The Experts below are selected from a list of 105 Experts worldwide ranked by ideXlab platform

Andrea Dawson - One of the best experts on this subject based on the ideXlab platform.

  • An effect of Nerve growth factor on neurite outgrowth (Nerve Cell Culture/ciliary ganglion neurons/conditioned medium/insi
    2016
    Co-Authors: Frank H. Collins, Andrea Dawson
    Abstract:

    Addition of Nerve growth factor (NGF) to dis- sociated parasympathetic ciliary ganglion neurons resulted, within 60 min of its addition, in a 2-fold increase in average neurite length and an accompanying enlargement and spreading of neu- ronal growth cones. These effects occurred over a concentration range of NGF of 0.1-10 ng/ml and were blocked by affinity-pu- rified antibody to NGF. Epidermal growth factor, fibroblast growth factor, and angiotensin did not have these effects, al- though insulin at high concentrations was able to induce a re- sponse similar to that of NGF. Dissociated sympathetic chain neurons also responded to NGF with increased neurite lengths, and, in addition, NGF considerably extended the survival time of these neurons in Culture. However, the effect of NGF on cil- iary ganglion neurons was limited to neurite outgrowth, and NGF did not promote the survival of these parasympathetic neurons. Nerve growth factor (NGF) promotes neurite outgrowth, neu- ronal survival, and other physiological changes in peripheral sympathetic and sensory neurons and a number of neuron-like clonal Cell lines (1-6). However, there has been no evidence of a response of peripheral parasympathetic neurons to NGF. We present evidence for such a response in the present report. This evidence is based on an assay system we have recently devel- oped (7) in which dissociated parasympathetic ciliary ganglion neurons are Cultured in the presence of a substratum-condi- tioning factor from heart Cell conditioned medium (8-10). In this system the rate of neurite outgrowth is sensitive to the ad- dition of specific agents present in conditioned medium (7) and rat hippocampal extracts (11), and, as shown here, it is sensitive to NGF.

  • an effect of Nerve growth factor on neurite outgrowth Nerve Cell Culture ciliary ganglion neurons conditioned medium insi
    2016
    Co-Authors: Frank H. Collins, Andrea Dawson
    Abstract:

    Addition of Nerve growth factor (NGF) to dis- sociated parasympathetic ciliary ganglion neurons resulted, within 60 min of its addition, in a 2-fold increase in average neurite length and an accompanying enlargement and spreading of neu- ronal growth cones. These effects occurred over a concentration range of NGF of 0.1-10 ng/ml and were blocked by affinity-pu- rified antibody to NGF. Epidermal growth factor, fibroblast growth factor, and angiotensin did not have these effects, al- though insulin at high concentrations was able to induce a re- sponse similar to that of NGF. Dissociated sympathetic chain neurons also responded to NGF with increased neurite lengths, and, in addition, NGF considerably extended the survival time of these neurons in Culture. However, the effect of NGF on cil- iary ganglion neurons was limited to neurite outgrowth, and NGF did not promote the survival of these parasympathetic neurons. Nerve growth factor (NGF) promotes neurite outgrowth, neu- ronal survival, and other physiological changes in peripheral sympathetic and sensory neurons and a number of neuron-like clonal Cell lines (1-6). However, there has been no evidence of a response of peripheral parasympathetic neurons to NGF. We present evidence for such a response in the present report. This evidence is based on an assay system we have recently devel- oped (7) in which dissociated parasympathetic ciliary ganglion neurons are Cultured in the presence of a substratum-condi- tioning factor from heart Cell conditioned medium (8-10). In this system the rate of neurite outgrowth is sensitive to the ad- dition of specific agents present in conditioned medium (7) and rat hippocampal extracts (11), and, as shown here, it is sensitive to NGF.

M. Wagner - One of the best experts on this subject based on the ideXlab platform.

  • PCBs have a predominantly neurotoxic effect on dissociated Cultures of the nervous system.
    Neurotoxicology, 1994
    Co-Authors: F. Boegner, D. Franke, H. Altenkirch, G. Stoltenburg, M. Wagner
    Abstract:

    Although their manufacture and use have been restricted or banned in Europe and the United States since the 1970s, polychlorinated biphenyls (PCBs) are still an ubiquitous environmental contaminant whose low-term effects are as yet not completely clear. Clinical case studies of patients with occupational exposure report cognitive impairment and peripheral neuropathy. In our defined Nerve Cell Culture models in which we use pure neurons, pure glia and mixed Cultures prepared from dorsal root ganglia of chick embryos we observed a neurotoxic effect after the application of a PCB compound (Clophen)

Frank H. Collins - One of the best experts on this subject based on the ideXlab platform.

  • An effect of Nerve growth factor on neurite outgrowth (Nerve Cell Culture/ciliary ganglion neurons/conditioned medium/insi
    2016
    Co-Authors: Frank H. Collins, Andrea Dawson
    Abstract:

    Addition of Nerve growth factor (NGF) to dis- sociated parasympathetic ciliary ganglion neurons resulted, within 60 min of its addition, in a 2-fold increase in average neurite length and an accompanying enlargement and spreading of neu- ronal growth cones. These effects occurred over a concentration range of NGF of 0.1-10 ng/ml and were blocked by affinity-pu- rified antibody to NGF. Epidermal growth factor, fibroblast growth factor, and angiotensin did not have these effects, al- though insulin at high concentrations was able to induce a re- sponse similar to that of NGF. Dissociated sympathetic chain neurons also responded to NGF with increased neurite lengths, and, in addition, NGF considerably extended the survival time of these neurons in Culture. However, the effect of NGF on cil- iary ganglion neurons was limited to neurite outgrowth, and NGF did not promote the survival of these parasympathetic neurons. Nerve growth factor (NGF) promotes neurite outgrowth, neu- ronal survival, and other physiological changes in peripheral sympathetic and sensory neurons and a number of neuron-like clonal Cell lines (1-6). However, there has been no evidence of a response of peripheral parasympathetic neurons to NGF. We present evidence for such a response in the present report. This evidence is based on an assay system we have recently devel- oped (7) in which dissociated parasympathetic ciliary ganglion neurons are Cultured in the presence of a substratum-condi- tioning factor from heart Cell conditioned medium (8-10). In this system the rate of neurite outgrowth is sensitive to the ad- dition of specific agents present in conditioned medium (7) and rat hippocampal extracts (11), and, as shown here, it is sensitive to NGF.

  • an effect of Nerve growth factor on neurite outgrowth Nerve Cell Culture ciliary ganglion neurons conditioned medium insi
    2016
    Co-Authors: Frank H. Collins, Andrea Dawson
    Abstract:

    Addition of Nerve growth factor (NGF) to dis- sociated parasympathetic ciliary ganglion neurons resulted, within 60 min of its addition, in a 2-fold increase in average neurite length and an accompanying enlargement and spreading of neu- ronal growth cones. These effects occurred over a concentration range of NGF of 0.1-10 ng/ml and were blocked by affinity-pu- rified antibody to NGF. Epidermal growth factor, fibroblast growth factor, and angiotensin did not have these effects, al- though insulin at high concentrations was able to induce a re- sponse similar to that of NGF. Dissociated sympathetic chain neurons also responded to NGF with increased neurite lengths, and, in addition, NGF considerably extended the survival time of these neurons in Culture. However, the effect of NGF on cil- iary ganglion neurons was limited to neurite outgrowth, and NGF did not promote the survival of these parasympathetic neurons. Nerve growth factor (NGF) promotes neurite outgrowth, neu- ronal survival, and other physiological changes in peripheral sympathetic and sensory neurons and a number of neuron-like clonal Cell lines (1-6). However, there has been no evidence of a response of peripheral parasympathetic neurons to NGF. We present evidence for such a response in the present report. This evidence is based on an assay system we have recently devel- oped (7) in which dissociated parasympathetic ciliary ganglion neurons are Cultured in the presence of a substratum-condi- tioning factor from heart Cell conditioned medium (8-10). In this system the rate of neurite outgrowth is sensitive to the ad- dition of specific agents present in conditioned medium (7) and rat hippocampal extracts (11), and, as shown here, it is sensitive to NGF.

F. Boegner - One of the best experts on this subject based on the ideXlab platform.

  • PCBs have a predominantly neurotoxic effect on dissociated Cultures of the nervous system.
    Neurotoxicology, 1994
    Co-Authors: F. Boegner, D. Franke, H. Altenkirch, G. Stoltenburg, M. Wagner
    Abstract:

    Although their manufacture and use have been restricted or banned in Europe and the United States since the 1970s, polychlorinated biphenyls (PCBs) are still an ubiquitous environmental contaminant whose low-term effects are as yet not completely clear. Clinical case studies of patients with occupational exposure report cognitive impairment and peripheral neuropathy. In our defined Nerve Cell Culture models in which we use pure neurons, pure glia and mixed Cultures prepared from dorsal root ganglia of chick embryos we observed a neurotoxic effect after the application of a PCB compound (Clophen)

Mohammad Ali Shokrgozar - One of the best experts on this subject based on the ideXlab platform.

  • The influence of surface nanoroughness of electrospun PLGA nanofibrous scaffold on Nerve Cell adhesion and proliferation
    Journal of Materials Science: Materials in Medicine, 2013
    Co-Authors: Fatemeh Zamani, Masoud Latifi, Mohammad Amani-tehran, Mohammad Ali Shokrgozar
    Abstract:

    Electrospun nanofibrous scaffolds in neural tissue engineering provide an alternative approach for neural regeneration. Since the topography of a surface affects the microscopic behaviour of material; the creation of nanoscale surface features, which mimic the natural roughness of live tissue, on polymer surfaces can promote an appropriate Cell growth and proliferation. In this research, a unique PLGA nanofibrous structure was fabricated without any post-electrospinning treatment. Scaffolds were prepared in two general groups: cylindrical and ribbon-shaped electrospun fibres, with smooth and rough (porous and grooved) surfaces. The experiments about Nerve Cell Culture have demonstrated that the nanoroughness of PLGA electrospun scaffolds can increase the Cell growing rate to 50 % in comparison with smooth and conventional electrospun scaffolds. SEM and AFM images and MTT assay results have shown that the roughened cylindrical scaffolds enhance the Nerve growth and proliferation compared to smooth and ribbon-shaped nanofibrous scaffolds. A linear interaction has been found between Cell proliferation and surface features. This helps to approximate MTT assay results by roughness parameters.