The Experts below are selected from a list of 1575 Experts worldwide ranked by ideXlab platform
Seeram Ramakrishna - One of the best experts on this subject based on the ideXlab platform.
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Fabrication of Nerve Growth Factor Encapsulated Aligned Poly(ε-Caprolactone) Nanofibers and Their Assessment as a Potential Neural Tissue Engineering Scaffold
Polymers, 2016Co-Authors: Jue Hu, Molamma P. Prabhakaran, Lingling Tian, Xin Ding, Seeram RamakrishnaAbstract:Peripheral nerve injury is a serious clinical problem to be solved. There has been no breakthrough so far and Neural Tissue Engineering offers a promising approach to promote the regeneration of peripheral Neural injuries. In this study, emulsion electrospinning technique was introduced as a flexible and promising technique for the fabrication of random (R) and aligned (A) Poly(e-caprolactone) (PCL)-Nerve Growth Factor (NGF)B MTS] assay revealed that (R/A)-PCL-NGF and (R/A)-PCL-NGF&BSA scaffolds favored cell growth and showed no cytotoxicity to PC12 cells. Laser scanning confocal microscope images exhibited that the A-PCL-NGF&BSA scaffold increased the length of neurites and directed neurites extension along the fiber axis, indicating that the A-PCL-NGF&BSA scaffold has a potential for guiding nerve Tissue growth and promoting nerve regeneration.
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fabrication of nerve growth factor encapsulated aligned poly e caprolactone nanofibers and their assessment as a potential Neural Tissue Engineering scaffold
Polymers, 2016Co-Authors: Jue Hu, Molamma P. Prabhakaran, Lingling Tian, Xin Ding, Seeram RamakrishnaAbstract:Peripheral nerve injury is a serious clinical problem to be solved. There has been no breakthrough so far and Neural Tissue Engineering offers a promising approach to promote the regeneration of peripheral Neural injuries. In this study, emulsion electrospinning technique was introduced as a flexible and promising technique for the fabrication of random (R) and aligned (A) Poly(e-caprolactone) (PCL)-Nerve Growth Factor (NGF)B MTS] assay revealed that (R/A)-PCL-NGF and (R/A)-PCL-NGF&BSA scaffolds favored cell growth and showed no cytotoxicity to PC12 cells. Laser scanning confocal microscope images exhibited that the A-PCL-NGF&BSA scaffold increased the length of neurites and directed neurites extension along the fiber axis, indicating that the A-PCL-NGF&BSA scaffold has a potential for guiding nerve Tissue growth and promoting nerve regeneration.
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aligned and random nanofibrous substrate for the in vitro culture of schwann cells for Neural Tissue Engineering
Acta Biomaterialia, 2009Co-Authors: Deepika Gupta, Aw Tar Choon, Molamma P. Prabhakaran, Jayarama Reddy Venugopal, Seeram RamakrishnaAbstract:Abstract The current challenge in peripheral nerve Tissue Engineering is to produce an implantable scaffold capable of bridging long nerve gaps that will produce results similar to autograft without requiring the harvest of autologous donor Tissue. Aligned and random polycaprolactone/gelatin (PCL/gelatin) nanofibrous scaffolds were fabricated for the in vitro culture of Schwann cells that assist in directing the growth of regenerating axons in nerve Tissue Engineering. The average fiber diameter attained by electrospinning of polymer blend (PCL/gelatin) ranged from 232 ± 194 to 160 ± 86 nm with high porosity (90%). Blending PCL with gelatin resulted in increased hydrophilicity of nanofibrous scaffolds and yielded better mechanical properties, approaching those of PCL nanofibers. The biocompatibility of fabricated nanofibers was assessed for culturing and proliferation of Schwann cells by MTS assay. The results of the MTS assay and scanning electron microscopy confirmed that aligned and random PCL/gelatin nanofibrous scaffolds are suitable substrates for Schwann cell growth as compared to PCL nanofibrous scaffolds for Neural Tissue Engineering.
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Aligned and random nanofibrous substrate for the in vitro culture of Schwann cells for Neural Tissue Engineering
Acta Biomaterialia, 2009Co-Authors: Deepika Gupta, Sharon Low, Aw Tar Choon, V R Giri Dev, Molamma P. Prabhakaran, Jayarama Reddy Venugopal, Seeram RamakrishnaAbstract:The current challenge in peripheral nerve Tissue Engineering is to produce an implantable scaffold capable of bridging long nerve gaps that will produce results similar to autograft without requiring the harvest of autologous donor Tissue. Aligned and random polycaprolactone/gelatin (PCL/gelatin) nanofibrous scaffolds were fabricated for the in vitro culture of Schwann cells that assist in directing the growth of regenerating axons in nerve Tissue Engineering. The average fiber diameter attained by electrospinning of polymer blend (PCL/gelatin) ranged from 232 ± 194 to 160 ± 86 nm with high porosity (90%). Blending PCL with gelatin resulted in increased hydrophilicity of nanofibrous scaffolds and yielded better mechanical properties, approaching those of PCL nanofibers. The biocompatibility of fabricated nanofibers was assessed for culturing and proliferation of Schwann cells by MTS assay. The results of the MTS assay and scanning electron microscopy confirmed that aligned and random PCL/gelatin nanofibrous scaffolds are suitable substrates for Schwann cell growth as compared to PCL nanofibrous scaffolds for Neural Tissue Engineering. © 2009 Acta Materialia Inc.
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electrospinning of nano micro scale poly l lactic acid aligned fibers and their potential in Neural Tissue Engineering
Biomaterials, 2005Co-Authors: Fang Yang, Ramaswamy Murugan, Shu Wang, Seeram RamakrishnaAbstract:Abstract Efficacy of aligned poly( l -lactic acid) (PLLA) nano/micro fibrous scaffolds for Neural Tissue Engineering is described and their performance with random PLLA scaffolds is compared as well in this study. Perfectly aligned PLLA fibrous scaffolds were fabricated by an electrospinning technique under optimum condition and the diameter of the electrospun fibers can easily be tailored by adjusting the concentration of polymer solution. As the structure of PLLA scaffold was intended for Neural Tissue Engineering, its suitability was evaluated in vitro using Neural stem cells (NSCs) as a model cell line. Cell morphology, differentiation and neurite outgrowth were studied by various microscopic techniques. The results show that the direction of NSC elongation and its neurite outgrowth is parallel to the direction of PLLA fibers for aligned scaffolds. No significant changes were observed on the cell orientation with respect to the fiber diameters. However, the rate of NSC differentiation was higher for PLLA nanofibers than that of micro fibers and it was independent of the fiber alignment. Based on the experimental results, the aligned nanofibrous PLLA scaffold could be used as a potential cell carrier in Neural Tissue Engineering.
Stephanie M. Willerth - One of the best experts on this subject based on the ideXlab platform.
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Neural Tissue Engineering using embryonic and induced pluripotent stem cells
Stem Cell Research & Therapy, 2011Co-Authors: Stephanie M. WillerthAbstract:With the recent start of the first clinical trial evaluating a human embryonic stem cell-derived therapy for the treatment of acute spinal cord injury, it is important to review the current literature examining the use of embryonic stem cells for Neural Tissue Engineering applications with a focus on diseases and disorders that affect the central nervous system. Embryonic stem cells exhibit pluripotency and thus can differentiate into any cell type found in the body, including those found in the nervous system. A range of studies have investigated how to direct the differentiation of embryonic cells into specific Neural phenotypes using a variety of cues to achieve the goal of replacing diseased or damaged Neural Tissue. Additionally, the recent development of induced pluripotent stem cells provides an intriguing alternative to the use of human embryonic stem cell lines for these applications. This review will discuss relevant studies that have used embryonic stem cells to replicate the Tissue found in the central nervous system as well as evaluate the potential of induced pluripotent stem cells for the aforementioned applications.
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conductive core sheath nanofibers and their potential application in Neural Tissue Engineering
Advanced Functional Materials, 2009Co-Authors: Matthew R. Macewan, Daniel W. Moran, Stephanie M. Willerth, Xiaoran Li, Shelly E SakiyamaelbertAbstract:We have prepared conductive core-sheath nanofibers via a combination of electrospinning and aqueous polymerization. Specifically, nanofibers electrospun from poly(e-caprolactone) (PCL) and poly(L-lactide) (PLA) were employed as templates to generate uniform sheaths of polypyrrole (PPy) via in situ polymerization. These conductive core-sheath nanofibers offer a unique system for studying the synergistic effect of different cues on neurite outgrowth in vitro. We found that explanted dorsal root ganglia (DRG) adhered well to the conductive core-sheath nanofibers and generated neurites across the surface when there was a nerve growth factor in the medium. Furthermore, the neurites could be oriented along one direction and enhanced by 82% in terms of maximum length when uniaxially aligned conductive core-sheath nanofibers are compared with their random counterparts. Electrical stimulation, when applied through the mats of conductive core-sheath nanofibers, was found to further increase the maximum length of neurite for random and aligned samples by 83% and 47%, respectively, relative to the controls without electrical stimulation. Combined together, these results suggest the potential use of the conductive core-sheath nanofibers as scaffolds in applications such as Neural Tissue Engineering.
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Conductive Core-Sheath Nanofibers and Their Potential Application in Neural Tissue Engineering
Advanced Functional Materials, 2009Co-Authors: Jingwei Xie, Daniel W. Moran, Matthew R. Macewan, Shelly E Sakiyama-elbert, Stephanie M. Willerth, Xiaoran Li, Younan XiaAbstract:We have prepared conductive core-sheath nanofibers via a combination of electrospinning and aqueous polymerization. Specifically, nanofibers electrospun from poly(ε-caprolactone) (PCL) and poly((L)-lactide) (PLA) were employed as templates to generate uniform sheaths of polypyrrole (PPy) via in situ polymerization. These conductive core-sheath nanofibers offer a unique system for studying the synergistic effect of different cues on neurite outgrowth in vitro. We found that explanted dorsal root ganglia (DRG) adhered well to the conductive core-sheath nanofibers and generated neurites across the surface when there was a nerve growth factor in the medium. Furthermore, the neurites could be oriented along one direction and enhanced by 82% in terms of maximum length when uniaxially aligned conductive core-sheath nanofibers are compared with their random counterparts. Electrical stimulation, when applied through the mats of conductive core-sheath nanofibers, was found to further increase the maximum length of neurite for random and aligned samples by 83% and 47%, respectively, relative to the controls without electrical stimulation. Combined together, these results suggest the potential use of the conductive core-sheath nanofibers as scaffolds in applications such as Neural Tissue Engineering.
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approaches to Neural Tissue Engineering using scaffolds for drug delivery
Advanced Drug Delivery Reviews, 2007Co-Authors: Stephanie M. Willerth, Shelly E SakiyamaelbertAbstract:This review seeks to give an overview of the current approaches to drug delivery from scaffolds for Neural Tissue Engineering applications. The challenges presented by attempting to replicate the three types of nervous Tissue (brain, spinal cord, and peripheral nerve) are summarized. Potential scaffold materials (both synthetic and natural) and target drugs are discussed with the benefits and drawbacks given. Finally, common methods of drug delivery, including degradable/diffusion-based delivery systems, affinity-based delivery systems, immobilized drug delivery systems, and electrically controlled drug delivery systems, are examined and critiqued. Based on the current body of work, suggestions for future directions of research in the field of Neural Tissue Engineering are presented.
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Approaches to Neural Tissue Engineering using scaffolds for drug delivery
Advanced Drug Delivery Reviews, 2007Co-Authors: Stephanie M. Willerth, Shelly E Sakiyama-elbertAbstract:This review seeks to give an overview of the current approaches to drug delivery from scaffolds for Neural Tissue Engineering applications. The challenges presented by attempting to replicate the three types of nervous Tissue (brain, spinal cord, and peripheral nerve) are summarized. Potential scaffold materials (both synthetic and natural) and target drugs are discussed with the benefits and drawbacks given. Finally, common methods of drug delivery, including degradable/diffusion-based delivery systems, affinity-based delivery systems, immobilized drug delivery systems, and electrically controlled drug delivery systems, are examined and critiqued. Based on the current body of work, suggestions for future directions of research in the field of Neural Tissue Engineering are presented. © 2007 Elsevier B.V. All rights reserved.
Shelly E Sakiyamaelbert - One of the best experts on this subject based on the ideXlab platform.
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conductive core sheath nanofibers and their potential application in Neural Tissue Engineering
Advanced Functional Materials, 2009Co-Authors: Matthew R. Macewan, Daniel W. Moran, Stephanie M. Willerth, Xiaoran Li, Shelly E SakiyamaelbertAbstract:We have prepared conductive core-sheath nanofibers via a combination of electrospinning and aqueous polymerization. Specifically, nanofibers electrospun from poly(e-caprolactone) (PCL) and poly(L-lactide) (PLA) were employed as templates to generate uniform sheaths of polypyrrole (PPy) via in situ polymerization. These conductive core-sheath nanofibers offer a unique system for studying the synergistic effect of different cues on neurite outgrowth in vitro. We found that explanted dorsal root ganglia (DRG) adhered well to the conductive core-sheath nanofibers and generated neurites across the surface when there was a nerve growth factor in the medium. Furthermore, the neurites could be oriented along one direction and enhanced by 82% in terms of maximum length when uniaxially aligned conductive core-sheath nanofibers are compared with their random counterparts. Electrical stimulation, when applied through the mats of conductive core-sheath nanofibers, was found to further increase the maximum length of neurite for random and aligned samples by 83% and 47%, respectively, relative to the controls without electrical stimulation. Combined together, these results suggest the potential use of the conductive core-sheath nanofibers as scaffolds in applications such as Neural Tissue Engineering.
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approaches to Neural Tissue Engineering using scaffolds for drug delivery
Advanced Drug Delivery Reviews, 2007Co-Authors: Stephanie M. Willerth, Shelly E SakiyamaelbertAbstract:This review seeks to give an overview of the current approaches to drug delivery from scaffolds for Neural Tissue Engineering applications. The challenges presented by attempting to replicate the three types of nervous Tissue (brain, spinal cord, and peripheral nerve) are summarized. Potential scaffold materials (both synthetic and natural) and target drugs are discussed with the benefits and drawbacks given. Finally, common methods of drug delivery, including degradable/diffusion-based delivery systems, affinity-based delivery systems, immobilized drug delivery systems, and electrically controlled drug delivery systems, are examined and critiqued. Based on the current body of work, suggestions for future directions of research in the field of Neural Tissue Engineering are presented.
Shahriar Hojjati-emami - One of the best experts on this subject based on the ideXlab platform.
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Cellulose acetate/poly lactic acid coaxial wet-electrospun scaffold containing citalopram-loaded gelatin nanocarriers for Neural Tissue Engineering applications
International Journal of Biological Macromolecules, 2017Co-Authors: Mahdi Naseri-nosar, Majid Salehi, Shahriar Hojjati-emamiAbstract:The current study aimed to develop a biodegradable three-dimensional drug-loaded scaffold with the core–shell structured fibrils using coaxial wet-electrospinning for Neural Tissue Engineering application. Poly lactic acid was wet-electrospun as the core, whereas cellulose acetate was fabricated into the fibril's shell. The scaffold then was coated with the citalopram-loaded gelatin nanocarriers (CGNs) produced by nanoprecipitation method. Scanning electron microscope observation revealed that the fibrils formed a nonwoven structure with the average diameter of ∼950 nm. The particle size measurement by a dynamic light scattering device showed an average diameter of ∼200 nm. The porosity measurement via the liquid displacement method showed that the scaffold could not meet the accepted ideal porosity percentage of above 80%, and the measured porosity percentage was ∼60%. The contact angle measurement displayed that the CGN coating made the scaffold highly hydrophilic with a zero degree contact angle. In vitro degradation study in the phosphate buffered saline revealed that the weight of the uncoated scaffold remained relatively constant. However, the CGNs-coated scaffold showed ∼45% weight-loss percentage after 40 days. Cytocompatibility evaluation using rat Schwann cells demonstrated that the CGNs-coated scaffold possessed higher cell viability than the uncoated scaffold. Finally, the scaffold was developed into a nerve guidance conduit and surgically implanted in the sciatic nerve defect in Wistar rats. The results of the sciatic functional index, hot plate latency and weight-loss percentage of the wet gastrocnemius muscle, demonstrated that the citalopram-containing scaffold could ameliorate the functional recovery of the sciatic nerve-injured animals which makes it a potential candidate for the Neural Tissue Engineering applications.
Shahriar Hojjatiemami - One of the best experts on this subject based on the ideXlab platform.
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cellulose acetate poly lactic acid coaxial wet electrospun scaffold containing citalopram loaded gelatin nanocarriers for Neural Tissue Engineering applications
International Journal of Biological Macromolecules, 2017Co-Authors: Mahdi Naserinosar, Majid Salehi, Shahriar HojjatiemamiAbstract:Abstract The current study aimed to develop a biodegradable three-dimensional drug-loaded scaffold with the core–shell structured fibrils using coaxial wet-electrospinning for Neural Tissue Engineering application. Poly lactic acid was wet-electrospun as the core, whereas cellulose acetate was fabricated into the fibril’s shell. The scaffold then was coated with the citalopram-loaded gelatin nanocarriers (CGNs) produced by nanoprecipitation method. Scanning electron microscope observation revealed that the fibrils formed a nonwoven structure with the average diameter of ∼950 nm. The particle size measurement by a dynamic light scattering device showed an average diameter of ∼200 nm. The porosity measurement via the liquid displacement method showed that the scaffold could not meet the accepted ideal porosity percentage of above 80%, and the measured porosity percentage was ∼60%. The contact angle measurement displayed that the CGN coating made the scaffold highly hydrophilic with a zero degree contact angle. In vitro degradation study in the phosphate buffered saline revealed that the weight of the uncoated scaffold remained relatively constant. However, the CGNs-coated scaffold showed ∼45% weight-loss percentage after 40 days. Cytocompatibility evaluation using rat Schwann cells demonstrated that the CGNs-coated scaffold possessed higher cell viability than the uncoated scaffold. Finally, the scaffold was developed into a nerve guidance conduit and surgically implanted in the sciatic nerve defect in Wistar rats. The results of the sciatic functional index, hot plate latency and weight-loss percentage of the wet gastrocnemius muscle, demonstrated that the citalopram-containing scaffold could ameliorate the functional recovery of the sciatic nerve-injured animals which makes it a potential candidate for the Neural Tissue Engineering applications.