, of which 361 were Hysterothylacium sp. and 37 were Anisakis typica. Larvae of Hysterothylacium sp. were not identified to the species level due to the absence of similar sequences for adult parasites; however, the ITS sequence clustered in the phylogenetic tree with sequences of H. deardorffoverstreetorum, whereas an mtDNA cox-2 and LSU concatenated phylogenetic analysis demonstrated the presence of two clades, both of them under the same name as the larval H. deardorffoverstreetorum. Data on the occurrence of parasites during the winter and summer months were compared using the t-test. The greatest prevalence and intensity of infection were recorded for larval Hysterothylacium, with a prevalence of 51.56% and an intensity of up to 55 parasites per fish. The larval Anisakis exhibit a higher abundance and intensity of infection in the winter months, and those of Hysterothylacium during the summer. However, the t-test indicated no significant differences between the abundance and intensity of infection recorded during the months of collection for either of these larval Nematodes. All sequences generated in this study were deposited in GenBank.
