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Nicole L Snyder - One of the best experts on this subject based on the ideXlab platform.
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Synthesis of isobemisiose, neosartose, and fischerose: three α-1,6-linked trehalose-based oligosaccharides identified from Neosartorya fischeri
RSC Advances, 2020Co-Authors: Eric J Kuenstner, E. A. Palumbo, J. Levine, Nicole L SnyderAbstract:Three complex α-1,6-linked trehalose-based oligosaccharides with unique preservation properties, isobemisiose, neosartose, and fischerose, were recently identified from the extreme stress-tolerant ascospores of Neosartorya fischeri. Herein, we report the first concise, scalable, and iterative chemical synthesis of these oligosaccharides from a differentially protected thioglycoside donor and a selectively protected, asymmetric trehalose acceptor. This work constitutes an improved synthesis of isobemisiose, and is also the first reported synthesis of neosartose, a tetrasaccharide, and fischerose, a pentasaccharide, in good yield. Importantly, in-depth studies of biological function are enabled by this synthetic platform.
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Functionality and prevalence of trehalose‐based oligosaccharides as novel compatible solutes in ascospores of Neosartorya fischeri (Aspergillus fischeri) and other fungi
Environmental Microbiology, 2014Co-Authors: Timon T Wyatt, M. Richard Van Leeuwen, Elena A. Golovina, Edward A. Palumbo, Alex E.e. Verkennis, Nicole L Snyder, Folkert A. Hoekstra, Eric J Kuenstner, C.m. Visagie, John E. HallsworthAbstract:Ascospores of Neosartorya, Byssochlamys and Talaromyces can be regarded as the most stress-resistant eukaryotic cells. They can survive exposure at temperatures as high as 85°C for 100 min or more. Neosartorya fischeri ascospores are more viscous and more resistant to the combined stress of heat and desiccation than the ascospores of Talaromyces macrosporus which contain predominantly trehalose. These ascospores contain trehalose-based oligosaccharides (TOS) that are novel compatible solutes, which are accumulated to high levels. These compounds are also found in other members of the genus Neosartorya and in some other genera within the order Eurotiales that also include Byssochlamys and Talaromyces. The presence of oligosaccharides was observed in species that had a relatively high growth temperature. TOS glasses have a higher glass transition temperature (Tg ) than trehalose, and they form a stable glass with crystallizing molecules, such as mannitol. Our data indicate that TOS are important for prolonged stabilization of cells against stress. The possible unique role of these solutes in protection against dry heat conditions is discussed.
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Functionality and prevalence of trehalose-based oligosaccharides as novel compatible solutes in ascospores of Neosartorya fischeri (Aspergillus fischeri) and other fungi
Environmental microbiology, 2014Co-Authors: Timon T Wyatt, Elena A. Golovina, Edward A. Palumbo, Alex E.e. Verkennis, Nicole L Snyder, Folkert A. Hoekstra, Eric J Kuenstner, C.m. Visagie, M. Richard Van Leeuwen, John E. HallsworthAbstract:Ascospores of Neosartorya, Byssochlamys and Talaromyces can be regarded as the most stress-resistant eukaryotic cells. They can survive exposure at temperatures as high as 85°C for 100 min or more. Neosartorya fischeri ascospores are more viscous and more resistant to the combined stress of heat and desiccation than the ascospores of Talaromyces macrosporus which contain predominantly trehalose. These ascospores contain trehalose-based oligosaccharides (TOS) that are novel compatible solutes, which are accumulated to high levels. These compounds are also found in other members of the genus Neosartorya and in some other genera within the order Eurotiales that also include Byssochlamys and Talaromyces. The presence of oligosaccharides was observed in species that had a relatively high growth temperature. TOS glasses have a higher glass transition temperature (Tg ) than trehalose, and they form a stable glass with crystallizing molecules, such as mannitol. Our data indicate that TOS are important for prolonged stabilization of cells against stress. The possible unique role of these solutes in protection against dry heat conditions is discussed.
Robert A Samson - One of the best experts on this subject based on the ideXlab platform.
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new taxa of Neosartorya and aspergillus in aspergillus section fumigati
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2008Co-Authors: Seung-beom Hong, Hyeon-dong Shin, Jens Christian Frisvad, János Varga, Joonbae Hong, Per Vaeggemose Nielsen, Robert A SamsonAbstract:Three new species of Neosartorya and one new Aspergillus of section Fumigati are proposed using a polyphasic approach based on morphology, extrolite production and partial β-tubulin, calmodulin, and actin gene sequences. The phylogenetic analyses using the three genes clearly show that the taxa grouped separately from the known species and confirmed the phenotypic differences. Neosartorya denticulata is characterized by its unique denticulate ascospores with a prominent equatorial furrow; N. assulata by well developed flaps on the convex surface of the ascospores which in addition have two distinct equatorial crests and N. galapagensis by a funiculose colony morphology, short and narrow conidiophores and ascospores with two wide equatorial crests with a microtuberculate convex surface. Aspergillus turcosus can be distinguished by velvety, gray turquoise colonies and short, loosely columnar conidial heads. The four new taxa also have unique extrolite profiles, which contain the mycotoxins gliotoxin and viriditoxin in N. denticulate; apolar compounds provisionally named NEPS in N. assulata and gregatins in N. galapagensis. A. turcosus produced kotanins. N. denticulata sp. nov., N. assulata sp. nov., N. galapagensis sp. nov., and A. turcosus sp. nov. are described and illustrated.
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New taxa of Neosartorya and Aspergillus in Aspergillus section Fumigati.
Antonie van Leeuwenhoek, 2007Co-Authors: Seung-beom Hong, Hyeon-dong Shin, János Varga, Joonbae Hong, Per Vaeggemose Nielsen, Jens C Frisvad, Robert A SamsonAbstract:Three new species of Neosartorya and one new Aspergillus of section Fumigati are proposed using a polyphasic approach based on morphology, extrolite production and partial beta-tubulin, calmodulin, and actin gene sequences. The phylogenetic analyses using the three genes clearly show that the taxa grouped separately from the known species and confirmed the phenotypic differences. Neosartorya denticulata is characterized by its unique denticulate ascospores with a prominent equatorial furrow; N. assulata by well developed flaps on the convex surface of the ascospores which in addition have two distinct equatorial crests and N. galapagensis by a funiculose colony morphology, short and narrow conidiophores and ascospores with two wide equatorial crests with a microtuberculate convex surface. Aspergillus turcosus can be distinguished by velvety, gray turquoise colonies and short, loosely columnar conidial heads. The four new taxa also have unique extrolite profiles, which contain the mycotoxins gliotoxin and viriditoxin in N. denticulate; apolar compounds provisionally named NEPS in N. assulata and gregatins in N. galapagensis. A. turcosus produced kotanins. N. denticulata sp. nov., N. assulata sp. nov., N. galapagensis sp. nov., and A. turcosus sp. nov. are described and illustrated.
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Novel Neosartorya species isolated from soil in Korea.
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Seung-beom Hong, Hye Sun Cho, Hyeon-dong Shin, Jens Christian Frisvad, Robert A SamsonAbstract:Phenotypic and genotypic characters of strains of Neosartorya spinosa and related taxa were analysed. N. spinosa, Neosartorya botucatensis and Neosartorya paulistensis had identical partial β-tubulin and calmodulin gene sequences and could not be differentiated on macro- and micro-morphological characteristics, including by scanning electron microscopy. Based on partial β-tubulin and calmodulin gene sequences and ascospore morphology, two separate groups are distinguished and are proposed as novel species. Neosartorya laciniosa sp. nov. has microtuberculate ascospores with two bent crests and two distinct equatorial rings of small projections. Neosartorya coreana sp. nov. has rugose to weak reticulate ascospores with two often bent crests, but without the equatorial rings of small projections. The type strain of N. laciniosa is CBS 117721T (=NRRL 35589T=KACC 41657T) and the type strain of N. coreana is CBS 117059T (=NRRL 35590T=KACC 41659T).
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Novel Neosartorya species isolated from soil in Korea.
International journal of systematic and evolutionary microbiology, 2006Co-Authors: Seung-beom Hong, Hye Sun Cho, Hyeon-dong Shin, Jens Christian Frisvad, Robert A SamsonAbstract:Phenotypic and genotypic characters of strains of Neosartorya spinosa and related taxa were analysed. N. spinosa, Neosartorya botucatensis and Neosartorya paulistensis had identical partial beta-tubulin and calmodulin gene sequences and could not be differentiated on macro- and micro-morphological characteristics, including by scanning electron microscopy. Based on partial beta-tubulin and calmodulin gene sequences and ascospore morphology, two separate groups are distinguished and are proposed as novel species. Neosartorya laciniosa sp. nov. has microtuberculate ascospores with two bent crests and two distinct equatorial rings of small projections. Neosartorya coreana sp. nov. has rugose to weak reticulate ascospores with two often bent crests, but without the equatorial rings of small projections. The type strain of N. laciniosa is CBS 117721T (=NRRL 35589T=KACC 41657T) and the type strain of N. coreana is CBS 117059T (=NRRL 35590T=KACC 41659T).
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Isolation of Neosartorya pseudofischeri from Blood: First Hint of Pulmonary Aspergillosis
Journal of clinical microbiology, 2004Co-Authors: H. Järv, Robert A Samson, Richard C. Summerbell, J. Lehtmaa, E. S. Hoekstra, P. NaaberAbstract:We report a case of invasive pulmonary aspergillosis caused by Neosartorya pseudofischeri S. W. Peterson [anamorph Aspergillus thermomutatus (Paden) S. W. Peterson]. The diagnosis was initially based on a positive blood culture for a strain isolated from a neutropenic patient by means of a BACTEC 9050 blood culture system. The final diagnosis was established based on X-ray and computer tomography scan results as well as the detection of Aspergillus antigen in the patient's serum.
Timon T Wyatt - One of the best experts on this subject based on the ideXlab platform.
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Structural analysis of novel trehalose-based oligosaccharides from extremely stress-tolerant ascospores of Neosartorya fischeri (Aspergillus fischeri)
Carbohydrate research, 2015Co-Authors: Timon T Wyatt, Gerrit J. Gerwig, Johannis P. Kamerling, Han A. B. Wösten, Jan DijksterhuisAbstract:Different fungi, including the genera Neosartorya, Byssochlamys and Talaromyces, produce (asco)spores that survive pasteurization treatments and are regarded as the most stress-resistant eukaryotic cells. Here, the NMR analysis of a series of trehalose-based oligosaccharides, being compatible solutes that are accumulated to high levels in ascospores of the fungus Neosartorya fischeri, is presented. These oligosaccharides consist of an α,α-trehalose backbone, extended with one [α-D-Glcp-(1 → 6)-α-D-Glcp-(1 ↔ 1)-α-D-Glcp; isobemisiose], two [α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 ↔ 1)-α-D-Glcp] or three [α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 → 6)-α-D-Glcp-(1 ↔ 1)-α-D-Glcp] glucose units. The tetra- and pentasaccharide, dubbed neosartose and fischerose, respectively, have not been reported before to occur in nature.
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Functionality and prevalence of trehalose‐based oligosaccharides as novel compatible solutes in ascospores of Neosartorya fischeri (Aspergillus fischeri) and other fungi
Environmental Microbiology, 2014Co-Authors: Timon T Wyatt, M. Richard Van Leeuwen, Elena A. Golovina, Edward A. Palumbo, Alex E.e. Verkennis, Nicole L Snyder, Folkert A. Hoekstra, Eric J Kuenstner, C.m. Visagie, John E. HallsworthAbstract:Ascospores of Neosartorya, Byssochlamys and Talaromyces can be regarded as the most stress-resistant eukaryotic cells. They can survive exposure at temperatures as high as 85°C for 100 min or more. Neosartorya fischeri ascospores are more viscous and more resistant to the combined stress of heat and desiccation than the ascospores of Talaromyces macrosporus which contain predominantly trehalose. These ascospores contain trehalose-based oligosaccharides (TOS) that are novel compatible solutes, which are accumulated to high levels. These compounds are also found in other members of the genus Neosartorya and in some other genera within the order Eurotiales that also include Byssochlamys and Talaromyces. The presence of oligosaccharides was observed in species that had a relatively high growth temperature. TOS glasses have a higher glass transition temperature (Tg ) than trehalose, and they form a stable glass with crystallizing molecules, such as mannitol. Our data indicate that TOS are important for prolonged stabilization of cells against stress. The possible unique role of these solutes in protection against dry heat conditions is discussed.
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Functionality and prevalence of trehalose-based oligosaccharides as novel compatible solutes in ascospores of Neosartorya fischeri (Aspergillus fischeri) and other fungi
Environmental microbiology, 2014Co-Authors: Timon T Wyatt, Elena A. Golovina, Edward A. Palumbo, Alex E.e. Verkennis, Nicole L Snyder, Folkert A. Hoekstra, Eric J Kuenstner, C.m. Visagie, M. Richard Van Leeuwen, John E. HallsworthAbstract:Ascospores of Neosartorya, Byssochlamys and Talaromyces can be regarded as the most stress-resistant eukaryotic cells. They can survive exposure at temperatures as high as 85°C for 100 min or more. Neosartorya fischeri ascospores are more viscous and more resistant to the combined stress of heat and desiccation than the ascospores of Talaromyces macrosporus which contain predominantly trehalose. These ascospores contain trehalose-based oligosaccharides (TOS) that are novel compatible solutes, which are accumulated to high levels. These compounds are also found in other members of the genus Neosartorya and in some other genera within the order Eurotiales that also include Byssochlamys and Talaromyces. The presence of oligosaccharides was observed in species that had a relatively high growth temperature. TOS glasses have a higher glass transition temperature (Tg ) than trehalose, and they form a stable glass with crystallizing molecules, such as mannitol. Our data indicate that TOS are important for prolonged stabilization of cells against stress. The possible unique role of these solutes in protection against dry heat conditions is discussed.
John E. Hallsworth - One of the best experts on this subject based on the ideXlab platform.
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Functionality and prevalence of trehalose‐based oligosaccharides as novel compatible solutes in ascospores of Neosartorya fischeri (Aspergillus fischeri) and other fungi
Environmental Microbiology, 2014Co-Authors: Timon T Wyatt, M. Richard Van Leeuwen, Elena A. Golovina, Edward A. Palumbo, Alex E.e. Verkennis, Nicole L Snyder, Folkert A. Hoekstra, Eric J Kuenstner, C.m. Visagie, John E. HallsworthAbstract:Ascospores of Neosartorya, Byssochlamys and Talaromyces can be regarded as the most stress-resistant eukaryotic cells. They can survive exposure at temperatures as high as 85°C for 100 min or more. Neosartorya fischeri ascospores are more viscous and more resistant to the combined stress of heat and desiccation than the ascospores of Talaromyces macrosporus which contain predominantly trehalose. These ascospores contain trehalose-based oligosaccharides (TOS) that are novel compatible solutes, which are accumulated to high levels. These compounds are also found in other members of the genus Neosartorya and in some other genera within the order Eurotiales that also include Byssochlamys and Talaromyces. The presence of oligosaccharides was observed in species that had a relatively high growth temperature. TOS glasses have a higher glass transition temperature (Tg ) than trehalose, and they form a stable glass with crystallizing molecules, such as mannitol. Our data indicate that TOS are important for prolonged stabilization of cells against stress. The possible unique role of these solutes in protection against dry heat conditions is discussed.
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Functionality and prevalence of trehalose-based oligosaccharides as novel compatible solutes in ascospores of Neosartorya fischeri (Aspergillus fischeri) and other fungi
Environmental microbiology, 2014Co-Authors: Timon T Wyatt, Elena A. Golovina, Edward A. Palumbo, Alex E.e. Verkennis, Nicole L Snyder, Folkert A. Hoekstra, Eric J Kuenstner, C.m. Visagie, M. Richard Van Leeuwen, John E. HallsworthAbstract:Ascospores of Neosartorya, Byssochlamys and Talaromyces can be regarded as the most stress-resistant eukaryotic cells. They can survive exposure at temperatures as high as 85°C for 100 min or more. Neosartorya fischeri ascospores are more viscous and more resistant to the combined stress of heat and desiccation than the ascospores of Talaromyces macrosporus which contain predominantly trehalose. These ascospores contain trehalose-based oligosaccharides (TOS) that are novel compatible solutes, which are accumulated to high levels. These compounds are also found in other members of the genus Neosartorya and in some other genera within the order Eurotiales that also include Byssochlamys and Talaromyces. The presence of oligosaccharides was observed in species that had a relatively high growth temperature. TOS glasses have a higher glass transition temperature (Tg ) than trehalose, and they form a stable glass with crystallizing molecules, such as mannitol. Our data indicate that TOS are important for prolonged stabilization of cells against stress. The possible unique role of these solutes in protection against dry heat conditions is discussed.
Eric J Kuenstner - One of the best experts on this subject based on the ideXlab platform.
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Synthesis of isobemisiose, neosartose, and fischerose: three α-1,6-linked trehalose-based oligosaccharides identified from Neosartorya fischeri
RSC Advances, 2020Co-Authors: Eric J Kuenstner, E. A. Palumbo, J. Levine, Nicole L SnyderAbstract:Three complex α-1,6-linked trehalose-based oligosaccharides with unique preservation properties, isobemisiose, neosartose, and fischerose, were recently identified from the extreme stress-tolerant ascospores of Neosartorya fischeri. Herein, we report the first concise, scalable, and iterative chemical synthesis of these oligosaccharides from a differentially protected thioglycoside donor and a selectively protected, asymmetric trehalose acceptor. This work constitutes an improved synthesis of isobemisiose, and is also the first reported synthesis of neosartose, a tetrasaccharide, and fischerose, a pentasaccharide, in good yield. Importantly, in-depth studies of biological function are enabled by this synthetic platform.
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Functionality and prevalence of trehalose‐based oligosaccharides as novel compatible solutes in ascospores of Neosartorya fischeri (Aspergillus fischeri) and other fungi
Environmental Microbiology, 2014Co-Authors: Timon T Wyatt, M. Richard Van Leeuwen, Elena A. Golovina, Edward A. Palumbo, Alex E.e. Verkennis, Nicole L Snyder, Folkert A. Hoekstra, Eric J Kuenstner, C.m. Visagie, John E. HallsworthAbstract:Ascospores of Neosartorya, Byssochlamys and Talaromyces can be regarded as the most stress-resistant eukaryotic cells. They can survive exposure at temperatures as high as 85°C for 100 min or more. Neosartorya fischeri ascospores are more viscous and more resistant to the combined stress of heat and desiccation than the ascospores of Talaromyces macrosporus which contain predominantly trehalose. These ascospores contain trehalose-based oligosaccharides (TOS) that are novel compatible solutes, which are accumulated to high levels. These compounds are also found in other members of the genus Neosartorya and in some other genera within the order Eurotiales that also include Byssochlamys and Talaromyces. The presence of oligosaccharides was observed in species that had a relatively high growth temperature. TOS glasses have a higher glass transition temperature (Tg ) than trehalose, and they form a stable glass with crystallizing molecules, such as mannitol. Our data indicate that TOS are important for prolonged stabilization of cells against stress. The possible unique role of these solutes in protection against dry heat conditions is discussed.
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Functionality and prevalence of trehalose-based oligosaccharides as novel compatible solutes in ascospores of Neosartorya fischeri (Aspergillus fischeri) and other fungi
Environmental microbiology, 2014Co-Authors: Timon T Wyatt, Elena A. Golovina, Edward A. Palumbo, Alex E.e. Verkennis, Nicole L Snyder, Folkert A. Hoekstra, Eric J Kuenstner, C.m. Visagie, M. Richard Van Leeuwen, John E. HallsworthAbstract:Ascospores of Neosartorya, Byssochlamys and Talaromyces can be regarded as the most stress-resistant eukaryotic cells. They can survive exposure at temperatures as high as 85°C for 100 min or more. Neosartorya fischeri ascospores are more viscous and more resistant to the combined stress of heat and desiccation than the ascospores of Talaromyces macrosporus which contain predominantly trehalose. These ascospores contain trehalose-based oligosaccharides (TOS) that are novel compatible solutes, which are accumulated to high levels. These compounds are also found in other members of the genus Neosartorya and in some other genera within the order Eurotiales that also include Byssochlamys and Talaromyces. The presence of oligosaccharides was observed in species that had a relatively high growth temperature. TOS glasses have a higher glass transition temperature (Tg ) than trehalose, and they form a stable glass with crystallizing molecules, such as mannitol. Our data indicate that TOS are important for prolonged stabilization of cells against stress. The possible unique role of these solutes in protection against dry heat conditions is discussed.