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Denise Cunha Fernandes Dos Santos Dias - One of the best experts on this subject based on the ideXlab platform.
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Effect of cyanide by sodium Nitroprusside (SNP) application on germination, antioxidative system and lipid peroxidation of Senna macranthera seeds under saline stress
2019Co-Authors: Aparecida Leonir Da Silva, Daniel Teixeira Pinheiro, Eduardo Euclydes De Lima E Borges, Laércio Junio Da Silva, Denise Cunha Fernandes Dos Santos DiasAbstract:Abstract: The effects of NO donors on germination under saline stress have been much investigated for many species, however, there are reports that the effect caused by donors are effects of cyanide present. The aim of this study was to evaluate the effects of sodium Nitroprusside (SNP) on germination, antioxidative system and lipid peroxidation of Senna macranthera seeds under saline stress. The osmotic potentials of -0.4 and -0.5 MPa of NaCl were used, as well as the concentration of 100 μM of sodium Nitroprusside, inactive sodium Nitroprusside. Germination rate, imbibition curves, antioxidant enzyme activity, lipid peroxidation, and protein content were evaluated. Similar effects were observed for sodium Nitroprusside and inactive sodium Nitroprusside, indicating that these effects were related to the release of the cyanide present in sodium Nitroprusside. The sodium Nitroprusside (SNP) improves the germination of Senna macranthera seeds under salt stress conditions, through release of cyanide. The cyanide favored germination, reducing lipid peroxidation and increasing the activity of the antioxidant enzymes.
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Salinity tolerance in Senna macranthera (DC. ex Collad.) H. S. Irwin & Barneby seeds with sodium Nitroprusside (SNP) promoted by cyanide
2018Co-Authors: Aparecida Leonir Da Silva, Daniel Teixeira Pinheiro, Eduardo Euclydes De Lima E Borges, Laércio Junio Da Silva, Denise Cunha Fernandes Dos Santos DiasAbstract:Abstract: Studies show the positive effect of the nitric oxide donor in the seed germination process. However, there are no precise reports about which compound present in sodium Nitroprusside would cause these effects in seeds under salt stress. The aim of this study was to evaluate the effects of SNP on germination of Senna macranthera seeds under salt stress. The osmotic potentials of -0.3, -0.4, and -0.5 MPa of NaCl were used, as well as the concentration of 100 μM of sodium Nitroprusside, inactive sodium Nitroprusside, and ferrocyanide. The sodium Nitroprusside in germination was applied before, during, and after salt stress. Germination rate, germination speed index, percentage of normal seedlings, and seedling development components were evaluated. The treatments that proved to be more promising in recovery of germination under salt stress are -0.4 MPa together with SNP; -0.4 MPa together with inactive SNP; -0.5 MPa together with SNP and -0.5 MPa together with inactive SNP. Effects similar to those brought about by the sodium Nitroprusside treatment were also observed in the treatments with inactive sodium Nitroprusside and ferrocyanide, indicating that the effects observed were related to release of the cyanide present in the sodium Nitroprusside.
Harshad Navsaria - One of the best experts on this subject based on the ideXlab platform.
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longitudinal muscle shows abnormal relaxation responses to nitric oxide and contains altered levels of nos1 and elastin in uncomplicated diverticular disease
Colorectal Disease, 2007Co-Authors: Mark Golder, David E Burleigh, Lucy Ghali, R M Feakins, Peter J Lunniss, N S Williams, Harshad NavsariaAbstract:OBJECTIVE: Recent evidence challenges the 'low-fibre/high-colonic intraluminal pressure' hypothesis of diverticular disease (DD) and raises the possibility that other mechanisms are involved. Although bowel wall smooth muscle is known to be hypercontractile in DD, the nature of its relaxation is unknown. The present study investigated colonic smooth muscle responses to nitric oxide, as well as the smooth muscle contents of neural nitric oxide and elastin associated with the disease. METHOD: Immunohistochemical/image analysis of antibodies to nitric oxide synthase (NOS1), co-localized with protein gene product (PGP) and to elastin, was performed on three histological sections of sigmoid colons from 20 patients (10 DD, 10 controls) following resections for rectal tumours. Organ bath experiments examined smooth muscle responsiveness to Nitroprusside, a nitric oxide donor. RESULTS: Uncomplicated diverticular longitudinal muscle showed lower nitric oxide immunoreactivity compared with controls: median percentage surface area of NOS1 over PGP was 26.0% (range 0.5-58.3), controls 45.0% (35.0-70.1; P = 0.018). Median percentage surface area of elastin was elevated, 21.3% (10.6-45.6), controls 8.2% (1.7-13.5; P = 0.0002), together with a low sensitivity to Nitroprusside [mean - log EC(50) 5.3 (SD 0.5), controls 6.6 (SD 0.5), difference 1.3, 95% CI 0.8-1.7; P < 0.0001] and there were lower maximum relaxation responses to Nitroprusside compared with controls: median percentage (relaxation induced by nitroprussside/contraction induced by bethanecol) was 52.0%, range (20.0-92.0), controls 100.0% (71.0-125.0), P < 0.0001. No statistically significant differences were found in circular muscle, at the sample size studied. CONCLUSION: This study established, for the first time, specific abnormalities in longitudinal muscle relaxation and contents of neural nitric oxide and elastin in uncomplicated DD. These findings may have important implications for both colon structure and function in the disease.
Aparecida Leonir Da Silva - One of the best experts on this subject based on the ideXlab platform.
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Effect of cyanide by sodium Nitroprusside (SNP) application on germination, antioxidative system and lipid peroxidation of Senna macranthera seeds under saline stress
2019Co-Authors: Aparecida Leonir Da Silva, Daniel Teixeira Pinheiro, Eduardo Euclydes De Lima E Borges, Laércio Junio Da Silva, Denise Cunha Fernandes Dos Santos DiasAbstract:Abstract: The effects of NO donors on germination under saline stress have been much investigated for many species, however, there are reports that the effect caused by donors are effects of cyanide present. The aim of this study was to evaluate the effects of sodium Nitroprusside (SNP) on germination, antioxidative system and lipid peroxidation of Senna macranthera seeds under saline stress. The osmotic potentials of -0.4 and -0.5 MPa of NaCl were used, as well as the concentration of 100 μM of sodium Nitroprusside, inactive sodium Nitroprusside. Germination rate, imbibition curves, antioxidant enzyme activity, lipid peroxidation, and protein content were evaluated. Similar effects were observed for sodium Nitroprusside and inactive sodium Nitroprusside, indicating that these effects were related to the release of the cyanide present in sodium Nitroprusside. The sodium Nitroprusside (SNP) improves the germination of Senna macranthera seeds under salt stress conditions, through release of cyanide. The cyanide favored germination, reducing lipid peroxidation and increasing the activity of the antioxidant enzymes.
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Salinity tolerance in Senna macranthera (DC. ex Collad.) H. S. Irwin & Barneby seeds with sodium Nitroprusside (SNP) promoted by cyanide
2018Co-Authors: Aparecida Leonir Da Silva, Daniel Teixeira Pinheiro, Eduardo Euclydes De Lima E Borges, Laércio Junio Da Silva, Denise Cunha Fernandes Dos Santos DiasAbstract:Abstract: Studies show the positive effect of the nitric oxide donor in the seed germination process. However, there are no precise reports about which compound present in sodium Nitroprusside would cause these effects in seeds under salt stress. The aim of this study was to evaluate the effects of SNP on germination of Senna macranthera seeds under salt stress. The osmotic potentials of -0.3, -0.4, and -0.5 MPa of NaCl were used, as well as the concentration of 100 μM of sodium Nitroprusside, inactive sodium Nitroprusside, and ferrocyanide. The sodium Nitroprusside in germination was applied before, during, and after salt stress. Germination rate, germination speed index, percentage of normal seedlings, and seedling development components were evaluated. The treatments that proved to be more promising in recovery of germination under salt stress are -0.4 MPa together with SNP; -0.4 MPa together with inactive SNP; -0.5 MPa together with SNP and -0.5 MPa together with inactive SNP. Effects similar to those brought about by the sodium Nitroprusside treatment were also observed in the treatments with inactive sodium Nitroprusside and ferrocyanide, indicating that the effects observed were related to release of the cyanide present in the sodium Nitroprusside.
Edward P. Sloane - One of the best experts on this subject based on the ideXlab platform.
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Cyanide Toxicity from Sodium Nitroprusside: Risks and Management
The Annals of pharmacotherapy, 1992Co-Authors: Joseph P. Rindone, Edward P. SloaneAbstract:OBJECTIVE:To review the risks, manifestations, and treatment of cyanide toxicity from Nitroprusside therapy.DATA SOURCES:All English case reports identified in Index Medicus (MEDLINE) of cyanide intoxication related to Nitroprusside from 1970 to the present were reviewed. In addition, literature regarding the incidence, risks, and treatment of cyanide toxicity from Nitroprusside is presented.CONCLUSIONS:Numerous cases of cyanide toxicity associated with Nitroprusside have been reported. The overall incidence appears to be infrequent; however, certain patients may be at high risk. Risk factors may include hypoalbuminemia, cardiopulmonary bypass procedures, or the administration of moderate to high doses of Nitroprusside. Treatment of cyanide toxicity requires the cessation of Nitroprusside and, for severe toxicity, use of the cyanide antidote kit. Cyanide toxicity from Nitroprusside may be prevented by concomitant administration of sodium thiosulfate infusions.
D G Harrison - One of the best experts on this subject based on the ideXlab platform.
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Nitric oxide generation from Nitroprusside by vascular tissue. Evidence that reduction of the Nitroprusside anion and cyanide loss are required.
Biochemical pharmacology, 1991Co-Authors: J N Bates, M T Baker, R Guerra, D G HarrisonAbstract:Nitric oxide (NO) was produced from sodium Nitroprusside in the presence of vascular tissue but was not released spontaneously from the Nitroprusside anion. In the absence of tissue in the dark Nitroprusside did not release NO. When solutions of Nitroprusside alone were irradiated with visible light, nitric oxide was released at rates linearly proportional to Nitroprusside concentration and light intensity. Nitric oxide was produced from solutions of Nitroprusside in the dark after the addition of vascular tissue, including lengths of rabbit aorta, subcellular fractions of aorta, and human plasma. NO was also released from Nitroprusside after reaction with various reducing agents including cysteine and other thiols, ascorbic acid, sodium dithionite, ferrous chloride, hemoglobin, myoglobin, and partially purified cytochrome P450 with an NADPH-regenerating system. HCN was simultaneously produced in these solutions, and addition of KCN blocked NO release. Iodine oxidized intermediate cyanoferrates and blocked nitric oxide release. KCN or iodine also blocked NO production by tissue, but had no effect upon photochemical NO release. These results show that, apart from photolysis which makes no physiological contribution, release of nitric oxide from Nitroprusside, in simple solutions and in biological tissue, occurs after Nitroprusside has undergone reduction and lost cyanide.