The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
Xiao Fei Huang - One of the best experts on this subject based on the ideXlab platform.
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effect of trace elements and optimization of their composition for the nitrification of a heterotrophic Nitrifying Bacterium acinetobacter harbinensis hitli7 t at low temperature
Annals of Microbiology, 2017Co-Authors: Zejia Zheng, Wei Guang Li, Xiao Fei HuangAbstract:The effects of trace elements on ammonium degradation performance and extracellular polymeric substances (EPS) secretion of Acinetobacter harbinensis HITLi7T at low temperature were investigated. Response surface methodology (RSM) was applied to obtain the optimal composition of trace elements and analyze their correlation. In this study, the results indicated that the ammonium removal performance could be enhanced by the presence of 0.1 mg L−1 Fe, Mn, or B in pure cultivation. When the concentrations of Fe and Mn were 0.2 mg L−1, the ammonium removal rates of the novel strain HITLi7T were 0.49 ± 0.01 mg L−1·h−1 and 0.58 ± 0.01 mg L−1·h−1, respectively, while it was the low concentration of 0.05 mg L−1 B that showed the maximum ammonium removal rate (0.56 ± 0.02 mg L−1·h−1) of strain HITLi7T. The regression model was obtained and the optimal formulation of trace elements was: B 0.064 mg L−1, Fe 0.12 mg L−1, and Mn 0.1 mg L−1. Based on these values, the experimental ammonium removal rate could reach 0.59 mg L−1·h−1, which matched well with the predicted response. The study also found that the addition of trace elements, causing high ammonium removal rates, resulted in a high polysaccharide (PS) ratio in the EPS secreted by Acinetobacter harbinensis HITLi7T. Especially under the optimal conditions, the PS ratio reached the highest value of 49.9%.
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a proteomic analysis of heterotrophic Nitrifying Bacterium acinetobacter sp hitli 7t adaptation to low temperature using two dimensional difference gel electrophoresis approach
International Biodeterioration & Biodegradation, 2016Co-Authors: Wei Guang Li, Duoying Zhang, Xiao Fei Huang, Yang SongAbstract:Abstract Acinetobacter sp. HITLi 7 T showed capacity of heterotrophic nitrification at 2 °C with an optimum growth temperature of 20 °C. The ammonium removal rates were 0.09 and 0.24 mg l −1 h −1 at 2 °C and 20 °C respectively. Cell growth and proliferation affected ammonium removal performance of HITLi 7 T dramatically at low temperature. To investigate the influence of low temperature on protein expression, the response of HITLi 7 T cultivated at 2 °C and 20 °C were characterized and compared by 2-dimensional proteome analyses. A total of 151 proteins were identified to be differentially expressed by the analysis of PDQuest 2D gel analysis software. In comparison to protein expression at 20 °C, 17, 28, and 43 protein spots were found to be up-regulated, down-regulated and only present at 2 °C, respectively. 9 spots, which were overexpressed or induced at low temperature, were successfully identified by peptide mass fingerprinting by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. The significantly up-regulated proteins at 2 °C were related to metabolism. The proteins only present at 2 °C had functions of protein biosynthesis and refolding. These findings indicated that cold adaption of HITLi 7 T relied on regulation of metabolic pathways, synthesis of proteins and protein refolding.
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characteristics of heterotrophic Nitrifying Bacterium strain sfa13 isolated from the songhua river
Annals of Microbiology, 2016Co-Authors: Duoying Zhang, Wei Guang Li, Xiao Fei Huang, Peng WangAbstract:Strain SFA13, with the ability to perform simultaneous heterotrophic nitrification and aerobic denitrification, was isolated from the Songhua River. Investigation of the phenotypic and genotypic characteristics of strain SFA13 revealed that its 16S rRNA gene sequence shares high similarity with that of MicroBacterium esteraromaticum DSM 8609T (99.78 %). gyrB and recA gene sequences showed 92.40 % and 92.57 % similarity to those of M. esteraromaticum DSM 8609T, respectively, showing that strain SFA13 is a member of M. esteraromaticum. However, strain SFA13 showed distinctly different phenotypic characteristics from the type strain DSM 8609T. Strain SFA13 was negative for H2S production, β-fucosidase, hydrolysis of starch and Tween, utilization of salicin, l-alanine, d-fructose, d-galactose, d-glucose and d-trehalose; and positive for alkaline phosphatase, cystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and β-galactosidase. The ammonium removal rate of strain SFA13 was affected by temperature, decreasing obviously as the temperature dropped from 30 °C to 10 °C. Even so, strain SFA13 exhibited a high ammonium removal efficiency at 5 °C. The highest ammonium removal rate was 4.41 mg NH4+ L−1 h−1 when the initial ammonium concentration was 61.63 mg L−1 at 5 °C. Excessive ammonium (more than 62 mg L−1) restricted the ammonium removal rate of strain SFA13. This strain could be used effectively to remove low concentrations of ammonium ion at low temperature.
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Purification and Characterization of a Low-temperature Hydroxylamine Oxidase from Heterotrophic Nitrifier Acinetobacter sp. Y16
Biomedical and Environmental Sciences, 2014Co-Authors: Shu Mei Zhang, Wei Guang Li, Duoying Zhang, Xiao Fei HuangAbstract:Objective To purify a low-temperature hydroxylamine oxidase (HAO) from a heterotrophic Nitrifying Bacterium Acinetobacter sp. Y16 and investigate the enzyme property.
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Characteristics and the Basic Kinetics of Ammonium Removal by Heterotrophic Nitrifying Bacteria at Low Temperature
Advanced Materials Research, 2013Co-Authors: Xiao Fei Huang, Wei Guang Li, Duoying ZhangAbstract:A oligotrophic heterotrophic Nitrifying Bacterium Y21 was isolated from Songhua River at low temperature. The influence of temperature, pH, rotating speed of shaker as well as the carbon sources on the strain Y21 was investigated in this study. The experimental results showed that for the strain Y21, the optimum conditions were found to be temperature of 15 °C, pH of 7.4~8.2, shaking speed of 140 rmp respectively, with sodium acetate as carbon source. The strain Y21 has high activity under the culture condition at low temperature. The basic dynamic equation for the degradation of ammonia-nitrogen was obtained.
Wei Guang Li - One of the best experts on this subject based on the ideXlab platform.
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effect of trace elements and optimization of their composition for the nitrification of a heterotrophic Nitrifying Bacterium acinetobacter harbinensis hitli7 t at low temperature
Annals of Microbiology, 2017Co-Authors: Zejia Zheng, Wei Guang Li, Xiao Fei HuangAbstract:The effects of trace elements on ammonium degradation performance and extracellular polymeric substances (EPS) secretion of Acinetobacter harbinensis HITLi7T at low temperature were investigated. Response surface methodology (RSM) was applied to obtain the optimal composition of trace elements and analyze their correlation. In this study, the results indicated that the ammonium removal performance could be enhanced by the presence of 0.1 mg L−1 Fe, Mn, or B in pure cultivation. When the concentrations of Fe and Mn were 0.2 mg L−1, the ammonium removal rates of the novel strain HITLi7T were 0.49 ± 0.01 mg L−1·h−1 and 0.58 ± 0.01 mg L−1·h−1, respectively, while it was the low concentration of 0.05 mg L−1 B that showed the maximum ammonium removal rate (0.56 ± 0.02 mg L−1·h−1) of strain HITLi7T. The regression model was obtained and the optimal formulation of trace elements was: B 0.064 mg L−1, Fe 0.12 mg L−1, and Mn 0.1 mg L−1. Based on these values, the experimental ammonium removal rate could reach 0.59 mg L−1·h−1, which matched well with the predicted response. The study also found that the addition of trace elements, causing high ammonium removal rates, resulted in a high polysaccharide (PS) ratio in the EPS secreted by Acinetobacter harbinensis HITLi7T. Especially under the optimal conditions, the PS ratio reached the highest value of 49.9%.
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a proteomic analysis of heterotrophic Nitrifying Bacterium acinetobacter sp hitli 7t adaptation to low temperature using two dimensional difference gel electrophoresis approach
International Biodeterioration & Biodegradation, 2016Co-Authors: Wei Guang Li, Duoying Zhang, Xiao Fei Huang, Yang SongAbstract:Abstract Acinetobacter sp. HITLi 7 T showed capacity of heterotrophic nitrification at 2 °C with an optimum growth temperature of 20 °C. The ammonium removal rates were 0.09 and 0.24 mg l −1 h −1 at 2 °C and 20 °C respectively. Cell growth and proliferation affected ammonium removal performance of HITLi 7 T dramatically at low temperature. To investigate the influence of low temperature on protein expression, the response of HITLi 7 T cultivated at 2 °C and 20 °C were characterized and compared by 2-dimensional proteome analyses. A total of 151 proteins were identified to be differentially expressed by the analysis of PDQuest 2D gel analysis software. In comparison to protein expression at 20 °C, 17, 28, and 43 protein spots were found to be up-regulated, down-regulated and only present at 2 °C, respectively. 9 spots, which were overexpressed or induced at low temperature, were successfully identified by peptide mass fingerprinting by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. The significantly up-regulated proteins at 2 °C were related to metabolism. The proteins only present at 2 °C had functions of protein biosynthesis and refolding. These findings indicated that cold adaption of HITLi 7 T relied on regulation of metabolic pathways, synthesis of proteins and protein refolding.
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characteristics of heterotrophic Nitrifying Bacterium strain sfa13 isolated from the songhua river
Annals of Microbiology, 2016Co-Authors: Duoying Zhang, Wei Guang Li, Xiao Fei Huang, Peng WangAbstract:Strain SFA13, with the ability to perform simultaneous heterotrophic nitrification and aerobic denitrification, was isolated from the Songhua River. Investigation of the phenotypic and genotypic characteristics of strain SFA13 revealed that its 16S rRNA gene sequence shares high similarity with that of MicroBacterium esteraromaticum DSM 8609T (99.78 %). gyrB and recA gene sequences showed 92.40 % and 92.57 % similarity to those of M. esteraromaticum DSM 8609T, respectively, showing that strain SFA13 is a member of M. esteraromaticum. However, strain SFA13 showed distinctly different phenotypic characteristics from the type strain DSM 8609T. Strain SFA13 was negative for H2S production, β-fucosidase, hydrolysis of starch and Tween, utilization of salicin, l-alanine, d-fructose, d-galactose, d-glucose and d-trehalose; and positive for alkaline phosphatase, cystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and β-galactosidase. The ammonium removal rate of strain SFA13 was affected by temperature, decreasing obviously as the temperature dropped from 30 °C to 10 °C. Even so, strain SFA13 exhibited a high ammonium removal efficiency at 5 °C. The highest ammonium removal rate was 4.41 mg NH4+ L−1 h−1 when the initial ammonium concentration was 61.63 mg L−1 at 5 °C. Excessive ammonium (more than 62 mg L−1) restricted the ammonium removal rate of strain SFA13. This strain could be used effectively to remove low concentrations of ammonium ion at low temperature.
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Purification and Characterization of a Low-temperature Hydroxylamine Oxidase from Heterotrophic Nitrifier Acinetobacter sp. Y16
Biomedical and Environmental Sciences, 2014Co-Authors: Shu Mei Zhang, Wei Guang Li, Duoying Zhang, Xiao Fei HuangAbstract:Objective To purify a low-temperature hydroxylamine oxidase (HAO) from a heterotrophic Nitrifying Bacterium Acinetobacter sp. Y16 and investigate the enzyme property.
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Characteristics and the Basic Kinetics of Ammonium Removal by Heterotrophic Nitrifying Bacteria at Low Temperature
Advanced Materials Research, 2013Co-Authors: Xiao Fei Huang, Wei Guang Li, Duoying ZhangAbstract:A oligotrophic heterotrophic Nitrifying Bacterium Y21 was isolated from Songhua River at low temperature. The influence of temperature, pH, rotating speed of shaker as well as the carbon sources on the strain Y21 was investigated in this study. The experimental results showed that for the strain Y21, the optimum conditions were found to be temperature of 15 °C, pH of 7.4~8.2, shaking speed of 140 rmp respectively, with sodium acetate as carbon source. The strain Y21 has high activity under the culture condition at low temperature. The basic dynamic equation for the degradation of ammonia-nitrogen was obtained.
Duoying Zhang - One of the best experts on this subject based on the ideXlab platform.
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a proteomic analysis of heterotrophic Nitrifying Bacterium acinetobacter sp hitli 7t adaptation to low temperature using two dimensional difference gel electrophoresis approach
International Biodeterioration & Biodegradation, 2016Co-Authors: Wei Guang Li, Duoying Zhang, Xiao Fei Huang, Yang SongAbstract:Abstract Acinetobacter sp. HITLi 7 T showed capacity of heterotrophic nitrification at 2 °C with an optimum growth temperature of 20 °C. The ammonium removal rates were 0.09 and 0.24 mg l −1 h −1 at 2 °C and 20 °C respectively. Cell growth and proliferation affected ammonium removal performance of HITLi 7 T dramatically at low temperature. To investigate the influence of low temperature on protein expression, the response of HITLi 7 T cultivated at 2 °C and 20 °C were characterized and compared by 2-dimensional proteome analyses. A total of 151 proteins were identified to be differentially expressed by the analysis of PDQuest 2D gel analysis software. In comparison to protein expression at 20 °C, 17, 28, and 43 protein spots were found to be up-regulated, down-regulated and only present at 2 °C, respectively. 9 spots, which were overexpressed or induced at low temperature, were successfully identified by peptide mass fingerprinting by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. The significantly up-regulated proteins at 2 °C were related to metabolism. The proteins only present at 2 °C had functions of protein biosynthesis and refolding. These findings indicated that cold adaption of HITLi 7 T relied on regulation of metabolic pathways, synthesis of proteins and protein refolding.
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characteristics of heterotrophic Nitrifying Bacterium strain sfa13 isolated from the songhua river
Annals of Microbiology, 2016Co-Authors: Duoying Zhang, Wei Guang Li, Xiao Fei Huang, Peng WangAbstract:Strain SFA13, with the ability to perform simultaneous heterotrophic nitrification and aerobic denitrification, was isolated from the Songhua River. Investigation of the phenotypic and genotypic characteristics of strain SFA13 revealed that its 16S rRNA gene sequence shares high similarity with that of MicroBacterium esteraromaticum DSM 8609T (99.78 %). gyrB and recA gene sequences showed 92.40 % and 92.57 % similarity to those of M. esteraromaticum DSM 8609T, respectively, showing that strain SFA13 is a member of M. esteraromaticum. However, strain SFA13 showed distinctly different phenotypic characteristics from the type strain DSM 8609T. Strain SFA13 was negative for H2S production, β-fucosidase, hydrolysis of starch and Tween, utilization of salicin, l-alanine, d-fructose, d-galactose, d-glucose and d-trehalose; and positive for alkaline phosphatase, cystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and β-galactosidase. The ammonium removal rate of strain SFA13 was affected by temperature, decreasing obviously as the temperature dropped from 30 °C to 10 °C. Even so, strain SFA13 exhibited a high ammonium removal efficiency at 5 °C. The highest ammonium removal rate was 4.41 mg NH4+ L−1 h−1 when the initial ammonium concentration was 61.63 mg L−1 at 5 °C. Excessive ammonium (more than 62 mg L−1) restricted the ammonium removal rate of strain SFA13. This strain could be used effectively to remove low concentrations of ammonium ion at low temperature.
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Purification and Characterization of a Low-temperature Hydroxylamine Oxidase from Heterotrophic Nitrifier Acinetobacter sp. Y16
Biomedical and Environmental Sciences, 2014Co-Authors: Shu Mei Zhang, Wei Guang Li, Duoying Zhang, Xiao Fei HuangAbstract:Objective To purify a low-temperature hydroxylamine oxidase (HAO) from a heterotrophic Nitrifying Bacterium Acinetobacter sp. Y16 and investigate the enzyme property.
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Characteristics and the Basic Kinetics of Ammonium Removal by Heterotrophic Nitrifying Bacteria at Low Temperature
Advanced Materials Research, 2013Co-Authors: Xiao Fei Huang, Wei Guang Li, Duoying ZhangAbstract:A oligotrophic heterotrophic Nitrifying Bacterium Y21 was isolated from Songhua River at low temperature. The influence of temperature, pH, rotating speed of shaker as well as the carbon sources on the strain Y21 was investigated in this study. The experimental results showed that for the strain Y21, the optimum conditions were found to be temperature of 15 °C, pH of 7.4~8.2, shaking speed of 140 rmp respectively, with sodium acetate as carbon source. The strain Y21 has high activity under the culture condition at low temperature. The basic dynamic equation for the degradation of ammonia-nitrogen was obtained.
M Wood - One of the best experts on this subject based on the ideXlab platform.
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heterotrophic nitrification in an acid forest soil isolation and characterisation of a Nitrifying Bacterium
Soil Biology & Biochemistry, 2001Co-Authors: E D R Brierley, M WoodAbstract:Abstract Bacteria and fungi were isolated from an acid forest soil in which nitrification occurred via a heterotrophic pathway. Enrichment of soil in a liquid inorganic salts medium, containing β-alanine as the sole source of C and N, led to formation of NO 2 − and NO 3 − . In pure culture, β-alanine was not a suitable substrate for nitrification by any of the isolates, but did support nitrification when one Bacterium ( B D ) was co-cultured with a fungus. This Bacterium was gram-positive and rod-shaped and identified provisionally as an Arthrobacter sp. The Bacterium B D was able to nitrify ammonium acetate and, to a lesser extent peptone, in pure culture. Nitrification of ammonium acetate was greater in sterile soil solution than in defined media of inorganic salts. From a less reduced form of N, α -ketoglutaric oxime supported the highest rates of nitrification, but nitrification of pyruvic and ketobutyric oximes was at levels lower than from reduced N. The organism was able to survive and nitrify at pH 3, and despite its isolation from a very acidic environment, at pH≤10. It is proposed that a metabolic product of β-alanine produced by a non-Nitrifying microorganism provided a suitable substrate for the Nitrifying Bacterium.
Lei Yang - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous Nitrogen and Phosphorus Removal and Kinetics by the Heterotrophic Nitrifying Bacterium Acinetobacter junii NP1
Huan jing ke xue= Huanjing kexue, 2019Co-Authors: Lei Yang, Xu-hui Wang, Ning Chen, Qian XiaoAbstract:Due to the problems of traditional biological nitrogen and phosphorus removal, including long process duration and high infrastructural and operational costs, the simultaneous nitrogen and phosphorus removal capabilities, influencing factors and kinetic characteristics were systematically studied using the heterotrophic nitrifier Acinetobacter junii NP1 which possesses efficient simultaneous nitrogen and phosphorus removal ability. The results showed that strain NP1 exhibited efficient heterotrophic nitrification ability with a maximum ammonia removal rate of 99.12%. Furthermore, only small amounts of nitrification intermediates were accumulated during the reaction process. Strain NP1 also adapted well to higher ammonia nitrogen loading. In addition, strain NP1 had efficient aerobic denitrification characteristics, and could utilize nitrite and nitrate for growth and metabolism, achieving a maximum removal rate of 91.40% and 95.10%, respectively. The heterotrophic nitrification process of strain NP1 was accompanied by simultaneous phosphorus accumulation, and the appropriate ratio of nitrogen to phosphorus was beneficial for the simultaneous removal of nitrogen and phosphorus. When the ratio of nitrogen to phosphorus was 5:1, the maximum ammonia nitrogen and phosphate removal rates reached 99.21% and 88.35%, respectively. The bacterial growth process of stain NP1 matched the Logistic model (R2>0.99), and the nitrogen and phosphate degradation conformed to the Compertz model (R2>0.99). The maximum conversion rates of nitrogen and phosphate (Rm) obtained by model fitting were in the order ammonia>nitrate>nitrite, and lag time (t0) was in the order nitrate>nitrite>ammonia. According to the analysis of the degradation kinetics of the matrix and the removal rate of nitrogen and phosphorus, the optimal conditions were found to be sodium succinate, C/N=10, T=30℃, and r=160 r·min-1.
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Nitrogen Removal by Heterotrophic Nitrifying Bacterium Pseudomonas putida YH and Its Kinetic Characteristics
Huan jing ke xue= Huanjing kexue, 2019Co-Authors: Xu-hui Wang, Lei Yang, Ning Chen, Qian Xiao, Dan LiAbstract:Due to the low nitrogen removal efficiency, lengthy procedure, and vulnerability to high loads of ammonium of the traditional sewage treatment process, the physiological characteristics, nitrogen removal characteristics, influencing factors, and kinetic analysis were systematically studied for the heterotrophic nitrifier Pseudomonas putida YH, which has efficient nitrogen removal ability. The results showed that strain YH exhibited efficient heterotrophic nitrification ability with a low accumulation of nitrification products. The maximum ammonium removal rate was 99.1%, and nearly 53% of the removed TN was converted to intracellular nitrogen. In addition, nitrite and nitrate could also be metabolized by strain YH under aerobic conditions, with a maximum removal rate of 99.8% and 99.5%, respectively. Additionally, the successful PCR amplification of the napA and nirK genes further improved the aerobic denitrification characteristics of strain YH. The bacterial growth process of strain YH matched the Logistic model (R2>0.99), and the nitrogen degradation conformed to the Compertz model (R2>0.99). The order of the maximum conversion rates of nitrogen (Rm) was ammonia > nitrate > nitrite, and that of the lag time (t0) was nitrate > nitrite > ammonia. The optimal conditions for heterotrophic ammonium oxidation with strain YH were succinate as the carbon source, C/N=10, T=30℃, r=160-200 r·min-1, and pH=7.0. Under the optimal conditions, the average ammonia oxidation rate and Rm were 8.35 and 16.71 mg·(L·h)-1, respectively. Strain YH could adapt to a broad range of ammonium loads. A high ammonium removal rate was observed under high ammonium concentrations (1000 mg·L-1), indicating the high potential of strain YH for application in high-strength ammonium wastewater treatment.
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the characteristics of a novel heterotrophic Nitrifying and aerobic deNitrifying Bacterium acinetobacter junii yb
Bioresource Technology, 2014Co-Authors: Lei Yang, Xian LiangAbstract:Abstract A novel heterotrophic Nitrifying Bacterium was isolated from activated sludge and was identified as Acinetobacter junii YB. The strain exhibited efficient heterotrophic nitrification–aerobic denitrification ability at a broad range of ammonium loads and had the capability to utilize hydroxylamine, nitrite and nitrate as a sole nitrogen source. Based on the nitrogen removal and enzyme assay, the nitrogen removal pathway was speculated to be achieved through heterotrophic nitrification coupled with aerobic denitrification. In addition, single-factor experiments showed that efficient heterotrophic nitrification and growth of strain YB occurred with succinate as the carbon source, pH 7.5, 37 °C, and high C/N ratio and dissolved oxygen. Furthermore, the new isolate showed capacities for aggregation and hydrophobicity. Regular variations of the flocculating ability and relative hydrophobicity were observed during the whole cultivation. The ability to perform heterotrophic nitrification–aerobic denitrification and cell aggregation demonstrated the great potential of the strain YB for future applications.