The Experts below are selected from a list of 180 Experts worldwide ranked by ideXlab platform
Sérgio Akira Uyemura - One of the best experts on this subject based on the ideXlab platform.
-
Heterologous expression of mitochondrial Nicotinamide Adenine Dinucleotide transporter (Ndt1) from Aspergillus fumigatus rescues impaired growth in Δndt1Δndt2 Saccharomyces cerevisiae strain
Journal of Bioenergetics and Biomembranes, 2017Co-Authors: Laís De Lourdes De Lima Balico, Emerson Souza Santos, Silveli Suzuki-hatano, Lucas Oliveira Sousa, Ana Elisa Caleiro Seixas Azzolini, Yara Maria Lucisano-valim, Taisa Magnani Dinamarco, Vinicius Kannen, Sérgio Akira UyemuraAbstract:Our understanding of Nicotinamide Adenine Dinucleotide mitochondrial transporter 1 (Ndt1A) in Aspergillus fumigatus remains poor. Thus, we investigated whether Ndt1A could alter fungi survival. To this end, we engineered the expression of an Ndt1A-encoding region in a Δndt1Δndt2 yeast strain. The resulting cloned Ndt1A protein promoted the mitochondrial uptake of Nicotinamide Adenine Dinucleotide (NAD^+), generating a large mitochondrial membrane potential. The NAD^+ carrier utilized the electrochemical proton gradient to drive NAD^+ entrance into mitochondria when the mitochondrial membrane potential was sustained by succinate. Its uptake has no impact on oxidative stress, and Ndt1A expression improved growth and survival of the Δndt1Δndt2 Saccharomyces cerevisiae strain.
Leonard Guarente - One of the best experts on this subject based on the ideXlab platform.
-
Nicotinamide Adenine Dinucleotide a metabolic regulator of transcription longevity and disease
Current Opinion in Cell Biology, 2003Co-Authors: Leonard GuarenteAbstract:Nicotinamide Adenine Dinucleotide (NAD) is a ubiquitous biological molecule that participates in many metabolic reactions. Recent studies show that NAD also plays important roles in transcriptional regulation, longevity, calorie-restriction-mediated life-span extension and age-associated diseases. It has been shown that NAD affects longevity and transcriptional silencing through the regulation of the Sir2p family, which are NAD-dependent deacetylases. Many human diseases are associated with changes in NAD level and/or the NAD : NADH ratio, raising the possibility that the Sir2p family might play a role in these diseases.
Joseph A Baur - One of the best experts on this subject based on the ideXlab platform.
-
Nicotinamide Adenine Dinucleotide biosynthesis promotes liver regeneration
Hepatology, 2017Co-Authors: Sarmistha Mukherjee, Karthikeyani Chellappa, Andrea Moffitt, Joan Ndungu, Ryan Dellinger, James G Davis, Beamon Agarwal, Joseph A BaurAbstract:The regenerative capacity of the liver is essential for recovery from surgical resection or injuries induced by trauma or toxins. During liver regeneration, the concentration of Nicotinamide Adenine Dinucleotide (NAD) falls, at least in part due to metabolic competition for precursors. To test whether NAD availability restricts the rate of liver regeneration, we supplied Nicotinamide riboside (NR), an NAD precursor, in the drinking water of mice subjected to partial hepatectomy. NR increased DNA synthesis, mitotic index, and mass restoration in the regenerating livers. Intriguingly, NR also ameliorated the steatosis that normally accompanies liver regeneration. To distinguish the role of hepatocyte NAD levels from any systemic effects of NR, we generated mice overexpressing Nicotinamide phosphoribosyltransferase, a rate-limiting enzyme for NAD synthesis, specifically in the liver. Nicotinamide phosphoribosyltransferase overexpressing mice were mildly hyperglycemic at baseline and, similar to mice treated with NR, exhibited enhanced liver regeneration and reduced steatosis following partial hepatectomy. Conversely, mice lacking Nicotinamide phosphoribosyltransferase in hepatocytes exhibited impaired regenerative capacity that was completely rescued by administering NR. Conclusion: NAD availability is limiting during liver regeneration, and supplementation with precursors such as NR may be therapeutic in settings of acute liver injury. (Hepatology 2017;65:616-630).
Hsingwen Wang - One of the best experts on this subject based on the ideXlab platform.
-
reduced Nicotinamide Adenine Dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies
Journal of Biomedical Optics, 2008Co-Authors: Hanwen Guo, Chientsun Chen, Yauhuei Wei, Oscar K Lee, Vladimir Gukassyan, Fujen Kao, Hsingwen WangAbstract:The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced Nicotinamide Adenine Dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.
Laís De Lourdes De Lima Balico - One of the best experts on this subject based on the ideXlab platform.
-
Heterologous expression of mitochondrial Nicotinamide Adenine Dinucleotide transporter (Ndt1) from Aspergillus fumigatus rescues impaired growth in Δndt1Δndt2 Saccharomyces cerevisiae strain
Journal of Bioenergetics and Biomembranes, 2017Co-Authors: Laís De Lourdes De Lima Balico, Emerson Souza Santos, Silveli Suzuki-hatano, Lucas Oliveira Sousa, Ana Elisa Caleiro Seixas Azzolini, Yara Maria Lucisano-valim, Taisa Magnani Dinamarco, Vinicius Kannen, Sérgio Akira UyemuraAbstract:Our understanding of Nicotinamide Adenine Dinucleotide mitochondrial transporter 1 (Ndt1A) in Aspergillus fumigatus remains poor. Thus, we investigated whether Ndt1A could alter fungi survival. To this end, we engineered the expression of an Ndt1A-encoding region in a Δndt1Δndt2 yeast strain. The resulting cloned Ndt1A protein promoted the mitochondrial uptake of Nicotinamide Adenine Dinucleotide (NAD^+), generating a large mitochondrial membrane potential. The NAD^+ carrier utilized the electrochemical proton gradient to drive NAD^+ entrance into mitochondria when the mitochondrial membrane potential was sustained by succinate. Its uptake has no impact on oxidative stress, and Ndt1A expression improved growth and survival of the Δndt1Δndt2 Saccharomyces cerevisiae strain.